(Linear) (Six-base) MAPSORT of: KIEE6433.seq Check: 623 from: 1 to: 1341 KIEE6433.orf With 182 enzymes: SgfI * March 16, 2015 11:31 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 510 1341 Size: 510 831 AccI GT'mk_AC Cuts at: 0 469 1158 1341 Size: 469 689 183 Fragments arranged by size: 689 469 183 Acc65I G'GTAC_C Cuts at: 0 269 1341 Size: 269 1072 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 853 1341 Size: 853 488 AflIII A'CryG_T Cuts at: 0 206 1214 1341 Size: 206 1008 127 Fragments arranged by size: 1008 206 127 AgeI A'CCGG_T Cuts at: 0 272 1341 Size: 272 1069 AhdI GACnn_n'nnGTC Cuts at: 0 465 1341 Size: 465 876 AlwNI CAG_nnn'CTG Cuts at: 0 309 990 1341 Size: 309 681 351 Fragments arranged by size: 681 351 309 ApaI G_GGCC'C Cuts at: 0 342 1341 Size: 342 999 ApaLI G'TGCA_C Cuts at: 0 999 1341 Size: 999 342 ApoI r'AATT_y Cuts at: 0 501 676 737 1078 1103 1341 Size: 501 175 61 341 25 238 Fragments arranged by size: 501 341 238 175 61 25 AvaI C'yCGr_G Cuts at: 0 244 344 1341 Size: 244 100 997 Fragments arranged by size: 997 244 100 AvrII C'CTAG_G Cuts at: 0 1108 1341 Size: 1108 233 BanI G'GyrC_C Cuts at: 0 145 269 331 1341 Size: 145 124 62 1010 Fragments arranged by size: 1010 145 124 62 BanII G_rGCy'C Cuts at: 0 342 1341 Size: 342 999 BbsI GAAGACnn'nnnn_ Cuts at: 0 804 1063 1338 1341 Size: 804 259 275 3 Fragments arranged by size: 804 275 259 3 BciVI GTATCCnnnnn_n' Cuts at: 0 653 1341 Size: 653 688 BclI T'GATC_A Cuts at: 0 300 1341 Size: 300 1041 BfrBI ATG'CAT Cuts at: 0 1140 1341 Size: 1140 201 Bme1580I G_kGCm'C Cuts at: 0 342 850 1003 1179 1341 Size: 342 508 153 176 162 Fragments arranged by size: 508 342 176 162 153 BmrI ACTGGGnnnn_n' Cuts at: 0 1044 1341 Size: 1044 297 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 93 1145 1341 Size: 93 1052 196 Fragments arranged by size: 1052 196 93 BsaI GGTCTCn'nnnn_ Cuts at: 0 204 587 1341 Size: 204 383 754 Fragments arranged by size: 754 383 204 BsaHI Gr'CG_yC Cuts at: 0 128 1341 Size: 128 1213 BsaWI w'CCGG_w Cuts at: 0 272 510 1341 Size: 272 238 831 Fragments arranged by size: 831 272 238 BseYI C'CCAG_C Cuts at: 0 239 1341 Size: 239 1102 BsiEI CG_ry'CG Cuts at: 0 156 473 1341 Size: 156 317 868 Fragments arranged by size: 868 317 156 BsiHKAI G_wGCw'C Cuts at: 0 950 1003 1341 Size: 950 53 338 Fragments arranged by size: 950 338 53 BsmI GAATG_Cn' Cuts at: 0 539 612 981 1341 Size: 539 73 369 360 Fragments arranged by size: 539 369 360 73 Bsp1286I G_dGCh'C Cuts at: 0 342 850 950 1003 1179 1341 Size: 342 508 100 53 176 162 Fragments arranged by size: 508 342 176 162 100 53 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 510 605 1341 Size: 510 95 736 Fragments arranged by size: 736 510 95 BsrDI GCAATG_nn' Cuts at: 0 87 1341 Size: 87 1254 BsrFI r'CCGG_y Cuts at: 0 148 272 377 1341 Size: 148 124 105 964 Fragments arranged by size: 964 148 124 105 BssSI C'ACGA_G Cuts at: 0 43 1341 Size: 43 1298 BstBI TT'CG_AA Cuts at: 0 414 1341 Size: 414 927 BstXI CCAn_nnnn'nTGG Cuts at: 0 1082 1341 Size: 1082 259 BstYI r'GATC_y Cuts at: 0 1112 1341 Size: 1112 229 Bsu36I CC'TnA_GG Cuts at: 0 310 1341 Size: 310 1031 BtgI C'CryG_G Cuts at: 0 156 1341 Size: 156 1185 EaeI y'GGCC_r Cuts at: 0 50 153 657 1341 Size: 50 103 504 684 Fragments arranged by size: 684 504 103 50 EagI C'GGCC_G Cuts at: 0 153 1341 Size: 153 1188 EarI CTCTTCn'nnn_ Cuts at: 0 770 1052 1341 Size: 770 282 289 Fragments arranged by size: 770 289 282 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 265 1341 Size: 265 1076 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 93 853 1145 1341 Size: 93 760 292 196 Fragments arranged by size: 760 292 196 93 EcoNI CCTnn'n_nnAGG Cuts at: 0 775 1341 Size: 775 566 EcoO109I rG'GnC_Cy Cuts at: 0 105 339 875 1341 Size: 105 234 536 466 Fragments arranged by size: 536 466 234 105 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 157 189 451 483 678 710 850 882 Size: 157 32 262 32 195 32 140 32 Cuts at: 882 1341 Size: 459 Fragments arranged by size: 459 262 195 157 140 32 32 32 32 HincII GTy'rAC Cuts at: 0 470 1341 Size: 470 871 KpnI G_GTAC'C Cuts at: 0 273 1341 Size: 273 1068 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 227 1341 Size: 227 1114 MspA1I CmG'CkG Cuts at: 0 158 291 486 1341 Size: 158 133 195 855 Fragments arranged by size: 855 195 158 133 NaeI GCC'GGC Cuts at: 0 150 1341 Size: 150 1191 NgoMIV G'CCGG_C Cuts at: 0 148 1341 Size: 148 1193 NotI GC'GGCC_GC Cuts at: 0 153 1341 Size: 153 1188 NsiI A_TGCA'T Cuts at: 0 1142 1341 Size: 1142 199 NspI r_CATG'y Cuts at: 0 1218 1341 Size: 1218 123 PasI CC'CwG_GG Cuts at: 0 423 870 1341 Size: 423 447 471 Fragments arranged by size: 471 447 423 PciI A'CATG_T Cuts at: 0 1214 1341 Size: 1214 127 PpuMI rG'GwC_Cy Cuts at: 0 875 1341 Size: 875 466 PsiI TTA'TAA Cuts at: 0 1031 1341 Size: 1031 310 PspOMI G'GGCC_C Cuts at: 0 338 1341 Size: 338 1003 PstI C_TGCA'G Cuts at: 0 521 1340 1341 Size: 521 819 1 Fragments arranged by size: 819 521 1 PvuII CAG'CTG Cuts at: 0 291 486 1341 Size: 291 195 855 Fragments arranged by size: 855 291 195 SacII CC_GC'GG Cuts at: 0 159 1341 Size: 159 1182 SalI G'TCGA_C Cuts at: 0 468 1341 Size: 468 873 SfcI C'TryA_G Cuts at: 0 517 879 1155 1336 1341 Size: 517 362 276 181 5 Fragments arranged by size: 517 362 276 181 5 SmaI CCC'GGG Cuts at: 0 346 1341 Size: 346 995 StyI C'CwwG_G Cuts at: 0 1108 1341 Size: 1108 233 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 487 1341 Size: 487 854 TatI w'GTAC_w Cuts at: 0 1015 1341 Size: 1015 326 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 867 899 1341 Size: 867 32 442 Fragments arranged by size: 867 442 32 Tth111I GACn'n_nGTC Cuts at: 0 436 1341 Size: 436 905 XmaI C'CCGG_G Cuts at: 0 344 1341 Size: 344 997 Enzymes that do cut: AarI AccI Acc65I AcuI AflIII AgeI AhdI AlwNI ApaI ApaLI ApoI AvaI AvrII BanI BanII BbsI BciVI BclI BfrBI Bme1580I BmrI BpmI BsaI BsaHI BsaWI BseYI BsiEI BsiHKAI BsmI Bsp1286I BspMI BsrDI BsrFI BssSI BstBI BstXI BstYI Bsu36I BtgI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I Hin4I HincII KpnI MmeI MspA1I NaeI NgoMIV NotI NsiI NspI PasI PciI PpuMI PsiI PspOMI PstI PvuII SacII SalI SfcI SmaI StyI TaqII TatI TstI Tth111I XmaI Enzymes that do not cut: AatII AclI AfeI AflII AleI AlfI AloI AscI AseI AsiSI BaeI BamHI BbeI BbvCI BcgI BglI BglII BlpI BmgBI BmtI BplI Bpu10I BpuEI BsaAI BsaBI BsaXI BseRI BsgI BsiWI BsmBI BspEI BspHI BsrBI BsrGI BssHII BstAPI BstEII BstZ17I BtgZI BtsI ClaI CspCI DraI DraIII DrdI EcoICRI EcoRI EcoRV FalI FseI FspI FspAI HaeII HindIII HpaI KasI MfeI MluI MscI MslI NarI NcoI NdeI NheI NruI PacI PflMI PfoI PmeI PmlI PpiI PshAI PspXI PsrI PvuI RsrII SacI SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmlI SnaBI SpeI SphI SrfI SspI StuI SwaI XbaI XcmI XhoI XmnI ZraI