################################## # NO CUT ENZYMES ( 192 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcyI AfaI AflII AflIII AgeI AhdI Alw44I AlwNI AocI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AviII AvrII BalI BamHI BanI BbeI BbrPI BbsI BbvCI BcgI BciVI BfaI BfrI BglI BglII BlnI BlpI BpmI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BsaWI BseAI BseRI BsgI BsiEI BsiWI BsiYI BslI BsmAI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BsrI BssSI Bst1107I BstAPI BstBI BstEII BstPI BstXI BstYI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DdeI DraI DraIII DrdI DsaI EaeI EagI Eam1105I EarI EclXI Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FauI FseI FspI Hin1I HincII HindII HindIII HinfI HpaI HphI KasI KpnI Ksp632I KspI MaeI MaeII MaeIII MamI MfeI MflI MluI MluNI MroI MscI MseI MslI MunI NaeI NarI NcoI NdeI NgoMIV NheI NlaIII NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsaI RsrII SacII SalI SapI SbfI ScaI SexAI SfaNI SfcI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TfiI Tru9I Tsp45I Tth111I Van91I XbaI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 30 enzymes) # ################################### AlwI 277 GATCC GGATC(4/5) Aor51HI 106 AGCGCT AGC^GCT ApaI 94 GGGCCC GGGCC^C BclI 185 TGATCA T^GATCA BcnI 34 CCCGG CC^[GC]GG BsmFI 31 GTCCC GGGAC(10/14) BssHII 56 GCGCGC G^CGCGC BstNI 91 CCAGG CC^[AT]GG DraII 93 AGGGCCC [GA]G^G[ACGT]CC[CT] Eco47III 106 AGCGCT AGC^GCT EcoO109I 93 AGGGCCC [GA]G^G[ACGT]CC[CT] EcoRII 91 CCAGG ^CC[AT]GG EcoT14I 227 CCAAGG C^C[AT][AT]GG FbaI 185 TGATCA T^GATCA HaeII 106 AGCGCT [GA]GCGC^[CT] HapII 35 CCGG C^CGG HgaI 84 GACGC GACGC(5/10) HpaII 35 CCGG C^CGG MboII 252 GAAGA GAAGA(8/7) MspA1I 119 CCGCTG C[AC]G^C[GT]G MspI 35 CCGG C^CGG MvaI 91 CCAGG CC^[AT]GG NciI 34 CCCGG CC^[GC]GG NlaIV 94 GGGCCC GG[ACGT]^[ACGT]CC SacI 45 GAGCTC GAGCT^C StyI 227 CCAAGG C^C[AT][AT]GG TaqI 43 TCGA T^CGA TspRI 220 CAGTG CA[GC]TG[ACGT][ACGT]^ TthHB8I 43 TCGA T^CGA XcmI 227 CCAAGGTGGAAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 18 enzymes) # ################################### AcsI 266 AAATTT [GA]^AATT[CT] 314 AAATTT AluI 46 AGCT AG^CT 137 AGCT ApoI 266 AAATTT [GA]^AATT[CT] 314 AAATTT AspHI 45 GAGCTC G[AT]GC[AT]^C 222 GTGCTC AvaII 37 GGTCC G^G[AT]CC 112 GGTCC BanII 45 GAGCTC G[GA]GC[CT]^C 94 GGGCCC BsiHKAI 45 GAGCTC G[AT]GC[AT]^C 222 GTGCTC BssKI 34 CCCGG ^CC[ACGT]GG 91 CCAGG BstF5I 175 CATCC GGATG(2/0) 306 CATCC Cac8I 56 GCGCGC GC[ACGT]^[ACGT]GC 103 GCCAGC DpnII 186 GATC ^GATC 277 GATC FokI 175 CATCC GGATG(9/13) 306 CATCC MboI 186 GATC ^GATC 277 GATC MnlI 6 CCTC CCTC(7/6) 245 CCTC NdeII 186 GATC ^GATC 277 GATC Sau3AI 186 GATC ^GATC 277 GATC ScrFI 34 CCCGG CC^[ACGT]GG 91 CCAGG Tsp509I 267 AATT ^AATT 315 AATT ######################################### # 3 OR MORE CUT ENZYMES ( 18 enzymes) # ######################################### AccII AciI BbvI BmyI BsaJI Bsp1286I BstUI CfoI Cfr13I Fnu4HI HaeIII HhaI HinP1I ItaI MvnI MwoI Sau96I TseI