Restriction map for
pFN21AE5224
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# NO CUT ENZYMES ( 157 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
AhdI AlwNI AocI Aor51HI ApaI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AviII AvrII BalI
BamHI BanI BbeI BcgI BclI
BfaI BfrI BglI BlnI BlpI
BpmI Bpu1102I BsaBI BsaHI BseAI
BsiEI BsiWI BsmBI BsmI BspDI
BspEI BspHI BspLU11I BsrBI BsrDI
BsrFI BssHII Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstXI BstZ17I
Bsu36I CelII Cfr10I ClaI CpoI
DraI DraII DraIII DrdI DsaI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO109I EcoO65I EcoRV
EcoT14I EcoT22I FbaI FseI FspI
HaeII Hin1I HincII HindII HpaI
HphI KasI KpnI KspI MaeI
MaeIII MamI MfeI MluI MluNI
MroI MscI MseI MslI MunI
MvnI MwoI NaeI NarI NcoI
NgoMIV NheI NlaIV NotI NruI
NsiI NspI NspV PacI PflFI
PinAI PmeI PpuMI PshAI PshBI
Psp1406I PvuI PvuII RcaI RsrII
SacII SalI SapI SbfI ScaI
SfaNI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspI StuI StyI SwaI Tru9I
Tsp45I Tth111I XbaI XcmI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 52 enzymes) #
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AcsI 369 GAATTC [GA]^AATT[CT]
AfaI 192 GTAC GT^AC
Alw44I 400 GTGCAC G^TGCAC
ApaLI 400 GTGCAC G^TGCAC
ApoI 369 GAATTC [GA]^AATT[CT]
AvaI 36 CTCGAG C^[CT]CG[GA]G
AvaII 136 GGACC G^G[AT]CC
BanII 469 GAGCTC G[GA]GC[CT]^C
BbsI 496 GAAGAC GAAGAC(2/6)
BbvCI 75 CCTCAGC CCTCAGC(-5/-2)
BciVI 482 GTATCC GTATCC(6/5)
BcnI 278 CCCGG CC^[GC]GG
BglII 363 AGATCT A^GATCT
BpuAI 496 GAAGAC GAAGAC(2/6)
BsaI 385 GAGACC GGTCTC(1/5)
BsaWI 50 ACCGGA [AT]^CCGG[AT]
BsgI 562 CTGCAC GTGCAG(16/14)
BsmAI 385 GAGAC GTCTC(1/5)
BsoBI 36 CTCGAG C^[CT]CG[GA]G
Bsp1407I 191 TGTACA T^GTACA
BspMI 388 ACCTGC ACCTGC(4/8)
BsrGI 191 TGTACA T^GTACA
BsrI 477 CCAGT ACTGG(1/-1)
BssSI 433 CACGAG CACGAG(-5/-1)
BstYI 363 AGATCT [GA]^GATC[CT]
Cac8I 392 GCCAGC GC[ACGT]^[ACGT]GC
CfoI 64 GCGC GCG^C
Cfr13I 136 GGACC G^G[ACGT]CC
EcoRI 369 GAATTC G^AATTC
HgaI 95 GCGTC GACGC(5/10)
HhaI 64 GCGC GCG^C
HinP1I 64 GCGC G^CGC
HindIII 127 AAGCTT A^AGCTT
MflI 363 AGATCT [GA]^GATC[CT]
MspA1I 13 CCGCTG C[AC]G^C[GT]G
NciI 278 CCCGG CC^[GC]GG
PaeR7I 36 CTCGAG C^TCGAG
PflMI 351 CCAGGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 117 GACTC GAGTC(4/5)
PstI 526 CTGCAG CTGCA^G
RsaI 192 GTAC GT^AC
SacI 469 GAGCTC GAGCT^C
Sau96I 136 GGACC G^G[ACGT]CC
SexAI 350 ACCAGGT A^CC[AT]GGT
SfcI 526 CTGCAG C^T[GA][CT]AG
SmlI 36 CTCGAG C^T[CT][GA]AG
SspBI 191 TGTACA T^GTACA
Tsp509I 370 AATT ^AATT
TspRI 478 CAGTG CA[GC]TG[ACGT][ACGT]^
Van91I 351 CCAGGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 36 CTCGAG C^TCGAG
XhoII 363 AGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 20 enzymes) #
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AlwI 101 GATCC GGATC(4/5)
321 GATCC
BbrPI 121 CACGTG CAC^GTG
403 CACGTG
BsaAI 121 CACGTG [CT]AC^GT[GA]
403 CACGTG
BseRI 436 GAGGAG GAGGAG(10/8)
538 GAGGAG
BsmFI 115 GGGAC GGGAC(10/14)
9 GTCCC
BstF5I 72 CATCC GGATG(2/0)
420 CATCC
DdeI 76 CTCAG C^T[ACGT]AG
205 CTTAG
EaeI 280 CGGCCA [CT]^GGCC[GA]
549 TGGCCG
EarI 334 GAAGAG CTCTTC(1/4)
543 GAAGAG
FauI 12 CCCGC CCCGC(4/6)
18 GCGGG
FokI 72 CATCC GGATG(9/13)
420 CATCC
HapII 51 CCGG C^CGG
279 CCGG
HpaII 51 CCGG C^CGG
279 CCGG
Ksp632I 334 GAAGAG CTCTTC(1/4)
543 GAAGAG
MaeII 122 ACGT A^CGT
404 ACGT
MspI 51 CCGG C^CGG
279 CCGG
NdeI 179 CATATG CA^TATG
247 CATATG
NlaIII 57 CATG CATG^
501 CATG
PmaCI 121 CACGTG CAC^GTG
403 CACGTG
PmlI 121 CACGTG CAC^GTG
403 CACGTG
#########################################
# 3 OR MORE CUT ENZYMES ( 29 enzymes) #
#########################################
AciI AluI AspHI BbvI BmyI
BsaJI BsiHKAI BsiYI BslI Bsp1286I
BssKI BstNI DpnII EcoRII Fnu4HI
HaeIII HinfI ItaI MboI MboII
MnlI MvaI NdeII Sau3AI ScrFI
TaqI TfiI TseI TthHB8I