(Linear) (Six-base) MAPSORT of: KIEE1483.seq Check: 3001 from: 1 to: 1353 KIEE1483.orf With 182 enzymes: SgfI * July 15, 2010 18:40 .. AccI GT'mk_AC Cuts at: 0 203 611 1353 Size: 203 408 742 Fragments arranged by size: 742 408 203 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 141 1353 Size: 141 1212 AleI CACnn'nnGTG Cuts at: 0 1084 1353 Size: 1084 269 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 199 1353 Size: 199 1154 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 71 103 1353 Size: 71 32 1250 Fragments arranged by size: 1250 71 32 ApaLI G'TGCA_C Cuts at: 0 384 1353 Size: 384 969 ApoI r'AATT_y Cuts at: 0 362 760 1353 Size: 362 398 593 Fragments arranged by size: 593 398 362 AvaI C'yCGr_G Cuts at: 0 188 311 1353 Size: 188 123 1042 Fragments arranged by size: 1042 188 123 BanI G'GyrC_C Cuts at: 0 241 519 777 1037 1086 1353 Size: 241 278 258 260 49 267 Fragments arranged by size: 278 267 260 258 241 49 BanII G_rGCy'C Cuts at: 0 68 551 741 1167 1353 Size: 68 483 190 426 186 Fragments arranged by size: 483 426 190 186 68 BbeI G_GCGC'C Cuts at: 0 523 1353 Size: 523 830 BbvCI CC'TCA_GC Cuts at: 0 1154 1353 Size: 1154 199 BglI GCCn_nnn'nGGC Cuts at: 0 1046 1353 Size: 1046 307 BlpI GC'TnA_GC Cuts at: 0 1132 1353 Size: 1132 221 Bme1580I G_kGCm'C Cuts at: 0 188 320 388 782 1042 1353 Size: 188 132 68 394 260 311 Fragments arranged by size: 394 311 260 188 132 68 BmgBI CAC'GTC Cuts at: 0 966 1353 Size: 966 387 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 30 1353 Size: 30 1323 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 379 520 604 1353 Size: 379 141 84 749 Fragments arranged by size: 749 379 141 84 Bpu10I CC'TnA_GC Cuts at: 0 266 1154 1353 Size: 266 888 199 Fragments arranged by size: 888 266 199 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1303 1353 Size: 1303 50 BsaI GGTCTCn'nnnn_ Cuts at: 0 397 1091 1353 Size: 397 694 262 Fragments arranged by size: 694 397 262 BsaHI Gr'CG_yC Cuts at: 0 520 1353 Size: 520 833 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 146 1312 1353 Size: 146 1166 41 Fragments arranged by size: 1166 146 41 BseYI C'CCAG_C Cuts at: 0 59 1353 Size: 59 1294 BsiHKAI G_wGCw'C Cuts at: 0 68 388 590 1353 Size: 68 320 202 763 Fragments arranged by size: 763 320 202 68 BsmBI CGTCTCn'nnnn_ Cuts at: 0 157 472 1353 Size: 157 315 881 Fragments arranged by size: 881 315 157 Bsp1286I G_dGCh'C Cuts at: 0 68 188 320 388 551 590 741 782 Size: 68 120 132 68 163 39 151 41 Cuts at: 782 1042 1167 1353 Size: 260 125 186 Fragments arranged by size: 260 186 163 151 132 125 120 68 68 41 39 BsrDI GCAATG_nn' Cuts at: 0 57 992 1148 1353 Size: 57 935 156 205 Fragments arranged by size: 935 205 156 57 BssSI C'ACGA_G Cuts at: 0 349 1353 Size: 349 1004 BstAPI GCAn_nnn'nTGC Cuts at: 0 56 1353 Size: 56 1297 BstXI CCAn_nnnn'nTGG Cuts at: 0 97 421 903 1353 Size: 97 324 482 450 Fragments arranged by size: 482 450 324 97 BtgI C'CryG_G Cuts at: 0 924 957 1353 Size: 924 33 396 Fragments arranged by size: 924 396 33 BtsI GCAGTG_nn' Cuts at: 0 200 713 1353 Size: 200 513 640 Fragments arranged by size: 640 513 200 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 204 239 1131 1166 1353 Size: 204 35 892 35 187 Fragments arranged by size: 892 204 187 35 35 EaeI y'GGCC_r Cuts at: 0 27 99 374 806 1103 1353 Size: 27 72 275 432 297 250 Fragments arranged by size: 432 297 275 250 72 27 EarI CTCTTCn'nnn_ Cuts at: 0 262 1353 Size: 262 1091 EcoICRI GAG'CTC Cuts at: 0 66 1353 Size: 66 1287 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 141 379 520 604 1353 Size: 141 238 141 84 749 Fragments arranged by size: 749 238 141 141 84 EcoNI CCTnn'n_nnAGG Cuts at: 0 1105 1285 1353 Size: 1105 180 68 Fragments arranged by size: 1105 180 68 EcoRI G'AATT_C Cuts at: 0 362 760 1353 Size: 362 398 593 Fragments arranged by size: 593 398 362 HaeII r_GCGC'y Cuts at: 0 523 1353 Size: 523 830 HincII GTy'rAC Cuts at: 0 750 1353 Size: 750 603 KasI G'GCGC_C Cuts at: 0 519 1353 Size: 519 834 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 335 688 1353 Size: 335 353 665 Fragments arranged by size: 665 353 335 MscI TGG'CCA Cuts at: 0 29 101 808 1105 1353 Size: 29 72 707 297 248 Fragments arranged by size: 707 297 248 72 29 MslI CAynn'nnrTG Cuts at: 0 1084 1353 Size: 1084 269 MspA1I CmG'CkG Cuts at: 0 538 1353 Size: 538 815 NarI GG'CG_CC Cuts at: 0 520 1353 Size: 520 833 NcoI C'CATG_G Cuts at: 0 924 1353 Size: 924 429 NdeI CA'TA_TG Cuts at: 0 813 1353 Size: 813 540 NspI r_CATG'y Cuts at: 0 184 1131 1353 Size: 184 947 222 Fragments arranged by size: 947 222 184 PflMI CCAn_nnn'nTGG Cuts at: 0 1029 1353 Size: 1029 324 PshAI GACnn'nnGTC Cuts at: 0 635 1353 Size: 635 718 PvuII CAG'CTG Cuts at: 0 538 1353 Size: 538 815 SacI G_AGCT'C Cuts at: 0 68 1353 Size: 68 1285 SapI GCTCTTCn'nnn_ Cuts at: 0 262 1353 Size: 262 1091 SexAI A'CCwGG_T Cuts at: 0 773 1353 Size: 773 580 SfcI C'TryA_G Cuts at: 0 539 860 1353 Size: 539 321 493 Fragments arranged by size: 539 493 321 SfoI GGC'GCC Cuts at: 0 521 1353 Size: 521 832 SmaI CCC'GGG Cuts at: 0 190 1353 Size: 190 1163 SmlI C'TyrA_G Cuts at: 0 1282 1353 Size: 1282 71 SphI G_CATG'C Cuts at: 0 1131 1353 Size: 1131 222 StuI AGG'CCT Cuts at: 0 1159 1353 Size: 1159 194 StyI C'CwwG_G Cuts at: 0 924 1106 1353 Size: 924 182 247 Fragments arranged by size: 924 247 182 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 374 586 1353 Size: 374 212 767 Fragments arranged by size: 767 374 212 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 421 1026 1353 Size: 421 605 327 Fragments arranged by size: 605 421 327 XmaI C'CCGG_G Cuts at: 0 188 1353 Size: 188 1165 XmnI GAAnn'nnTTC Cuts at: 0 435 1353 Size: 435 918 Enzymes that do cut: AccI AcuI AleI AlfI AloI ApaLI ApoI AvaI BanI BanII BbeI BbvCI BglI BlpI Bme1580I BmgBI BplI BpmI Bpu10I BpuEI BsaI BsaHI BseRI BseYI BsiHKAI BsmBI Bsp1286I BsrDI BssSI BstAPI BstXI BtgI BtsI CspCI EaeI EarI EcoICRI Eco57MI EcoNI EcoRI HaeII HincII KasI MmeI MscI MslI MspA1I NarI NcoI NdeI NspI PflMI PshAI PvuII SacI SapI SexAI SfcI SfoI SmaI SmlI SphI StuI StyI TaqII XcmI XmaI XmnI Enzymes that do not cut: AarI AatII Acc65I AclI AfeI AflII AflIII AgeI AhdI AlwNI ApaI AscI AseI AsiSI AvrII BaeI BamHI BbsI BcgI BciVI BclI BfrBI BglII BmrI BmtI BsaAI BsaBI BsaWI BsaXI BsgI BsiEI BsiWI BsmI BspEI BspHI BspMI BsrBI BsrFI BsrGI BssHII BstBI BstEII BstYI BstZ17I Bsu36I BtgZI ClaI DraI DraIII DrdI EagI EciI EcoO109I EcoRV FalI FseI FspI FspAI Hin4I HindIII HpaI KpnI MfeI MluI NaeI NgoMIV NheI NotI NruI NsiI PacI PasI PciI PfoI PmeI PmlI PpiI PpuMI PsiI PspOMI PspXI PsrI PstI PvuI RsrII SacII SalI SanDI SbfI ScaI SfiI SgfI SgrAI SnaBI SpeI SrfI SspI SwaI TatI TstI Tth111I XbaI XhoI ZraI