(Circular) (Six-base) MAPSORT of: pFN21AB5769.seq Check: 9461 from: 1 to: 6955 pFN21AB5769 6955 bp With 182 enzymes: SgfI * April 15, 2010 09:23 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2119 2119 Size: 6955 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4765 278 Size: 53 83 186 936 3229 2468 Fragments arranged by size: 3229 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4298 1937 Size: 2361 4594 Acc65I G'GTAC_C Cuts at: 4062 4276 4062 Size: 214 6741 AclI AA'CG_TT Cuts at: 1563 5082 5455 1563 Size: 3519 373 3063 Fragments arranged by size: 3519 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2163 2483 2813 2818 3926 5018 6402 6670 Size: 531 320 330 5 1108 1092 1384 268 Cuts at: 6670 1632 Size: 1917 Fragments arranged by size: 1917 1384 1108 1092 531 330 320 268 5 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6758 AflIII A'CryG_T Cuts at: 1203 5854 6831 1203 Size: 4651 977 1327 Fragments arranged by size: 4651 1327 977 AgeI A'CCGG_T Cuts at: 5786 5786 Size: 6955 AhdI GACnn_n'nnGTC Cuts at: 2515 5684 2515 Size: 3169 3786 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6955 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3227 1184 Size: 2043 4912 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2753 2785 2753 Size: 32 6923 AlwNI CAG_nnn'CTG Cuts at: 2627 2650 2671 2791 2953 3937 4698 6270 2627 Size: 23 21 120 162 984 761 1572 3312 Fragments arranged by size: 3312 1572 984 761 162 120 23 21 ApaI G_GGCC'C Cuts at: 2057 3044 3516 2057 Size: 987 472 5496 Fragments arranged by size: 5496 987 472 ApaLI G'TGCA_C Cuts at: 5012 6168 5012 Size: 1156 5799 ApoI r'AATT_y Cuts at: 1493 2704 4264 4490 4642 6751 1493 Size: 1211 1560 226 152 2109 1697 Fragments arranged by size: 2109 1697 1560 1211 226 152 AseI AT'TA_AT Cuts at: 160 1032 4022 5509 160 Size: 872 2990 1487 1606 Fragments arranged by size: 2990 1606 1487 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6955 AvaI C'yCGr_G Cuts at: 1870 1943 2070 4280 1870 Size: 73 127 2210 4545 Fragments arranged by size: 4545 2210 127 73 AvrII C'CTAG_G Cuts at: 2044 2044 Size: 6955 BamHI G'GATC_C Cuts at: 2040 2075 4285 4652 2040 Size: 35 2210 367 4343 Fragments arranged by size: 4343 2210 367 35 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3282 3521 3667 3940 4062 Size: 325 217 627 1495 239 146 273 122 Cuts at: 4062 4276 5731 618 Size: 214 1455 1842 Fragments arranged by size: 1842 1495 1455 627 325 273 239 217 214 146 122 BanII G_rGCy'C Cuts at: 1391 2057 2380 2453 2568 3044 3410 3503 3516 Size: 666 323 73 115 476 366 93 13 Cuts at: 3516 3904 4274 1391 Size: 388 370 4072 Fragments arranged by size: 4072 666 476 388 370 366 323 115 93 73 13 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2514 928 Size: 971 615 5369 Fragments arranged by size: 5369 971 615 BbvCI CC'TCA_GC Cuts at: 2888 3485 2888 Size: 597 6358 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 2915 2949 5146 5180 1903 Size: 34 50 34 894 34 2197 34 3678 Fragments arranged by size: 3678 2197 894 50 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4847 6057 4847 Size: 1210 5745 BclI T'GATC_A Cuts at: 1548 1548 Size: 6955 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2927 5566 136 Size: 107 122 71 631 605 1255 2639 1525 Fragments arranged by size: 2639 1525 1255 631 605 122 107 71 BglII A'GATC_T Cuts at: 2837 6950 2837 Size: 4113 2842 BlpI GC'TnA_GC Cuts at: 3425 4372 3425 Size: 947 6008 Bme1580I G_kGCm'C Cuts at: 1790 2057 2092 3044 3516 3672 5016 6172 1790 Size: 267 35 952 472 156 1344 1156 2573 Fragments arranged by size: 2573 1344 1156 952 472 267 156 35 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6955 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2121 3046 4686 Size: 416 53 83 399 24 704 925 1640 Cuts at: 4686 5644 442 Size: 958 1753 Fragments arranged by size: 1753 1640 958 925 704 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2388 1056 Size: 1332 5623 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3360 3360 Size: 6955 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2366 2901 3410 4091 5615 1239 Size: 1127 535 509 681 1524 2579 Fragments arranged by size: 2579 1524 1127 681 535 509 Bpu10I CC'TnA_GC Cuts at: 2379 2888 3026 3485 2379 Size: 509 138 459 5849 Fragments arranged by size: 5849 509 459 138 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 5080 5945 6243 5080 Size: 865 298 5792 Fragments arranged by size: 5792 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3565 3606 5618 882 Size: 617 2066 41 2012 2219 Fragments arranged by size: 2219 2066 2012 617 41 BsaAI yAC'GTr Cuts at: 493 1204 4746 493 Size: 711 3542 2702 Fragments arranged by size: 3542 2702 711 BsaBI GATnn'nnATC Cuts at: 4107 6949 4107 Size: 2842 4113 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2200 4762 5144 5779 Size: 53 83 186 936 667 2562 382 635 Cuts at: 5779 275 Size: 1451 Fragments arranged by size: 2562 1451 936 667 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3896 5388 5786 6060 6207 6618 3896 Size: 1492 398 274 147 411 4233 Fragments arranged by size: 4233 1492 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2339 2369 2753 2783 3449 Size: 30 165 30 936 30 384 30 666 Cuts at: 3449 3479 3651 3681 4050 4080 1178 Size: 30 172 30 369 30 4053 Fragments arranged by size: 4053 936 666 384 369 172 165 30 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2058 2363 2391 3100 3464 1186 Size: 872 305 28 709 364 4677 Fragments arranged by size: 4677 872 709 364 305 28 BseYI C'CCAG_C Cuts at: 2050 2079 2239 2453 2547 3117 3671 3930 4183 Size: 29 160 214 94 570 554 259 253 Cuts at: 4183 6158 6654 2050 Size: 1975 496 2351 Fragments arranged by size: 2351 1975 570 554 496 259 253 214 160 94 29 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3197 1163 Size: 573 1461 4921 Fragments arranged by size: 4921 1461 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3184 3351 3355 4405 5166 6194 Size: 275 186 1183 167 4 1050 761 1028 Cuts at: 6194 1540 Size: 2301 Fragments arranged by size: 2301 1183 1050 1028 761 275 186 167 4 BsiHKAI G_wGCw'C Cuts at: 3503 5016 5101 6172 3503 Size: 1513 85 1071 4286 Fragments arranged by size: 4286 1513 1071 85 BsmI GAATG_Cn' Cuts at: 4466 4559 4466 Size: 93 6862 BsmBI CGTCTCn'nnnn_ Cuts at: 2750 2750 Size: 6955 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2057 2092 2380 2453 2568 3044 3410 Size: 399 267 35 288 73 115 476 366 Cuts at: 3410 3503 3516 3672 3904 4274 5016 5101 6172 Size: 93 13 156 232 370 742 85 1071 Cuts at: 6172 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 476 399 370 366 288 267 232 156 115 93 85 73 35 13 BspEI T'CCGG_A Cuts at: 6618 6618 Size: 6955 BspHI T'CATG_A Cuts at: 4844 6574 4844 Size: 1730 5225 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2119 3165 4310 844 Size: 1275 1046 1145 3489 Fragments arranged by size: 3489 1275 1145 1046 BsrBI CCG'CTC Cuts at: 1394 1823 3310 3598 3744 4842 1394 Size: 429 1487 288 146 1098 3507 Fragments arranged by size: 3507 1487 1098 429 288 146 BsrDI GCAATG_nn' Cuts at: 65 1248 3690 5450 5624 65 Size: 1183 2442 1760 174 1396 Fragments arranged by size: 2442 1760 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2016 3135 3350 5599 5786 1700 Size: 110 206 1119 215 2249 187 2869 Fragments arranged by size: 2869 2249 1119 215 206 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6955 BssHII G'CGCG_C Cuts at: 1925 3288 1925 Size: 1363 5592 BssSI C'ACGA_G Cuts at: 3236 3260 5015 6027 3236 Size: 24 1755 1012 4164 Fragments arranged by size: 4164 1755 1012 24 BstAPI GCAn_nnn'nTGC Cuts at: 794 4193 794 Size: 3399 3556 BstBI TT'CG_AA Cuts at: 2167 5759 2167 Size: 3592 3363 BstEII G'GTnAC_C Cuts at: 1184 3799 3892 1184 Size: 2615 93 4247 Fragments arranged by size: 4247 2615 93 BstXI CCAn_nnnn'nTGG Cuts at: 2034 3368 2034 Size: 1334 5621 BstYI r'GATC_y Cuts at: 1972 2040 2075 2492 2837 4285 4652 5037 5054 Size: 68 35 417 345 1448 367 385 17 Cuts at: 5054 6506 6592 6604 6950 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1448 417 385 367 346 345 86 68 35 17 12 Bsu36I CC'TnA_GG Cuts at: 2820 3112 2820 Size: 292 6663 BtgI C'CryG_G Cuts at: 513 1065 2772 3417 513 Size: 552 1707 645 4051 Fragments arranged by size: 4051 1707 645 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3547 487 Size: 683 56 2321 3895 Fragments arranged by size: 3895 2321 683 56 BtsI GCAGTG_nn' Cuts at: 3762 3947 4004 4471 5265 5285 3762 Size: 185 57 467 794 20 5432 Fragments arranged by size: 5432 794 467 185 57 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6955 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6920 DraI TTT'AAA Cuts at: 4259 4259 Size: 6955 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3846 5962 809 Size: 507 2530 2116 1802 Fragments arranged by size: 2530 2116 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2024 2122 2299 Size: 54 1425 50 275 18 194 98 177 Cuts at: 2299 3181 3232 3352 4160 4402 5291 6920 8 Size: 882 51 120 808 242 889 1629 43 Fragments arranged by size: 1629 1425 889 882 808 275 242 194 177 120 98 54 51 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3181 3352 4402 1537 Size: 275 1369 171 1050 4090 Fragments arranged by size: 4090 1369 1050 275 171 EarI CTCTTCn'nnn_ Cuts at: 1353 2789 3441 3563 4253 4885 1353 Size: 1436 652 122 690 632 3423 Fragments arranged by size: 3423 1436 690 652 632 122 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5542 5916 6062 387 Size: 1064 138 3953 374 146 1280 Fragments arranged by size: 3953 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3501 3501 Size: 6955 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2163 2366 2483 2813 2818 2901 3410 Size: 393 531 203 117 330 5 83 509 Cuts at: 3410 3926 4091 5018 5615 6402 6670 1239 Size: 516 165 927 597 787 268 1524 Fragments arranged by size: 1524 927 787 597 531 516 509 393 330 268 203 165 117 83 5 EcoNI CCTnn'n_nnAGG Cuts at: 2501 2501 Size: 6955 EcoO109I rG'GnC_Cy Cuts at: 2054 2362 2543 2624 2850 2929 3040 3512 2054 Size: 308 181 81 226 79 111 472 5497 Fragments arranged by size: 5497 472 308 226 181 111 81 79 EcoRI G'AATT_C Cuts at: 4264 4642 4264 Size: 378 6577 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3790 3790 Size: 6955 FspI TGC'GCA Cuts at: 3542 5461 3542 Size: 1919 5036 HaeII r_GCGC'y Cuts at: 1678 3107 3432 6102 1678 Size: 1429 325 2670 2531 Fragments arranged by size: 2670 2531 1429 325 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2067 2099 2339 2371 2501 Size: 32 135 32 495 32 240 32 130 Cuts at: 2501 2533 2666 2698 3358 3390 3449 3481 5596 Size: 32 133 32 660 32 59 32 2115 Cuts at: 5596 5628 5670 5702 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2115 660 495 240 135 133 130 59 42 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3060 4299 4545 669 Size: 988 281 1122 1239 246 3079 Fragments arranged by size: 3079 1239 1122 988 281 246 HindIII A'AGCT_T Cuts at: 748 3767 3908 748 Size: 3019 141 3795 Fragments arranged by size: 3795 3019 141 HpaI GTT'AAC Cuts at: 3060 4545 3060 Size: 1485 5470 KpnI G_GTAC'C Cuts at: 4066 4280 4066 Size: 214 6741 MfeI C'AATT_G Cuts at: 2303 4554 2303 Size: 2251 4704 MluI A'CGCG_T Cuts at: 6831 6831 Size: 6955 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2205 2306 3234 3371 3435 3615 6069 6253 Size: 900 101 928 137 64 180 2454 184 Cuts at: 6253 1305 Size: 2007 Fragments arranged by size: 2454 2007 928 900 184 180 137 101 64 MscI TGG'CCA Cuts at: 10 64 1489 1832 2026 2124 3234 10 Size: 54 1425 343 194 98 1110 3731 Fragments arranged by size: 3731 1425 1110 343 194 98 54 MslI CAynn'nnrTG Cuts at: 518 1166 3887 3966 4913 5272 5431 518 Size: 648 2721 79 947 359 159 2042 Fragments arranged by size: 2721 2042 947 648 359 159 79 MspA1I CmG'CkG Cuts at: 1606 2601 3128 3419 3475 3652 4213 4222 4371 Size: 995 527 291 56 177 561 9 149 Cuts at: 4371 5048 6196 6441 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1148 995 677 561 527 291 245 177 149 56 9 NaeI GCC'GGC Cuts at: 3137 3137 Size: 6955 NcoI C'CATG_G Cuts at: 513 1065 2772 513 Size: 552 1707 4696 Fragments arranged by size: 4696 1707 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6955 NgoMIV G'CCGG_C Cuts at: 3135 3135 Size: 6955 NheI G'CTAG_C Cuts at: 1052 2384 1052 Size: 1332 5623 NotI GC'GGCC_GC Cuts at: 4402 4402 Size: 6955 NruI TCG'CGA Cuts at: 3566 3566 Size: 6955 NspI r_CATG'y Cuts at: 1132 1229 2634 4313 5858 1132 Size: 97 1405 1679 1545 2229 Fragments arranged by size: 2229 1679 1545 1405 97 PasI CC'CwG_GG Cuts at: 1348 4205 1348 Size: 2857 4098 PciI A'CATG_T Cuts at: 5854 5854 Size: 6955 PflMI CCAn_nnn'nTGG Cuts at: 1108 2019 2971 3937 4129 1108 Size: 911 952 966 192 3934 Fragments arranged by size: 3934 966 952 911 192 PfoI T'CCnGG_A Cuts at: 2472 2726 2952 3918 4068 2472 Size: 254 226 966 150 5359 Fragments arranged by size: 5359 966 254 226 150 PmeI GTTT'AAAC Cuts at: 4259 4259 Size: 6955 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2753 2785 5024 5056 6537 6569 2753 Size: 32 2239 32 1481 32 3139 Fragments arranged by size: 3139 2239 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2850 2850 Size: 6955 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6955 PsiI TTA'TAA Cuts at: 4525 4525 Size: 6955 PspOMI G'GGCC_C Cuts at: 2053 3040 3512 2053 Size: 987 472 5496 Fragments arranged by size: 5496 987 472 PstI C_TGCA'G Cuts at: 830 2646 3252 3594 4307 830 Size: 1816 606 342 713 3478 Fragments arranged by size: 3478 1816 713 606 342 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6955 PvuII CAG'CTG Cuts at: 4213 4222 4213 Size: 9 6946 SacI G_AGCT'C Cuts at: 3503 3503 Size: 6955 SacII CC_GC'GG Cuts at: 3420 3420 Size: 6955 SalI G'TCGA_C Cuts at: 1936 4297 1936 Size: 2361 4594 SanDI GG'GwC_CC Cuts at: 2850 2850 Size: 6955 SbfI CC_TGCA'GG Cuts at: 3594 4307 3594 Size: 713 6242 ScaI AGT'ACT Cuts at: 3868 5203 6915 3868 Size: 1335 1712 3908 Fragments arranged by size: 3908 1712 1335 SexAI A'CCwGG_T Cuts at: 2583 3615 2583 Size: 1032 5923 SfcI C'TryA_G Cuts at: 826 1045 2642 3248 3590 4303 5438 6119 6310 Size: 219 1597 606 342 713 1135 681 191 Cuts at: 6310 826 Size: 1471 Fragments arranged by size: 1597 1471 1135 713 681 606 342 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6955 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6955 SmaI CCC'GGG Cuts at: 1872 4282 1872 Size: 2410 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 5059 5960 6222 820 Size: 197 926 3116 901 262 1553 Fragments arranged by size: 3116 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4746 493 Size: 4253 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6955 SphI G_CATG'C Cuts at: 1132 2634 4313 1132 Size: 1502 1679 3774 Fragments arranged by size: 3774 1679 1502 SspI AAT'ATT Cuts at: 5 52 4879 5 Size: 47 4827 2081 Fragments arranged by size: 4827 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6955 StyI C'CwwG_G Cuts at: 513 1065 2044 2772 3010 3361 4394 513 Size: 552 979 728 238 351 1033 3074 Fragments arranged by size: 3074 1033 979 728 552 351 238 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2598 3040 3313 4982 4999 5152 5337 Size: 1255 1018 442 273 1669 17 153 185 Cuts at: 5337 5782 325 Size: 445 1498 Fragments arranged by size: 1669 1498 1255 1018 445 442 273 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2574 3866 5201 Size: 274 80 33 51 1443 597 1292 1335 Cuts at: 5201 6913 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1335 1292 597 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3651 3683 6915 6947 3651 Size: 32 3232 32 3659 Fragments arranged by size: 3659 3232 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6955 XbaI T'CTAG_A Cuts at: 3715 4291 3715 Size: 576 6379 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6955 XmaI C'CCGG_G Cuts at: 1870 4280 1870 Size: 2410 4545 XmnI GAAnn'nnTTC Cuts at: 2468 5084 2468 Size: 2616 4339 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4763 276 Size: 53 83 186 936 3229 2468 Fragments arranged by size: 3229 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NruI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SanDI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI BaeI BbeI BfrBI BsiWI BstZ17I DraIII EcoRV FseI FspAI KasI NarI NsiI PacI PmlI PspXI PsrI RsrII SapI SfiI SfoI SrfI SwaI XcmI