(Circular) (Six-base) MAPSORT of: pFN21AE5224.seq Check: 7414 from: 1 to: 5287 pFN21AE5224 5287 bp With 182 enzymes: SgfI * January 30, 2014 17:15 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3097 278 Size: 53 83 186 936 1561 2468 Fragments arranged by size: 2468 1561 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2630 1937 Size: 693 4594 Acc65I G'GTAC_C Cuts at: 2608 2608 Size: 5287 AclI AA'CG_TT Cuts at: 1563 3414 3787 1563 Size: 1851 373 3063 Fragments arranged by size: 3063 1851 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3350 4734 5002 1632 Size: 1718 1384 268 1917 Fragments arranged by size: 1917 1718 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5090 AflIII A'CryG_T Cuts at: 1203 4186 5163 1203 Size: 2983 977 1327 Fragments arranged by size: 2983 1327 977 AgeI A'CCGG_T Cuts at: 4118 4118 Size: 5287 AhdI GACnn_n'nnGTC Cuts at: 4016 4016 Size: 5287 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5287 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2582 1184 Size: 1398 3889 AlwNI CAG_nnn'CTG Cuts at: 3030 4602 3030 Size: 1572 3715 ApaLI G'TGCA_C Cuts at: 2405 3344 4500 2405 Size: 939 1156 3192 Fragments arranged by size: 3192 1156 939 ApoI r'AATT_y Cuts at: 1493 2374 2596 2822 2974 5083 1493 Size: 881 222 226 152 2109 1697 Fragments arranged by size: 2109 1697 881 226 222 152 AseI AT'TA_AT Cuts at: 160 1032 3841 160 Size: 872 2809 1606 Fragments arranged by size: 2809 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5287 AvaI C'yCGr_G Cuts at: 1870 1943 2041 2612 1870 Size: 73 98 571 4545 Fragments arranged by size: 4545 571 98 73 BamHI G'GATC_C Cuts at: 2617 2984 2617 Size: 367 4920 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2608 4063 618 Size: 325 217 627 821 1455 1842 Fragments arranged by size: 1842 1455 821 627 325 217 BanII G_rGCy'C Cuts at: 1391 2478 2606 1391 Size: 1087 128 4072 Fragments arranged by size: 4072 1087 128 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2508 928 Size: 971 609 3707 Fragments arranged by size: 3707 971 609 BbvCI CC'TCA_GC Cuts at: 2081 2081 Size: 5287 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3478 3512 1903 Size: 34 50 34 1457 34 3678 Fragments arranged by size: 3678 1457 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2498 3179 4389 2498 Size: 681 1210 3396 Fragments arranged by size: 3396 1210 681 BclI T'GATC_A Cuts at: 1548 1548 Size: 5287 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3898 136 Size: 107 122 71 631 605 2226 1525 Fragments arranged by size: 2226 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2368 5282 2368 Size: 2914 2373 BlpI GC'TnA_GC Cuts at: 2704 2704 Size: 5287 Bme1580I G_kGCm'C Cuts at: 1790 2409 3348 4504 1790 Size: 619 939 1156 2573 Fragments arranged by size: 2573 1156 939 619 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5287 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3018 3976 442 Size: 416 53 83 399 24 1601 958 1753 Fragments arranged by size: 1753 1601 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5287 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3947 1239 Size: 2708 2579 Bpu10I CC'TnA_GC Cuts at: 2081 2081 Size: 5287 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3412 4277 4575 3412 Size: 865 298 4124 Fragments arranged by size: 4124 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2384 3950 882 Size: 617 885 1566 2219 Fragments arranged by size: 2219 1566 885 617 BsaAI yAC'GTr Cuts at: 493 1204 2128 2410 3078 493 Size: 711 924 282 668 2702 Fragments arranged by size: 2702 924 711 668 282 BsaBI GATnn'nnATC Cuts at: 5281 5281 Size: 5287 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3094 3476 4111 275 Size: 53 83 186 936 1561 382 635 1451 Fragments arranged by size: 1561 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2055 3720 4118 4392 4539 4950 2055 Size: 1665 398 274 147 411 2392 Fragments arranged by size: 2392 1665 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5062 Fragments arranged by size: 5062 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2456 2558 1186 Size: 1270 102 3915 Fragments arranged by size: 3915 1270 102 BseYI C'CCAG_C Cuts at: 4490 4986 4490 Size: 496 4791 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2552 1163 Size: 573 816 3898 Fragments arranged by size: 3898 816 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2737 3498 4526 1540 Size: 275 186 736 761 1028 2301 Fragments arranged by size: 2301 1028 761 736 275 186 BsiHKAI G_wGCw'C Cuts at: 2042 2409 2478 3348 3433 4504 2042 Size: 367 69 870 85 1071 2825 Fragments arranged by size: 2825 1071 870 367 85 69 BsmI GAATG_Cn' Cuts at: 2798 2891 2798 Size: 93 5194 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2042 2409 2478 2606 3348 3433 4504 Size: 399 252 367 69 128 742 85 1071 Cuts at: 4504 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 399 367 252 128 85 69 BspEI T'CCGG_A Cuts at: 4950 4950 Size: 5287 BspHI T'CATG_A Cuts at: 3176 4906 3176 Size: 1730 3557 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2402 2642 844 Size: 1558 240 3489 Fragments arranged by size: 3489 1558 240 BsrBI CCG'CTC Cuts at: 1394 1823 3174 1394 Size: 429 1351 3507 Fragments arranged by size: 3507 1351 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3782 3956 65 Size: 1183 2534 174 1396 Fragments arranged by size: 2534 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3931 4118 1700 Size: 110 2121 187 2869 Fragments arranged by size: 2869 2121 187 110 BsrGI T'GTAC_A Cuts at: 96 2196 96 Size: 2100 3187 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5287 BssSI C'ACGA_G Cuts at: 2438 3347 4359 2438 Size: 909 1012 3366 Fragments arranged by size: 3366 1012 909 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5287 BstBI TT'CG_AA Cuts at: 4091 4091 Size: 5287 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5287 BstYI r'GATC_y Cuts at: 1972 2368 2617 2984 3369 3386 4838 4924 4936 Size: 396 249 367 385 17 1452 86 12 Cuts at: 4936 5282 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 396 385 367 346 249 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 4735 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4548 Fragments arranged by size: 4548 683 56 BtsI GCAGTG_nn' Cuts at: 2803 3597 3617 2803 Size: 794 20 4473 Fragments arranged by size: 4473 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5287 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5252 DraI TTT'AAA Cuts at: 2591 2591 Size: 5287 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4294 809 Size: 507 2978 1802 Fragments arranged by size: 2978 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2285 2554 2734 Size: 54 1425 50 275 18 455 269 180 Cuts at: 2734 3623 5252 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 455 275 269 180 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2734 1537 Size: 275 922 4090 Fragments arranged by size: 4090 922 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2334 2543 3217 1353 Size: 981 209 674 3423 Fragments arranged by size: 3423 981 674 209 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3874 4248 4394 387 Size: 1064 138 2285 374 146 1280 Fragments arranged by size: 2285 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2476 2476 Size: 5287 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3350 3947 4734 5002 1239 Size: 393 1718 597 787 268 1524 Fragments arranged by size: 1718 1524 787 597 393 268 EcoRI G'AATT_C Cuts at: 2374 2596 2974 2374 Size: 222 378 4687 Fragments arranged by size: 4687 378 222 FspI TGC'GCA Cuts at: 3793 3793 Size: 5287 HaeII r_GCGC'y Cuts at: 1678 4434 1678 Size: 2756 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2085 2117 3928 3960 4002 Size: 32 135 32 513 32 1811 32 42 Cuts at: 4002 4034 1373 Size: 32 2626 Fragments arranged by size: 2626 1811 513 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2631 2877 669 Size: 988 281 693 246 3079 Fragments arranged by size: 3079 988 693 281 246 HindIII A'AGCT_T Cuts at: 748 2132 748 Size: 1384 3903 HpaI GTT'AAC Cuts at: 2877 2877 Size: 5287 KpnI G_GTAC'C Cuts at: 2612 2612 Size: 5287 MfeI C'AATT_G Cuts at: 2886 2886 Size: 5287 MluI A'CGCG_T Cuts at: 5163 5163 Size: 5287 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4401 4585 1305 Size: 3096 184 2007 Fragments arranged by size: 3096 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3465 Fragments arranged by size: 3465 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3245 3604 3763 518 Size: 648 2079 359 159 2042 Fragments arranged by size: 2079 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2020 2703 3380 4528 4773 1606 Size: 414 683 677 1148 245 2120 Fragments arranged by size: 2120 1148 683 677 414 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4735 NdeI CA'TA_TG Cuts at: 387 2185 2253 387 Size: 1798 68 3421 Fragments arranged by size: 3421 1798 68 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5287 NotI GC'GGCC_GC Cuts at: 2734 2734 Size: 5287 NspI r_CATG'y Cuts at: 1132 1229 2645 4190 1132 Size: 97 1416 1545 2229 Fragments arranged by size: 2229 1545 1416 97 PasI CC'CwG_GG Cuts at: 1348 2452 1348 Size: 1104 4183 PciI A'CATG_T Cuts at: 4186 4186 Size: 5287 PflMI CCAn_nnn'nTGG Cuts at: 1108 2362 1108 Size: 1254 4033 PfoI T'CCnGG_A Cuts at: 2137 2427 2137 Size: 290 4997 PmeI GTTT'AAAC Cuts at: 2591 2591 Size: 5287 PmlI CAC'GTG Cuts at: 2128 2410 2128 Size: 282 5005 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2107 2139 3356 3388 4869 4901 2107 Size: 32 1217 32 1481 32 2493 Fragments arranged by size: 2493 1481 1217 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5287 PsiI TTA'TAA Cuts at: 2857 2857 Size: 5287 PspXI vC'TCGA_Gb Cuts at: 2041 2041 Size: 5287 PstI C_TGCA'G Cuts at: 830 2535 2639 830 Size: 1705 104 3478 Fragments arranged by size: 3478 1705 104 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5287 SacI G_AGCT'C Cuts at: 2478 2478 Size: 5287 SalI G'TCGA_C Cuts at: 1936 2629 1936 Size: 693 4594 SbfI CC_TGCA'GG Cuts at: 2639 2639 Size: 5287 ScaI AGT'ACT Cuts at: 3535 5247 3535 Size: 1712 3575 SexAI A'CCwGG_T Cuts at: 2355 2355 Size: 5287 SfcI C'TryA_G Cuts at: 826 1045 2531 2635 3770 4451 4642 826 Size: 219 1486 104 1135 681 191 1471 Fragments arranged by size: 1486 1471 1135 681 219 191 104 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5287 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5287 SmaI CCC'GGG Cuts at: 1872 2614 1872 Size: 742 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2041 3391 4292 4554 820 Size: 197 926 98 1350 901 262 1553 Fragments arranged by size: 1553 1350 926 901 262 197 98 SnaBI TAC'GTA Cuts at: 493 3078 493 Size: 2585 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5287 SphI G_CATG'C Cuts at: 1132 2645 1132 Size: 1513 3774 SspI AAT'ATT Cuts at: 5 52 3211 5 Size: 47 3159 2081 Fragments arranged by size: 3159 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5287 StyI C'CwwG_G Cuts at: 513 1065 2726 513 Size: 552 1661 3074 Fragments arranged by size: 3074 1661 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3314 3331 3484 3669 4114 325 Size: 1255 1734 17 153 185 445 1498 Fragments arranged by size: 1734 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2196 3533 5245 Size: 274 80 33 51 1443 219 1337 1712 Cuts at: 5245 96 Size: 138 Fragments arranged by size: 1712 1443 1337 274 219 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5247 5279 5247 Size: 32 5255 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5287 XbaI T'CTAG_A Cuts at: 2623 2623 Size: 5287 XhoI C'TCGA_G Cuts at: 1943 2041 1943 Size: 98 5189 XmaI C'CCGG_G Cuts at: 1870 2612 1870 Size: 742 4545 XmnI GAAnn'nnTTC Cuts at: 3416 3416 Size: 5287 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3095 276 Size: 53 83 186 936 1561 2468 Fragments arranged by size: 2468 1561 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PspXI PstI PvuI SacI SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PpuMI PspOMI PsrI PvuII RsrII SacII SanDI SapI SfiI SfoI SrfI SwaI XcmI