Restriction map for
pFN21AE5122
(Circular) (Six-base) MAPSORT of: pFN21AE5122.seq Check: 3197 from: 1 to: 5044
pFN21AE5122 5044 bp
With 182 enzymes: SgfI *
December 8, 2014 13:45 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2854 278
Size: 53 83 186 936 1318 2468
Fragments arranged by size:
2468 1318 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2387 1937
Size: 450 4594
Acc65I G'GTAC_C
Cuts at: 2365 2365
Size: 5044
AclI AA'CG_TT
Cuts at: 1563 3171 3544 1563
Size: 1608 373 3063
Fragments arranged by size:
3063 1608 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2096 3107 4491 4759 1632
Size: 464 1011 1384 268 1917
Fragments arranged by size:
1917 1384 1011 464 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 4847
AflIII A'CryG_T
Cuts at: 1203 2088 3943 4920 1203
Size: 885 1855 977 1327
Fragments arranged by size:
1855 1327 977 885
AgeI A'CCGG_T
Cuts at: 3875 3875
Size: 5044
AhdI GACnn_n'nnGTC
Cuts at: 3773 3773
Size: 5044
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5044
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2330 1184
Size: 1146 3898
AlwNI CAG_nnn'CTG
Cuts at: 2074 2787 4359 2074
Size: 713 1572 2759
Fragments arranged by size:
2759 1572 713
ApaLI G'TGCA_C
Cuts at: 3101 4257 3101
Size: 1156 3888
ApoI r'AATT_y
Cuts at: 1493 2353 2579 2731 4840 1493
Size: 860 226 152 2109 1697
Fragments arranged by size:
2109 1697 860 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3598 160
Size: 872 2566 1606
Fragments arranged by size:
2566 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5044
AvaI C'yCGr_G
Cuts at: 1870 1943 2176 2369 1870
Size: 73 233 193 4545
Fragments arranged by size:
4545 233 193 73
BamHI G'GATC_C
Cuts at: 2374 2741 2374
Size: 367 4677
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2365 3820 618
Size: 325 217 627 578 1455 1842
Fragments arranged by size:
1842 1455 627 578 325 217
BanII G_rGCy'C
Cuts at: 1391 2060 2363 1391
Size: 669 303 4072
Fragments arranged by size:
4072 669 303
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4073
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3235 3269 1903
Size: 34 1298 34 3678
Fragments arranged by size:
3678 1298 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2936 4146 2936
Size: 1210 3834
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5044
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 3655 136
Size: 107 122 71 631 605 337 1646 1525
Fragments arranged by size:
1646 1525 631 605 337 122 107 71
BglII A'GATC_T
Cuts at: 5039 5039
Size: 5044
BlpI GC'TnA_GC
Cuts at: 2461 2461
Size: 5044
Bme1580I G_kGCm'C
Cuts at: 1790 3105 4261 1790
Size: 1315 1156 2573
Fragments arranged by size:
2573 1315 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5044
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2775 3733 442
Size: 416 53 83 399 24 1358 958 1753
Fragments arranged by size:
1753 1358 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5044
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2079 2079
Size: 5044
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3704 1239
Size: 2465 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2074 3169 4034 4332 2074
Size: 1095 865 298 2786
Fragments arranged by size:
2786 1095 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3707 882
Size: 617 2208 2219
Fragments arranged by size:
2219 2208 617
BsaAI yAC'GTr
Cuts at: 493 1204 2835 493
Size: 711 1631 2702
Fragments arranged by size:
2702 1631 711
BsaBI GATnn'nnATC
Cuts at: 5038 5038
Size: 5044
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2012 2851 3233 3868
Size: 53 83 186 936 479 839 382 635
Cuts at: 3868 275
Size: 1451
Fragments arranged by size:
1451 936 839 635 479 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3477 3875 4149 4296 4707 3477
Size: 398 274 147 411 3814
Fragments arranged by size:
3814 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2042 2072 1178
Size: 30 165 30 639 30 4150
Fragments arranged by size:
4150 639 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2132 1186
Size: 946 4098
BseYI C'CCAG_C
Cuts at: 4247 4743 4247
Size: 496 4548
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2300 1163
Size: 573 564 3907
Fragments arranged by size:
3907 573 564
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2494 3255 4283 1540
Size: 275 186 493 761 1028 2301
Fragments arranged by size:
2301 1028 761 493 275 186
BsiHKAI G_wGCw'C
Cuts at: 2060 3105 3190 4261 2060
Size: 1045 85 1071 2843
Fragments arranged by size:
2843 1071 1045 85
BsmI GAATG_Cn'
Cuts at: 2555 2648 2555
Size: 93 4951
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2060 2363 3105 3190 4261 1391
Size: 399 270 303 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 303 270 85
BspEI T'CCGG_A
Cuts at: 4707 4707
Size: 5044
BspHI T'CATG_A
Cuts at: 2328 2933 4663 2328
Size: 605 1730 2709
Fragments arranged by size:
2709 1730 605
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2103 2399 844
Size: 1259 296 3489
Fragments arranged by size:
3489 1259 296
BsrBI CCG'CTC
Cuts at: 1394 1823 2931 1394
Size: 429 1108 3507
Fragments arranged by size:
3507 1108 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3539 3713 65
Size: 1183 2291 174 1396
Fragments arranged by size:
2291 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3688 3875 1700
Size: 110 1878 187 2869
Fragments arranged by size:
2869 1878 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5044
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5044
BssSI C'ACGA_G
Cuts at: 2144 3104 4116 2144
Size: 960 1012 3072
Fragments arranged by size:
3072 1012 960
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5044
BstBI TT'CG_AA
Cuts at: 3848 3848
Size: 5044
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5044
BstYI r'GATC_y
Cuts at: 1972 2374 2741 3126 3143 4595 4681 4693 5039
Size: 402 367 385 17 1452 86 12 346
Cuts at: 5039 1972
Size: 1977
Fragments arranged by size:
1977 1452 402 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 4492
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4305
Fragments arranged by size:
4305 683 56
BtsI GCAGTG_nn'
Cuts at: 2560 3354 3374 2560
Size: 794 20 4230
Fragments arranged by size:
4230 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5044
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5009
DraI TTT'AAA
Cuts at: 2348 2348
Size: 5044
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4051 809
Size: 507 2735 1802
Fragments arranged by size:
2735 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2491 3380 5009
Size: 54 1425 50 275 18 661 889 1629
Cuts at: 5009 8
Size: 43
Fragments arranged by size:
1629 1425 889 661 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2491 1537
Size: 275 679 4090
Fragments arranged by size:
4090 679 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2040 2974 1353
Size: 687 934 3423
Fragments arranged by size:
3423 934 687
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3631 4005 4151 387
Size: 1064 138 2042 374 146 1280
Fragments arranged by size:
2042 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2058 2058
Size: 5044
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2096 3107 3704 4491 4759 1239
Size: 393 464 1011 597 787 268 1524
Fragments arranged by size:
1524 1011 787 597 464 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2151 2151
Size: 5044
EcoRI G'AATT_C
Cuts at: 2353 2731 2353
Size: 378 4666
FspI TGC'GCA
Cuts at: 3550 3550
Size: 5044
HaeII r_GCGC'y
Cuts at: 1678 2123 4191 1678
Size: 445 2068 2531
Fragments arranged by size:
2531 2068 445
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3685 3717 3759 3791 1373
Size: 32 135 32 2113 32 42 32 2626
Fragments arranged by size:
2626 2113 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2388 2634 669
Size: 988 281 450 246 3079
Fragments arranged by size:
3079 988 450 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5044
HpaI GTT'AAC
Cuts at: 2634 2634
Size: 5044
KpnI G_GTAC'C
Cuts at: 2369 2369
Size: 5044
MfeI C'AATT_G
Cuts at: 2643 2643
Size: 5044
MluI A'CGCG_T
Cuts at: 2088 4920 2088
Size: 2832 2212
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4158 4342 1305
Size: 2853 184 2007
Fragments arranged by size:
2853 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3222
Fragments arranged by size:
3222 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3002 3361 3520 518
Size: 648 1836 359 159 2042
Fragments arranged by size:
2042 1836 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2460 3137 4285 4530 1606
Size: 854 677 1148 245 2120
Fragments arranged by size:
2120 1148 854 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4492
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5044
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5044
NotI GC'GGCC_GC
Cuts at: 2491 2491
Size: 5044
NspI r_CATG'y
Cuts at: 1132 1229 2402 3947 1132
Size: 97 1173 1545 2229
Fragments arranged by size:
2229 1545 1173 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5044
PciI A'CATG_T
Cuts at: 3943 3943
Size: 5044
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5044
PmeI GTTT'AAAC
Cuts at: 2348 2348
Size: 5044
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3113 3145 4626 4658 3113
Size: 32 1481 32 3499
Fragments arranged by size:
3499 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2151 2151
Size: 5044
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5044
PsiI TTA'TAA
Cuts at: 2614 2614
Size: 5044
PstI C_TGCA'G
Cuts at: 830 2127 2396 830
Size: 1297 269 3478
Fragments arranged by size:
3478 1297 269
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5044
SacI G_AGCT'C
Cuts at: 2060 2060
Size: 5044
SalI G'TCGA_C
Cuts at: 1936 2386 1936
Size: 450 4594
SbfI CC_TGCA'GG
Cuts at: 2396 2396
Size: 5044
ScaI AGT'ACT
Cuts at: 2051 3292 5004 2051
Size: 1241 1712 2091
Fragments arranged by size:
2091 1712 1241
SfcI C'TryA_G
Cuts at: 826 1045 2123 2392 3527 4208 4399 826
Size: 219 1078 269 1135 681 191 1471
Fragments arranged by size:
1471 1135 1078 681 269 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5044
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5044
SmaI CCC'GGG
Cuts at: 1872 2371 1872
Size: 499 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2053 3148 4049 4311 820
Size: 197 926 110 1095 901 262 1553
Fragments arranged by size:
1553 1095 926 901 262 197 110
SnaBI TAC'GTA
Cuts at: 493 2835 493
Size: 2342 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5044
SphI G_CATG'C
Cuts at: 1132 2402 1132
Size: 1270 3774
SspI AAT'ATT
Cuts at: 5 52 2968 5
Size: 47 2916 2081
Fragments arranged by size:
2916 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5044
StyI C'CwwG_G
Cuts at: 513 1065 2260 2483 513
Size: 552 1195 223 3074
Fragments arranged by size:
3074 1195 552 223
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3071 3088 3241 3426 3871 325
Size: 1255 1491 17 153 185 445 1498
Fragments arranged by size:
1498 1491 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2049 3290 5002
Size: 274 80 33 51 1443 72 1241 1712
Cuts at: 5002 96
Size: 138
Fragments arranged by size:
1712 1443 1241 274 138 80 72 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5004 5036 5004
Size: 32 5012
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5044
XbaI T'CTAG_A
Cuts at: 2380 2380
Size: 5044
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5044
XmaI C'CCGG_G
Cuts at: 1870 2369 1870
Size: 499 4545
XmnI GAAnn'nnTTC
Cuts at: 3173 3173
Size: 5044
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2852 276
Size: 53 83 186 936 1318 2468
Fragments arranged by size:
2468 1318 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PmeI PpiI PpuMI PshAI PsiI PstI PvuI SacI
SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I
DraIII EcoNI EcoRV FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI
PspXI PsrI PvuII RsrII SacII SanDI SapI SexAI
SfiI SfoI SrfI SwaI XcmI