Restriction map for
pFN21AB8151
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# NO CUT ENZYMES ( 131 enzymes) #
##################################
AatII Acc65I AccI AccIII AcyI
AflII AgeI AhdI Alw44I AocI
Aor51HI ApaLI AscI Asp718I AspEI
AspI AvaII AviII AvrII BalI
BamHI BbeI BbrPI BbsI BbvCI
BcgI BciVI BclI BfaI BfrI
BglI BlnI BpuAI BsaAI BsaBI
BsaHI BsaI BsaWI BseAI BsgI
BsiEI BsiWI Bsp1407I BspDI BspEI
BspHI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstXI BstZ17I Bsu36I
ClaI CpoI DraI DrdI DsaI
EaeI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoRV EcoT22I
FbaI FseI FspI Hin1I HindIII
HpaI KasI KpnI KspI MaeI
MamI MfeI MluI MluNI MroI
MscI MslI MunI NaeI NarI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PflFI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PvuI PvuII RcaI RsrII
SacI SacII SalI SbfI ScaI
SexAI SfaNI SfiI SfoI SfuI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI Tth111I XbaI
XcmI
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# ONE CUT ENZYMES ( 51 enzymes) #
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AclI 336 AACGTT AA^CGTT
AcsI 304 GAATTC [GA]^AATT[CT]
AfaI 39 GTAC GT^AC
AflIII 867 ACATGT A^C[GA][CT]GT
AlwNI 631 CAGCCACTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 304 GAATTC [GA]^AATT[CT]
AseI 805 ATTAAT AT^TAAT
AsnI 805 ATTAAT AT^TAAT
Asp700I 445 GAAGATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BanI 210 GGTGCC G^G[CT][GA]CC
BglII 447 AGATCT A^GATCT
BlpI 144 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 144 GCTCAGC GC^T[ACGT]AGC
BsmI 510 GAATGC GAATGC(1/-1)
BspLU11I 867 ACATGT A^CATGT
BspMI 573 ACCTGC ACCTGC(4/8)
BsrDI 287 CATTGC GCAATG(2/0)
BsrFI 207 GCCGGT [GA]^CCGG[CT]
BstEII 607 GGTAACC G^GT[ACGT]ACC
BstF5I 539 GGATG GGATG(2/0)
BstPI 607 GGTAACC G^GT[ACGT]ACC
CelII 144 GCTCAGC GC^T[ACGT]AGC
Cfr10I 207 GCCGGT [GA]^CCGG[CT]
DraII 655 GGGCCCT [GA]G^G[ACGT]CC[CT]
EarI 496 GAAGAG CTCTTC(1/4)
EcoO109I 655 GGGCCCT [GA]G^G[ACGT]CC[CT]
EcoO65I 607 GGTAACC G^GT[ACGT]ACC
EcoRI 304 GAATTC G^AATTC
EcoT14I 764 CCTTGG C^C[AT][AT]GG
FokI 539 GGATG GGATG(9/13)
HaeII 72 GGCGCT [GA]GCGC^[CT]
HincII 676 GTCAAC GT[CT]^[GA]AC
HindII 676 GTCAAC GT[CT]^[GA]AC
Ksp632I 496 GAAGAG CTCTTC(1/4)
NlaIII 868 CATG CATG^
NspI 867 ACATGT [GA]CATG^[CT]
PaeR7I 117 CTCGAG C^TCGAG
PflMI 634 CCACTGGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshBI 805 ATTAAT AT^TAAT
Psp1406I 336 AACGTT AA^CGTT
PstI 79 CTGCAG CTGCA^G
RsaI 39 GTAC GT^AC
SapI 496 GAAGAGC GCTCTTC(1/4)
SfcI 79 CTGCAG C^T[GA][CT]AG
SgrAI 206 CGCCGGTG C[GA]^CCGG[CT]G
StyI 764 CCTTGG C^C[AT][AT]GG
Tsp45I 851 GTCAC ^GT[GC]AC
Tsp509I 305 AATT ^AATT
Van91I 634 CCACTGGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 117 CTCGAG C^TCGAG
XmnI 445 GAAGATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AluI 34 AGCT AG^CT
785 AGCT
AlwI 359 GATCC GGATC(4/5)
882 GGATC
ApaI 655 GGGCCC GGGCC^C
706 GGGCCC
AspHI 120 GAGCAC G[AT]GC[AT]^C
142 GTGCTC
BpmI 252 CTCCAG CTGGAG(16/14)
697 CTCCAG
BseRI 22 GAGGAG GAGGAG(10/8)
394 GAGGAG
BsiHKAI 120 GAGCAC G[AT]GC[AT]^C
142 GTGCTC
BsmBI 150 CGTCTC CGTCTC(1/5)
168 CGTCTC
BsrBI 685 CCGCTC CCGCTC(-3/-3)
25 GAGCGG
BstYI 358 AGATCC [GA]^GATC[CT]
447 AGATCT
DraIII 94 CACGGGGTG CAC[ACGT][ACGT][ACGT]^GTG
635 CACTGGGTG
HphI 243 GGTGA GGTGA(8/7)
781 GGTGA
MaeII 337 ACGT A^CGT
557 ACGT
MflI 358 AGATCC [GA]^GATC[CT]
447 AGATCT
MspA1I 88 CAGCGG C[AC]G^C[GT]G
192 CAGCGG
SmaI 130 CCCGGG CCC^GGG
238 CCCGGG
SmlI 117 CTCGAG C^T[CT][GA]AG
474 CTTGAG
XhoII 358 AGATCC [GA]^GATC[CT]
447 AGATCT
XmaCI 130 CCCGGG C^CCGGG
238 CCCGGG
XmaI 130 CCCGGG C^CCGGG
238 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 56 enzymes) #
#########################################
AccII AciI AvaI BanII BbvI
BcnI BmyI BsaJI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BsrI
BssKI BstNI BstUI Cac8I CfoI
Cfr13I DdeI DpnII EcoRII FauI
Fnu4HI HaeIII HapII HgaI HhaI
HinP1I HinfI HpaII ItaI MaeIII
MboI MboII MnlI MseI MspI
MvaI MvnI MwoI NciI NdeII
NlaIV PleI Sau3AI Sau96I ScrFI
TaqI TfiI Tru9I TseI TspRI
TthHB8I