################################## # NO CUT ENZYMES ( 162 enzymes) # ################################## Acc65I AccI AccIII AclI AcsI AflII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp718I AspEI AvaI AviII AvrII BalI BanII BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BcnI BfaI BfrI BglI BglII BlnI BlpI BpmI Bpu1102I BpuAI BsaAI BsaBI BsaI BsaWI BseAI BseRI BsiEI BsiWI BsmBI BsmI BsoBI BspDI BspEI BspHI BspMI BsrBI BsrDI BssHII BssKI BssSI Bst1107I BstAPI BstBI BstNI BstXI BstZ17I Bsu36I CelII ClaI CpoI DraI DraIII DrdI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoRI EcoRII EcoRV EcoT22I FbaI FseI FspI HgaI HincII HindII HindIII HinfI HpaI KasI KpnI MaeI MamI MfeI MluI MluNI MroI MscI MseI MslI MunI MvaI MwoI NarI NciI NcoI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PflMI PinAI PleI PmaCI PmeI PmlI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SalI SapI SbfI ScaI ScrFI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspI StuI SwaI TfiI Tru9I Tsp509I Van91I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 44 enzymes) # ################################### AatII 355 GACGTC GACGT^C AccII 101 CGCG CG^CG AcyI 355 GACGTC G[GA]^CG[CT]C AflIII 323 ACATGT A^C[GA][CT]GT AluI 95 AGCT AG^CT Asp700I 456 GAAGCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspHI 424 GTGCTC G[AT]GC[AT]^C AspI 370 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC BamHI 212 GGATCC G^GATCC BsaHI 355 GACGTC G[GA]^CG[CT]C BsgI 277 CTGCAC GTGCAG(16/14) BsiHKAI 424 GTGCTC G[AT]GC[AT]^C BsmAI 269 GAGAC GTCTC(1/5) Bsp1407I 330 TGTACA T^GTACA BspLU11I 323 ACATGT A^CATGT BsrGI 330 TGTACA T^GTACA BstUI 101 CGCG CG^CG BstYI 212 GGATCC [GA]^GATC[CT] DraII 65 GGGTCCT [GA]G^G[ACGT]CC[CT] DsaI 100 CCGCGG C^C[GA][CT]GG EarI 60 GAAGAG CTCTTC(1/4) EcoNI 344 CCTGCTTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO109I 65 GGGTCCT [GA]G^G[ACGT]CC[CT] FauI 99 CCCGC CCCGC(4/6) HaeII 311 AGCGCC [GA]GCGC^[CT] HaeIII 395 GGCC GG^CC Hin1I 355 GACGTC G[GA]^CG[CT]C Ksp632I 60 GAAGAG CTCTTC(1/4) KspI 100 CCGCGG CCGC^GG MflI 212 GGATCC [GA]^GATC[CT] MspA1I 100 CCGCGG C[AC]G^C[GT]G MvnI 101 CGCG CG^CG NlaIII 324 CATG CATG^ NspI 323 ACATGT [GA]CATG^[CT] PflFI 370 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC PpuMI 65 GGGTCCT [GA]G^G[AT]CC[CT] PshAI 252 GACCATTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SacII 100 CCGCGG CCGC^GG SfaNI 243 GATGC GCATC(5/9) SmlI 427 CTCAAG C^T[CT][GA]AG SspBI 330 TGTACA T^GTACA Tth111I 370 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC XhoII 212 GGATCC [GA]^GATC[CT] XmnI 456 GAAGCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 39 enzymes) # ################################### AlwI 213 GATCC GGATC(4/5) 212 GGATC AvaII 66 GGTCC G^G[AT]CC 251 GGACC BanI 157 GGCACC G^G[CT][GA]CC 448 GGCACC BbvI 36 GCAGC GCAGC(8/12) 442 GCTGC BmyI 156 GGGCAC G[AGT]GC[ACT]^C 424 GTGCTC BsmFI 353 GGGAC GGGAC(10/14) 339 GTCCC Bsp1286I 156 GGGCAC G[AGT]GC[ACT]^C 424 GTGCTC BsrFI 392 GCCGGC [GA]^CCGG[CT] 445 GCCGGC BsrI 82 ACTGG ACTGG(1/-1) 187 ACTGG BstEII 228 GGTGACC G^GT[ACGT]ACC 432 GGTCACC BstF5I 261 CATCC GGATG(2/0) 285 CATCC BstPI 228 GGTGACC G^GT[ACGT]ACC 432 GGTCACC CfoI 312 GCGC GCG^C 414 GCGC Cfr10I 392 GCCGGC [GA]^CCGG[CT] 445 GCCGGC Cfr13I 66 GGTCC G^G[ACGT]CC 251 GGACC DdeI 17 CTGAG C^T[ACGT]AG 400 CTGAG DpnII 213 GATC ^GATC 363 GATC EcoO65I 228 GGTGACC G^GT[ACGT]ACC 432 GGTCACC EcoT14I 140 CCAAGG C^C[AT][AT]GG 437 CCAAGG Fnu4HI 36 GCAGC GC^[ACGT]GC 442 GCTGC FokI 261 CATCC GGATG(9/13) 285 CATCC HapII 393 CCGG C^CGG 446 CCGG HhaI 312 GCGC GCG^C 414 GCGC HinP1I 312 GCGC G^CGC 414 GCGC HpaII 393 CCGG C^CGG 446 CCGG ItaI 36 GCAGC GC^[ACGT]GC 442 GCTGC MaeII 356 ACGT A^CGT 422 ACGT MboI 213 GATC ^GATC 363 GATC MboII 60 GAAGA GAAGA(8/7) 240 GAAGA MspI 393 CCGG C^CGG 446 CCGG NaeI 392 GCCGGC GCC^GGC 445 GCCGGC NdeII 213 GATC ^GATC 363 GATC NgoMIV 392 GCCGGC G^CCGGC 445 GCCGGC Sau3AI 213 GATC ^GATC 363 GATC Sau96I 66 GGTCC G^G[ACGT]CC 251 GGACC StyI 140 CCAAGG C^C[AT][AT]GG 437 CCAAGG TaqI 204 TCGA T^CGA 305 TCGA TseI 36 GCAGC G^C[AT]GC 442 GCTGC TthHB8I 204 TCGA T^CGA 305 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 13 enzymes) # ######################################### AciI AfaI BsaJI BsiYI BslI Cac8I HphI MaeIII MnlI NlaIV RsaI Tsp45I TspRI