(Linear) (Six-base) MAPSORT of: KIEE1492.seq Check: 6087 from: 1 to: 2538 KIEE1492.orf With 182 enzymes: SgfI * July 15, 2010 18:40 .. Acc65I G'GTAC_C Cuts at: 0 798 2538 Size: 798 1740 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 23 1361 1388 2538 Size: 23 1338 27 1150 Fragments arranged by size: 1338 1150 27 23 AflII C'TTAA_G Cuts at: 0 301 2538 Size: 301 2237 AflIII A'CryG_T Cuts at: 0 650 1078 2538 Size: 650 428 1460 Fragments arranged by size: 1460 650 428 AhdI GACnn_n'nnGTC Cuts at: 0 678 1986 2538 Size: 678 1308 552 Fragments arranged by size: 1308 678 552 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 729 1903 2354 2538 Size: 729 1174 451 184 Fragments arranged by size: 1174 729 451 184 AlwNI CAG_nnn'CTG Cuts at: 0 1033 1572 2538 Size: 1033 539 966 Fragments arranged by size: 1033 966 539 ApoI r'AATT_y Cuts at: 0 181 1444 1774 2041 2538 Size: 181 1263 330 267 497 Fragments arranged by size: 1263 497 330 267 181 AvaI C'yCGr_G Cuts at: 0 344 2538 Size: 344 2194 AvrII C'CTAG_G Cuts at: 0 1429 2538 Size: 1429 1109 BanI G'GyrC_C Cuts at: 0 798 2538 Size: 798 1740 BanII G_rGCy'C Cuts at: 0 1870 2381 2538 Size: 1870 511 157 Fragments arranged by size: 1870 511 157 BbsI GAAGACnn'nnnn_ Cuts at: 0 1327 2538 Size: 1327 1211 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 301 335 2538 Size: 301 34 2203 Fragments arranged by size: 2203 301 34 BciVI GTATCCnnnnn_n' Cuts at: 0 81 2538 Size: 81 2457 BclI T'GATC_A Cuts at: 0 585 1710 2538 Size: 585 1125 828 Fragments arranged by size: 1125 828 585 BfrBI ATG'CAT Cuts at: 0 2065 2538 Size: 2065 473 BglII A'GATC_T Cuts at: 0 268 2538 Size: 268 2270 BlpI GC'TnA_GC Cuts at: 0 2296 2538 Size: 2296 242 BmrI ACTGGGnnnn_n' Cuts at: 0 2440 2538 Size: 2440 98 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1773 1889 2538 Size: 1773 116 649 Fragments arranged by size: 1773 649 116 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1856 2456 2538 Size: 1856 600 82 Fragments arranged by size: 1856 600 82 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 766 2538 Size: 766 1772 BsaI GGTCTCn'nnnn_ Cuts at: 0 419 1622 2538 Size: 419 1203 916 Fragments arranged by size: 1203 916 419 BsaAI yAC'GTr Cuts at: 0 1669 2538 Size: 1669 869 BsaBI GATnn'nnATC Cuts at: 0 790 914 2538 Size: 790 124 1624 Fragments arranged by size: 1624 790 124 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 2140 2170 2538 Size: 2140 30 368 Fragments arranged by size: 2140 368 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 1984 2538 Size: 1984 554 BseYI C'CCAG_C Cuts at: 0 937 2538 Size: 937 1601 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 627 1286 1505 2538 Size: 627 659 219 1033 Fragments arranged by size: 1033 659 627 219 BsiHKAI G_wGCw'C Cuts at: 0 2086 2381 2538 Size: 2086 295 157 Fragments arranged by size: 2086 295 157 Bsp1286I G_dGCh'C Cuts at: 0 1870 2086 2381 2538 Size: 1870 216 295 157 Fragments arranged by size: 1870 295 216 157 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 63 1502 2286 2538 Size: 63 1439 784 252 Fragments arranged by size: 1439 784 252 63 BsrDI GCAATG_nn' Cuts at: 0 1885 2538 Size: 1885 653 BsrFI r'CCGG_y Cuts at: 0 2307 2538 Size: 2307 231 BssSI C'ACGA_G Cuts at: 0 433 2538 Size: 433 2105 BstXI CCAn_nnnn'nTGG Cuts at: 0 2035 2538 Size: 2035 503 BstYI r'GATC_y Cuts at: 0 268 516 2538 Size: 268 248 2022 Fragments arranged by size: 2022 268 248 BtgI C'CryG_G Cuts at: 0 383 2538 Size: 383 2155 BtsI GCAGTG_nn' Cuts at: 0 685 1031 2047 2538 Size: 685 346 1016 491 Fragments arranged by size: 1016 685 491 346 ClaI AT'CG_AT Cuts at: 0 1013 2538 Size: 1013 1525 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 1169 1204 1784 1819 2538 Size: 1169 35 580 35 719 Fragments arranged by size: 1169 719 580 35 35 DraI TTT'AAA Cuts at: 0 551 2400 2538 Size: 551 1849 138 Fragments arranged by size: 1849 551 138 DraIII CAC_nnn'GTG Cuts at: 0 691 2538 Size: 691 1847 EaeI y'GGCC_r Cuts at: 0 2511 2538 Size: 2511 27 EarI CTCTTCn'nnn_ Cuts at: 0 270 629 971 1742 2317 2477 2538 Size: 270 359 342 771 575 160 61 Fragments arranged by size: 771 575 359 342 270 160 61 EcoICRI GAG'CTC Cuts at: 0 2379 2538 Size: 2379 159 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 23 1361 1388 1856 2456 2538 Size: 23 1338 27 468 600 82 Fragments arranged by size: 1338 600 468 82 27 23 EcoO109I rG'GnC_Cy Cuts at: 0 2275 2538 Size: 2275 263 EcoRI G'AATT_C Cuts at: 0 2041 2538 Size: 2041 497 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 313 2538 Size: 313 2225 FspI TGC'GCA Cuts at: 0 458 2538 Size: 458 2080 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 664 696 764 796 1531 1563 1735 1767 Size: 664 32 68 32 735 32 172 32 Cuts at: 1767 1972 2004 2140 2172 2253 2285 2316 2348 Size: 205 32 136 32 81 32 31 32 Cuts at: 2348 2444 2476 2538 Size: 96 32 62 Fragments arranged by size: 735 664 205 172 136 96 81 68 62 32 32 32 32 32 32 32 32 32 31 HincII GTy'rAC Cuts at: 0 1653 2538 Size: 1653 885 HindIII A'AGCT_T Cuts at: 0 35 2071 2416 2538 Size: 35 2036 345 122 Fragments arranged by size: 2036 345 122 35 KpnI G_GTAC'C Cuts at: 0 802 2538 Size: 802 1736 MfeI C'AATT_G Cuts at: 0 1165 2538 Size: 1165 1373 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 2487 2538 Size: 2487 51 MslI CAynn'nnrTG Cuts at: 0 52 1036 2336 2538 Size: 52 984 1300 202 Fragments arranged by size: 1300 984 202 52 NaeI GCC'GGC Cuts at: 0 2309 2538 Size: 2309 229 NcoI C'CATG_G Cuts at: 0 383 2538 Size: 383 2155 NgoMIV G'CCGG_C Cuts at: 0 2307 2538 Size: 2307 231 NsiI A_TGCA'T Cuts at: 0 2067 2538 Size: 2067 471 NspI r_CATG'y Cuts at: 0 382 654 716 1082 2015 2538 Size: 382 272 62 366 933 523 Fragments arranged by size: 933 523 382 366 272 62 PciI A'CATG_T Cuts at: 0 650 1078 2538 Size: 650 428 1460 Fragments arranged by size: 1460 650 428 PflMI CCAn_nnn'nTGG Cuts at: 0 1572 2538 Size: 1572 966 PpuMI rG'GwC_Cy Cuts at: 0 2275 2538 Size: 2275 263 PshAI GACnn'nnGTC Cuts at: 0 1137 2538 Size: 1137 1401 PstI C_TGCA'G Cuts at: 0 2054 2241 2538 Size: 2054 187 297 Fragments arranged by size: 2054 297 187 SacI G_AGCT'C Cuts at: 0 2381 2538 Size: 2381 157 SapI GCTCTTCn'nnn_ Cuts at: 0 971 2538 Size: 971 1567 ScaI AGT'ACT Cuts at: 0 1185 1212 2538 Size: 1185 27 1326 Fragments arranged by size: 1326 1185 27 SexAI A'CCwGG_T Cuts at: 0 1385 2538 Size: 1385 1153 SfcI C'TryA_G Cuts at: 0 2050 2237 2538 Size: 2050 187 301 Fragments arranged by size: 2050 301 187 SmlI C'TyrA_G Cuts at: 0 301 344 781 2538 Size: 301 43 437 1757 Fragments arranged by size: 1757 437 301 43 SphI G_CATG'C Cuts at: 0 716 2538 Size: 716 1822 SspI AAT'ATT Cuts at: 0 2124 2531 2538 Size: 2124 407 7 Fragments arranged by size: 2124 407 7 StyI C'CwwG_G Cuts at: 0 383 1231 1429 2538 Size: 383 848 198 1109 Fragments arranged by size: 1109 848 383 198 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1812 2538 Size: 1812 726 TatI w'GTAC_w Cuts at: 0 238 1183 1210 2538 Size: 238 945 27 1328 Fragments arranged by size: 1328 945 238 27 XbaI T'CTAG_A Cuts at: 0 271 2538 Size: 271 2267 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 568 2538 Size: 568 1970 XhoI C'TCGA_G Cuts at: 0 344 2538 Size: 344 2194 XmnI GAAnn'nnTTC Cuts at: 0 2417 2538 Size: 2417 121 Enzymes that do cut: Acc65I AcuI AflII AflIII AhdI AlfI AlwNI ApoI AvaI AvrII BanI BanII BbsI BcgI BciVI BclI BfrBI BglII BlpI BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaXI BseRI BseYI BsgI BsiHKAI Bsp1286I BspMI BsrDI BsrFI BssSI BstXI BstYI BtgI BtsI ClaI CspCI DraI DraIII EaeI EarI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI Hin4I HincII HindIII KpnI MfeI MmeI MslI NaeI NcoI NgoMIV NsiI NspI PciI PflMI PpuMI PshAI PstI SacI SapI ScaI SexAI SfcI SmlI SphI SspI StyI TaqII TatI XbaI XcmI XhoI XmnI Enzymes that do not cut: AarI AatII AccI AclI AfeI AgeI AleI AloI ApaI ApaLI AscI AseI AsiSI BaeI BamHI BbeI BbvCI BglI Bme1580I BmgBI BmtI Bpu10I BsaHI BsaWI BsiEI BsiWI BsmI BsmBI BspEI BspHI BsrBI BsrGI BssHII BstAPI BstBI BstEII BstZ17I Bsu36I BtgZI DrdI EagI EciI EcoNI EcoRV FseI FspAI HaeII HpaI KasI MluI MscI MspA1I NarI NdeI NheI NotI NruI PacI PasI PfoI PmeI PmlI PpiI PsiI PspOMI PspXI PsrI PvuI PvuII RsrII SacII SalI SanDI SbfI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SrfI StuI SwaI TstI Tth111I XmaI ZraI