Restriction map for
pFN21AB5769
(Linear) (Six-base) MAPSORT of: KIBB5769.seq Check: 5093 from: 1 to: 2250
KIBB5769.orf
With 182 enzymes: SgfI *
April 24, 2009 13:05 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 114 2250
Size: 114 2136
Acc65I G'GTAC_C
Cuts at: 0 2057 2250
Size: 2057 193
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 158 478 808 813 1921 2250
Size: 158 320 330 5 1108 329
Fragments arranged by size:
1108 330 329 320 158 5
AhdI GACnn_n'nnGTC
Cuts at: 0 510 2250
Size: 510 1740
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1222 2250
Size: 1222 1028
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 748 780 2250
Size: 748 32 1470
Fragments arranged by size:
1470 748 32
AlwNI CAG_nnn'CTG
Cuts at: 0 622 645 666 786 948 1932 2250
Size: 622 23 21 120 162 984 318
Fragments arranged by size:
984 622 318 162 120 23 21
ApaI G_GGCC'C
Cuts at: 0 52 1039 1511 2250
Size: 52 987 472 739
Fragments arranged by size:
987 739 472 52
ApoI r'AATT_y
Cuts at: 0 699 2250
Size: 699 1551
AseI AT'TA_AT
Cuts at: 0 2017 2250
Size: 2017 233
AvaI C'yCGr_G
Cuts at: 0 65 2250
Size: 65 2185
AvrII C'CTAG_G
Cuts at: 0 39 2250
Size: 39 2211
BamHI G'GATC_C
Cuts at: 0 35 70 2250
Size: 35 35 2180
Fragments arranged by size:
2180 35 35
BanI G'GyrC_C
Cuts at: 0 1277 1516 1662 1935 2057 2250
Size: 1277 239 146 273 122 193
Fragments arranged by size:
1277 273 239 193 146 122
BanII G_rGCy'C
Cuts at: 0 52 375 448 563 1039 1405 1498 1511
Size: 52 323 73 115 476 366 93 13
Cuts at: 1511 1899 2250
Size: 388 351
Fragments arranged by size:
476 388 366 351 323 115 93 73
52 13
BbsI GAAGACnn'nnnn_
Cuts at: 0 509 2250
Size: 509 1741
BbvCI CC'TCA_GC
Cuts at: 0 883 1480 2250
Size: 883 597 770
Fragments arranged by size:
883 770 597
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 910 944 2250
Size: 910 34 1306
Fragments arranged by size:
1306 910 34
BglI GCCn_nnn'nGGC
Cuts at: 0 922 2250
Size: 922 1328
BglII A'GATC_T
Cuts at: 0 832 2250
Size: 832 1418
BlpI GC'TnA_GC
Cuts at: 0 1420 2250
Size: 1420 830
Bme1580I G_kGCm'C
Cuts at: 0 52 87 1039 1511 1667 2250
Size: 52 35 952 472 156 583
Fragments arranged by size:
952 583 472 156 52 35
BmrI ACTGGGnnnn_n'
Cuts at: 0 116 1041 2250
Size: 116 925 1209
Fragments arranged by size:
1209 925 116
BmtI G_CTAG'C
Cuts at: 0 383 2250
Size: 383 1867
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1355 2250
Size: 1355 895
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 361 896 1405 2086 2250
Size: 361 535 509 681 164
Fragments arranged by size:
681 535 509 361 164
Bpu10I CC'TnA_GC
Cuts at: 0 374 883 1021 1480 2250
Size: 374 509 138 459 770
Fragments arranged by size:
770 509 459 374 138
BsaI GGTCTCn'nnnn_
Cuts at: 0 1560 1601 2250
Size: 1560 41 649
Fragments arranged by size:
1560 649 41
BsaBI GATnn'nnATC
Cuts at: 0 2102 2250
Size: 2102 148
BsaHI Gr'CG_yC
Cuts at: 0 195 2250
Size: 195 2055
BsaWI w'CCGG_w
Cuts at: 0 1891 2250
Size: 1891 359
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 334 364 748 778 1444 1474 1646 1676
Size: 334 30 384 30 666 30 172 30
Cuts at: 1676 2045 2075 2250
Size: 369 30 175
Fragments arranged by size:
666 384 369 334 175 172 30 30
30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 53 358 386 1095 1459 2250
Size: 53 305 28 709 364 791
Fragments arranged by size:
791 709 364 305 53 28
BseYI C'CCAG_C
Cuts at: 0 45 74 234 448 542 1112 1666 1925
Size: 45 29 160 214 94 570 554 259
Cuts at: 1925 2178 2250
Size: 253 72
Fragments arranged by size:
570 554 259 253 214 160 94 72
45 29
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1192 2250
Size: 1192 1058
BsiEI CG_ry'CG
Cuts at: 0 1179 1346 1350 2250
Size: 1179 167 4 900
Fragments arranged by size:
1179 900 167 4
BsiHKAI G_wGCw'C
Cuts at: 0 1498 2250
Size: 1498 752
BsmBI CGTCTCn'nnnn_
Cuts at: 0 745 2250
Size: 745 1505
Bsp1286I G_dGCh'C
Cuts at: 0 52 87 375 448 563 1039 1405 1498
Size: 52 35 288 73 115 476 366 93
Cuts at: 1498 1511 1667 1899 2250
Size: 13 156 232 351
Fragments arranged by size:
476 366 351 288 232 156 115 93
73 52 35 13
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 114 1160 2250
Size: 114 1046 1090
Fragments arranged by size:
1090 1046 114
BsrBI CCG'CTC
Cuts at: 0 1305 1593 1739 2250
Size: 1305 288 146 511
Fragments arranged by size:
1305 511 288 146
BsrDI GCAATG_nn'
Cuts at: 0 1685 2250
Size: 1685 565
BsrFI r'CCGG_y
Cuts at: 0 11 1130 1345 2250
Size: 11 1119 215 905
Fragments arranged by size:
1119 905 215 11
BssHII G'CGCG_C
Cuts at: 0 1283 2250
Size: 1283 967
BssSI C'ACGA_G
Cuts at: 0 1231 1255 2250
Size: 1231 24 995
Fragments arranged by size:
1231 995 24
BstAPI GCAn_nnn'nTGC
Cuts at: 0 2188 2250
Size: 2188 62
BstBI TT'CG_AA
Cuts at: 0 162 2250
Size: 162 2088
BstEII G'GTnAC_C
Cuts at: 0 1794 1887 2250
Size: 1794 93 363
Fragments arranged by size:
1794 363 93
BstXI CCAn_nnnn'nTGG
Cuts at: 0 29 1363 2250
Size: 29 1334 887
Fragments arranged by size:
1334 887 29
BstYI r'GATC_y
Cuts at: 0 35 70 487 832 2250
Size: 35 35 417 345 1418
Fragments arranged by size:
1418 417 345 35 35
Bsu36I CC'TnA_GG
Cuts at: 0 815 1107 2250
Size: 815 292 1143
Fragments arranged by size:
1143 815 292
BtgI C'CryG_G
Cuts at: 0 767 1412 2250
Size: 767 645 838
Fragments arranged by size:
838 767 645
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1542 2250
Size: 1542 708
BtsI GCAGTG_nn'
Cuts at: 0 1757 1942 1999 2250
Size: 1757 185 57 251
Fragments arranged by size:
1757 251 185 57
DrdI GACnn_nn'nnGTC
Cuts at: 0 1841 2250
Size: 1841 409
EaeI y'GGCC_r
Cuts at: 0 19 117 294 1176 1227 1347 2155 2250
Size: 19 98 177 882 51 120 808 95
Fragments arranged by size:
882 808 177 120 98 95 51 19
EagI C'GGCC_G
Cuts at: 0 1176 1347 2250
Size: 1176 171 903
Fragments arranged by size:
1176 903 171
EarI CTCTTCn'nnn_
Cuts at: 0 784 1436 1558 2248 2250
Size: 784 652 122 690 2
Fragments arranged by size:
784 690 652 122 2
EcoICRI GAG'CTC
Cuts at: 0 1496 2250
Size: 1496 754
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 158 361 478 808 813 896 1405 1921
Size: 158 203 117 330 5 83 509 516
Cuts at: 1921 2086 2250
Size: 165 164
Fragments arranged by size:
516 509 330 203 165 164 158 117
83 5
EcoNI CCTnn'n_nnAGG
Cuts at: 0 496 2250
Size: 496 1754
EcoO109I rG'GnC_Cy
Cuts at: 0 49 357 538 619 845 924 1035 1507
Size: 49 308 181 81 226 79 111 472
Cuts at: 1507 2250
Size: 743
Fragments arranged by size:
743 472 308 226 181 111 81 79
49
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1785 2250
Size: 1785 465
FspI TGC'GCA
Cuts at: 0 1537 2250
Size: 1537 713
HaeII r_GCGC'y
Cuts at: 0 1102 1427 2250
Size: 1102 325 823
Fragments arranged by size:
1102 823 325
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 62 94 334 366 496 528 661 693
Size: 62 32 240 32 130 32 133 32
Cuts at: 693 1353 1385 1444 1476 2250
Size: 660 32 59 32 774
Fragments arranged by size:
774 660 240 133 130 62 59 32
32 32 32 32 32
HincII GTy'rAC
Cuts at: 0 1055 2250
Size: 1055 1195
HindIII A'AGCT_T
Cuts at: 0 1762 1903 2250
Size: 1762 141 347
Fragments arranged by size:
1762 347 141
HpaI GTT'AAC
Cuts at: 0 1055 2250
Size: 1055 1195
KpnI G_GTAC'C
Cuts at: 0 2061 2250
Size: 2061 189
MfeI C'AATT_G
Cuts at: 0 298 2250
Size: 298 1952
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 200 301 1229 1366 1430 1610 2250
Size: 200 101 928 137 64 180 640
Fragments arranged by size:
928 640 200 180 137 101 64
MscI TGG'CCA
Cuts at: 0 21 119 1229 2250
Size: 21 98 1110 1021
Fragments arranged by size:
1110 1021 98 21
MslI CAynn'nnrTG
Cuts at: 0 1882 1961 2250
Size: 1882 79 289
Fragments arranged by size:
1882 289 79
MspA1I CmG'CkG
Cuts at: 0 596 1123 1414 1470 1647 2208 2217 2250
Size: 596 527 291 56 177 561 9 33
Fragments arranged by size:
596 561 527 291 177 56 33 9
NaeI GCC'GGC
Cuts at: 0 1132 2250
Size: 1132 1118
NcoI C'CATG_G
Cuts at: 0 767 2250
Size: 767 1483
NgoMIV G'CCGG_C
Cuts at: 0 1130 2250
Size: 1130 1120
NheI G'CTAG_C
Cuts at: 0 379 2250
Size: 379 1871
NruI TCG'CGA
Cuts at: 0 1561 2250
Size: 1561 689
NspI r_CATG'y
Cuts at: 0 629 2250
Size: 629 1621
PasI CC'CwG_GG
Cuts at: 0 2200 2250
Size: 2200 50
PflMI CCAn_nnn'nTGG
Cuts at: 0 14 966 1932 2124 2250
Size: 14 952 966 192 126
Fragments arranged by size:
966 952 192 126 14
PfoI T'CCnGG_A
Cuts at: 0 467 721 947 1913 2063 2250
Size: 467 254 226 966 150 187
Fragments arranged by size:
966 467 254 226 187 150
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 748 780 2250
Size: 748 32 1470
Fragments arranged by size:
1470 748 32
PpuMI rG'GwC_Cy
Cuts at: 0 845 2250
Size: 845 1405
PspOMI G'GGCC_C
Cuts at: 0 48 1035 1507 2250
Size: 48 987 472 743
Fragments arranged by size:
987 743 472 48
PstI C_TGCA'G
Cuts at: 0 641 1247 1589 2250
Size: 641 606 342 661
Fragments arranged by size:
661 641 606 342
PvuII CAG'CTG
Cuts at: 0 2208 2217 2250
Size: 2208 9 33
Fragments arranged by size:
2208 33 9
SacI G_AGCT'C
Cuts at: 0 1498 2250
Size: 1498 752
SacII CC_GC'GG
Cuts at: 0 1415 2250
Size: 1415 835
SanDI GG'GwC_CC
Cuts at: 0 845 2250
Size: 845 1405
SbfI CC_TGCA'GG
Cuts at: 0 1589 2250
Size: 1589 661
ScaI AGT'ACT
Cuts at: 0 1863 2250
Size: 1863 387
SexAI A'CCwGG_T
Cuts at: 0 578 1610 2250
Size: 578 1032 640
Fragments arranged by size:
1032 640 578
SfcI C'TryA_G
Cuts at: 0 637 1243 1585 2250
Size: 637 606 342 665
Fragments arranged by size:
665 637 606 342
SphI G_CATG'C
Cuts at: 0 629 2250
Size: 629 1621
StyI C'CwwG_G
Cuts at: 0 39 767 1005 1356 2250
Size: 39 728 238 351 894
Fragments arranged by size:
894 728 351 238 39
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 593 1035 1308 2250
Size: 593 442 273 942
Fragments arranged by size:
942 593 442 273
TatI w'GTAC_w
Cuts at: 0 569 1861 2250
Size: 569 1292 389
Fragments arranged by size:
1292 569 389
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1646 1678 2250
Size: 1646 32 572
Fragments arranged by size:
1646 572 32
XbaI T'CTAG_A
Cuts at: 0 1710 2250
Size: 1710 540
XmnI GAAnn'nnTTC
Cuts at: 0 463 2250
Size: 463 1787
Enzymes that do cut:
AarI Acc65I AcuI AhdI AlfI AloI AlwNI ApaI
ApoI AseI AvaI AvrII BamHI BanI BanII BbsI
BbvCI BcgI BglI BglII BlpI Bme1580I BmrI BmtI
BplI BpmI Bpu10I BsaI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspMI
BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI DrdI EaeI
EagI EarI EcoICRI Eco57MI EcoNI EcoO109I FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MmeI
MscI MslI MspA1I NaeI NcoI NgoMIV NheI NruI
NspI PasI PflMI PfoI PpiI PpuMI PspOMI PstI
PvuII SacI SacII SanDI SbfI ScaI SexAI SfcI
SphI StyI TaqII TatI TstI XbaI XmnI
Enzymes that do not cut:
AatII AccI AclI AfeI AflII AflIII AgeI AleI
ApaLI AscI AsiSI BaeI BbeI BciVI BclI BfrBI
BmgBI BpuEI BsaAI BsiWI BsmI BspEI BspHI BsrGI
BstZ17I ClaI CspCI DraI DraIII EciI EcoRI EcoRV
FseI FspAI KasI MluI NarI NdeI NotI NsiI
PacI PciI PmeI PmlI PshAI PsiI PspXI PsrI
PvuI RsrII SalI SapI SfiI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SrfI SspI StuI SwaI
Tth111I XcmI XhoI XmaI ZraI