################################## # NO CUT ENZYMES ( 138 enzymes) # ################################## AatII Acc65I AccIII AclI AcyI AflII AflIII AgeI AhdI Alw44I AlwNI Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AspHI AvaII AviII AvrII BanI BanII BbeI BbrPI BbvCI BcgI BciVI BclI BcnI BfrI BglI BlnI BlpI Bpu1102I BsaAI BsaBI BsaHI BsaI BsaWI BseAI BseRI BsgI BsiEI BsiHKAI BsiWI BsmBI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrGI BssHII BssSI BstBI BstEII BstPI BstXI CelII CfoI Cfr13I ClaI CpoI DraII DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoO109I EcoO65I EcoRV FauI FbaI FseI FspI HaeII HgaI HhaI Hin1I HinP1I HpaI HphI KasI KpnI KspI MamI MluI MroI NaeI NarI NciI NgoMIV NheI NotI NruI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI RcaI RsrII SacI SacII Sau96I SbfI ScaI SfiI SfoI SfuI SgrAI SmaI SnaBI Sse8387I SspBI StuI Tsp45I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 49 enzymes) # ################################### AccII 63 CGCG CG^CG AocI 633 CCTGAGG CC^T[ACGT]AGG Asp700I 1015 GAAGCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspI 325 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 338 CCCGAG C^[CT]CG[GA]G BamHI 448 GGATCC G^GATCC BbsI 58 GTCTTC GAAGAC(2/6) BglII 297 AGATCT A^GATCT BmyI 1167 GGGCAC G[AGT]GC[ACT]^C BpmI 1097 CTGGAG CTGGAG(16/14) BpuAI 58 GTCTTC GAAGAC(2/6) BsaJI 1068 CCATGG C^C[ACGT][ACGT]GG BsmFI 1106 GGGAC GGGAC(10/14) BsmI 927 GCATTC GAATGC(1/-1) BsoBI 338 CCCGAG C^[CT]CG[GA]G Bsp1286I 1167 GGGCAC G[AGT]GC[ACT]^C BsrBI 258 GAGCGG CCGCTC(-3/-3) BsrDI 745 CATTGC GCAATG(2/0) Bst1107I 1056 GTATAC GTA^TAC BstAPI 1018 GCAGTTCGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstUI 63 CGCG CG^CG BstZ17I 1056 GTATAC GTA^TAC Bsu36I 633 CCTGAGG CC^T[ACGT]AGG DsaI 1068 CCATGG C^C[GA][CT]GG Eco81I 633 CCTGAGG CC^T[ACGT]AGG EcoRI 263 GAATTC G^AATTC EcoT14I 1068 CCATGG C^C[AT][AT]GG HincII 229 GTCGAC GT[CT]^[GA]AC HindII 229 GTCGAC GT[CT]^[GA]AC MaeII 290 ACGT A^CGT MfeI 1148 CAATTG C^AATTG MunI 1148 CAATTG C^AATTG MvnI 63 CGCG CG^CG NcoI 1068 CCATGG C^CATGG NlaIV 448 GGATCC GG[ACGT]^[ACGT]CC PflFI 325 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC PleI 850 GAGTC GAGTC(4/5) PvuII 173 CAGCTG CAG^CTG SalI 229 GTCGAC G^TCGAC SapI 91 GCTCTTC GCTCTTC(1/4) SexAI 1051 ACCAGGT A^CC[AT]GGT SmlI 885 CTTGAG C^T[CT][GA]AG SpeI 1039 ACTAGT A^CTAGT SphI 923 GCATGC GCATG^C SspI 1136 AATATT AAT^ATT StyI 1068 CCATGG C^C[AT][AT]GG SwaI 248 ATTTAAAT ATTT^AAAT Tth111I 325 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC XmnI 1015 GAAGCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 28 enzymes) # ################################### AccI 229 GTCGAC GT^[AC][GT]AC 1056 GTATAC AcsI 263 GAATTC [GA]^AATT[CT] 400 GAATTT ApoI 263 GAATTC [GA]^AATT[CT] 400 GAATTT BalI 75 TGGCCA TGG^CCA 1065 TGGCCA BsmAI 999 GTCTC GTCTC(1/5) 1234 GTCTC BsrFI 165 GCCGGT [GA]^CCGG[CT] 660 GCCGGT BssKI 1052 CCAGG ^CC[ACGT]GG 1096 CCTGG BstNI 1052 CCAGG CC^[AT]GG 1096 CCTGG Cac8I 282 GCTGGC GC[ACGT]^[ACGT]GC 923 GCATGC Cfr10I 165 GCCGGT [GA]^CCGG[CT] 660 GCCGGT DraI 249 TTTAAA TTT^AAA 480 TTTAAA EaeI 75 TGGCCA [CT]^GGCC[GA] 1065 TGGCCA EarI 40 CTCTTC CTCTTC(1/4) 92 CTCTTC EcoRII 1052 CCAGG ^CC[AT]GG 1096 CCTGG EcoT22I 218 ATGCAT ATGCA^T 925 ATGCAT HindIII 4 AAGCTT A^AGCTT 1220 AAGCTT Ksp632I 40 CTCTTC CTCTTC(1/4) 92 CTCTTC MaeIII 169 GTAAC ^GT[ACGT]AC 1196 GTTAC MluNI 75 TGGCCA TGG^CCA 1065 TGGCCA MscI 75 TGGCCA TGG^CCA 1065 TGGCCA MspA1I 173 CAGCTG C[AC]G^C[GT]G 1229 CCGCTG MvaI 1052 CCAGG CC^[AT]GG 1096 CCTGG NdeI 143 CATATG CA^TATG 519 CATATG NsiI 218 ATGCAT ATGCA^T 925 ATGCAT PflMI 545 CCAGAGAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 782 CCAAACACTGG ScrFI 1052 CCAGG CC^[ACGT]GG 1096 CCTGG SfcI 124 CTACAG C^T[GA][CT]AG 1212 CTGTAG Van91I 545 CCAGAGAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 782 CCAAACACTGG ######################################### # 3 OR MORE CUT ENZYMES ( 43 enzymes) # ######################################### AciI AfaI AluI AlwI BbvI BfaI BsiYI BslI BsrI BstF5I BstYI DdeI DpnII Fnu4HI FokI HaeIII HapII HinfI HpaII ItaI MaeI MboI MboII MflI MnlI MseI MslI MspI MwoI NdeII NlaIII NspI RsaI Sau3AI SfaNI TaqI TfiI Tru9I TseI Tsp509I TspRI TthHB8I XhoII