Restriction map for
pFN21AB5142
(Linear) (Six-base) MAPSORT of: KIBB5142.seq Check: 4048 from: 1 to: 1293
KIBB5142.orf
With 182 enzymes: SgfI *
April 24, 2009 13:04 ..
Acc65I G'GTAC_C
Cuts at: 0 935 1293
Size: 935 358
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 815 863 1293
Size: 815 48 430
Fragments arranged by size:
815 430 48
AflIII A'CryG_T
Cuts at: 0 260 1293
Size: 260 1033
ApaI G_GGCC'C
Cuts at: 0 829 1293
Size: 829 464
ApoI r'AATT_y
Cuts at: 0 283 1293
Size: 283 1010
AseI AT'TA_AT
Cuts at: 0 563 1293
Size: 563 730
BanI G'GyrC_C
Cuts at: 0 890 935 1293
Size: 890 45 358
Fragments arranged by size:
890 358 45
BanII G_rGCy'C
Cuts at: 0 829 1293
Size: 829 464
BbeI G_GCGC'C
Cuts at: 0 894 1293
Size: 894 399
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 234 268 1267 1293
Size: 234 34 999 26
Fragments arranged by size:
999 234 34 26
BciVI GTATCCnnnnn_n'
Cuts at: 0 164 528 1293
Size: 164 364 765
Fragments arranged by size:
765 364 164
BfrBI ATG'CAT
Cuts at: 0 972 1293
Size: 972 321
BlpI GC'TnA_GC
Cuts at: 0 980 1293
Size: 980 313
Bme1580I G_kGCm'C
Cuts at: 0 829 1293
Size: 829 464
BmrI ACTGGGnnnn_n'
Cuts at: 0 189 615 1293
Size: 189 426 678
Fragments arranged by size:
678 426 189
BsaHI Gr'CG_yC
Cuts at: 0 639 891 1293
Size: 639 252 402
Fragments arranged by size:
639 402 252
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 560 607 724 1293
Size: 560 47 117 569
Fragments arranged by size:
569 560 117 47
BseYI C'CCAG_C
Cuts at: 0 180 1293
Size: 180 1113
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 396 1293
Size: 396 897
BsiHKAI G_wGCw'C
Cuts at: 0 189 1293
Size: 189 1104
Bsp1286I G_dGCh'C
Cuts at: 0 189 829 1293
Size: 189 640 464
Fragments arranged by size:
640 464 189
BspHI T'CATG_A
Cuts at: 0 237 1293
Size: 237 1056
BsrDI GCAATG_nn'
Cuts at: 0 93 974 1293
Size: 93 881 319
Fragments arranged by size:
881 319 93
BstBI TT'CG_AA
Cuts at: 0 1216 1293
Size: 1216 77
BstYI r'GATC_y
Cuts at: 0 232 1293
Size: 232 1061
BtgI C'CryG_G
Cuts at: 0 821 1293
Size: 821 472
BtsI GCAGTG_nn'
Cuts at: 0 531 1293
Size: 531 762
ClaI AT'CG_AT
Cuts at: 0 1277 1293
Size: 1277 16
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 989 1024 1293
Size: 989 35 269
Fragments arranged by size:
989 269 35
DraIII CAC_nnn'GTG
Cuts at: 0 685 1293
Size: 685 608
DrdI GACnn_nn'nnGTC
Cuts at: 0 1251 1293
Size: 1251 42
EarI CTCTTCn'nnn_
Cuts at: 0 584 749 1037 1293
Size: 584 165 288 256
Fragments arranged by size:
584 288 256 165
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 815 863 1293
Size: 815 48 430
Fragments arranged by size:
815 430 48
EcoO109I rG'GnC_Cy
Cuts at: 0 826 1293
Size: 826 467
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1000 1293
Size: 1000 293
HaeII r_GCGC'y
Cuts at: 0 136 894 1293
Size: 136 758 399
Fragments arranged by size:
758 399 136
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 42 74 1147 1179 1293
Size: 42 32 1073 32 114
Fragments arranged by size:
1073 114 42 32 32
HincII GTy'rAC
Cuts at: 0 15 111 120 1293
Size: 15 96 9 1173
Fragments arranged by size:
1173 96 15 9
KasI G'GCGC_C
Cuts at: 0 890 1293
Size: 890 403
KpnI G_GTAC'C
Cuts at: 0 939 1293
Size: 939 354
MfeI C'AATT_G
Cuts at: 0 704 1293
Size: 704 589
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 172 245 1043 1293
Size: 172 73 798 250
Fragments arranged by size:
798 250 172 73
MslI CAynn'nnrTG
Cuts at: 0 1204 1293
Size: 1204 89
MspA1I CmG'CkG
Cuts at: 0 1058 1293
Size: 1058 235
NarI GG'CG_CC
Cuts at: 0 891 1293
Size: 891 402
NcoI C'CATG_G
Cuts at: 0 821 1293
Size: 821 472
NsiI A_TGCA'T
Cuts at: 0 974 1293
Size: 974 319
NspI r_CATG'y
Cuts at: 0 264 331 390 1293
Size: 264 67 59 903
Fragments arranged by size:
903 264 67 59
PciI A'CATG_T
Cuts at: 0 260 1293
Size: 260 1033
PsiI TTA'TAA
Cuts at: 0 1135 1293
Size: 1135 158
PspOMI G'GGCC_C
Cuts at: 0 825 1293
Size: 825 468
PvuII CAG'CTG
Cuts at: 0 1058 1293
Size: 1058 235
SfoI GGC'GCC
Cuts at: 0 892 1293
Size: 892 401
SphI G_CATG'C
Cuts at: 0 390 1293
Size: 390 903
SspI AAT'ATT
Cuts at: 0 166 1293
Size: 166 1127
StuI AGG'CCT
Cuts at: 0 1072 1293
Size: 1072 221
StyI C'CwwG_G
Cuts at: 0 68 821 1293
Size: 68 753 472
Fragments arranged by size:
753 472 68
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1136 1293
Size: 1136 157
Enzymes that do cut:
Acc65I AcuI AflIII ApaI ApoI AseI BanI BanII
BbeI BcgI BciVI BfrBI BlpI Bme1580I BmrI BsaHI
BseRI BseYI BsgI BsiHKAI Bsp1286I BspHI BsrDI BstBI
BstYI BtgI BtsI ClaI CspCI DraIII DrdI EarI
Eco57MI EcoO109I FalI HaeII Hin4I HincII KasI KpnI
MfeI MmeI MslI MspA1I NarI NcoI NsiI NspI
PciI PsiI PspOMI PvuII SfoI SphI SspI StuI
StyI XcmI
Enzymes that do not cut:
AarI AatII AccI AclI AfeI AflII AgeI AhdI
AleI AlfI AloI AlwNI ApaLI AscI AsiSI AvaI
AvrII BaeI BamHI BbsI BbvCI BclI BglI BglII
BmgBI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaWI BsaXI BsiEI BsiWI BsmI BsmBI BspEI
BspMI BsrBI BsrFI BsrGI BssHII BssSI BstAPI BstEII
BstXI BstZ17I Bsu36I BtgZI DraI EaeI EagI EciI
EcoICRI EcoNI EcoRI EcoRV FseI FspI FspAI HindIII
HpaI MluI MscI NaeI NdeI NgoMIV NheI NotI
NruI PacI PasI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PspXI PsrI PstI PvuI RsrII SacI
SacII SalI SanDI SapI SbfI ScaI SexAI SfcI
SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SrfI
SwaI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI