(Circular) (Six-base) MAPSORT of: pFN21AE1483.seq Check: 2916 from: 1 to: 6058 pFN21AE1483 6058 bp With 182 enzymes: SgfI * August 16, 2010 14:52 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3868 278 Size: 53 83 186 936 2332 2468 Fragments arranged by size: 2468 2332 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2208 2616 3401 1937 Size: 271 408 785 4594 Fragments arranged by size: 4594 785 408 271 Acc65I G'GTAC_C Cuts at: 3379 3379 Size: 6058 AclI AA'CG_TT Cuts at: 1563 4185 4558 1563 Size: 2622 373 3063 Fragments arranged by size: 3063 2622 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2146 4121 5505 5773 1632 Size: 514 1975 1384 268 1917 Fragments arranged by size: 1975 1917 1384 514 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5861 AflIII A'CryG_T Cuts at: 1203 4957 5934 1203 Size: 3754 977 1327 Fragments arranged by size: 3754 1327 977 AgeI A'CCGG_T Cuts at: 4889 4889 Size: 6058 AhdI GACnn_n'nnGTC Cuts at: 4787 4787 Size: 6058 AleI CACnn'nnGTG Cuts at: 1166 3089 1166 Size: 1923 4135 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2204 1184 Size: 1020 5038 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2076 2108 2076 Size: 32 6026 AlwNI CAG_nnn'CTG Cuts at: 3801 5373 3801 Size: 1572 4486 ApaLI G'TGCA_C Cuts at: 2389 4115 5271 2389 Size: 1726 1156 3176 Fragments arranged by size: 3176 1726 1156 ApoI r'AATT_y Cuts at: 1493 2367 2765 3367 3593 3745 5854 1493 Size: 874 398 602 226 152 2109 1697 Fragments arranged by size: 2109 1697 874 602 398 226 152 AseI AT'TA_AT Cuts at: 160 1032 4612 160 Size: 872 3580 1606 Fragments arranged by size: 3580 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6058 AvaI C'yCGr_G Cuts at: 1870 1943 2193 2316 3383 1870 Size: 73 250 123 1067 4545 Fragments arranged by size: 4545 1067 250 123 73 BamHI G'GATC_C Cuts at: 3388 3755 3388 Size: 367 5691 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2246 2524 2782 3042 3091 Size: 325 217 627 459 278 258 260 49 Cuts at: 3091 3379 4834 618 Size: 288 1455 1842 Fragments arranged by size: 1842 1455 627 459 325 288 278 260 258 217 49 BanII G_rGCy'C Cuts at: 1391 2073 2556 2746 3172 3377 1391 Size: 682 483 190 426 205 4072 Fragments arranged by size: 4072 682 483 426 205 190 BbeI G_GCGC'C Cuts at: 2528 2528 Size: 6058 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5087 BbvCI CC'TCA_GC Cuts at: 3159 3159 Size: 6058 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4249 4283 1903 Size: 34 50 34 2228 34 3678 Fragments arranged by size: 3678 2228 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3950 5160 3950 Size: 1210 4848 BclI T'GATC_A Cuts at: 1548 1548 Size: 6058 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3051 4669 136 Size: 107 122 71 631 605 1379 1618 1525 Fragments arranged by size: 1618 1525 1379 631 605 122 107 71 BglII A'GATC_T Cuts at: 6053 6053 Size: 6058 BlpI GC'TnA_GC Cuts at: 3137 3475 3137 Size: 338 5720 Bme1580I G_kGCm'C Cuts at: 1790 2193 2325 2393 2787 3047 4119 5275 1790 Size: 403 132 68 394 260 1072 1156 2573 Fragments arranged by size: 2573 1156 1072 403 394 260 132 68 BmgBI CAC'GTC Cuts at: 1318 2971 1318 Size: 1653 4405 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3789 4747 442 Size: 416 53 83 399 24 2372 958 1753 Fragments arranged by size: 2372 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6058 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2035 2035 Size: 6058 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2384 2525 2609 4718 1239 Size: 1145 141 84 2109 2579 Fragments arranged by size: 2579 2109 1145 141 84 Bpu10I CC'TnA_GC Cuts at: 2271 3159 2271 Size: 888 5170 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3308 4183 5048 5346 3308 Size: 875 865 298 4020 Fragments arranged by size: 4020 875 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2402 3096 4721 882 Size: 617 903 694 1625 2219 Fragments arranged by size: 2219 1625 903 694 617 BsaAI yAC'GTr Cuts at: 493 1204 3849 493 Size: 711 2645 2702 Fragments arranged by size: 2702 2645 711 BsaBI GATnn'nnATC Cuts at: 6052 6052 Size: 6058 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2525 3865 4247 4882 Size: 53 83 186 936 992 1340 382 635 Cuts at: 4882 275 Size: 1451 Fragments arranged by size: 1451 1340 992 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4491 4889 5163 5310 5721 4491 Size: 398 274 147 411 4828 Fragments arranged by size: 4828 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5833 Fragments arranged by size: 5833 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2151 3317 1186 Size: 965 1166 3927 Fragments arranged by size: 3927 1166 965 BseYI C'CCAG_C Cuts at: 2064 5261 5757 2064 Size: 3197 496 2365 Fragments arranged by size: 3197 2365 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5485 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3508 4269 5297 1540 Size: 275 186 1507 761 1028 2301 Fragments arranged by size: 2301 1507 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2073 2393 2595 4119 4204 5275 2073 Size: 320 202 1524 85 1071 2856 Fragments arranged by size: 2856 1524 1071 320 202 85 BsmI GAATG_Cn' Cuts at: 3569 3662 3569 Size: 93 5965 BsmBI CGTCTCn'nnnn_ Cuts at: 2162 2477 2162 Size: 315 5743 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2073 2193 2325 2393 2556 2595 2746 Size: 399 283 120 132 68 163 39 151 Cuts at: 2746 2787 3047 3172 3377 4119 4204 5275 1391 Size: 41 260 125 205 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 283 260 205 163 151 132 125 120 85 68 41 39 BspEI T'CCGG_A Cuts at: 5721 5721 Size: 6058 BspHI T'CATG_A Cuts at: 3947 5677 3947 Size: 1730 4328 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3413 844 Size: 2569 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3945 1394 Size: 429 2122 3507 Fragments arranged by size: 3507 2122 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2062 2997 3153 4553 4727 65 Size: 1183 814 935 156 1400 174 1396 Fragments arranged by size: 1400 1396 1183 935 814 174 156 BsrFI r'CCGG_y Cuts at: 1700 1810 4702 4889 1700 Size: 110 2892 187 2869 Fragments arranged by size: 2892 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6058 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6058 BssSI C'ACGA_G Cuts at: 2354 4118 5130 2354 Size: 1764 1012 3282 Fragments arranged by size: 3282 1764 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2061 794 Size: 1267 4791 BstBI TT'CG_AA Cuts at: 4862 4862 Size: 6058 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6058 BstXI CCAn_nnnn'nTGG Cuts at: 2102 2426 2908 2102 Size: 324 482 5252 Fragments arranged by size: 5252 482 324 BstYI r'GATC_y Cuts at: 1972 3388 3755 4140 4157 5609 5695 5707 6053 Size: 1416 367 385 17 1452 86 12 346 Cuts at: 6053 1972 Size: 1977 Fragments arranged by size: 1977 1452 1416 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2929 2962 513 Size: 552 1864 33 3609 Fragments arranged by size: 3609 1864 552 33 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5319 Fragments arranged by size: 5319 683 56 BtsI GCAGTG_nn' Cuts at: 2205 2718 3574 4368 4388 2205 Size: 513 856 794 20 3875 Fragments arranged by size: 3875 856 794 513 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6058 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2209 2244 3136 3171 529 Size: 35 1645 35 892 35 3416 Fragments arranged by size: 3416 1645 892 35 35 35 DraI TTT'AAA Cuts at: 3362 3362 Size: 6058 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5065 809 Size: 507 3749 1802 Fragments arranged by size: 3749 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2032 2104 2379 Size: 54 1425 50 275 18 202 72 275 Cuts at: 2379 2811 3108 3505 4394 6023 8 Size: 432 297 397 889 1629 43 Fragments arranged by size: 1629 1425 889 432 397 297 275 275 202 72 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3505 1537 Size: 275 1693 4090 Fragments arranged by size: 4090 1693 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2267 3988 1353 Size: 914 1721 3423 Fragments arranged by size: 3423 1721 914 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4645 5019 5165 387 Size: 1064 138 3056 374 146 1280 Fragments arranged by size: 3056 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2071 2071 Size: 6058 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2146 2384 2525 2609 4121 4718 5505 Size: 393 514 238 141 84 1512 597 787 Cuts at: 5505 5773 1239 Size: 268 1524 Fragments arranged by size: 1524 1512 787 597 514 393 268 238 141 84 EcoNI CCTnn'n_nnAGG Cuts at: 3110 3290 3110 Size: 180 5878 EcoRI G'AATT_C Cuts at: 2367 2765 3367 3745 2367 Size: 398 602 378 4680 Fragments arranged by size: 4680 602 398 378 FspI TGC'GCA Cuts at: 4564 4564 Size: 6058 HaeII r_GCGC'y Cuts at: 1678 2528 5205 1678 Size: 850 2677 2531 Fragments arranged by size: 2677 2531 850 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4699 4731 4773 4805 1373 Size: 32 135 32 3127 32 42 32 2626 Fragments arranged by size: 3127 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2755 3402 3648 669 Size: 988 281 817 647 246 3079 Fragments arranged by size: 3079 988 817 647 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6058 HpaI GTT'AAC Cuts at: 3648 3648 Size: 6058 KasI G'GCGC_C Cuts at: 2524 2524 Size: 6058 KpnI G_GTAC'C Cuts at: 3383 3383 Size: 6058 MfeI C'AATT_G Cuts at: 3657 3657 Size: 6058 MluI A'CGCG_T Cuts at: 5934 5934 Size: 6058 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2340 2693 5172 5356 1305 Size: 1035 353 2479 184 2007 Fragments arranged by size: 2479 2007 1035 353 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2034 2106 2813 3110 10 Size: 54 1425 343 202 72 707 297 2958 Fragments arranged by size: 2958 1425 707 343 297 202 72 54 MslI CAynn'nnrTG Cuts at: 518 1166 3089 4016 4375 4534 518 Size: 648 1923 927 359 159 2042 Fragments arranged by size: 2042 1923 927 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2543 3474 4151 5299 5544 1606 Size: 937 931 677 1148 245 2120 Fragments arranged by size: 2120 1148 937 931 677 245 NarI GG'CG_CC Cuts at: 2525 2525 Size: 6058 NcoI C'CATG_G Cuts at: 513 1065 2929 513 Size: 552 1864 3642 Fragments arranged by size: 3642 1864 552 NdeI CA'TA_TG Cuts at: 387 2818 387 Size: 2431 3627 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6058 NotI GC'GGCC_GC Cuts at: 3505 3505 Size: 6058 NspI r_CATG'y Cuts at: 1132 1229 2189 3136 3416 4961 1132 Size: 97 960 947 280 1545 2229 Fragments arranged by size: 2229 1545 960 947 280 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6058 PciI A'CATG_T Cuts at: 4957 4957 Size: 6058 PflMI CCAn_nnn'nTGG Cuts at: 1108 3034 1108 Size: 1926 4132 PmeI GTTT'AAAC Cuts at: 3362 3362 Size: 6058 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4127 4159 5640 5672 4127 Size: 32 1481 32 4513 Fragments arranged by size: 4513 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2640 1613 Size: 1027 5031 PsiI TTA'TAA Cuts at: 3628 3628 Size: 6058 PstI C_TGCA'G Cuts at: 830 3410 830 Size: 2580 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6058 PvuII CAG'CTG Cuts at: 2543 2543 Size: 6058 SacI G_AGCT'C Cuts at: 2073 2073 Size: 6058 SalI G'TCGA_C Cuts at: 1936 3400 1936 Size: 1464 4594 SapI GCTCTTCn'nnn_ Cuts at: 2267 2267 Size: 6058 SbfI CC_TGCA'GG Cuts at: 3410 3410 Size: 6058 ScaI AGT'ACT Cuts at: 4306 6018 4306 Size: 1712 4346 SexAI A'CCwGG_T Cuts at: 2778 2778 Size: 6058 SfcI C'TryA_G Cuts at: 826 1045 2544 2865 3406 4541 5222 5413 826 Size: 219 1499 321 541 1135 681 191 1471 Fragments arranged by size: 1499 1471 1135 681 541 321 219 191 SfoI GGC'GCC Cuts at: 2526 2526 Size: 6058 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6058 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6058 SmaI CCC'GGG Cuts at: 1872 2195 3385 1872 Size: 323 1190 4545 Fragments arranged by size: 4545 1190 323 SmlI C'TyrA_G Cuts at: 820 1017 1943 3287 4162 5063 5325 820 Size: 197 926 1344 875 901 262 1553 Fragments arranged by size: 1553 1344 926 901 875 262 197 SnaBI TAC'GTA Cuts at: 493 3849 493 Size: 3356 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6058 SphI G_CATG'C Cuts at: 1132 3136 3416 1132 Size: 2004 280 3774 Fragments arranged by size: 3774 2004 280 SspI AAT'ATT Cuts at: 5 52 3982 5 Size: 47 3930 2081 Fragments arranged by size: 3930 2081 47 StuI AGG'CCT Cuts at: 1517 3164 1517 Size: 1647 4411 StyI C'CwwG_G Cuts at: 513 1065 2929 3111 3497 513 Size: 552 1864 182 386 3074 Fragments arranged by size: 3074 1864 552 386 182 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2379 2591 4085 4102 4255 4440 4885 Size: 1255 799 212 1494 17 153 185 445 Cuts at: 4885 325 Size: 1498 Fragments arranged by size: 1498 1494 1255 799 445 212 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4304 6016 96 Size: 274 80 33 51 1443 2327 1712 138 Fragments arranged by size: 2327 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6018 6050 6018 Size: 32 6026 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6058 XbaI T'CTAG_A Cuts at: 3394 3394 Size: 6058 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2426 3031 2426 Size: 605 5453 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6058 XmaI C'CCGG_G Cuts at: 1870 2193 3383 1870 Size: 323 1190 4545 Fragments arranged by size: 4545 1190 323 XmnI GAAnn'nnTTC Cuts at: 2440 4187 2440 Size: 1747 4311 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3866 276 Size: 53 83 186 936 2332 2468 Fragments arranged by size: 2468 2332 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BaeI BfrBI BsiWI BstZ17I Bsu36I DraIII EcoO109I EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SfiI SrfI SwaI