(Linear) (Six-base) MAPSORT of: KIEE5113.seq Check: 7646 from: 1 to: 474 KIEE5113.orf With 182 enzymes: SgfI * January 29, 2014 11:46 .. AatII G_ACGT'C Cuts at: 0 359 474 Size: 359 115 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 333 474 Size: 333 141 AflIII A'CryG_T Cuts at: 0 323 474 Size: 323 151 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 427 474 Size: 427 47 BamHI G'GATC_C Cuts at: 0 212 474 Size: 212 262 BanI G'GyrC_C Cuts at: 0 157 448 474 Size: 157 291 26 Fragments arranged by size: 291 157 26 Bme1580I G_kGCm'C Cuts at: 0 160 474 Size: 160 314 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 412 474 Size: 412 62 BsaHI Gr'CG_yC Cuts at: 0 356 474 Size: 356 118 BseYI C'CCAG_C Cuts at: 0 72 474 Size: 72 402 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 262 474 Size: 262 212 BsiHKAI G_wGCw'C Cuts at: 0 428 474 Size: 428 46 Bsp1286I G_dGCh'C Cuts at: 0 160 428 474 Size: 160 268 46 Fragments arranged by size: 268 160 46 BsrFI r'CCGG_y Cuts at: 0 392 445 474 Size: 392 53 29 Fragments arranged by size: 392 53 29 BsrGI T'GTAC_A Cuts at: 0 330 474 Size: 330 144 BstEII G'GTnAC_C Cuts at: 0 228 432 474 Size: 228 204 42 Fragments arranged by size: 228 204 42 BstYI r'GATC_y Cuts at: 0 212 474 Size: 212 262 BtgI C'CryG_G Cuts at: 0 100 474 Size: 100 374 EarI CTCTTCn'nnn_ Cuts at: 0 55 474 Size: 55 419 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 19 225 253 474 Size: 19 206 28 221 Fragments arranged by size: 221 206 28 19 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 333 474 Size: 333 141 EcoNI CCTnn'n_nnAGG Cuts at: 0 348 474 Size: 348 126 EcoO109I rG'GnC_Cy Cuts at: 0 66 474 Size: 66 408 HaeII r_GCGC'y Cuts at: 0 315 474 Size: 315 159 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 197 229 474 Size: 197 32 245 Fragments arranged by size: 245 197 32 MspA1I CmG'CkG Cuts at: 0 102 474 Size: 102 372 NaeI GCC'GGC Cuts at: 0 394 447 474 Size: 394 53 27 Fragments arranged by size: 394 53 27 NgoMIV G'CCGG_C Cuts at: 0 392 445 474 Size: 392 53 29 Fragments arranged by size: 392 53 29 NspI r_CATG'y Cuts at: 0 327 474 Size: 327 147 PciI A'CATG_T Cuts at: 0 323 474 Size: 323 151 PpuMI rG'GwC_Cy Cuts at: 0 66 474 Size: 66 408 PshAI GACnn'nnGTC Cuts at: 0 256 474 Size: 256 218 SacII CC_GC'GG Cuts at: 0 103 474 Size: 103 371 SmlI C'TyrA_G Cuts at: 0 427 474 Size: 427 47 StyI C'CwwG_G Cuts at: 0 140 437 474 Size: 140 297 37 Fragments arranged by size: 297 140 37 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 109 153 474 Size: 109 44 321 Fragments arranged by size: 321 109 44 TatI w'GTAC_w Cuts at: 0 293 330 474 Size: 293 37 144 Fragments arranged by size: 293 144 37 Tth111I GACn'n_nGTC Cuts at: 0 373 474 Size: 373 101 XmnI GAAnn'nnTTC Cuts at: 0 460 474 Size: 460 14 ZraI GAC'GTC Cuts at: 0 357 474 Size: 357 117 Enzymes that do cut: AatII AcuI AflIII AlfI BamHI BanI Bme1580I BpuEI BsaHI BseYI BsgI BsiHKAI Bsp1286I BsrFI BsrGI BstEII BstYI BtgI EarI EciI Eco57MI EcoNI EcoO109I HaeII Hin4I MspA1I NaeI NgoMIV NspI PciI PpuMI PshAI SacII SmlI StyI TaqII TatI Tth111I XmnI ZraI Enzymes that do not cut: AarI AccI Acc65I AclI AfeI AflII AgeI AhdI AleI AloI AlwNI ApaI ApaLI ApoI AscI AseI AsiSI AvaI AvrII BaeI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI BmgBI BmrI BmtI BplI BpmI Bpu10I BsaI BsaAI BsaBI BsaWI BsaXI BseRI BsiEI BsiWI BsmI BsmBI BspEI BspHI BspMI BsrBI BsrDI BssHII BssSI BstAPI BstBI BstXI BstZ17I Bsu36I BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EcoICRI EcoRI EcoRV FalI FseI FspI FspAI HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI NarI NcoI NdeI NheI NotI NruI NsiI PacI PasI PflMI PfoI PmeI PmlI PpiI PsiI PspOMI PspXI PsrI PstI PvuI PvuII RsrII SacI SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI StuI SwaI TstI XbaI XcmI XhoI XmaI