Restriction map for
pFN21AB6321
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# NO CUT ENZYMES ( 177 enzymes) #
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AatII Acc65I AccI AccII AccIII
AciI AclI AcyI AflII AflIII
AgeI AhdI Alw44I AlwI AlwNI
AocI ApaI ApaLI AscI AseI
AsnI Asp700I Asp718I AspEI AspHI
AspI AvaI AviII AvrII BalI
BamHI BanII BbeI BbrPI BbsI
BbvCI BcgI BciVI BcnI BfrI
BglI BglII BlnI BmyI BpuAI
BsaAI BsaBI BsaHI BsaI BsaWI
BseAI BseRI BsgI BsiEI BsiHKAI
BsiWI BsiYI BslI BsmI BsoBI
Bsp1286I BspDI BspEI BspHI BspLU11I
BspMI BsrBI BsrFI BsrI BssHII
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstXI BstYI BstZ17I Bsu36I
Cfr10I ClaI CpoI DraI DraII
DraIII EaeI EagI Eam1105I EarI
EclXI Eco52I Eco81I EcoNI EcoO109I
EcoO65I EcoRI FauI FseI FspI
HapII Hin1I HincII HindII HpaI
HpaII HphI KasI KpnI Ksp632I
KspI MamI MfeI MflI MluI
MluNI MroI MscI MseI MslI
MspI MunI MvnI NaeI NarI
NciI NdeI NgoMIV NheI NotI
NruI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacI SacII SalI
SapI SbfI SexAI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspI StuI
SwaI TfiI Tru9I Tth111I Van91I
XbaI XcmI XhoI XhoII XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AvaII 23 GGACC G^G[AT]CC
BanI 94 GGCACC G^G[CT][GA]CC
BclI 707 TGATCA T^GATCA
BfaI 455 CTAG C^TAG
BlpI 739 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 739 GCTGAGC GC^T[ACGT]AGC
BsmBI 144 GAGACG CGTCTC(1/5)
BsmFI 383 GTCCC GGGAC(10/14)
Bsp1407I 404 TGTACA T^GTACA
BsrGI 404 TGTACA T^GTACA
BssKI 20 CCAGG ^CC[ACGT]GG
BssSI 718 CACGAG CACGAG(-5/-1)
BstNI 20 CCAGG CC^[AT]GG
CelII 739 GCTGAGC GC^T[ACGT]AGC
Cfr13I 23 GGACC G^G[ACGT]CC
DpnII 708 GATC ^GATC
DrdI 552 GACCTTGAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRII 20 CCAGG ^CC[AT]GG
EcoRV 346 GATATC GAT^ATC
EcoT22I 486 ATGCAT ATGCA^T
FbaI 707 TGATCA T^GATCA
HaeIII 480 GGCC GG^CC
HgaI 146 GACGC GACGC(5/10)
HinfI 16 GACTC G^A[ACGT]TC
MaeI 455 CTAG C^TAG
MaeII 381 ACGT A^CGT
MboI 708 GATC ^GATC
MspA1I 509 CAGCTG C[AC]G^C[GT]G
MvaI 20 CCAGG CC^[AT]GG
MwoI 70 GCCATGGAAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NdeII 708 GATC ^GATC
NsiI 486 ATGCAT ATGCA^T
PleI 16 GACTC GAGTC(4/5)
PstI 506 CTGCAG CTGCA^G
PvuII 509 CAGCTG CAG^CTG
Sau3AI 708 GATC ^GATC
Sau96I 23 GGACC G^G[ACGT]CC
ScaI 585 AGTACT AGT^ACT
ScrFI 20 CCAGG CC^[ACGT]GG
SfaNI 244 GATGC GCATC(5/9)
SfcI 506 CTGCAG C^T[GA][CT]AG
SspBI 404 TGTACA T^GTACA
TaqI 6 TCGA T^CGA
Tsp45I 449 GTGAC ^GT[GC]AC
TspRI 222 CAGTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 6 TCGA T^CGA
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# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AcsI 107 GAATTT [GA]^AATT[CT]
191 AAATTT
AfaI 405 GTAC GT^AC
586 GTAC
Aor51HI 336 AGCGCT AGC^GCT
502 AGCGCT
ApoI 107 GAATTT [GA]^AATT[CT]
191 AAATTT
BpmI 18 CTCCAG CTGGAG(16/14)
38 CTCCAG
BsaJI 71 CCATGG C^C[ACGT][ACGT]GG
599 CCATGG
BsmAI 144 GAGAC GTCTC(1/5)
3 GTCTC
BsrDI 276 CATTGC GCAATG(2/0)
398 CATTGC
CfoI 337 GCGC GCG^C
503 GCGC
DdeI 268 CTAAG C^T[ACGT]AG
740 CTGAG
DsaI 71 CCATGG C^C[GA][CT]GG
599 CCATGG
Eco47III 336 AGCGCT AGC^GCT
502 AGCGCT
EcoT14I 71 CCATGG C^C[AT][AT]GG
599 CCATGG
HaeII 336 AGCGCT [GA]GCGC^[CT]
502 AGCGCT
HhaI 337 GCGC GCG^C
503 GCGC
HinP1I 337 GCGC G^CGC
503 GCGC
HindIII 160 AAGCTT A^AGCTT
356 AAGCTT
MaeIII 331 GTTAC ^GT[ACGT]AC
449 GTGAC
NcoI 71 CCATGG C^CATGG
599 CCATGG
NlaIV 94 GGCACC GG[ACGT]^[ACGT]CC
306 GGAGCC
NspI 87 ACATGC [GA]CATG^[CT]
484 ACATGC
RsaI 405 GTAC GT^AC
586 GTAC
StyI 71 CCATGG C^C[AT][AT]GG
599 CCATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 12 enzymes) #
#########################################
AluI BbvI BstF5I Cac8I Fnu4HI
FokI ItaI MboII MnlI NlaIII
TseI Tsp509I