Restriction map for
pFN21AB9974
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# NO CUT ENZYMES ( 101 enzymes) #
##################################
Acc65I AccI AclI AcsI AflII
AflIII AhdI Alw44I Aor51HI ApaLI
ApoI AscI AseI AsnI Asp718I
AspEI AviII AvrII BalI BbrPI
BclI BfaI BfrI BglII BlnI
BlpI Bpu1102I BsaBI BsiWI BspDI
BspHI BspLU11I BspMI BssHII Bst1107I
BstAPI BstBI BstZ17I CelII ClaI
CpoI DraI DraIII DrdI Eam1105I
Eco47III EcoRI EcoRV EcoT22I FbaI
FseI FspI HincII HindII HpaI
KpnI MaeI MamI MfeI MluI
MluNI MscI MseI MunI NcoI
NdeI NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacI SalI
SbfI SfiI SfuI SgrAI SnaBI
SpeI SphI Sse8387I SspI StuI
SwaI Tru9I Tsp509I XbaI XcmI
XhoI
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# ONE CUT ENZYMES ( 32 enzymes) #
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AatII 1987 GACGTC GACGT^C
AccIII 1850 TCCGGA T^CCGGA
AlwNI 13 CAGCCCCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 2113 CCTGAGG CC^T[ACGT]AGG
BbvCI 1466 CCTCAGC CCTCAGC(-5/-2)
BglI 1822 GCCGACGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 2086 TACGTG [CT]AC^GT[GA]
BseAI 1850 TCCGGA T^CCGGA
BseRI 1714 GAGGAG GAGGAG(10/8)
BsmI 590 GCATTC GAATGC(1/-1)
BspEI 1850 TCCGGA T^CCGGA
BsrDI 842 GCAATG GCAATG(2/0)
BssSI 1189 CTCGTG CACGAG(-5/-1)
BstEII 1836 GGTGACC G^GT[ACGT]ACC
BstPI 1836 GGTGACC G^GT[ACGT]ACC
BstXI 1940 CCAGCGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 2113 CCTGAGG CC^T[ACGT]AGG
EagI 1645 CGGCCG C^GGCCG
EclXI 1645 CGGCCG C^GGCCG
Eco52I 1645 CGGCCG C^GGCCG
Eco81I 2113 CCTGAGG CC^T[ACGT]AGG
EcoO65I 1836 GGTGACC G^GT[ACGT]ACC
HindIII 1732 AAGCTT A^AGCTT
MroI 1850 TCCGGA T^CCGGA
NaeI 1643 GCCGGC GCC^GGC
NgoMIV 1643 GCCGGC G^CCGGC
PflMI 1212 CCACGCGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 2104 CTGCAG CTGCA^G
SexAI 2066 ACCAGGT A^CC[AT]GGT
SfcI 2104 CTGCAG C^T[GA][CT]AG
SmlI 340 CTCAAG C^T[CT][GA]AG
Van91I 1212 CCACGCGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AgeI 247 ACCGGT A^CCGGT
797 ACCGGT
Asp700I 1554 GAACATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1731 GAAGCTTTTC
AspI 493 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
1702 GACTCGGTC
BamHI 371 GGATCC G^GATCC
1926 GGATCC
BcgI 414 CGACGCCTTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
638 GCAAGGGCGTCG
BciVI 1174 GGATAC GTATCC(6/5)
534 GTATCC
BsaI 98 GAGACC GGTCTC(1/5)
244 GAGACC
BsmBI 527 GAGACG CGTCTC(1/5)
599 GAGACG
Bsp1407I 476 TGTACA T^GTACA
1766 TGTACA
BsrGI 476 TGTACA T^GTACA
1766 TGTACA
BstYI 371 GGATCC [GA]^GATC[CT]
1926 GGATCC
EarI 88 CTCTTC CTCTTC(1/4)
973 CTCTTC
EcoNI 633 CCTGGGCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1059 CCTGGGTAAGG
EcoT14I 882 CCAAGG C^C[AT][AT]GG
1170 CCAAGG
Ksp632I 88 CTCTTC CTCTTC(1/4)
973 CTCTTC
KspI 397 CCGCGG CCGC^GG
1095 CCGCGG
MflI 371 GGATCC [GA]^GATC[CT]
1926 GGATCC
PflFI 493 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
1702 GACTCGGTC
PinAI 247 ACCGGT A^CCGGT
797 ACCGGT
SacII 397 CCGCGG CCGC^GG
1095 CCGCGG
SapI 87 GCTCTTC GCTCTTC(1/4)
972 GCTCTTC
ScaI 1070 AGTACT AGT^ACT
1613 AGTACT
SmaI 833 CCCGGG CCC^GGG
1963 CCCGGG
SspBI 476 TGTACA T^GTACA
1766 TGTACA
StyI 882 CCAAGG C^C[AT][AT]GG
1170 CCAAGG
TfiI 317 GATTC G^A[AT]TC
1622 GATTC
Tth111I 493 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
1702 GACTCGGTC
XhoII 371 GGATCC [GA]^GATC[CT]
1926 GGATCC
XmaCI 833 CCCGGG C^CCGGG
1963 CCCGGG
XmaI 833 CCCGGG C^CCGGG
1963 CCCGGG
XmnI 1554 GAACATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1731 GAAGCTTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 94 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI ApaI AspHI AvaI AvaII
BanI BanII BbeI BbsI BbvI
BcnI BmyI BpmI BpuAI BsaHI
BsaJI BsaWI BsgI BsiEI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BsrBI BsrFI BsrI BssKI
BstF5I BstNI BstUI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI EcoO109I EcoRII FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI KasI MaeII
MaeIII MboI MboII MnlI MslI
MspA1I MspI MvaI MvnI MwoI
NarI NciI NdeII NlaIII NlaIV
PleI PpuMI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfoI TaqI
TseI Tsp45I TspRI TthHB8I