Restriction map for pFN21AB5142
 (Circular) (Six-base) MAPSORT of: pFN21AB5142.seq  Check: 4241  from: 1  to: 5998



pFN21AB5142  5998 bp

 With 182 enzymes: SgfI * 

                        October 20, 2009 11:00  ..

AatII G_ACGT'C

Cuts at:    278     331     414     600    1536    3808     278 
   Size:         53      83     186     936    2272    2468

  Fragments arranged by size: 

                2468    2272     936     186      83      53
 
AccI GT'mk_AC

Cuts at:   1937    3341    1937 
   Size:       1404    4594
 
Acc65I G'GTAC_C

Cuts at:   2940    3319    2940 
   Size:        379    5619
 
AclI AA'CG_TT

Cuts at:   1563    4125    4498    1563 
   Size:       2562     373    3063

  Fragments arranged by size: 

                3063    2562     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1632    2820    2868    4061    5445    5713    1632 
   Size:       1188      48    1193    1384     268    1917

  Fragments arranged by size: 

                1917    1384    1193    1188     268      48
 
AflII C'TTAA_G

Cuts at:    820    1017     820 
   Size:        197    5801
 
AflIII A'CryG_T

Cuts at:   1203    2265    4897    5874    1203 
   Size:       1062    2632     977    1327

  Fragments arranged by size: 

                2632    1327    1062     977
 
AgeI A'CCGG_T

Cuts at:   4829    4829 
   Size:       5998
 
AhdI GACnn_n'nnGTC

Cuts at:   4727    4727 
   Size:       5998
 
AleI CACnn'nnGTG

Cuts at:   1166    1166 
   Size:       5998
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1184    1184 
   Size:       5998
 
AlwNI CAG_nnn'CTG

Cuts at:   3741    5313    3741 
   Size:       1572    4426
 
ApaI G_GGCC'C

Cuts at:   2834    2834 
   Size:       5998
 
ApaLI G'TGCA_C

Cuts at:   4055    5211    4055 
   Size:       1156    4842
 
ApoI r'AATT_y

Cuts at:   1493    2288    3307    3533    3685    5794    1493 
   Size:        795    1019     226     152    2109    1697

  Fragments arranged by size: 

                2109    1697    1019     795     226     152
 
AseI AT'TA_AT

Cuts at:    160    1032    2568    4552     160 
   Size:        872    1536    1984    1606

  Fragments arranged by size: 

                1984    1606    1536     872
 
AsiSI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5998
 
AvaI C'yCGr_G

Cuts at:   1870    1943    3323    1870 
   Size:         73    1380    4545

  Fragments arranged by size: 

                4545    1380      73
 
BamHI G'GATC_C

Cuts at:   3328    3695    3328 
   Size:        367    5631
 
BanI G'GyrC_C

Cuts at:    618     943    1160    1787    2895    2940    3319    4774     618 
   Size:        325     217     627    1108      45     379    1455    1842

  Fragments arranged by size: 

                1842    1455    1108     627     379     325     217      45
            
 
BanII G_rGCy'C

Cuts at:   1391    2834    3317    1391 
   Size:       1443     483    4072

  Fragments arranged by size: 

                4072    1443     483
 
BbeI G_GCGC'C

Cuts at:   2899    2899 
   Size:       5998
 
BbsI GAAGACnn'nnnn_

Cuts at:    928    1899     928 
   Size:        971    5027
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1903    1937    2239    2273    3272    3306    4189    4223    1903 
   Size:         34     302      34     999      34     883      34    3678

  Fragments arranged by size: 

                3678     999     883     302      34      34      34      34
            
 
BciVI GTATCCnnnnn_n'

Cuts at:   2169    2533    3890    5100    2169 
   Size:        364    1357    1210    3067

  Fragments arranged by size: 

                3067    1357    1210     364
 
BclI T'GATC_A

Cuts at:   1548    1548 
   Size:       5998
 
BfrBI ATG'CAT

Cuts at:   2977    2977 
   Size:       5998
 
BglI GCCn_nnn'nGGC

Cuts at:    136     243     365     436    1067    1672    4609     136 
   Size:        107     122      71     631     605    2937    1525

  Fragments arranged by size: 

                2937    1525     631     605     122     107      71
 
BglII A'GATC_T

Cuts at:   5993    5993 
   Size:       5998
 
BlpI GC'TnA_GC

Cuts at:   2985    3415    2985 
   Size:        430    5568
 
Bme1580I G_kGCm'C

Cuts at:   1790    2834    4059    5215    1790 
   Size:       1044    1225    1156    2573

  Fragments arranged by size: 

                2573    1225    1156    1044
 
BmgBI CAC'GTC

Cuts at:   1318    1318 
   Size:       5998
 
BmrI ACTGGGnnnn_n'

Cuts at:    442     858     911     994    1393    1417    2194    2620    3729 
   Size:        416      53      83     399      24     777     426    1109

Cuts at:   3729    4687     442 
   Size:        958    1753

  Fragments arranged by size: 

                1753    1109     958     777     426     416     399      83
                  53      24
 
BmtI G_CTAG'C

Cuts at:   1056    1056 
   Size:       5998
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    4658    1239 
   Size:       3419    2579
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   4123    4988    5286    4123 
   Size:        865     298    4835

  Fragments arranged by size: 

                4835     865     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    882    1499    4661     882 
   Size:        617    3162    2219

  Fragments arranged by size: 

                3162    2219     617
 
BsaAI yAC'GTr

Cuts at:    493    1204    3789     493 
   Size:        711    2585    2702

  Fragments arranged by size: 

                2702    2585     711
 
BsaBI GATnn'nnATC

Cuts at:   5992    5992 
   Size:       5998
 
BsaHI Gr'CG_yC

Cuts at:    275     328     411     597    1533    2644    2896    3805    4187 
   Size:         53      83     186     936    1111     252     909     382

Cuts at:   4187    4822     275 
   Size:        635    1451

  Fragments arranged by size: 

                1451    1111     936     909     635     382     252     186
                  83      53
 
BsaWI w'CCGG_w

Cuts at:   4431    4829    5103    5250    5661    4431 
   Size:        398     274     147     411    4768

  Fragments arranged by size: 

                4768     411     398     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1178    1208    1373    1403    1178 
   Size:         30     165      30    5773

  Fragments arranged by size: 

                5773     165      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1186    2565    2612    2729    1186 
   Size:       1379      47     117    4455

  Fragments arranged by size: 

                4455    1379     117      47
 
BseYI C'CCAG_C

Cuts at:   2185    5201    5697    2185 
   Size:       3016     496    2486

  Fragments arranged by size: 

                3016    2486     496
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1736    2401    1163 
   Size:        573     665    4760

  Fragments arranged by size: 

                4760     665     573
 
BsiEI CG_ry'CG

Cuts at:   1540    1815    2001    3448    4209    5237    1540 
   Size:        275     186    1447     761    1028    2301

  Fragments arranged by size: 

                2301    1447    1028     761     275     186
 
BsiHKAI G_wGCw'C

Cuts at:   2194    4059    4144    5215    2194 
   Size:       1865      85    1071    2977

  Fragments arranged by size: 

                2977    1865    1071      85
 
BsmI GAATG_Cn'

Cuts at:   3509    3602    3509 
   Size:         93    5905
 
Bsp1286I G_dGCh'C

Cuts at:   1391    1790    2194    2834    3317    4059    4144    5215    1391 
   Size:        399     404     640     483     742      85    1071    2174

  Fragments arranged by size: 

                2174    1071     742     640     483     404     399      85
            
 
BspEI T'CCGG_A

Cuts at:   5661    5661 
   Size:       5998
 
BspHI T'CATG_A

Cuts at:   2242    3887    5617    2242 
   Size:       1645    1730    2623

  Fragments arranged by size: 

                2623    1730    1645
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    844    3353     844 
   Size:       2509    3489
 
BsrBI CCG'CTC

Cuts at:   1394    1823    3885    1394 
   Size:        429    2062    3507

  Fragments arranged by size: 

                3507    2062     429
 
BsrDI GCAATG_nn'

Cuts at:     65    1248    2098    2979    4493    4667      65 
   Size:       1183     850     881    1514     174    1396

  Fragments arranged by size: 

                1514    1396    1183     881     850     174
 
BsrFI r'CCGG_y

Cuts at:   1700    1810    4642    4829    1700 
   Size:        110    2832     187    2869

  Fragments arranged by size: 

                2869    2832     187     110
 
BsrGI T'GTAC_A

Cuts at:     96      96 
   Size:       5998
 
BssHII G'CGCG_C

Cuts at:   1925    1925 
   Size:       5998
 
BssSI C'ACGA_G

Cuts at:   4058    5070    4058 
   Size:       1012    4986
 
BstAPI GCAn_nnn'nTGC

Cuts at:    794     794 
   Size:       5998
 
BstBI TT'CG_AA

Cuts at:   3221    4802    3221 
   Size:       1581    4417
 
BstEII G'GTnAC_C

Cuts at:   1184    1184 
   Size:       5998
 
BstYI r'GATC_y

Cuts at:   1972    2237    3328    3695    4080    4097    5549    5635    5647 
   Size:        265    1091     367     385      17    1452      86      12

Cuts at:   5647    5993    1972 
   Size:        346    1977

  Fragments arranged by size: 

                1977    1452    1091     385     367     346     265      86
                  17      12
 
BtgI C'CryG_G

Cuts at:    513    1065    2826     513 
   Size:        552    1761    3685

  Fragments arranged by size: 

                3685    1761     552
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    487    1170    1226     487 
   Size:        683      56    5259

  Fragments arranged by size: 

                5259     683      56
 
BtsI GCAGTG_nn'

Cuts at:   2536    3514    4308    4328    2536 
   Size:        978     794      20    4206

  Fragments arranged by size: 

                4206     978     794      20
 
ClaI AT'CG_AT

Cuts at:   1554    3282    1554 
   Size:       1728    4270
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    529     564    2994    3029     529 
   Size:         35    2430      35    3498

  Fragments arranged by size: 

                3498    2430      35      35
 
DraI TTT'AAA

Cuts at:   3302    3302 
   Size:       5998
 
DraIII CAC_nnn'GTG

Cuts at:   2690    2690 
   Size:       5998
 
DrdI GACnn_nn'nnGTC

Cuts at:    809    1316    3256    5005     809 
   Size:        507    1940    1749    1802

  Fragments arranged by size: 

                1940    1802    1749     507
 
EaeI y'GGCC_r

Cuts at:      8      62    1487    1537    1812    1830    3445    4334    5963 
   Size:         54    1425      50     275      18    1615     889    1629

Cuts at:   5963       8 
   Size:         43

  Fragments arranged by size: 

                1629    1615    1425     889     275      54      50      43
                  18
 
EagI C'GGCC_G

Cuts at:   1537    1812    3445    1537 
   Size:        275    1633    4090

  Fragments arranged by size: 

                4090    1633     275
 
EarI CTCTTCn'nnn_

Cuts at:   1353    2589    2754    3042    3928    1353 
   Size:       1236     165     288     886    3423

  Fragments arranged by size: 

                3423    1236     886     288     165
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    387    1451    1589    4585    4959    5105     387 
   Size:       1064     138    2996     374     146    1280

  Fragments arranged by size: 

                2996    1280    1064     374     146     138
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    1632    2820    2868    4061    4658    5445    5713    1239 
   Size:        393    1188      48    1193     597     787     268    1524

  Fragments arranged by size: 

                1524    1193    1188     787     597     393     268      48
            
 
EcoO109I rG'GnC_Cy

Cuts at:   2831    2831 
   Size:       5998
 
EcoRI G'AATT_C

Cuts at:   3307    3685    3307 
   Size:        378    5620
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   3005    3005 
   Size:       5998
 
FspI TGC'GCA

Cuts at:   4504    4504 
   Size:       5998
 
HaeII r_GCGC'y

Cuts at:   1678    2141    2899    5145    1678 
   Size:        463     758    2246    2531

  Fragments arranged by size: 

                2531    2246     758     463
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1373    1405    1540    1572    2047    2079    3152    3184    4639 
   Size:         32     135      32     475      32    1073      32    1455

Cuts at:   4639    4671    4713    4745    1373 
   Size:         32      42      32    2626

  Fragments arranged by size: 

                2626    1455    1073     475     135      42      32      32
                  32      32      32      32
 
HincII GTy'rAC

Cuts at:    669    1657    1938    2020    2116    2125    3342    3588     669 
   Size:        988     281      82      96       9    1217     246    3079

  Fragments arranged by size: 

                3079    1217     988     281     246      96      82       9
            
 
HindIII A'AGCT_T

Cuts at:    748     748 
   Size:       5998
 
HpaI GTT'AAC

Cuts at:   3588    3588 
   Size:       5998
 
KasI G'GCGC_C

Cuts at:   2895    2895 
   Size:       5998
 
KpnI G_GTAC'C

Cuts at:   2944    3323    2944 
   Size:        379    5619
 
MfeI C'AATT_G

Cuts at:   2709    3597    2709 
   Size:        888    5110
 
MluI A'CGCG_T

Cuts at:   5874    5874 
   Size:       5998
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1305    2177    2250    3048    5112    5296    1305 
   Size:        872      73     798    2064     184    2007

  Fragments arranged by size: 

                2064    2007     872     798     184      73
 
MscI TGG'CCA

Cuts at:     10      64    1489    1832      10 
   Size:         54    1425     343    4176

  Fragments arranged by size: 

                4176    1425     343      54
 
MslI CAynn'nnrTG

Cuts at:    518    1166    3209    3956    4315    4474     518 
   Size:        648    2043     747     359     159    2042

  Fragments arranged by size: 

                2043    2042     747     648     359     159
 
MspA1I CmG'CkG

Cuts at:   1606    3063    3414    4091    5239    5484    1606 
   Size:       1457     351     677    1148     245    2120

  Fragments arranged by size: 

                2120    1457    1148     677     351     245
 
NarI GG'CG_CC

Cuts at:   2896    2896 
   Size:       5998
 
NcoI C'CATG_G

Cuts at:    513    1065    2826     513 
   Size:        552    1761    3685

  Fragments arranged by size: 

                3685    1761     552
 
NdeI CA'TA_TG

Cuts at:    387     387 
   Size:       5998
 
NheI G'CTAG_C

Cuts at:   1052    1052 
   Size:       5998
 
NotI GC'GGCC_GC

Cuts at:   3445    3445 
   Size:       5998
 
NsiI A_TGCA'T

Cuts at:   2979    2979 
   Size:       5998
 
NspI r_CATG'y

Cuts at:   1132    1229    2269    2336    2395    3356    4901    1132 
   Size:         97    1040      67      59     961    1545    2229

  Fragments arranged by size: 

                2229    1545    1040     961      97      67      59
 
PasI CC'CwG_GG

Cuts at:   1348    1348 
   Size:       5998
 
PciI A'CATG_T

Cuts at:   2265    4897    2265 
   Size:       2632    3366
 
PflMI CCAn_nnn'nTGG

Cuts at:   1108    1108 
   Size:       5998
 
PmeI GTTT'AAAC

Cuts at:   3302    3302 
   Size:       5998
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   4067    4099    5580    5612    4067 
   Size:         32    1481      32    4453

  Fragments arranged by size: 

                4453    1481      32      32
 
PshAI GACnn'nnGTC

Cuts at:   1613    1613 
   Size:       5998
 
PsiI TTA'TAA

Cuts at:   3140    3568    3140 
   Size:        428    5570
 
PspOMI G'GGCC_C

Cuts at:   2830    2830 
   Size:       5998
 
PstI C_TGCA'G

Cuts at:    830    3350     830 
   Size:       2520    3478
 
PvuI CG_AT'CG

Cuts at:   2001    2001 
   Size:       5998
 
PvuII CAG'CTG

Cuts at:   3063    3063 
   Size:       5998
 
SalI G'TCGA_C

Cuts at:   1936    3340    1936 
   Size:       1404    4594
 
SbfI CC_TGCA'GG

Cuts at:   3350    3350 
   Size:       5998
 
ScaI AGT'ACT

Cuts at:   4246    5958    4246 
   Size:       1712    4286
 
SfcI C'TryA_G

Cuts at:    826    1045    3346    4481    5162    5353     826 
   Size:        219    2301    1135     681     191    1471

  Fragments arranged by size: 

                2301    1471    1135     681     219     191
 
SfoI GGC'GCC

Cuts at:   2897    2897 
   Size:       5998
 
SgfI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5998
 
SgrAI Cr'CCGG_yG

Cuts at:   1700    1700 
   Size:       5998
 
SmaI CCC'GGG

Cuts at:   1872    3325    1872 
   Size:       1453    4545
 
SmlI C'TyrA_G

Cuts at:    820    1017    1943    4102    5003    5265     820 
   Size:        197     926    2159     901     262    1553

  Fragments arranged by size: 

                2159    1553     926     901     262     197
 
SnaBI TAC'GTA

Cuts at:    493    3789     493 
   Size:       3296    2702
 
SpeI A'CTAG_T

Cuts at:    152     152 
   Size:       5998
 
SphI G_CATG'C

Cuts at:   1132    2395    3356    1132 
   Size:       1263     961    3774

  Fragments arranged by size: 

                3774    1263     961
 
SspI AAT'ATT

Cuts at:      5      52    2171    3922       5 
   Size:         47    2119    1751    2081

  Fragments arranged by size: 

                2119    2081    1751      47
 
StuI AGG'CCT

Cuts at:   1517    3077    1517 
   Size:       1560    4438
 
StyI C'CwwG_G

Cuts at:    513    1065    2073    2826    3437     513 
   Size:        552    1008     753     611    3074

  Fragments arranged by size: 

                3074    1008     753     611     552
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    325    1580    4025    4042    4195    4380    4825     325 
   Size:       1255    2445      17     153     185     445    1498

  Fragments arranged by size: 

                2445    1498    1255     445     185     153      17
 
TatI w'GTAC_w

Cuts at:     96     370     450     483     534    1977    4244    5956      96 
   Size:        274      80      33      51    1443    2267    1712     138

  Fragments arranged by size: 

                2267    1712    1443     274     138      80      51      33
            
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   5958    5990    5958 
   Size:         32    5966
 
Tth111I GACn'n_nGTC

Cuts at:   1316    1316 
   Size:       5998
 
XbaI T'CTAG_A

Cuts at:   3334    3334 
   Size:       5998
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   3141    3141 
   Size:       5998
 
XhoI C'TCGA_G

Cuts at:   1943    1943 
   Size:       5998
 
XmaI C'CCGG_G

Cuts at:   1870    3323    1870 
   Size:       1453    4545
 
XmnI GAAnn'nnTTC

Cuts at:   4127    4127 
   Size:       5998
 
ZraI GAC'GTC

Cuts at:    276     329     412     598    1534    3806     276 
   Size:         53      83     186     936    2272    2468

  Fragments arranged by size: 

                2468    2272     936     186      83      53
 

 Enzymes that do cut:

    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI    AlwNI     ApaI    ApaLI     ApoI     AseI
    AsiSI     AvaI    BamHI     BanI    BanII     BbeI     BbsI     BcgI
    BciVI     BclI    BfrBI     BglI    BglII     BlpI Bme1580I    BmgBI
     BmrI     BmtI     BpmI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI
    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI
 Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI
   BssHII    BssSI   BstAPI    BstBI   BstEII    BstYI     BtgI    BtgZI
     BtsI     ClaI    CspCI     DraI   DraIII     DrdI     EaeI     EagI
     EarI     EciI  Eco57MI EcoO109I    EcoRI     FalI     FspI    HaeII
    Hin4I   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI
     MmeI     MscI     MslI   MspA1I     NarI     NcoI     NdeI     NheI
     NotI     NsiI     NspI     PasI     PciI    PflMI     PmeI     PpiI
    PshAI     PsiI   PspOMI     PstI     PvuI    PvuII     SalI     SbfI
     ScaI     SfcI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI
     SpeI     SphI     SspI     StuI     StyI    TaqII     TatI     TstI
  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AarI     AfeI     AloI     AscI    AvrII     BaeI    BbvCI     BplI
   Bpu10I    BsiWI    BsmBI    BstXI  BstZ17I   Bsu36I  EcoICRI    EcoNI
    EcoRV     FseI    FspAI     NaeI   NgoMIV     NruI     PacI     PfoI
     PmlI    PpuMI    PspXI     PsrI    RsrII     SacI    SacII    SanDI
     SapI    SexAI     SfiI     SrfI     SwaI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com