Restriction map for
pFN21AB8151
(Circular) (Six-base) MAPSORT of: pFN21AB8151.seq Check: 5210 from: 1 to: 5617
pFN21AB8151 5617 bp
With 182 enzymes: SgfI *
October 20, 2009 10:50 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3427 278
Size: 53 83 186 936 1891 2468
Fragments arranged by size:
2468 1891 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2960 1937
Size: 1023 4594
Acc65I G'GTAC_C
Cuts at: 2938 2938
Size: 5617
AclI AA'CG_TT
Cuts at: 1563 2342 3744 4117 1563
Size: 779 1402 373 3063
Fragments arranged by size:
3063 1402 779 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2520 2896 3680 5064 5332 1632
Size: 888 376 784 1384 268 1917
Fragments arranged by size:
1917 1384 888 784 376 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5420
AflIII A'CryG_T
Cuts at: 1203 2872 4516 5493 1203
Size: 1669 1644 977 1327
Fragments arranged by size:
1669 1644 1327 977
AgeI A'CCGG_T
Cuts at: 4448 4448
Size: 5617
AhdI GACnn_n'nnGTC
Cuts at: 4346 4346
Size: 5617
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5617
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5617
AlwNI CAG_nnn'CTG
Cuts at: 2641 3360 4932 2641
Size: 719 1572 3326
Fragments arranged by size:
3326 1572 719
ApaI G_GGCC'C
Cuts at: 2664 2715 2664
Size: 51 5566
ApaLI G'TGCA_C
Cuts at: 3674 4830 3674
Size: 1156 4461
ApoI r'AATT_y
Cuts at: 1493 2309 2926 3152 3304 5413 1493
Size: 816 617 226 152 2109 1697
Fragments arranged by size:
2109 1697 816 617 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2811 4171 160
Size: 872 1779 1360 1606
Fragments arranged by size:
1779 1606 1360 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5617
AvaI C'yCGr_G
Cuts at: 1870 1943 2122 2135 2243 2942 1870
Size: 73 179 13 108 699 4545
Fragments arranged by size:
4545 699 179 108 73 13
BamHI G'GATC_C
Cuts at: 2947 3314 2947
Size: 367 5250
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2215 2938 4393 618
Size: 325 217 627 428 723 1455 1842
Fragments arranged by size:
1842 1455 723 627 428 325 217
BanII G_rGCy'C
Cuts at: 1391 2255 2422 2664 2715 2936 1391
Size: 864 167 242 51 221 4072
Fragments arranged by size:
4072 864 242 221 167 51
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4646
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3808 3842 1903
Size: 34 1871 34 3678
Fragments arranged by size:
3678 1871 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3509 4719 3509
Size: 1210 4407
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5617
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4228 136
Size: 107 122 71 631 605 2556 1525
Fragments arranged by size:
2556 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2452 5612 2452
Size: 3160 2457
BlpI GC'TnA_GC
Cuts at: 2150 3034 2150
Size: 884 4733
Bme1580I G_kGCm'C
Cuts at: 1790 2664 2715 3678 4834 1790
Size: 874 51 963 1156 2573
Fragments arranged by size:
2573 1156 963 874 51
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5617
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2056 2651 3348
Size: 416 53 83 399 24 639 595 697
Cuts at: 3348 4306 442
Size: 958 1753
Fragments arranged by size:
1753 958 697 639 595 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5617
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2405 2405
Size: 5617
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2242 2687 4277 1239
Size: 1003 445 1590 2579
Fragments arranged by size:
2579 1590 1003 445
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2500 3742 4607 4905 2500
Size: 1242 865 298 3212
Fragments arranged by size:
3212 1242 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4280 882
Size: 617 2781 2219
Fragments arranged by size:
2781 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3408 493
Size: 711 2204 2702
Fragments arranged by size:
2702 2204 711
BsaBI GATnn'nnATC
Cuts at: 5611 5611
Size: 5617
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3424 3806 4441 275
Size: 53 83 186 936 1891 382 635 1451
Fragments arranged by size:
1891 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4050 4448 4722 4869 5280 4050
Size: 398 274 147 411 4387
Fragments arranged by size:
4387 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2393 2423 1178
Size: 30 165 30 990 30 4372
Fragments arranged by size:
4372 990 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2042 2414 1186
Size: 856 372 4389
Fragments arranged by size:
4389 856 372
BseYI C'CCAG_C
Cuts at: 2232 2675 4820 5316 2232
Size: 443 2145 496 2533
Fragments arranged by size:
2533 2145 496 443
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5044
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3067 3828 4856 1540
Size: 275 186 1066 761 1028 2301
Fragments arranged by size:
2301 1066 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2129 2151 3678 3763 4834 2129
Size: 22 1527 85 1071 2912
Fragments arranged by size:
2912 1527 1071 85 22
BsmI GAATG_Cn'
Cuts at: 2521 3128 3221 2521
Size: 607 93 4917
Fragments arranged by size:
4917 607 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2161 2179 2161
Size: 18 5599
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2129 2151 2255 2422 2664 2715 2936
Size: 399 339 22 104 167 242 51 221
Cuts at: 2936 3678 3763 4834 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 339 242 221 167
104 85 51 22
BspEI T'CCGG_A
Cuts at: 5280 5280
Size: 5617
BspHI T'CATG_A
Cuts at: 3506 5236 3506
Size: 1730 3887
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2587 2972 844
Size: 1743 385 3489
Fragments arranged by size:
3489 1743 385
BsrBI CCG'CTC
Cuts at: 1394 1823 2032 2692 3504 1394
Size: 429 209 660 812 3507
Fragments arranged by size:
3507 812 660 429 209
BsrDI GCAATG_nn'
Cuts at: 65 1248 2291 4112 4286 65
Size: 1183 1043 1821 174 1396
Fragments arranged by size:
1821 1396 1183 1043 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2212 4261 4448 1700
Size: 110 402 2049 187 2869
Fragments arranged by size:
2869 2049 402 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5617
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5617
BssSI C'ACGA_G
Cuts at: 3677 4689 3677
Size: 1012 4605
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5617
BstBI TT'CG_AA
Cuts at: 4421 4421
Size: 5617
BstEII G'GTnAC_C
Cuts at: 1184 2612 1184
Size: 1428 4189
BstYI r'GATC_y
Cuts at: 1972 2363 2452 2947 3314 3699 3716 5168 5254
Size: 391 89 495 367 385 17 1452 86
Cuts at: 5254 5266 5612 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 495 391 385 367 346 89
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4126
Fragments arranged by size:
4126 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4878
Fragments arranged by size:
4878 683 56
BtsI GCAGTG_nn'
Cuts at: 2513 3133 3927 3947 2513
Size: 620 794 20 4183
Fragments arranged by size:
4183 794 620 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5617
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5582
DraI TTT'AAA
Cuts at: 2921 2921
Size: 5617
DraIII CAC_nnn'GTG
Cuts at: 2104 2645 2104
Size: 541 5076
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4624 809
Size: 507 3308 1802
Fragments arranged by size:
3308 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3064 3953 5582
Size: 54 1425 50 275 18 1234 889 1629
Cuts at: 5582 8
Size: 43
Fragments arranged by size:
1629 1425 1234 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3064 1537
Size: 275 1252 4090
Fragments arranged by size:
4090 1252 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2496 3547 1353
Size: 1143 1051 3423
Fragments arranged by size:
3423 1143 1051
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2229 2420 2575 4204 4578 4724
Size: 1064 138 640 191 155 1629 374 146
Cuts at: 4724 387
Size: 1280
Fragments arranged by size:
1629 1280 1064 640 374 191 155 146
138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2242 2520 2687 2896 3680 4277 5064
Size: 393 610 278 167 209 784 597 787
Cuts at: 5064 5332 1239
Size: 268 1524
Fragments arranged by size:
1524 787 784 610 597 393 278 268
209 167
EcoO109I rG'GnC_Cy
Cuts at: 2661 2661
Size: 5617
EcoRI G'AATT_C
Cuts at: 2309 2926 3304 2309
Size: 617 378 4622
Fragments arranged by size:
4622 617 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2785 2785
Size: 5617
FspI TGC'GCA
Cuts at: 4123 4123
Size: 5617
HaeII r_GCGC'y
Cuts at: 1678 2081 4764 1678
Size: 403 2683 2531
Fragments arranged by size:
2683 2531 403
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4258 4290 4332 4364 1373
Size: 32 135 32 2686 32 42 32 2626
Fragments arranged by size:
2686 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2683 2961 3207 669
Size: 988 281 745 278 246 3079
Fragments arranged by size:
3079 988 745 281 278 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5617
HpaI GTT'AAC
Cuts at: 3207 3207
Size: 5617
KpnI G_GTAC'C
Cuts at: 2942 2942
Size: 5617
MfeI C'AATT_G
Cuts at: 3216 3216
Size: 5617
MluI A'CGCG_T
Cuts at: 5493 5493
Size: 5617
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4731 4915 1305
Size: 3426 184 2007
Fragments arranged by size:
3426 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3795
Fragments arranged by size:
3795 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3575 3934 4093 518
Size: 648 2409 359 159 2042
Fragments arranged by size:
2409 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2095 2199 3033 3710 4858 5103 1606
Size: 489 104 834 677 1148 245 2120
Fragments arranged by size:
2120 1148 834 677 489 245 104
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4126
Fragments arranged by size:
4126 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5617
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5617
NotI GC'GGCC_GC
Cuts at: 3064 3064
Size: 5617
NspI r_CATG'y
Cuts at: 1132 1229 2876 2975 4520 1132
Size: 97 1647 99 1545 2229
Fragments arranged by size:
2229 1647 1545 99 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5617
PciI A'CATG_T
Cuts at: 2872 4516 2872
Size: 1644 3973
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2645 1108
Size: 1537 4080
PfoI T'CCnGG_A
Cuts at: 2487 2487
Size: 5617
PmeI GTTT'AAAC
Cuts at: 2921 2921
Size: 5617
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3686 3718 5199 5231 3686
Size: 32 1481 32 4072
Fragments arranged by size:
4072 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5617
PsiI TTA'TAA
Cuts at: 3187 3187
Size: 5617
PspOMI G'GGCC_C
Cuts at: 2660 2711 2660
Size: 51 5566
PspXI vC'TCGA_Gb
Cuts at: 2122 2122
Size: 5617
PstI C_TGCA'G
Cuts at: 830 2088 2969 830
Size: 1258 881 3478
Fragments arranged by size:
3478 1258 881
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5617
SalI G'TCGA_C
Cuts at: 1936 2959 1936
Size: 1023 4594
SapI GCTCTTCn'nnn_
Cuts at: 2496 2496
Size: 5617
SbfI CC_TGCA'GG
Cuts at: 2969 2969
Size: 5617
ScaI AGT'ACT
Cuts at: 3865 5577 3865
Size: 1712 3905
SfcI C'TryA_G
Cuts at: 826 1045 2084 2965 4100 4781 4972 826
Size: 219 1039 881 1135 681 191 1471
Fragments arranged by size:
1471 1135 1039 881 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5617
SgrAI Cr'CCGG_yG
Cuts at: 1700 2212 1700
Size: 512 5105
SmaI CCC'GGG
Cuts at: 1872 2137 2245 2944 1872
Size: 265 108 699 4545
Fragments arranged by size:
4545 699 265 108
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2122 2479 3721 4622 4884 820
Size: 197 926 179 357 1242 901 262 1553
Fragments arranged by size:
1553 1242 926 901 357 262 197 179
SnaBI TAC'GTA
Cuts at: 493 3408 493
Size: 2915 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5617
SphI G_CATG'C
Cuts at: 1132 2975 1132
Size: 1843 3774
SspI AAT'ATT
Cuts at: 5 52 3541 5
Size: 47 3489 2081
Fragments arranged by size:
3489 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5617
StyI C'CwwG_G
Cuts at: 513 1065 2004 2769 3056 513
Size: 552 939 765 287 3074
Fragments arranged by size:
3074 939 765 552 287
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2633 2646 3644 3661 3814 3999 4444
Size: 1255 1053 13 998 17 153 185 445
Cuts at: 4444 325
Size: 1498
Fragments arranged by size:
1498 1255 1053 998 445 185 153 17
13
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3863 5575 96
Size: 274 80 33 51 1443 1886 1712 138
Fragments arranged by size:
1886 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2822 2854 5577 5609 2822
Size: 32 2723 32 2830
Fragments arranged by size:
2830 2723 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5617
XbaI T'CTAG_A
Cuts at: 2953 2953
Size: 5617
XhoI C'TCGA_G
Cuts at: 1943 2122 1943
Size: 179 5438
XmaI C'CCGG_G
Cuts at: 1870 2135 2243 2942 1870
Size: 265 108 699 4545
Fragments arranged by size:
4545 699 265 108
XmnI GAAnn'nnTTC
Cuts at: 2454 3746 2454
Size: 1292 4325
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3425 276
Size: 53 83 186 936 1891 2468
Fragments arranged by size:
2468 1891 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI
PspOMI PspXI PstI PvuI SalI SapI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BfrBI Bpu10I BsiWI BstXI BstZ17I Bsu36I EcoICRI EcoNI
EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PmlI PpuMI PsrI PvuII RsrII SacI
SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI