################################## # NO CUT ENZYMES ( 165 enzymes) # ################################## Acc65I AccI AccII AclI AcsI AfaI AflII AgeI Alw44I AlwI AlwNI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp718I AspHI AspI AvaI AviII BalI BamHI BbeI BbrPI BbvCI BcgI BciVI BclI BfrI BglII BlpI BpmI Bpu1102I BsaAI BsaI BsgI BsiEI BsiHKAI BsiWI BsmFI BsmI BsoBI Bsp1407I BspDI BspLU11I BspMI BsrDI BsrFI BsrGI BsrI BssHII BssSI Bst1107I BstAPI BstBI BstF5I BstUI BstXI BstYI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DraI DraIII EaeI EagI EclXI Eco47III Eco52I Eco81I EcoNI EcoRI EcoRV EcoT22I FbaI FokI FseI FspI HaeII HhaI HinP1I HincII HindII HindIII HinfI HpaI KasI KpnI KspI MfeI MflI MluI MluNI MscI MseI MunI MvnI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI RsaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfaNI SfcI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TfiI Tru9I Tsp509I TspRI Tth111I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI ################################### # ONE CUT ENZYMES ( 40 enzymes) # ################################### AatII 402 GACGTC GACGT^C AccIII 491 TCCGGA T^CCGGA AflIII 474 ACACGT A^C[GA][CT]GT AhdI 397 GACCCGACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspEI 397 GACCCGACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AvrII 231 CCTAGG C^CTAGG BanI 346 GGCACC G^G[CT][GA]CC BanII 181 GAGCCC G[GA]GC[CT]^C BbsI 280 GAAGAC GAAGAC(2/6) BfaI 232 CTAG C^TAG BglI 97 GCCCTCCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlnI 231 CCTAGG C^CTAGG BpuAI 280 GAAGAC GAAGAC(2/6) BsaBI 501 GATCAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaWI 491 TCCGGA [AT]^CCGG[AT] BseAI 491 TCCGGA T^CCGGA BsmBI 236 GAGACG CGTCTC(1/5) BspEI 491 TCCGGA T^CCGGA BspHI 353 TCATGA T^CATGA BsrBI 29 GAGCGG CCGCTC(-3/-3) BstEII 408 GGTGACC G^GT[ACGT]ACC BstPI 408 GGTGACC G^GT[ACGT]ACC DpnII 501 GATC ^GATC DsaI 119 CCATGG C^C[GA][CT]GG Eam1105I 397 GACCCGACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoO65I 408 GGTGACC G^GT[ACGT]ACC MaeI 232 CTAG C^TAG MamI 501 GATCAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MboI 501 GATC ^GATC MroI 491 TCCGGA T^CCGGA MspA1I 379 CAGCTG C[AC]G^C[GT]G NcoI 119 CCATGG C^CATGG NdeII 501 GATC ^GATC NspI 137 GCATGT [GA]CATG^[CT] PpuMI 110 AGGACCC [GA]G^G[AT]CC[CT] PvuII 379 CAGCTG CAG^CTG RcaI 353 TCATGA T^CATGA Sau3AI 501 GATC ^GATC TaqI 314 TCGA T^CGA TthHB8I 314 TCGA T^CGA ################################### # TWO CUT ENZYMES ( 15 enzymes) # ################################### Asp700I 11 GAACCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 357 GAACACCTTC AvaII 111 GGACC G^G[AT]CC 396 GGACC BsmAI 236 GAGAC GTCTC(1/5) 266 GAGAC BstNI 108 CCAGG CC^[AT]GG 186 CCTGG DdeI 133 CTGAG C^T[ACGT]AG 326 CTGAG DraII 110 AGGACCC [GA]G^G[ACGT]CC[CT] 128 AGGCCCT DrdI 283 GACATCATTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 343 GACGGCACCGTC EcoO109I 110 AGGACCC [GA]G^G[ACGT]CC[CT] 128 AGGCCCT EcoRII 108 CCAGG ^CC[AT]GG 186 CCTGG EcoT14I 119 CCATGG C^C[AT][AT]GG 231 CCTAGG HgaI 238 GACGC GACGC(5/10) 456 GCGTC MslI 312 CATCGAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 348 CACCGTCATG MvaI 108 CCAGG CC^[AT]GG 186 CCTGG StyI 119 CCATGG C^C[AT][AT]GG 231 CCTAGG XmnI 11 GAACCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 357 GAACACCTTC ######################################### # 3 OR MORE CUT ENZYMES ( 38 enzymes) # ######################################### AciI AcyI AluI BbvI BcnI BmyI BsaHI BsaJI BseRI BsiYI BslI Bsp1286I BssKI Cac8I Cfr13I EarI FauI Fnu4HI HaeIII HapII Hin1I HpaII HphI ItaI Ksp632I MaeII MaeIII MboII MnlI MspI MwoI NciI NlaIII NlaIV Sau96I ScrFI TseI Tsp45I