(Circular) (Six-base) MAPSORT of: pFN21AB9974.seq Check: 2745 from: 1 to: 6826 pFN21AB9974 6826 bp With 182 enzymes: SgfI * April 15, 2010 09:29 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3996 4636 278 Size: 53 83 186 936 2460 640 2468 Fragments arranged by size: 2468 2460 936 640 186 83 53 AccI GT'mk_AC Cuts at: 1937 4169 1937 Size: 2232 4594 Acc65I G'GTAC_C Cuts at: 4147 4147 Size: 6826 AclI AA'CG_TT Cuts at: 1563 4953 5326 1563 Size: 3390 373 3063 Fragments arranged by size: 3390 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2297 4889 6273 6541 1632 Size: 665 2592 1384 268 1917 Fragments arranged by size: 2592 1917 1384 665 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6629 AflIII A'CryG_T Cuts at: 1203 5725 6702 1203 Size: 4522 977 1327 Fragments arranged by size: 4522 1327 977 AgeI A'CCGG_T Cuts at: 2252 2802 5657 2252 Size: 550 2855 3421 Fragments arranged by size: 3421 2855 550 AhdI GACnn_n'nnGTC Cuts at: 5555 5555 Size: 6826 AleI CACnn'nnGTG Cuts at: 1166 2087 2171 2477 3521 1166 Size: 921 84 306 1044 4471 Fragments arranged by size: 4471 1044 921 306 84 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6826 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3947 3979 3947 Size: 32 6794 AlwNI CAG_nnn'CTG Cuts at: 2023 4569 6141 2023 Size: 2546 1572 2708 Fragments arranged by size: 2708 2546 1572 ApaI G_GGCC'C Cuts at: 3291 3662 3797 3853 3291 Size: 371 135 56 6264 Fragments arranged by size: 6264 371 135 56 ApaLI G'TGCA_C Cuts at: 4883 6039 4883 Size: 1156 5670 ApoI r'AATT_y Cuts at: 1493 4135 4361 4513 6622 1493 Size: 2642 226 152 2109 1697 Fragments arranged by size: 2642 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5380 160 Size: 872 4348 1606 Fragments arranged by size: 4348 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6826 AvaI C'yCGr_G Cuts at: 1870 1943 2169 2246 2838 2997 3796 3968 4151 Size: 73 226 77 592 159 799 172 183 Cuts at: 4151 1870 Size: 4545 Fragments arranged by size: 4545 799 592 226 183 172 159 77 73 BamHI G'GATC_C Cuts at: 2376 3931 4156 4523 2376 Size: 1555 225 367 4679 Fragments arranged by size: 4679 1555 367 225 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2352 3111 3324 3756 3894 Size: 325 217 627 565 759 213 432 138 Cuts at: 3894 3973 4147 5602 618 Size: 79 174 1455 1842 Fragments arranged by size: 1842 1455 759 627 565 432 325 217 213 174 138 79 BanII G_rGCy'C Cuts at: 1391 2565 3291 3662 3797 3853 4145 1391 Size: 1174 726 371 135 56 292 4072 Fragments arranged by size: 4072 1174 726 371 292 135 56 BbeI G_GCGC'C Cuts at: 3115 3760 3898 3115 Size: 645 138 6043 Fragments arranged by size: 6043 645 138 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2400 3040 3988 928 Size: 971 501 640 948 3766 Fragments arranged by size: 3766 971 948 640 501 BbvCI CC'TCA_GC Cuts at: 3472 3472 Size: 6826 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2408 2442 2632 2666 5017 5051 1903 Size: 34 471 34 190 34 2351 34 3678 Fragments arranged by size: 3678 2351 471 190 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2550 3173 4718 5928 2550 Size: 623 1545 1210 3448 Fragments arranged by size: 3448 1545 1210 623 BclI T'GATC_A Cuts at: 1548 1548 Size: 6826 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3833 5437 136 Size: 107 122 71 631 605 2161 1604 1525 Fragments arranged by size: 2161 1604 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6821 6821 Size: 6826 BlpI GC'TnA_GC Cuts at: 4243 4243 Size: 6826 Bme1580I G_kGCm'C Cuts at: 1790 3291 3662 3797 3853 4118 4887 6043 1790 Size: 1501 371 135 56 265 769 1156 2573 Fragments arranged by size: 2573 1501 1156 769 371 265 135 56 BmgBI CAC'GTC Cuts at: 1318 3310 3832 3913 1318 Size: 1992 522 81 4231 Fragments arranged by size: 4231 1992 522 81 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4557 5515 442 Size: 416 53 83 399 24 3140 958 1753 Fragments arranged by size: 3140 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6826 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2737 3745 2737 Size: 1008 5818 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2121 2210 3922 5486 1239 Size: 882 89 1712 1564 2579 Fragments arranged by size: 2579 1712 1564 882 89 Bpu10I CC'TnA_GC Cuts at: 3351 3472 3351 Size: 121 6705 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2330 4951 5816 6114 2330 Size: 2621 865 298 3042 Fragments arranged by size: 3042 2621 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2097 2243 5489 882 Size: 617 598 146 3246 2219 Fragments arranged by size: 3246 2219 617 598 146 BsaAI yAC'GTr Cuts at: 493 1204 4093 4617 493 Size: 711 2889 524 2702 Fragments arranged by size: 2889 2702 711 524 BsaBI GATnn'nnATC Cuts at: 6820 6820 Size: 6826 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2421 2459 2607 2649 Size: 53 83 186 936 888 38 148 42 Cuts at: 2649 3112 3570 3757 3802 3895 3993 4633 5015 Size: 463 458 187 45 93 98 640 382 Cuts at: 5015 5650 275 Size: 635 1451 Fragments arranged by size: 1451 936 888 640 635 463 458 382 187 186 148 98 93 83 53 45 42 38 BsaWI w'CCGG_w Cuts at: 2252 2802 3413 3535 3855 3957 5259 5657 5931 Size: 550 611 122 320 102 1302 398 274 Cuts at: 5931 6078 6489 2252 Size: 147 411 2589 Fragments arranged by size: 2589 1302 611 550 411 398 320 274 147 122 102 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2735 2765 2909 2939 3816 Size: 30 165 30 1332 30 144 30 877 Cuts at: 3816 3846 1178 Size: 30 4158 Fragments arranged by size: 4158 1332 877 165 144 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3734 1186 Size: 2548 4278 BseYI C'CCAG_C Cuts at: 2044 2819 3223 3790 3814 6029 6525 2044 Size: 775 404 567 24 2215 496 2345 Fragments arranged by size: 2345 2215 775 567 496 404 24 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2719 2791 2893 1163 Size: 573 983 72 102 5096 Fragments arranged by size: 5096 983 573 102 72 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2749 2803 2923 2968 3653 4276 Size: 275 186 748 54 120 45 685 623 Cuts at: 4276 5037 6065 1540 Size: 761 1028 2301 Fragments arranged by size: 2301 1028 761 748 685 623 275 186 120 54 45 BsiHKAI G_wGCw'C Cuts at: 2037 2094 2529 4887 4972 6043 2037 Size: 57 435 2358 85 1071 2820 Fragments arranged by size: 2820 2358 1071 435 85 57 BsmI GAATG_Cn' Cuts at: 2595 4337 4430 2595 Size: 1742 93 4991 Fragments arranged by size: 4991 1742 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2526 2598 2526 Size: 72 6754 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2037 2094 2529 2565 3291 3662 3797 Size: 399 247 57 435 36 726 371 135 Cuts at: 3797 3853 4118 4145 4887 4972 6043 1391 Size: 56 265 27 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 726 435 399 371 265 247 135 85 57 56 36 27 BspEI T'CCGG_A Cuts at: 3855 6489 3855 Size: 2634 4192 BspHI T'CATG_A Cuts at: 4715 6445 4715 Size: 1730 5096 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4181 844 Size: 3337 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2464 2721 3727 4713 1394 Size: 429 641 257 1006 986 3507 Fragments arranged by size: 3507 1006 986 641 429 257 BsrDI GCAATG_nn' Cuts at: 65 1248 2854 5321 5495 65 Size: 1183 1606 2467 174 1396 Fragments arranged by size: 2467 1606 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2252 2802 3648 5470 5657 1700 Size: 110 442 550 846 1822 187 2869 Fragments arranged by size: 2869 1822 846 550 442 187 110 BsrGI T'GTAC_A Cuts at: 96 2481 3771 96 Size: 2385 1290 3151 Fragments arranged by size: 3151 2385 1290 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6826 BssSI C'ACGA_G Cuts at: 3194 4886 5898 3194 Size: 1692 1012 4122 Fragments arranged by size: 4122 1692 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6826 BstBI TT'CG_AA Cuts at: 5630 5630 Size: 6826 BstEII G'GTnAC_C Cuts at: 1184 3841 1184 Size: 2657 4169 BstXI CCAn_nnnn'nTGG Cuts at: 3952 3952 Size: 6826 BstYI r'GATC_y Cuts at: 1972 2376 3931 4156 4523 4908 4925 6377 6463 Size: 404 1555 225 367 385 17 1452 86 Cuts at: 6463 6475 6821 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1555 1452 404 385 367 346 225 86 17 12 Bsu36I CC'TnA_GG Cuts at: 4119 4119 Size: 6826 BtgI C'CryG_G Cuts at: 513 1065 2294 2402 2778 2913 3100 3356 3387 Size: 552 1229 108 376 135 187 256 31 Cuts at: 3387 513 Size: 3952 Fragments arranged by size: 3952 1229 552 376 256 187 135 108 31 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3559 487 Size: 683 56 2333 3754 Fragments arranged by size: 3754 2333 683 56 BtsI GCAGTG_nn' Cuts at: 3495 4118 4342 5136 5156 3495 Size: 623 224 794 20 5165 Fragments arranged by size: 5165 794 623 224 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6826 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6791 DraI TTT'AAA Cuts at: 4130 4130 Size: 6826 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5833 809 Size: 507 4517 1802 Fragments arranged by size: 4517 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2781 3214 3513 Size: 54 1425 50 275 18 951 433 299 Cuts at: 3513 3650 4273 5162 6791 8 Size: 137 623 889 1629 43 Fragments arranged by size: 1629 1425 951 889 623 433 299 275 137 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3650 4273 1537 Size: 275 1838 623 4090 Fragments arranged by size: 4090 1838 623 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2099 2984 4756 1353 Size: 746 885 1772 3423 Fragments arranged by size: 3423 1772 885 746 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2893 5413 5787 5933 387 Size: 1064 138 1304 2520 374 146 1280 Fragments arranged by size: 2520 1304 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2121 2210 2297 3922 4889 5486 6273 Size: 393 489 89 87 1625 967 597 787 Cuts at: 6273 6541 1239 Size: 268 1524 Fragments arranged by size: 1625 1524 967 787 597 489 393 268 89 87 EcoNI CCTnn'n_nnAGG Cuts at: 2642 3068 2642 Size: 426 6400 EcoO109I rG'GnC_Cy Cuts at: 2207 2305 3287 3288 3464 3488 3658 3659 3849 Size: 98 982 1 176 24 170 1 190 Cuts at: 3849 3850 2207 Size: 1 5183 Fragments arranged by size: 5183 982 190 176 170 98 24 1 1 1 EcoRI G'AATT_C Cuts at: 4135 4513 4135 Size: 378 6448 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3178 3760 3178 Size: 582 6244 FspI TGC'GCA Cuts at: 5332 5332 Size: 6826 HaeII r_GCGC'y Cuts at: 1678 2077 2271 3115 3257 3445 3760 3898 3945 Size: 399 194 844 142 188 315 138 47 Cuts at: 3945 5973 1678 Size: 2028 2531 Fragments arranged by size: 2531 2028 844 399 315 194 188 142 138 47 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2101 2133 2183 2215 2618 Size: 32 135 32 529 32 50 32 403 Cuts at: 2618 2650 2735 2767 2909 2941 3224 3256 3916 Size: 32 85 32 142 32 283 32 660 Cuts at: 3916 3948 5467 5499 5541 5573 1373 Size: 32 1519 32 42 32 2626 Fragments arranged by size: 2626 1519 660 529 403 283 142 135 85 50 42 32 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4170 4416 669 Size: 988 281 2232 246 3079 Fragments arranged by size: 3079 2232 988 281 246 HindIII A'AGCT_T Cuts at: 748 3737 748 Size: 2989 3837 HpaI GTT'AAC Cuts at: 4416 4416 Size: 6826 KasI G'GCGC_C Cuts at: 3111 3756 3894 3111 Size: 645 138 6043 Fragments arranged by size: 6043 645 138 KpnI G_GTAC'C Cuts at: 4151 4151 Size: 6826 MfeI C'AATT_G Cuts at: 4425 4425 Size: 6826 MluI A'CGCG_T Cuts at: 6702 6702 Size: 6826 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2684 2778 2952 3389 5940 6124 1305 Size: 1379 94 174 437 2551 184 2007 Fragments arranged by size: 2551 2007 1379 437 184 174 94 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5004 Fragments arranged by size: 5004 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2087 2171 2477 3330 3521 4784 5143 Size: 648 921 84 306 853 191 1263 359 Cuts at: 5143 5302 518 Size: 159 2042 Fragments arranged by size: 2042 1263 921 853 648 359 306 191 159 84 MspA1I CmG'CkG Cuts at: 1606 2134 2404 2940 3102 3452 3476 3595 3888 Size: 528 270 536 162 350 24 119 293 Cuts at: 3888 4242 4919 6067 6312 1606 Size: 354 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 536 528 354 350 293 270 245 162 119 24 NaeI GCC'GGC Cuts at: 3650 3650 Size: 6826 NarI GG'CG_CC Cuts at: 3112 3757 3895 3112 Size: 645 138 6043 Fragments arranged by size: 6043 645 138 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 6274 NdeI CA'TA_TG Cuts at: 387 387 Size: 6826 NgoMIV G'CCGG_C Cuts at: 3648 3648 Size: 6826 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6826 NotI GC'GGCC_GC Cuts at: 4273 4273 Size: 6826 NspI r_CATG'y Cuts at: 1132 1229 4184 5729 1132 Size: 97 2955 1545 2229 Fragments arranged by size: 2955 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 2638 1348 Size: 1290 5536 PciI A'CATG_T Cuts at: 5725 5725 Size: 6826 PflMI CCAn_nnn'nTGG Cuts at: 1108 3223 1108 Size: 2115 4711 PfoI T'CCnGG_A Cuts at: 4055 4055 Size: 6826 PmeI GTTT'AAAC Cuts at: 4130 4130 Size: 6826 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4895 4927 6408 6440 4895 Size: 32 1481 32 5281 Fragments arranged by size: 5281 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2305 3464 3488 2305 Size: 1159 24 5643 Fragments arranged by size: 5643 1159 24 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6826 PsiI TTA'TAA Cuts at: 4396 4396 Size: 6826 PspOMI G'GGCC_C Cuts at: 3287 3658 3793 3849 3287 Size: 371 135 56 6264 Fragments arranged by size: 6264 371 135 56 PstI C_TGCA'G Cuts at: 830 4113 4178 830 Size: 3283 65 3478 Fragments arranged by size: 3478 3283 65 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6826 PvuII CAG'CTG Cuts at: 2134 3452 3476 3595 2134 Size: 1318 24 119 5365 Fragments arranged by size: 5365 1318 119 24 SacII CC_GC'GG Cuts at: 2405 3103 2405 Size: 698 6128 SalI G'TCGA_C Cuts at: 1936 4168 1936 Size: 2232 4594 SanDI GG'GwC_CC Cuts at: 2305 2305 Size: 6826 SapI GCTCTTCn'nnn_ Cuts at: 2099 2984 2099 Size: 885 5941 SbfI CC_TGCA'GG Cuts at: 4178 4178 Size: 6826 ScaI AGT'ACT Cuts at: 3077 3620 5074 6786 3077 Size: 543 1454 1712 3117 Fragments arranged by size: 3117 1712 1454 543 SexAI A'CCwGG_T Cuts at: 4071 4071 Size: 6826 SfcI C'TryA_G Cuts at: 826 1045 4109 4174 5309 5990 6181 826 Size: 219 3064 65 1135 681 191 1471 Fragments arranged by size: 3064 1471 1135 681 219 191 65 SfoI GGC'GCC Cuts at: 3113 3758 3896 3113 Size: 645 138 6043 Fragments arranged by size: 6043 645 138 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6826 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6826 SmaI CCC'GGG Cuts at: 1872 2840 3970 4153 1872 Size: 968 1130 183 4545 Fragments arranged by size: 4545 1130 968 183 SmlI C'TyrA_G Cuts at: 820 1017 1943 2345 4930 5831 6093 820 Size: 197 926 402 2585 901 262 1553 Fragments arranged by size: 2585 1553 926 901 402 262 197 SnaBI TAC'GTA Cuts at: 493 4617 493 Size: 4124 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6826 SphI G_CATG'C Cuts at: 1132 4184 1132 Size: 3052 3774 SspI AAT'ATT Cuts at: 5 52 4750 5 Size: 47 4698 2081 Fragments arranged by size: 4698 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6826 StyI C'CwwG_G Cuts at: 513 1065 2887 3175 4265 513 Size: 552 1822 288 1090 3074 Fragments arranged by size: 3074 1822 1090 552 288 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3700 3757 4853 4870 5023 5208 5653 Size: 1255 2120 57 1096 17 153 185 445 Cuts at: 5653 325 Size: 1498 Fragments arranged by size: 2120 1498 1255 1096 445 185 153 57 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2481 3075 3618 Size: 274 80 33 51 1443 504 594 543 Cuts at: 3618 3771 5072 6784 96 Size: 153 1301 1712 138 Fragments arranged by size: 1712 1443 1301 594 543 504 274 153 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3816 3848 6786 6818 3816 Size: 32 2938 32 3824 Fragments arranged by size: 3824 2938 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2501 3710 1316 Size: 1185 1209 4432 Fragments arranged by size: 4432 1209 1185 XbaI T'CTAG_A Cuts at: 4162 4162 Size: 6826 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6826 XmaI C'CCGG_G Cuts at: 1870 2838 3968 4151 1870 Size: 968 1130 183 4545 Fragments arranged by size: 4545 1130 968 183 XmnI GAAnn'nnTTC Cuts at: 3563 3740 4955 3563 Size: 177 1215 5434 Fragments arranged by size: 5434 1215 177 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3994 4634 276 Size: 53 83 186 936 2460 640 2468 Fragments arranged by size: 2468 2460 936 640 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacII SalI SanDI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AscI AvrII BaeI BfrBI BsiWI BstZ17I DraIII EcoICRI EcoRV FseI FspAI NruI NsiI PacI PmlI PspXI PsrI RsrII SacI SfiI SrfI SwaI XcmI