Restriction map for pFN21AE1483
 (Circular) (Six-base) MAPSORT of: pFN21AE1483.seq  Check: 2916  from: 1  to: 6058



pFN21AE1483  6058 bp

 With 182 enzymes: SgfI * 

                        August 16, 2010 14:52  ..

AatII G_ACGT'C

Cuts at:    278     331     414     600    1536    3868     278 
   Size:         53      83     186     936    2332    2468

  Fragments arranged by size: 

                2468    2332     936     186      83      53
 
AccI GT'mk_AC

Cuts at:   1937    2208    2616    3401    1937 
   Size:        271     408     785    4594

  Fragments arranged by size: 

                4594     785     408     271
 
Acc65I G'GTAC_C

Cuts at:   3379    3379 
   Size:       6058
 
AclI AA'CG_TT

Cuts at:   1563    4185    4558    1563 
   Size:       2622     373    3063

  Fragments arranged by size: 

                3063    2622     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1632    2146    4121    5505    5773    1632 
   Size:        514    1975    1384     268    1917

  Fragments arranged by size: 

                1975    1917    1384     514     268
 
AflII C'TTAA_G

Cuts at:    820    1017     820 
   Size:        197    5861
 
AflIII A'CryG_T

Cuts at:   1203    4957    5934    1203 
   Size:       3754     977    1327

  Fragments arranged by size: 

                3754    1327     977
 
AgeI A'CCGG_T

Cuts at:   4889    4889 
   Size:       6058
 
AhdI GACnn_n'nnGTC

Cuts at:   4787    4787 
   Size:       6058
 
AleI CACnn'nnGTG

Cuts at:   1166    3089    1166 
   Size:       1923    4135
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1184    2204    1184 
   Size:       1020    5038
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   2076    2108    2076 
   Size:         32    6026
 
AlwNI CAG_nnn'CTG

Cuts at:   3801    5373    3801 
   Size:       1572    4486
 
ApaLI G'TGCA_C

Cuts at:   2389    4115    5271    2389 
   Size:       1726    1156    3176

  Fragments arranged by size: 

                3176    1726    1156
 
ApoI r'AATT_y

Cuts at:   1493    2367    2765    3367    3593    3745    5854    1493 
   Size:        874     398     602     226     152    2109    1697

  Fragments arranged by size: 

                2109    1697     874     602     398     226     152
 
AseI AT'TA_AT

Cuts at:    160    1032    4612     160 
   Size:        872    3580    1606

  Fragments arranged by size: 

                3580    1606     872
 
AsiSI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       6058
 
AvaI C'yCGr_G

Cuts at:   1870    1943    2193    2316    3383    1870 
   Size:         73     250     123    1067    4545

  Fragments arranged by size: 

                4545    1067     250     123      73
 
BamHI G'GATC_C

Cuts at:   3388    3755    3388 
   Size:        367    5691
 
BanI G'GyrC_C

Cuts at:    618     943    1160    1787    2246    2524    2782    3042    3091 
   Size:        325     217     627     459     278     258     260      49

Cuts at:   3091    3379    4834     618 
   Size:        288    1455    1842

  Fragments arranged by size: 

                1842    1455     627     459     325     288     278     260
                 258     217      49
 
BanII G_rGCy'C

Cuts at:   1391    2073    2556    2746    3172    3377    1391 
   Size:        682     483     190     426     205    4072

  Fragments arranged by size: 

                4072     682     483     426     205     190
 
BbeI G_GCGC'C

Cuts at:   2528    2528 
   Size:       6058
 
BbsI GAAGACnn'nnnn_

Cuts at:    928    1899     928 
   Size:        971    5087
 
BbvCI CC'TCA_GC

Cuts at:   3159    3159 
   Size:       6058
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1903    1937    1987    2021    4249    4283    1903 
   Size:         34      50      34    2228      34    3678

  Fragments arranged by size: 

                3678    2228      50      34      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   3950    5160    3950 
   Size:       1210    4848
 
BclI T'GATC_A

Cuts at:   1548    1548 
   Size:       6058
 
BglI GCCn_nnn'nGGC

Cuts at:    136     243     365     436    1067    1672    3051    4669     136 
   Size:        107     122      71     631     605    1379    1618    1525

  Fragments arranged by size: 

                1618    1525    1379     631     605     122     107      71
            
 
BglII A'GATC_T

Cuts at:   6053    6053 
   Size:       6058
 
BlpI GC'TnA_GC

Cuts at:   3137    3475    3137 
   Size:        338    5720
 
Bme1580I G_kGCm'C

Cuts at:   1790    2193    2325    2393    2787    3047    4119    5275    1790 
   Size:        403     132      68     394     260    1072    1156    2573

  Fragments arranged by size: 

                2573    1156    1072     403     394     260     132      68
            
 
BmgBI CAC'GTC

Cuts at:   1318    2971    1318 
   Size:       1653    4405
 
BmrI ACTGGGnnnn_n'

Cuts at:    442     858     911     994    1393    1417    3789    4747     442 
   Size:        416      53      83     399      24    2372     958    1753

  Fragments arranged by size: 

                2372    1753     958     416     399      83      53      24
            
 
BmtI G_CTAG'C

Cuts at:   1056    1056 
   Size:       6058
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2035    2035 
   Size:       6058
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    2384    2525    2609    4718    1239 
   Size:       1145     141      84    2109    2579

  Fragments arranged by size: 

                2579    2109    1145     141      84
 
Bpu10I CC'TnA_GC

Cuts at:   2271    3159    2271 
   Size:        888    5170
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   3308    4183    5048    5346    3308 
   Size:        875     865     298    4020

  Fragments arranged by size: 

                4020     875     865     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    882    1499    2402    3096    4721     882 
   Size:        617     903     694    1625    2219

  Fragments arranged by size: 

                2219    1625     903     694     617
 
BsaAI yAC'GTr

Cuts at:    493    1204    3849     493 
   Size:        711    2645    2702

  Fragments arranged by size: 

                2702    2645     711
 
BsaBI GATnn'nnATC

Cuts at:   6052    6052 
   Size:       6058
 
BsaHI Gr'CG_yC

Cuts at:    275     328     411     597    1533    2525    3865    4247    4882 
   Size:         53      83     186     936     992    1340     382     635

Cuts at:   4882     275 
   Size:       1451

  Fragments arranged by size: 

                1451    1340     992     936     635     382     186      83
                  53
 
BsaWI w'CCGG_w

Cuts at:   4491    4889    5163    5310    5721    4491 
   Size:        398     274     147     411    4828

  Fragments arranged by size: 

                4828     411     398     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1178    1208    1373    1403    1178 
   Size:         30     165      30    5833

  Fragments arranged by size: 

                5833     165      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1186    2151    3317    1186 
   Size:        965    1166    3927

  Fragments arranged by size: 

                3927    1166     965
 
BseYI C'CCAG_C

Cuts at:   2064    5261    5757    2064 
   Size:       3197     496    2365

  Fragments arranged by size: 

                3197    2365     496
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1736    1163 
   Size:        573    5485
 
BsiEI CG_ry'CG

Cuts at:   1540    1815    2001    3508    4269    5297    1540 
   Size:        275     186    1507     761    1028    2301

  Fragments arranged by size: 

                2301    1507    1028     761     275     186
 
BsiHKAI G_wGCw'C

Cuts at:   2073    2393    2595    4119    4204    5275    2073 
   Size:        320     202    1524      85    1071    2856

  Fragments arranged by size: 

                2856    1524    1071     320     202      85
 
BsmI GAATG_Cn'

Cuts at:   3569    3662    3569 
   Size:         93    5965
 
BsmBI CGTCTCn'nnnn_

Cuts at:   2162    2477    2162 
   Size:        315    5743
 
Bsp1286I G_dGCh'C

Cuts at:   1391    1790    2073    2193    2325    2393    2556    2595    2746 
   Size:        399     283     120     132      68     163      39     151

Cuts at:   2746    2787    3047    3172    3377    4119    4204    5275    1391 
   Size:         41     260     125     205     742      85    1071    2174

  Fragments arranged by size: 

                2174    1071     742     399     283     260     205     163
                 151     132     125     120      85      68      41      39
            
 
BspEI T'CCGG_A

Cuts at:   5721    5721 
   Size:       6058
 
BspHI T'CATG_A

Cuts at:   3947    5677    3947 
   Size:       1730    4328
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    844    3413     844 
   Size:       2569    3489
 
BsrBI CCG'CTC

Cuts at:   1394    1823    3945    1394 
   Size:        429    2122    3507

  Fragments arranged by size: 

                3507    2122     429
 
BsrDI GCAATG_nn'

Cuts at:     65    1248    2062    2997    3153    4553    4727      65 
   Size:       1183     814     935     156    1400     174    1396

  Fragments arranged by size: 

                1400    1396    1183     935     814     174     156
 
BsrFI r'CCGG_y

Cuts at:   1700    1810    4702    4889    1700 
   Size:        110    2892     187    2869

  Fragments arranged by size: 

                2892    2869     187     110
 
BsrGI T'GTAC_A

Cuts at:     96      96 
   Size:       6058
 
BssHII G'CGCG_C

Cuts at:   1925    1925 
   Size:       6058
 
BssSI C'ACGA_G

Cuts at:   2354    4118    5130    2354 
   Size:       1764    1012    3282

  Fragments arranged by size: 

                3282    1764    1012
 
BstAPI GCAn_nnn'nTGC

Cuts at:    794    2061     794 
   Size:       1267    4791
 
BstBI TT'CG_AA

Cuts at:   4862    4862 
   Size:       6058
 
BstEII G'GTnAC_C

Cuts at:   1184    1184 
   Size:       6058
 
BstXI CCAn_nnnn'nTGG

Cuts at:   2102    2426    2908    2102 
   Size:        324     482    5252

  Fragments arranged by size: 

                5252     482     324
 
BstYI r'GATC_y

Cuts at:   1972    3388    3755    4140    4157    5609    5695    5707    6053 
   Size:       1416     367     385      17    1452      86      12     346

Cuts at:   6053    1972 
   Size:       1977

  Fragments arranged by size: 

                1977    1452    1416     385     367     346      86      17
                  12
 
BtgI C'CryG_G

Cuts at:    513    1065    2929    2962     513 
   Size:        552    1864      33    3609

  Fragments arranged by size: 

                3609    1864     552      33
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    487    1170    1226     487 
   Size:        683      56    5319

  Fragments arranged by size: 

                5319     683      56
 
BtsI GCAGTG_nn'

Cuts at:   2205    2718    3574    4368    4388    2205 
   Size:        513     856     794      20    3875

  Fragments arranged by size: 

                3875     856     794     513      20
 
ClaI AT'CG_AT

Cuts at:   1554    1554 
   Size:       6058
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    529     564    2209    2244    3136    3171     529 
   Size:         35    1645      35     892      35    3416

  Fragments arranged by size: 

                3416    1645     892      35      35      35
 
DraI TTT'AAA

Cuts at:   3362    3362 
   Size:       6058
 
DrdI GACnn_nn'nnGTC

Cuts at:    809    1316    5065     809 
   Size:        507    3749    1802

  Fragments arranged by size: 

                3749    1802     507
 
EaeI y'GGCC_r

Cuts at:      8      62    1487    1537    1812    1830    2032    2104    2379 
   Size:         54    1425      50     275      18     202      72     275

Cuts at:   2379    2811    3108    3505    4394    6023       8 
   Size:        432     297     397     889    1629      43

  Fragments arranged by size: 

                1629    1425     889     432     397     297     275     275
                 202      72      54      50      43      18
 
EagI C'GGCC_G

Cuts at:   1537    1812    3505    1537 
   Size:        275    1693    4090

  Fragments arranged by size: 

                4090    1693     275
 
EarI CTCTTCn'nnn_

Cuts at:   1353    2267    3988    1353 
   Size:        914    1721    3423

  Fragments arranged by size: 

                3423    1721     914
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    387    1451    1589    4645    5019    5165     387 
   Size:       1064     138    3056     374     146    1280

  Fragments arranged by size: 

                3056    1280    1064     374     146     138
 
EcoICRI GAG'CTC

Cuts at:   2071    2071 
   Size:       6058
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    1632    2146    2384    2525    2609    4121    4718    5505 
   Size:        393     514     238     141      84    1512     597     787

Cuts at:   5505    5773    1239 
   Size:        268    1524

  Fragments arranged by size: 

                1524    1512     787     597     514     393     268     238
                 141      84
 
EcoNI CCTnn'n_nnAGG

Cuts at:   3110    3290    3110 
   Size:        180    5878
 
EcoRI G'AATT_C

Cuts at:   2367    2765    3367    3745    2367 
   Size:        398     602     378    4680

  Fragments arranged by size: 

                4680     602     398     378
 
FspI TGC'GCA

Cuts at:   4564    4564 
   Size:       6058
 
HaeII r_GCGC'y

Cuts at:   1678    2528    5205    1678 
   Size:        850    2677    2531

  Fragments arranged by size: 

                2677    2531     850
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1373    1405    1540    1572    4699    4731    4773    4805    1373 
   Size:         32     135      32    3127      32      42      32    2626

  Fragments arranged by size: 

                3127    2626     135      42      32      32      32      32
            
 
HincII GTy'rAC

Cuts at:    669    1657    1938    2755    3402    3648     669 
   Size:        988     281     817     647     246    3079

  Fragments arranged by size: 

                3079     988     817     647     281     246
 
HindIII A'AGCT_T

Cuts at:    748     748 
   Size:       6058
 
HpaI GTT'AAC

Cuts at:   3648    3648 
   Size:       6058
 
KasI G'GCGC_C

Cuts at:   2524    2524 
   Size:       6058
 
KpnI G_GTAC'C

Cuts at:   3383    3383 
   Size:       6058
 
MfeI C'AATT_G

Cuts at:   3657    3657 
   Size:       6058
 
MluI A'CGCG_T

Cuts at:   5934    5934 
   Size:       6058
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1305    2340    2693    5172    5356    1305 
   Size:       1035     353    2479     184    2007

  Fragments arranged by size: 

                2479    2007    1035     353     184
 
MscI TGG'CCA

Cuts at:     10      64    1489    1832    2034    2106    2813    3110      10 
   Size:         54    1425     343     202      72     707     297    2958

  Fragments arranged by size: 

                2958    1425     707     343     297     202      72      54
            
 
MslI CAynn'nnrTG

Cuts at:    518    1166    3089    4016    4375    4534     518 
   Size:        648    1923     927     359     159    2042

  Fragments arranged by size: 

                2042    1923     927     648     359     159
 
MspA1I CmG'CkG

Cuts at:   1606    2543    3474    4151    5299    5544    1606 
   Size:        937     931     677    1148     245    2120

  Fragments arranged by size: 

                2120    1148     937     931     677     245
 
NarI GG'CG_CC

Cuts at:   2525    2525 
   Size:       6058
 
NcoI C'CATG_G

Cuts at:    513    1065    2929     513 
   Size:        552    1864    3642

  Fragments arranged by size: 

                3642    1864     552
 
NdeI CA'TA_TG

Cuts at:    387    2818     387 
   Size:       2431    3627
 
NheI G'CTAG_C

Cuts at:   1052    1052 
   Size:       6058
 
NotI GC'GGCC_GC

Cuts at:   3505    3505 
   Size:       6058
 
NspI r_CATG'y

Cuts at:   1132    1229    2189    3136    3416    4961    1132 
   Size:         97     960     947     280    1545    2229

  Fragments arranged by size: 

                2229    1545     960     947     280      97
 
PasI CC'CwG_GG

Cuts at:   1348    1348 
   Size:       6058
 
PciI A'CATG_T

Cuts at:   4957    4957 
   Size:       6058
 
PflMI CCAn_nnn'nTGG

Cuts at:   1108    3034    1108 
   Size:       1926    4132
 
PmeI GTTT'AAAC

Cuts at:   3362    3362 
   Size:       6058
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   4127    4159    5640    5672    4127 
   Size:         32    1481      32    4513

  Fragments arranged by size: 

                4513    1481      32      32
 
PshAI GACnn'nnGTC

Cuts at:   1613    2640    1613 
   Size:       1027    5031
 
PsiI TTA'TAA

Cuts at:   3628    3628 
   Size:       6058
 
PstI C_TGCA'G

Cuts at:    830    3410     830 
   Size:       2580    3478
 
PvuI CG_AT'CG

Cuts at:   2001    2001 
   Size:       6058
 
PvuII CAG'CTG

Cuts at:   2543    2543 
   Size:       6058
 
SacI G_AGCT'C

Cuts at:   2073    2073 
   Size:       6058
 
SalI G'TCGA_C

Cuts at:   1936    3400    1936 
   Size:       1464    4594
 
SapI GCTCTTCn'nnn_

Cuts at:   2267    2267 
   Size:       6058
 
SbfI CC_TGCA'GG

Cuts at:   3410    3410 
   Size:       6058
 
ScaI AGT'ACT

Cuts at:   4306    6018    4306 
   Size:       1712    4346
 
SexAI A'CCwGG_T

Cuts at:   2778    2778 
   Size:       6058
 
SfcI C'TryA_G

Cuts at:    826    1045    2544    2865    3406    4541    5222    5413     826 
   Size:        219    1499     321     541    1135     681     191    1471

  Fragments arranged by size: 

                1499    1471    1135     681     541     321     219     191
            
 
SfoI GGC'GCC

Cuts at:   2526    2526 
   Size:       6058
 
SgfI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       6058
 
SgrAI Cr'CCGG_yG

Cuts at:   1700    1700 
   Size:       6058
 
SmaI CCC'GGG

Cuts at:   1872    2195    3385    1872 
   Size:        323    1190    4545

  Fragments arranged by size: 

                4545    1190     323
 
SmlI C'TyrA_G

Cuts at:    820    1017    1943    3287    4162    5063    5325     820 
   Size:        197     926    1344     875     901     262    1553

  Fragments arranged by size: 

                1553    1344     926     901     875     262     197
 
SnaBI TAC'GTA

Cuts at:    493    3849     493 
   Size:       3356    2702
 
SpeI A'CTAG_T

Cuts at:    152     152 
   Size:       6058
 
SphI G_CATG'C

Cuts at:   1132    3136    3416    1132 
   Size:       2004     280    3774

  Fragments arranged by size: 

                3774    2004     280
 
SspI AAT'ATT

Cuts at:      5      52    3982       5 
   Size:         47    3930    2081

  Fragments arranged by size: 

                3930    2081      47
 
StuI AGG'CCT

Cuts at:   1517    3164    1517 
   Size:       1647    4411
 
StyI C'CwwG_G

Cuts at:    513    1065    2929    3111    3497     513 
   Size:        552    1864     182     386    3074

  Fragments arranged by size: 

                3074    1864     552     386     182
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    325    1580    2379    2591    4085    4102    4255    4440    4885 
   Size:       1255     799     212    1494      17     153     185     445

Cuts at:   4885     325 
   Size:       1498

  Fragments arranged by size: 

                1498    1494    1255     799     445     212     185     153
                  17
 
TatI w'GTAC_w

Cuts at:     96     370     450     483     534    1977    4304    6016      96 
   Size:        274      80      33      51    1443    2327    1712     138

  Fragments arranged by size: 

                2327    1712    1443     274     138      80      51      33
            
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   6018    6050    6018 
   Size:         32    6026
 
Tth111I GACn'n_nGTC

Cuts at:   1316    1316 
   Size:       6058
 
XbaI T'CTAG_A

Cuts at:   3394    3394 
   Size:       6058
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   2426    3031    2426 
   Size:        605    5453
 
XhoI C'TCGA_G

Cuts at:   1943    1943 
   Size:       6058
 
XmaI C'CCGG_G

Cuts at:   1870    2193    3383    1870 
   Size:        323    1190    4545

  Fragments arranged by size: 

                4545    1190     323
 
XmnI GAAnn'nnTTC

Cuts at:   2440    4187    2440 
   Size:       1747    4311
 
ZraI GAC'GTC

Cuts at:    276     329     412     598    1534    3866     276 
   Size:         53      83     186     936    2332    2468

  Fragments arranged by size: 

                2468    2332     936     186      83      53
 

 Enzymes that do cut:

    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI    ApaLI     ApoI     AseI
    AsiSI     AvaI    BamHI     BanI    BanII     BbeI     BbsI    BbvCI
     BcgI    BciVI     BclI     BglI    BglII     BlpI Bme1580I    BmgBI
     BmrI     BmtI     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI
    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI
  BsiHKAI     BsmI    BsmBI Bsp1286I    BspEI    BspHI    BspMI    BsrBI
    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII
    BstXI    BstYI     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI
     DrdI     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI    EcoNI
    EcoRI     FspI    HaeII    Hin4I   HincII  HindIII     HpaI     KasI
     KpnI     MfeI     MluI     MmeI     MscI     MslI   MspA1I     NarI
     NcoI     NdeI     NheI     NotI     NspI     PasI     PciI    PflMI
     PmeI     PpiI    PshAI     PsiI     PstI     PvuI    PvuII     SacI
     SalI     SapI     SbfI     ScaI    SexAI     SfcI     SfoI     SgfI
    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SspI     StuI
     StyI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XhoI
     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AarI     AfeI     ApaI     AscI    AvrII     BaeI    BfrBI    BsiWI
  BstZ17I   Bsu36I   DraIII EcoO109I    EcoRV     FalI     FseI    FspAI
     NaeI   NgoMIV     NruI     NsiI     PacI     PfoI     PmlI    PpuMI
   PspOMI    PspXI     PsrI    RsrII    SacII    SanDI     SfiI     SrfI
     SwaI
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