Restriction map for
pFN21AB5142
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# NO CUT ENZYMES ( 147 enzymes) #
##################################
AatII AccI AccII AccIII AclI
AflII AgeI AhdI Alw44I AlwNI
AocI Aor51HI ApaLI AscI Asp700I
AspEI AspI AvaI AvaII AviII
AvrII BalI BamHI BbrPI BbsI
BbvCI BclI BcnI BfaI BfrI
BglI BglII BlnI BpmI BpuAI
BsaAI BsaBI BsaI BsaWI BseAI
BsiEI BsiWI BsiYI BslI BsmBI
BsmFI BsmI BsoBI Bsp1407I BspEI
BspMI BsrBI BsrFI BsrGI BssHII
BssKI BssSI Bst1107I BstAPI BstEII
BstNI BstPI BstUI BstXI BstZ17I
Bsu36I Cfr10I CpoI DraI EaeI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO65I EcoRI EcoRII
EcoRV FauI FbaI FseI FspI
HapII HindIII HpaI HpaII HphI
KspI MaeI MamI MluI MluNI
MroI MscI MspI MvaI MvnI
NaeI NciI NdeI NgoMIV NheI
NotI NruI PacI PaeR7I PflFI
PflMI PinAI PleI PmaCI PmeI
PmlI PpuMI PshAI Psp1406I PstI
PvuI RsrII SacI SacII SalI
SapI SbfI ScaI ScrFI SexAI
SfcI SfiI SgrAI SmaI SmlI
SnaBI SpeI Sse8387I SspBI SwaI
Tth111I Van91I XbaI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 57 enzymes) #
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Acc65I 935 GGTACC G^GTACC
AciI 18 CCGC CCGC(-3/-1)
AcsI 283 AAATTT [GA]^AATT[CT]
AflIII 260 ACATGT A^C[GA][CT]GT
AlwI 232 GGATC GGATC(4/5)
ApaI 825 GGGCCC GGGCC^C
ApoI 283 AAATTT [GA]^AATT[CT]
AseI 562 ATTAAT AT^TAAT
AsnI 562 ATTAAT AT^TAAT
Asp718I 935 GGTACC G^GTACC
AspHI 185 GAGCAC G[AT]GC[AT]^C
BanII 825 GGGCCC G[GA]GC[CT]^C
BbeI 890 GGCGCC GGCGC^C
BlpI 979 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 979 GCTCAGC GC^T[ACGT]AGC
BsgI 411 CTGCAC GTGCAG(16/14)
BsiHKAI 185 GAGCAC G[AT]GC[AT]^C
BspDI 1276 ATCGAT AT^CGAT
BspHI 237 TCATGA T^CATGA
BspLU11I 260 ACATGT A^CATGT
BstBI 1215 TTCGAA TT^CGAA
BstYI 232 GGATCT [GA]^GATC[CT]
Cac8I 386 GCATGC GC[ACGT]^[ACGT]GC
CelII 979 GCTCAGC GC^T[ACGT]AGC
ClaI 1276 ATCGAT AT^CGAT
DpnII 233 GATC ^GATC
DraII 825 GGGCCCT [GA]G^G[ACGT]CC[CT]
DraIII 680 CACTCAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1245 GACTGTAAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 821 CCATGG C^C[GA][CT]GG
EcoO109I 825 GGGCCCT [GA]G^G[ACGT]CC[CT]
EcoT22I 970 ATGCAT ATGCA^T
HgaI 639 GCGTC GACGC(5/10)
KasI 890 GGCGCC G^GCGCC
KpnI 935 GGTACC GGTAC^C
MboI 233 GATC ^GATC
MfeI 704 CAATTG C^AATTG
MflI 232 GGATCT [GA]^GATC[CT]
MslI 1200 CATTGAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 1056 CAGCTG C[AC]G^C[GT]G
MunI 704 CAATTG C^AATTG
NarI 890 GGCGCC GG^CGCC
NcoI 821 CCATGG C^CATGG
NdeII 233 GATC ^GATC
NsiI 970 ATGCAT ATGCA^T
NspV 1215 TTCGAA TT^CGAA
PshBI 562 ATTAAT AT^TAAT
PvuII 1056 CAGCTG CAG^CTG
RcaI 237 TCATGA T^CATGA
Sau3AI 233 GATC ^GATC
SfoI 890 GGCGCC GGC^GCC
SfuI 1215 TTCGAA TT^CGAA
SphI 386 GCATGC GCATG^C
SspI 164 AATATT AAT^ATT
StuI 1070 AGGCCT AGG^CCT
XcmI 1129 CCATTTATAAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 232 GGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AcyI 638 GGCGTC G[GA]^CG[CT]C
890 GGCGCC
BanI 890 GGCGCC G^G[CT][GA]CC
935 GGTACC
BcgI 245 CGAATAAGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1278 CGATATTGATGC
BciVI 153 GTATCC GTATCC(6/5)
517 GTATCC
BmyI 185 GAGCAC G[AGT]GC[ACT]^C
825 GGGCCC
BsaHI 638 GGCGTC G[GA]^CG[CT]C
890 GGCGCC
BsaJI 68 CCAAGG C^C[ACGT][ACGT]GG
821 CCATGG
Bsp1286I 185 GAGCAC G[AGT]GC[ACT]^C
825 GGGCCC
BsrDI 86 GCAATG GCAATG(2/0)
967 GCAATG
CfoI 133 GCGC GCG^C
891 GCGC
Cfr13I 825 GGGCC G^G[ACGT]CC
826 GGCCC
EcoT14I 68 CCAAGG C^C[AT][AT]GG
821 CCATGG
HaeII 132 GGCGCT [GA]GCGC^[CT]
890 GGCGCC
HhaI 133 GCGC GCG^C
891 GCGC
Hin1I 638 GGCGTC G[GA]^CG[CT]C
890 GGCGCC
HinP1I 133 GCGC G^CGC
891 GCGC
MaeII 122 ACGT A^CGT
151 ACGT
MwoI 135 GCTTAATCGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
377 GCTATCGAGGC
Sau96I 825 GGGCC G^G[ACGT]CC
826 GGCCC
StyI 68 CCAAGG C^C[AT][AT]GG
821 CCATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 34 enzymes) #
#########################################
AfaI AluI BbvI BseRI BsmAI
BsrI BstF5I DdeI EarI Fnu4HI
FokI HaeIII HincII HindII HinfI
ItaI Ksp632I MaeIII MboII MnlI
MseI NlaIII NlaIV NspI RsaI
SfaNI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I