Restriction map for
pFN21AE1528
##################################
# NO CUT ENZYMES ( 189 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I AlwI AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI AspHI AspI AvaI
AviII AvrII BalI BamHI BanI
BanII BbeI BbrPI BbsI BbvCI
BcgI BciVI BclI BfrI BglI
BglII BlnI BlpI BmyI BpmI
Bpu1102I BpuAI BsaAI BsaBI BsaHI
BsaI BsaWI BseAI BsgI BsiEI
BsiHKAI BsiWI BsmBI BsoBI Bsp1286I
Bsp1407I BspEI BspHI BspLU11I BspMI
BsrBI BsrDI BsrFI BsrGI BsrI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstXI BstYI
BstZ17I Bsu36I Cac8I CelII Cfr10I
CpoI DdeI DpnII DraIII DrdI
DsaI EagI Eam1105I EarI EclXI
Eco47III Eco52I Eco81I EcoNI EcoO65I
EcoRI EcoT14I EcoT22I FbaI FseI
FspI HaeII HgaI Hin1I HindIII
HpaI HphI KasI KpnI Ksp632I
KspI MaeII MamI MboI MfeI
MflI MluI MluNI MroI MscI
MspA1I MunI MvnI MwoI NaeI
NarI NcoI NdeII NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PstI PvuI PvuII RcaI
RsrII SacI SacII SapI Sau3AI
SbfI ScaI SexAI SfaNI SfcI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SphI Sse8387I SspBI
SspI StuI StyI SwaI Tsp45I
Tth111I Van91I XbaI XcmI XhoI
XhoII XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 40 enzymes) #
###################################
AccI 3 GTCGAC GT^[AC][GT]AC
AcsI 172 GAATTT [GA]^AATT[CT]
AfaI 152 GTAC GT^AC
AlwNI 328 CAGTCTCTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 172 GAATTT [GA]^AATT[CT]
BbvI 376 GCAGC GCAGC(8/12)
BfaI 163 CTAG C^TAG
BsaJI 8 CCCCGG C^C[ACGT][ACGT]GG
BseRI 18 GAGGAG GAGGAG(10/8)
BsmAI 330 GTCTC GTCTC(1/5)
BsmFI 133 GGGAC GGGAC(10/14)
BsmI 108 GAATGC GAATGC(1/-1)
BspDI 281 ATCGAT AT^CGAT
BstNI 372 CCAGG CC^[AT]GG
CfoI 77 GCGC GCG^C
ClaI 281 ATCGAT AT^CGAT
DraI 157 TTTAAA TTT^AAA
DraII 53 AGGACCC [GA]G^G[ACGT]CC[CT]
EaeI 425 CGGCCA [CT]^GGCC[GA]
EcoO109I 53 AGGACCC [GA]G^G[ACGT]CC[CT]
EcoRII 372 CCAGG ^CC[AT]GG
EcoRV 268 GATATC GAT^ATC
FauI 30 GCGGG CCCGC(4/6)
Fnu4HI 376 GCAGC GC^[ACGT]GC
HhaI 77 GCGC GCG^C
HinP1I 77 GCGC G^CGC
HincII 3 GTCGAC GT[CT]^[GA]AC
HindII 3 GTCGAC GT[CT]^[GA]AC
ItaI 376 GCAGC GC^[ACGT]GC
MaeI 163 CTAG C^TAG
MaeIII 45 GTTAC ^GT[ACGT]AC
MslI 232 CATCCAAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvaI 372 CCAGG CC^[AT]GG
NlaIV 54 GGACCC GG[ACGT]^[ACGT]CC
PleI 288 GAGTC GAGTC(4/5)
PpuMI 53 AGGACCC [GA]G^G[AT]CC[CT]
RsaI 152 GTAC GT^AC
SalI 3 GTCGAC G^TCGAC
SpeI 162 ACTAGT A^CTAGT
TseI 376 GCAGC G^C[AT]GC
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AciI 30 GCGG CCGC(-3/-1)
42 GCGG
AluI 383 AGCT AG^CT
442 AGCT
AvaII 54 GGACC G^G[AT]CC
124 GGACC
BcnI 9 CCCGG CC^[GC]GG
14 CCCGG
BstF5I 232 CATCC GGATG(2/0)
339 GGATG
FokI 232 CATCC GGATG(9/13)
339 GGATG
HaeIII 12 GGCC GG^CC
426 GGCC
HapII 10 CCGG C^CGG
15 CCGG
HpaII 10 CCGG C^CGG
15 CCGG
MseI 158 TTAA T^TAA
316 TTAA
MspI 10 CCGG C^CGG
15 CCGG
NciI 9 CCCGG CC^[GC]GG
14 CCCGG
NdeI 258 CATATG CA^TATG
296 CATATG
NlaIII 27 CATG CATG^
99 CATG
TaqI 4 TCGA T^CGA
282 TCGA
Tru9I 158 TTAA T^TAA
316 TTAA
Tsp509I 173 AATT ^AATT
324 AATT
TspRI 72 CAGTG CA[GC]TG[ACGT][ACGT]^
103 CAGTG
TthHB8I 4 TCGA T^CGA
282 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 10 enzymes) #
#########################################
BsiYI BslI BssKI Cfr13I HinfI
MboII MnlI Sau96I ScrFI TfiI