################################## # NO CUT ENZYMES ( 155 enzymes) # ################################## AatII AccIII AclI AcyI AflII AflIII AgeI AhdI Alw44I AlwI AlwNI AocI Aor51HI ApaLI AscI AseI AsnI Asp700I AspEI AspI AvaI AviII AvrII BalI BamHI BbeI BbsI BbvCI BcgI BciVI BclI BcnI BfrI BglI BlnI BlpI BpmI Bpu1102I BpuAI BsaHI BsaI BsaWI BseAI BsiEI BsiWI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BssHII Bst1107I BstAPI BstBI BstEII BstPI BstZ17I Bsu36I Cac8I CelII CfoI Cfr10I ClaI CpoI DraI DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FbaI FseI FspI HaeII HapII HhaI Hin1I HinP1I HincII HindII HindIII HpaI HpaII KasI MfeI MluI MluNI MroI MscI MspI MunI NaeI NarI NciI NcoI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PleI PmeI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SalI SbfI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI Tth111I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 34 enzymes) # ################################### Acc65I 1067 GGTACC G^GTACC AccI 275 GTAGAC GT^[AC][GT]AC AccII 1105 CGCG CG^CG AcsI 408 GAATTT [GA]^AATT[CT] ApaI 895 GGGCCC GGGCC^C ApoI 408 GAATTT [GA]^AATT[CT] Asp718I 1067 GGTACC G^GTACC BanI 1067 GGTACC G^G[CT][GA]CC BfaI 692 CTAG C^TAG BglII 490 AGATCT A^GATCT BsaBI 757 GATGACTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsgI 494 CTGCAC GTGCAG(16/14) BstUI 1105 CGCG CG^CG BstXI 233 CCATCAAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 490 AGATCT [GA]^GATC[CT] DraIII 872 CACGTAGTG CAC[ACGT][ACGT][ACGT]^GTG EaeI 1125 TGGCCG [CT]^GGCC[GA] FauI 900 CCCGC CCCGC(4/6) HgaI 181 GACGC GACGC(5/10) HphI 161 TCACC GGTGA(8/7) KpnI 1067 GGTACC GGTAC^C KspI 1104 CCGCGG CCGC^GG MaeI 692 CTAG C^TAG MamI 757 GATGACTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MflI 490 AGATCT [GA]^GATC[CT] MvnI 1105 CGCG CG^CG NdeI 851 CATATG CA^TATG SacI 539 GAGCTC GAGCT^C SacII 1104 CCGCGG CCGC^GG ScaI 108 AGTACT AGT^ACT SfaNI 190 GATGC GCATC(5/9) Tsp509I 409 AATT ^AATT TspRI 522 CAGTG CA[GC]TG[ACGT][ACGT]^ XhoII 490 AGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 17 enzymes) # ################################### AfaI 109 GTAC GT^AC 1068 GTAC BanII 539 GAGCTC G[GA]GC[CT]^C 895 GGGCCC BsmAI 200 GAGAC GTCTC(1/5) 971 GAGAC DsaI 1104 CCGCGG C^C[GA][CT]GG 1128 CCGTGG EcoNI 313 CCTCCAAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 886 CCTACACGAGG HinfI 847 GATTC G^A[ACGT]TC 1084 GATTC MseI 44 TTAA T^TAA 413 TTAA MspA1I 592 CCGCTG C[AC]G^C[GT]G 1104 CCGCGG PpuMI 360 GGGACCT [GA]G^G[AT]CC[CT] 474 GGGTCCT RsaI 109 GTAC GT^AC 1068 GTAC SapI 536 GAAGAGC GCTCTTC(1/4) 27 GCTCTTC SfcI 840 CTACAG C^T[GA][CT]AG 1089 CTACAG SmlI 981 CTCAAG C^T[CT][GA]AG 1005 CTCAAG TaqI 582 TCGA T^CGA 969 TCGA TfiI 847 GATTC G^A[AT]TC 1084 GATTC Tru9I 44 TTAA T^TAA 413 TTAA TthHB8I 582 TCGA T^CGA 969 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 52 enzymes) # ######################################### AciI AluI AspHI AvaII BbrPI BbvI BmyI BsaAI BsaJI BseRI BsiHKAI BsiYI BslI BsmFI Bsp1286I BsrI BssKI BssSI BstF5I BstNI Cfr13I DdeI DpnII DraII EarI EcoO109I EcoRII Fnu4HI FokI HaeIII ItaI Ksp632I MaeII MaeIII MboI MboII MnlI MslI MvaI MwoI NdeII NlaIII NlaIV PflMI PmaCI PmlI Sau3AI Sau96I ScrFI TseI Tsp45I Van91I