Restriction map for pFN21AE4373
 (Circular) (Six-base) MAPSORT of: pFN21AE4373.seq  Check: 9538  from: 1  to: 5878



pFN21AE4373  5878 bp

 With 182 enzymes: SgfI * 

                        March 28, 2012 13:33  ..

AatII G_ACGT'C

Cuts at:    278     331     414     600    1536    3688     278 
   Size:         53      83     186     936    2152    2468

  Fragments arranged by size: 

                2468    2152     936     186      83      53
 
AccI GT'mk_AC

Cuts at:   1937    2281    3221    1937 
   Size:        344     940    4594

  Fragments arranged by size: 

                4594     940     344
 
Acc65I G'GTAC_C

Cuts at:   3072    3199    3072 
   Size:        127    5751
 
AclI AA'CG_TT

Cuts at:   1563    4005    4378    1563 
   Size:       2442     373    3063

  Fragments arranged by size: 

                3063    2442     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1632    2490    3941    5325    5593    1632 
   Size:        858    1451    1384     268    1917

  Fragments arranged by size: 

                1917    1451    1384     858     268
 
AflII C'TTAA_G

Cuts at:    820    1017     820 
   Size:        197    5681
 
AflIII A'CryG_T

Cuts at:   1203    4777    5754    1203 
   Size:       3574     977    1327

  Fragments arranged by size: 

                3574    1327     977
 
AgeI A'CCGG_T

Cuts at:   4709    4709 
   Size:       5878
 
AhdI GACnn_n'nnGTC

Cuts at:   4607    4607 
   Size:       5878
 
AleI CACnn'nnGTG

Cuts at:   1166    1166 
   Size:       5878
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1184    1184 
   Size:       5878
 
AlwNI CAG_nnn'CTG

Cuts at:   3621    5193    3621 
   Size:       1572    4306
 
ApaI G_GGCC'C

Cuts at:   2904    2904 
   Size:       5878
 
ApaLI G'TGCA_C

Cuts at:   3935    5091    3935 
   Size:       1156    4722
 
ApoI r'AATT_y

Cuts at:   1493    2413    3187    3413    3565    5674    1493 
   Size:        920     774     226     152    2109    1697

  Fragments arranged by size: 

                2109    1697     920     774     226     152
 
AseI AT'TA_AT

Cuts at:    160    1032    4432     160 
   Size:        872    3400    1606

  Fragments arranged by size: 

                3400    1606     872
 
AsiSI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5878
 
AvaI C'yCGr_G

Cuts at:   1870    1943    3203    1870 
   Size:         73    1260    4545

  Fragments arranged by size: 

                4545    1260      73
 
BamHI G'GATC_C

Cuts at:   3208    3575    3208 
   Size:        367    5511
 
BanI G'GyrC_C

Cuts at:    618     943    1160    1787    3072    3199    4654     618 
   Size:        325     217     627    1285     127    1455    1842

  Fragments arranged by size: 

                1842    1455    1285     627     325     217     127
 
BanII G_rGCy'C

Cuts at:   1391    2548    2904    3197    1391 
   Size:       1157     356     293    4072

  Fragments arranged by size: 

                4072    1157     356     293
 
BbsI GAAGACnn'nnnn_

Cuts at:    928    1899     928 
   Size:        971    4907
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1903    1937    4069    4103    1903 
   Size:         34    2132      34    3678

  Fragments arranged by size: 

                3678    2132      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   3770    4980    3770 
   Size:       1210    4668
 
BclI T'GATC_A

Cuts at:   1548    1548 
   Size:       5878
 
BglI GCCn_nnn'nGGC

Cuts at:    136     243     365     436    1067    1672    4489     136 
   Size:        107     122      71     631     605    2817    1525

  Fragments arranged by size: 

                2817    1525     631     605     122     107      71
 
BglII A'GATC_T

Cuts at:   2495    5873    2495 
   Size:       3378    2500
 
BlpI GC'TnA_GC

Cuts at:   3295    3295 
   Size:       5878
 
Bme1580I G_kGCm'C

Cuts at:   1790    2904    3939    5095    1790 
   Size:       1114    1035    1156    2573

  Fragments arranged by size: 

                2573    1156    1114    1035
 
BmgBI CAC'GTC

Cuts at:   1318    2285    1318 
   Size:        967    4911
 
BmrI ACTGGGnnnn_n'

Cuts at:    442     858     911     994    1393    1417    2441    3609    4567 
   Size:        416      53      83     399      24    1024    1168     958

Cuts at:   4567     442 
   Size:       1753

  Fragments arranged by size: 

                1753    1168    1024     958     416     399      83      53
                  24
 
BmtI G_CTAG'C

Cuts at:   1056    1056 
   Size:       5878
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    4538    1239 
   Size:       3299    2579
 
Bpu10I CC'TnA_GC

Cuts at:   2190    2190 
   Size:       5878
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   2971    2995    4003    4868    5166    2971 
   Size:         24    1008     865     298    3683

  Fragments arranged by size: 

                3683    1008     865     298      24
 
BsaI GGTCTCn'nnnn_

Cuts at:    882    1499    4541     882 
   Size:        617    3042    2219

  Fragments arranged by size: 

                3042    2219     617
 
BsaAI yAC'GTr

Cuts at:    493    1204    2558    2759    2879    2954    3086    3669     493 
   Size:        711    1354     201     120      75     132     583    2702

  Fragments arranged by size: 

                2702    1354     711     583     201     132     120      75
            
 
BsaBI GATnn'nnATC

Cuts at:   2766    5872    2766 
   Size:       3106    2772
 
BsaHI Gr'CG_yC

Cuts at:    275     328     411     597    1533    3685    4067    4702     275 
   Size:         53      83     186     936    2152     382     635    1451

  Fragments arranged by size: 

                2152    1451     936     635     382     186      83      53
            
 
BsaWI w'CCGG_w

Cuts at:   4311    4709    4983    5130    5541    4311 
   Size:        398     274     147     411    4648

  Fragments arranged by size: 

                4648     411     398     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1178    1208    1373    1403    1178 
   Size:         30     165      30    5653

  Fragments arranged by size: 

                5653     165      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1186    2358    2475    3069    1186 
   Size:       1172     117     594    3995

  Fragments arranged by size: 

                3995    1172     594     117
 
BseYI C'CCAG_C

Cuts at:   2179    5081    5577    2179 
   Size:       2902     496    2480

  Fragments arranged by size: 

                2902    2480     496
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1736    2484    1163 
   Size:        573     748    4557

  Fragments arranged by size: 

                4557     748     573
 
BsiEI CG_ry'CG

Cuts at:   1540    1815    2001    3328    4089    5117    1540 
   Size:        275     186    1327     761    1028    2301

  Fragments arranged by size: 

                2301    1327    1028     761     275     186
 
BsiHKAI G_wGCw'C

Cuts at:   2548    2887    3118    3939    4024    5095    2548 
   Size:        339     231     821      85    1071    3331

  Fragments arranged by size: 

                3331    1071     821     339     231      85
 
BsmI GAATG_Cn'

Cuts at:   3389    3482    3389 
   Size:         93    5785
 
Bsp1286I G_dGCh'C

Cuts at:   1391    1790    2548    2887    2904    3118    3197    3939    4024 
   Size:        399     758     339      17     214      79     742      85

Cuts at:   4024    5095    1391 
   Size:       1071    2174

  Fragments arranged by size: 

                2174    1071     758     742     399     339     214      85
                  79      17
 
BspEI T'CCGG_A

Cuts at:   5541    5541 
   Size:       5878
 
BspHI T'CATG_A

Cuts at:   3767    5497    3767 
   Size:       1730    4148
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    844    3233     844 
   Size:       2389    3489
 
BsrBI CCG'CTC

Cuts at:   1394    1823    3765    1394 
   Size:        429    1942    3507

  Fragments arranged by size: 

                3507    1942     429
 
BsrDI GCAATG_nn'

Cuts at:     65    1248    4373    4547      65 
   Size:       1183    3125     174    1396

  Fragments arranged by size: 

                3125    1396    1183     174
 
BsrFI r'CCGG_y

Cuts at:   1700    1810    4522    4709    1700 
   Size:        110    2712     187    2869

  Fragments arranged by size: 

                2869    2712     187     110
 
BsrGI T'GTAC_A

Cuts at:     96      96 
   Size:       5878
 
BssHII G'CGCG_C

Cuts at:   1925    1925 
   Size:       5878
 
BssSI C'ACGA_G

Cuts at:   2688    2718    2895    3938    4950    2688 
   Size:         30     177    1043    1012    3616

  Fragments arranged by size: 

                3616    1043    1012     177      30
 
BstAPI GCAn_nnn'nTGC

Cuts at:    794     794 
   Size:       5878
 
BstBI TT'CG_AA

Cuts at:   4682    4682 
   Size:       5878
 
BstEII G'GTnAC_C

Cuts at:   1184    1184 
   Size:       5878
 
BstXI CCAn_nnnn'nTGG

Cuts at:   2245    2245 
   Size:       5878
 
BstYI r'GATC_y

Cuts at:   1972    2495    3208    3575    3960    3977    5429    5515    5527 
   Size:        523     713     367     385      17    1452      86      12

Cuts at:   5527    5873    1972 
   Size:        346    1977

  Fragments arranged by size: 

                1977    1452     713     523     385     367     346      86
                  17      12
 
BtgI C'CryG_G

Cuts at:    513    1065    2004    3109    3133     513 
   Size:        552     939    1105      24    3258

  Fragments arranged by size: 

                3258    1105     939     552      24
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    487    1170    1226     487 
   Size:        683      56    5139

  Fragments arranged by size: 

                5139     683      56
 
BtsI GCAGTG_nn'

Cuts at:   2533    3394    4188    4208    2533 
   Size:        861     794      20    4203

  Fragments arranged by size: 

                4203     861     794      20
 
ClaI AT'CG_AT

Cuts at:   1554    1554 
   Size:       5878
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    529     564     529 
   Size:         35    5843
 
DraI TTT'AAA

Cuts at:   3182    3182 
   Size:       5878
 
DraIII CAC_nnn'GTG

Cuts at:   2882    2882 
   Size:       5878
 
DrdI GACnn_nn'nnGTC

Cuts at:    809    1316    4885     809 
   Size:        507    3569    1802

  Fragments arranged by size: 

                3569    1802     507
 
EaeI y'GGCC_r

Cuts at:      8      62    1487    1537    1812    1830    3130    3325    4214 
   Size:         54    1425      50     275      18    1300     195     889

Cuts at:   4214    5843       8 
   Size:       1629      43

  Fragments arranged by size: 

                1629    1425    1300     889     275     195      54      50
                  43      18
 
EagI C'GGCC_G

Cuts at:   1537    1812    3325    1537 
   Size:        275    1513    4090

  Fragments arranged by size: 

                4090    1513     275
 
EarI CTCTTCn'nnn_

Cuts at:   1353    2039    2335    2536    2557    3808    1353 
   Size:        686     296     201      21    1251    3423

  Fragments arranged by size: 

                3423    1251     686     296     201      21
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    387    1451    1589    4465    4839    4985     387 
   Size:       1064     138    2876     374     146    1280

  Fragments arranged by size: 

                2876    1280    1064     374     146     138
 
EcoICRI GAG'CTC

Cuts at:   2546    2546 
   Size:       5878
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    1632    2490    3941    4538    5325    5593    1239 
   Size:        393     858    1451     597     787     268    1524

  Fragments arranged by size: 

                1524    1451     858     787     597     393     268
 
EcoNI CCTnn'n_nnAGG

Cuts at:   2322    2895    2322 
   Size:        573    5305
 
EcoO109I rG'GnC_Cy

Cuts at:   2315    2366    2480    2900    2901    2315 
   Size:         51     114     420       1    5292

  Fragments arranged by size: 

                5292     420     114      51       1
 
EcoRI G'AATT_C

Cuts at:   3187    3565    3187 
   Size:        378    5500
 
FspI TGC'GCA

Cuts at:   4384    4384 
   Size:       5878
 
HaeII r_GCGC'y

Cuts at:   1678    5025    1678 
   Size:       3347    2531
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1373    1405    1540    1572    2756    2788    2969    2993    3001 
   Size:         32     135      32    1184      32     181      24       8

Cuts at:   3001    3025    4519    4551    4593    4625    1373 
   Size:         24    1494      32      42      32    2626

  Fragments arranged by size: 

                2626    1494    1184     181     135      42      32      32
                  32      32      32      24      24       8
 
HincII GTy'rAC

Cuts at:    669    1657    1938    3222    3468     669 
   Size:        988     281    1284     246    3079

  Fragments arranged by size: 

                3079    1284     988     281     246
 
HindIII A'AGCT_T

Cuts at:    748     748 
   Size:       5878
 
HpaI GTT'AAC

Cuts at:   3468    3468 
   Size:       5878
 
KpnI G_GTAC'C

Cuts at:   3076    3203    3076 
   Size:        127    5751
 
MfeI C'AATT_G

Cuts at:   3477    3477 
   Size:       5878
 
MluI A'CGCG_T

Cuts at:   5754    5754 
   Size:       5878
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1305    4992    5176    1305 
   Size:       3687     184    2007

  Fragments arranged by size: 

                3687    2007     184
 
MscI TGG'CCA

Cuts at:     10      64    1489    1832      10 
   Size:         54    1425     343    4056

  Fragments arranged by size: 

                4056    1425     343      54
 
MslI CAynn'nnrTG

Cuts at:    518    1166    2243    2391    2832    3836    4195    4354     518 
   Size:        648    1077     148     441    1004     359     159    2042

  Fragments arranged by size: 

                2042    1077    1004     648     441     359     159     148
            
 
MspA1I CmG'CkG

Cuts at:   1606    2599    3111    3294    3971    5119    5364    1606 
   Size:        993     512     183     677    1148     245    2120

  Fragments arranged by size: 

                2120    1148     993     677     512     245     183
 
NcoI C'CATG_G

Cuts at:    513    1065    2004     513 
   Size:        552     939    4387

  Fragments arranged by size: 

                4387     939     552
 
NdeI CA'TA_TG

Cuts at:    387    2857     387 
   Size:       2470    3408
 
NheI G'CTAG_C

Cuts at:   1052    1052 
   Size:       5878
 
NotI GC'GGCC_GC

Cuts at:   3325    3325 
   Size:       5878
 
NspI r_CATG'y

Cuts at:   1132    1229    3236    4781    1132 
   Size:         97    2007    1545    2229

  Fragments arranged by size: 

                2229    2007    1545      97
 
PasI CC'CwG_GG

Cuts at:   1348    1348 
   Size:       5878
 
PciI A'CATG_T

Cuts at:   4777    4777 
   Size:       5878
 
PflMI CCAn_nnn'nTGG

Cuts at:   1108    2474    2915    3125    1108 
   Size:       1366     441     210    3861

  Fragments arranged by size: 

                3861    1366     441     210
 
PmeI GTTT'AAAC

Cuts at:   3182    3182 
   Size:       5878
 
PmlI CAC'GTG

Cuts at:   2558    2759    2954    3086    2558 
   Size:        201     195     132    5350

  Fragments arranged by size: 

                5350     201     195     132
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3947    3979    5460    5492    3947 
   Size:         32    1481      32    4333

  Fragments arranged by size: 

                4333    1481      32      32
 
PpuMI rG'GwC_Cy

Cuts at:   2366    2480    2366 
   Size:        114    5764
 
PshAI GACnn'nnGTC

Cuts at:   1613    1613 
   Size:       5878
 
PsiI TTA'TAA

Cuts at:   3448    3448 
   Size:       5878
 
PspOMI G'GGCC_C

Cuts at:   2900    2900 
   Size:       5878
 
PstI C_TGCA'G

Cuts at:    830    3230     830 
   Size:       2400    3478
 
PvuI CG_AT'CG

Cuts at:   2001    2001 
   Size:       5878
 
SacI G_AGCT'C

Cuts at:   2548    2548 
   Size:       5878
 
SacII CC_GC'GG

Cuts at:   3112    3112 
   Size:       5878
 
SalI G'TCGA_C

Cuts at:   1936    3220    1936 
   Size:       1284    4594
 
SapI GCTCTTCn'nnn_

Cuts at:   2039    2536    2039 
   Size:        497    5381
 
SbfI CC_TGCA'GG

Cuts at:   3230    3230 
   Size:       5878
 
ScaI AGT'ACT

Cuts at:   2115    4126    5838    2115 
   Size:       2011    1712    2155

  Fragments arranged by size: 

                2155    2011    1712
 
SfcI C'TryA_G

Cuts at:    826    1045    2845    3094    3226    4361    5042    5233     826 
   Size:        219    1800     249     132    1135     681     191    1471

  Fragments arranged by size: 

                1800    1471    1135     681     249     219     191     132
            
 
SgfI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5878
 
SgrAI Cr'CCGG_yG

Cuts at:   1700    1700 
   Size:       5878
 
SmaI CCC'GGG

Cuts at:   1872    3205    1872 
   Size:       1333    4545
 
SmlI C'TyrA_G

Cuts at:    820    1017    1943    2986    3010    3982    4883    5145     820 
   Size:        197     926    1043      24     972     901     262    1553

  Fragments arranged by size: 

                1553    1043     972     926     901     262     197      24
            
 
SnaBI TAC'GTA

Cuts at:    493    3669     493 
   Size:       3176    2702
 
SpeI A'CTAG_T

Cuts at:    152     152 
   Size:       5878
 
SphI G_CATG'C

Cuts at:   1132    3236    1132 
   Size:       2104    3774
 
SspI AAT'ATT

Cuts at:      5      52    3802       5 
   Size:         47    3750    2081

  Fragments arranged by size: 

                3750    2081      47
 
StuI AGG'CCT

Cuts at:   1517    1517 
   Size:       5878
 
StyI C'CwwG_G

Cuts at:    513    1065    2004    3317     513 
   Size:        552     939    1313    3074

  Fragments arranged by size: 

                3074    1313     939     552
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    325    1580    3905    3922    4075    4260    4705     325 
   Size:       1255    2325      17     153     185     445    1498

  Fragments arranged by size: 

                2325    1498    1255     445     185     153      17
 
TatI w'GTAC_w

Cuts at:     96     370     450     483     534    1977    2113    4124    5836 
   Size:        274      80      33      51    1443     136    2011    1712

Cuts at:   5836      96 
   Size:        138

  Fragments arranged by size: 

                2011    1712    1443     274     138     136      80      51
                  33
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   2091    2123    5838    5870    2091 
   Size:         32    3715      32    2099

  Fragments arranged by size: 

                3715    2099      32      32
 
Tth111I GACn'n_nGTC

Cuts at:   1316    1316 
   Size:       5878
 
XbaI T'CTAG_A

Cuts at:   3214    3214 
   Size:       5878
 
XhoI C'TCGA_G

Cuts at:   1943    1943 
   Size:       5878
 
XmaI C'CCGG_G

Cuts at:   1870    3203    1870 
   Size:       1333    4545
 
XmnI GAAnn'nnTTC

Cuts at:   4007    4007 
   Size:       5878
 
ZraI GAC'GTC

Cuts at:    276     329     412     598    1534    3686     276 
   Size:         53      83     186     936    2152    2468

  Fragments arranged by size: 

                2468    2152     936     186      83      53
 

 Enzymes that do cut:

    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI    AlwNI     ApaI    ApaLI     ApoI     AseI
    AsiSI     AvaI    BamHI     BanI    BanII     BbsI     BcgI    BciVI
     BclI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI
     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI
    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI Bsp1286I
    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII
    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI     BtgI    BtgZI
     BtsI     ClaI    CspCI     DraI   DraIII     DrdI     EaeI     EagI
     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI     FspI
    HaeII    Hin4I   HincII  HindIII     HpaI     KpnI     MfeI     MluI
     MmeI     MscI     MslI   MspA1I     NcoI     NdeI     NheI     NotI
     NspI     PasI     PciI    PflMI     PmeI     PmlI     PpiI    PpuMI
    PshAI     PsiI   PspOMI     PstI     PvuI     SacI    SacII     SalI
     SapI     SbfI     ScaI     SfcI     SgfI    SgrAI     SmaI     SmlI
    SnaBI     SpeI     SphI     SspI     StuI     StyI    TaqII     TatI
     TstI  Tth111I     XbaI     XhoI     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AarI     AfeI     AloI     AscI    AvrII     BaeI     BbeI    BbvCI
    BfrBI     BplI    BsiWI    BsmBI  BstZ17I   Bsu36I    EcoRV     FalI
     FseI    FspAI     KasI     NaeI     NarI   NgoMIV     NruI     NsiI
     PacI     PfoI    PspXI     PsrI    PvuII    RsrII    SanDI    SexAI
     SfiI     SfoI     SrfI     SwaI     XcmI
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