################################## # NO CUT ENZYMES ( 165 enzymes) # ################################## Acc65I AccI AccII AccIII AclI AcsI AfaI AflII AflIII AgeI AhdI AluI Alw44I AlwNI AocI Aor51HI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AvaI AviII AvrII BamHI BbrPI BbsI BbvCI BcgI BciVI BclI BfrI BglI BglII BlnI BlpI BpmI Bpu1102I BpuAI BsaAI BsaWI BseAI BsgI BsiEI BsiWI BsmBI BsoBI Bsp1407I BspDI BspEI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BssHII Bst1107I BstAPI BstBI BstEII BstPI BstUI BstYI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DdeI DraI DsaI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT14I EcoT22I FauI FbaI FseI FspI HgaI HindIII HpaI HphI KpnI KspI MfeI MflI MluI MroI MseI MspA1I MunI MvnI NaeI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RsaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StyI SwaI TaqI Tru9I Tsp509I TthHB8I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 49 enzymes) # ################################### AatII 123 GACGTC GACGT^C AlwI 135 GATCC GGATC(4/5) AspHI 76 GAGCAC G[AT]GC[AT]^C AspI 166 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaII 179 GGTCC G^G[AT]CC BalI 42 TGGCCA TGG^CCA BanI 99 GGCGCC G^G[CT][GA]CC BbeI 99 GGCGCC GGCGC^C BcnI 281 CCGGG CC^[GC]GG BfaI 251 CTAG C^TAG BsaBI 135 GATCCACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaI 151 GAGACC GGTCTC(1/5) BseRI 73 GAGGAG GAGGAG(10/8) BsiHKAI 76 GAGCAC G[AT]GC[AT]^C BsiYI 330 CCTGATTCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 330 CCTGATTCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BsmAI 151 GAGAC GTCTC(1/5) BsmI 229 GCATTC GAATGC(1/-1) BsrI 373 CCAGT ACTGG(1/-1) BssSI 79 CACGAG CACGAG(-5/-1) BstXI 387 CCAGGCCTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Cac8I 44 GCCAGC GC[ACGT]^[ACGT]GC CfoI 100 GCGC GCG^C DraIII 79 CACGAGGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 166 GACCATGTCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EaeI 42 TGGCCA [CT]^GGCC[GA] EarI 196 CTCTTC CTCTTC(1/4) HaeII 99 GGCGCC [GA]GCGC^[CT] HapII 281 CCGG C^CGG HhaI 100 GCGC GCG^C HinP1I 100 GCGC G^CGC HincII 34 GTCAAC GT[CT]^[GA]AC HindII 34 GTCAAC GT[CT]^[GA]AC HpaII 281 CCGG C^CGG KasI 99 GGCGCC G^GCGCC Ksp632I 196 CTCTTC CTCTTC(1/4) MaeI 251 CTAG C^TAG MaeIII 277 GTGAC ^GT[ACGT]AC MamI 135 GATCCACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MluNI 42 TGGCCA TGG^CCA MscI 42 TGGCCA TGG^CCA MspI 281 CCGG C^CGG NarI 99 GGCGCC GG^CGCC NciI 281 CCGGG CC^[GC]GG PflFI 166 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC SfoI 99 GGCGCC GGC^GCC SmlI 67 CTCAAG C^T[CT][GA]AG Tsp45I 277 GTGAC ^GT[GC]AC Tth111I 166 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### AciI 144 CCGC CCGC(-3/-1) 305 CCGC AcyI 99 GGCGCC G[GA]^CG[CT]C 123 GACGTC ApaI 284 GGGCCC GGGCC^C 326 GGGCCC BanII 284 GGGCCC G[GA]GC[CT]^C 326 GGGCCC BbvI 146 GCAGC GCAGC(8/12) 212 GCTGC BsaHI 99 GGCGCC G[GA]^CG[CT]C 123 GACGTC BsmFI 254 GGGAC GGGAC(10/14) 180 GTCCC BspHI 200 TCATGA T^CATGA 347 TCATGA DpnII 135 GATC ^GATC 381 GATC DraII 283 GGGGCCC [GA]G^G[ACGT]CC[CT] 326 GGGCCCT EcoO109I 283 GGGGCCC [GA]G^G[ACGT]CC[CT] 326 GGGCCCT Fnu4HI 146 GCAGC GC^[ACGT]GC 212 GCTGC Hin1I 99 GGCGCC G[GA]^CG[CT]C 123 GACGTC HinfI 3 GAATC G^A[ACGT]TC 333 GATTC ItaI 146 GCAGC GC^[ACGT]GC 212 GCTGC MaeII 124 ACGT A^CGT 275 ACGT MboI 135 GATC ^GATC 381 GATC MslI 342 CATCCTCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 369 CATCCCAGTG NdeII 135 GATC ^GATC 381 GATC RcaI 200 TCATGA T^CATGA 347 TCATGA Sau3AI 135 GATC ^GATC 381 GATC SfaNI 63 GATGC GCATC(5/9) 341 GCATC StuI 290 AGGCCT AGG^CCT 389 AGGCCT TfiI 3 GAATC G^A[AT]TC 333 GATTC TseI 146 GCAGC G^C[AT]GC 212 GCTGC ######################################### # 3 OR MORE CUT ENZYMES ( 19 enzymes) # ######################################### BmyI BsaJI Bsp1286I BssKI BstF5I BstNI Cfr13I EcoRII FokI HaeIII MboII MnlI MvaI MwoI NlaIII NlaIV Sau96I ScrFI TspRI