(Circular) (Six-base) MAPSORT of: pFN21AE4373.seq Check: 9538 from: 1 to: 5878 pFN21AE4373 5878 bp With 182 enzymes: SgfI * March 28, 2012 13:33 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3688 278 Size: 53 83 186 936 2152 2468 Fragments arranged by size: 2468 2152 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2281 3221 1937 Size: 344 940 4594 Fragments arranged by size: 4594 940 344 Acc65I G'GTAC_C Cuts at: 3072 3199 3072 Size: 127 5751 AclI AA'CG_TT Cuts at: 1563 4005 4378 1563 Size: 2442 373 3063 Fragments arranged by size: 3063 2442 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2490 3941 5325 5593 1632 Size: 858 1451 1384 268 1917 Fragments arranged by size: 1917 1451 1384 858 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5681 AflIII A'CryG_T Cuts at: 1203 4777 5754 1203 Size: 3574 977 1327 Fragments arranged by size: 3574 1327 977 AgeI A'CCGG_T Cuts at: 4709 4709 Size: 5878 AhdI GACnn_n'nnGTC Cuts at: 4607 4607 Size: 5878 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5878 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5878 AlwNI CAG_nnn'CTG Cuts at: 3621 5193 3621 Size: 1572 4306 ApaI G_GGCC'C Cuts at: 2904 2904 Size: 5878 ApaLI G'TGCA_C Cuts at: 3935 5091 3935 Size: 1156 4722 ApoI r'AATT_y Cuts at: 1493 2413 3187 3413 3565 5674 1493 Size: 920 774 226 152 2109 1697 Fragments arranged by size: 2109 1697 920 774 226 152 AseI AT'TA_AT Cuts at: 160 1032 4432 160 Size: 872 3400 1606 Fragments arranged by size: 3400 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5878 AvaI C'yCGr_G Cuts at: 1870 1943 3203 1870 Size: 73 1260 4545 Fragments arranged by size: 4545 1260 73 BamHI G'GATC_C Cuts at: 3208 3575 3208 Size: 367 5511 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3072 3199 4654 618 Size: 325 217 627 1285 127 1455 1842 Fragments arranged by size: 1842 1455 1285 627 325 217 127 BanII G_rGCy'C Cuts at: 1391 2548 2904 3197 1391 Size: 1157 356 293 4072 Fragments arranged by size: 4072 1157 356 293 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4907 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4069 4103 1903 Size: 34 2132 34 3678 Fragments arranged by size: 3678 2132 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3770 4980 3770 Size: 1210 4668 BclI T'GATC_A Cuts at: 1548 1548 Size: 5878 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4489 136 Size: 107 122 71 631 605 2817 1525 Fragments arranged by size: 2817 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2495 5873 2495 Size: 3378 2500 BlpI GC'TnA_GC Cuts at: 3295 3295 Size: 5878 Bme1580I G_kGCm'C Cuts at: 1790 2904 3939 5095 1790 Size: 1114 1035 1156 2573 Fragments arranged by size: 2573 1156 1114 1035 BmgBI CAC'GTC Cuts at: 1318 2285 1318 Size: 967 4911 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2441 3609 4567 Size: 416 53 83 399 24 1024 1168 958 Cuts at: 4567 442 Size: 1753 Fragments arranged by size: 1753 1168 1024 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5878 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4538 1239 Size: 3299 2579 Bpu10I CC'TnA_GC Cuts at: 2190 2190 Size: 5878 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2971 2995 4003 4868 5166 2971 Size: 24 1008 865 298 3683 Fragments arranged by size: 3683 1008 865 298 24 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4541 882 Size: 617 3042 2219 Fragments arranged by size: 3042 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2558 2759 2879 2954 3086 3669 493 Size: 711 1354 201 120 75 132 583 2702 Fragments arranged by size: 2702 1354 711 583 201 132 120 75 BsaBI GATnn'nnATC Cuts at: 2766 5872 2766 Size: 3106 2772 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3685 4067 4702 275 Size: 53 83 186 936 2152 382 635 1451 Fragments arranged by size: 2152 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4311 4709 4983 5130 5541 4311 Size: 398 274 147 411 4648 Fragments arranged by size: 4648 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5653 Fragments arranged by size: 5653 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2358 2475 3069 1186 Size: 1172 117 594 3995 Fragments arranged by size: 3995 1172 594 117 BseYI C'CCAG_C Cuts at: 2179 5081 5577 2179 Size: 2902 496 2480 Fragments arranged by size: 2902 2480 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2484 1163 Size: 573 748 4557 Fragments arranged by size: 4557 748 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3328 4089 5117 1540 Size: 275 186 1327 761 1028 2301 Fragments arranged by size: 2301 1327 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2548 2887 3118 3939 4024 5095 2548 Size: 339 231 821 85 1071 3331 Fragments arranged by size: 3331 1071 821 339 231 85 BsmI GAATG_Cn' Cuts at: 3389 3482 3389 Size: 93 5785 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2548 2887 2904 3118 3197 3939 4024 Size: 399 758 339 17 214 79 742 85 Cuts at: 4024 5095 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 758 742 399 339 214 85 79 17 BspEI T'CCGG_A Cuts at: 5541 5541 Size: 5878 BspHI T'CATG_A Cuts at: 3767 5497 3767 Size: 1730 4148 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3233 844 Size: 2389 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3765 1394 Size: 429 1942 3507 Fragments arranged by size: 3507 1942 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4373 4547 65 Size: 1183 3125 174 1396 Fragments arranged by size: 3125 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4522 4709 1700 Size: 110 2712 187 2869 Fragments arranged by size: 2869 2712 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5878 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5878 BssSI C'ACGA_G Cuts at: 2688 2718 2895 3938 4950 2688 Size: 30 177 1043 1012 3616 Fragments arranged by size: 3616 1043 1012 177 30 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5878 BstBI TT'CG_AA Cuts at: 4682 4682 Size: 5878 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5878 BstXI CCAn_nnnn'nTGG Cuts at: 2245 2245 Size: 5878 BstYI r'GATC_y Cuts at: 1972 2495 3208 3575 3960 3977 5429 5515 5527 Size: 523 713 367 385 17 1452 86 12 Cuts at: 5527 5873 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 713 523 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 3109 3133 513 Size: 552 939 1105 24 3258 Fragments arranged by size: 3258 1105 939 552 24 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5139 Fragments arranged by size: 5139 683 56 BtsI GCAGTG_nn' Cuts at: 2533 3394 4188 4208 2533 Size: 861 794 20 4203 Fragments arranged by size: 4203 861 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5878 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5843 DraI TTT'AAA Cuts at: 3182 3182 Size: 5878 DraIII CAC_nnn'GTG Cuts at: 2882 2882 Size: 5878 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4885 809 Size: 507 3569 1802 Fragments arranged by size: 3569 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3130 3325 4214 Size: 54 1425 50 275 18 1300 195 889 Cuts at: 4214 5843 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1300 889 275 195 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3325 1537 Size: 275 1513 4090 Fragments arranged by size: 4090 1513 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2039 2335 2536 2557 3808 1353 Size: 686 296 201 21 1251 3423 Fragments arranged by size: 3423 1251 686 296 201 21 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4465 4839 4985 387 Size: 1064 138 2876 374 146 1280 Fragments arranged by size: 2876 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2546 2546 Size: 5878 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2490 3941 4538 5325 5593 1239 Size: 393 858 1451 597 787 268 1524 Fragments arranged by size: 1524 1451 858 787 597 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2322 2895 2322 Size: 573 5305 EcoO109I rG'GnC_Cy Cuts at: 2315 2366 2480 2900 2901 2315 Size: 51 114 420 1 5292 Fragments arranged by size: 5292 420 114 51 1 EcoRI G'AATT_C Cuts at: 3187 3565 3187 Size: 378 5500 FspI TGC'GCA Cuts at: 4384 4384 Size: 5878 HaeII r_GCGC'y Cuts at: 1678 5025 1678 Size: 3347 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2756 2788 2969 2993 3001 Size: 32 135 32 1184 32 181 24 8 Cuts at: 3001 3025 4519 4551 4593 4625 1373 Size: 24 1494 32 42 32 2626 Fragments arranged by size: 2626 1494 1184 181 135 42 32 32 32 32 32 24 24 8 HincII GTy'rAC Cuts at: 669 1657 1938 3222 3468 669 Size: 988 281 1284 246 3079 Fragments arranged by size: 3079 1284 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5878 HpaI GTT'AAC Cuts at: 3468 3468 Size: 5878 KpnI G_GTAC'C Cuts at: 3076 3203 3076 Size: 127 5751 MfeI C'AATT_G Cuts at: 3477 3477 Size: 5878 MluI A'CGCG_T Cuts at: 5754 5754 Size: 5878 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4992 5176 1305 Size: 3687 184 2007 Fragments arranged by size: 3687 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4056 Fragments arranged by size: 4056 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2243 2391 2832 3836 4195 4354 518 Size: 648 1077 148 441 1004 359 159 2042 Fragments arranged by size: 2042 1077 1004 648 441 359 159 148 MspA1I CmG'CkG Cuts at: 1606 2599 3111 3294 3971 5119 5364 1606 Size: 993 512 183 677 1148 245 2120 Fragments arranged by size: 2120 1148 993 677 512 245 183 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4387 Fragments arranged by size: 4387 939 552 NdeI CA'TA_TG Cuts at: 387 2857 387 Size: 2470 3408 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5878 NotI GC'GGCC_GC Cuts at: 3325 3325 Size: 5878 NspI r_CATG'y Cuts at: 1132 1229 3236 4781 1132 Size: 97 2007 1545 2229 Fragments arranged by size: 2229 2007 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5878 PciI A'CATG_T Cuts at: 4777 4777 Size: 5878 PflMI CCAn_nnn'nTGG Cuts at: 1108 2474 2915 3125 1108 Size: 1366 441 210 3861 Fragments arranged by size: 3861 1366 441 210 PmeI GTTT'AAAC Cuts at: 3182 3182 Size: 5878 PmlI CAC'GTG Cuts at: 2558 2759 2954 3086 2558 Size: 201 195 132 5350 Fragments arranged by size: 5350 201 195 132 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3947 3979 5460 5492 3947 Size: 32 1481 32 4333 Fragments arranged by size: 4333 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2366 2480 2366 Size: 114 5764 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5878 PsiI TTA'TAA Cuts at: 3448 3448 Size: 5878 PspOMI G'GGCC_C Cuts at: 2900 2900 Size: 5878 PstI C_TGCA'G Cuts at: 830 3230 830 Size: 2400 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5878 SacI G_AGCT'C Cuts at: 2548 2548 Size: 5878 SacII CC_GC'GG Cuts at: 3112 3112 Size: 5878 SalI G'TCGA_C Cuts at: 1936 3220 1936 Size: 1284 4594 SapI GCTCTTCn'nnn_ Cuts at: 2039 2536 2039 Size: 497 5381 SbfI CC_TGCA'GG Cuts at: 3230 3230 Size: 5878 ScaI AGT'ACT Cuts at: 2115 4126 5838 2115 Size: 2011 1712 2155 Fragments arranged by size: 2155 2011 1712 SfcI C'TryA_G Cuts at: 826 1045 2845 3094 3226 4361 5042 5233 826 Size: 219 1800 249 132 1135 681 191 1471 Fragments arranged by size: 1800 1471 1135 681 249 219 191 132 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5878 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5878 SmaI CCC'GGG Cuts at: 1872 3205 1872 Size: 1333 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2986 3010 3982 4883 5145 820 Size: 197 926 1043 24 972 901 262 1553 Fragments arranged by size: 1553 1043 972 926 901 262 197 24 SnaBI TAC'GTA Cuts at: 493 3669 493 Size: 3176 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5878 SphI G_CATG'C Cuts at: 1132 3236 1132 Size: 2104 3774 SspI AAT'ATT Cuts at: 5 52 3802 5 Size: 47 3750 2081 Fragments arranged by size: 3750 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5878 StyI C'CwwG_G Cuts at: 513 1065 2004 3317 513 Size: 552 939 1313 3074 Fragments arranged by size: 3074 1313 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3905 3922 4075 4260 4705 325 Size: 1255 2325 17 153 185 445 1498 Fragments arranged by size: 2325 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2113 4124 5836 Size: 274 80 33 51 1443 136 2011 1712 Cuts at: 5836 96 Size: 138 Fragments arranged by size: 2011 1712 1443 274 138 136 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2091 2123 5838 5870 2091 Size: 32 3715 32 2099 Fragments arranged by size: 3715 2099 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5878 XbaI T'CTAG_A Cuts at: 3214 3214 Size: 5878 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5878 XmaI C'CCGG_G Cuts at: 1870 3203 1870 Size: 1333 4545 XmnI GAAnn'nnTTC Cuts at: 4007 4007 Size: 5878 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3686 276 Size: 53 83 186 936 2152 2468 Fragments arranged by size: 2468 2152 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SacI SacII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PspXI PsrI PvuII RsrII SanDI SexAI SfiI SfoI SrfI SwaI XcmI