Restriction map for
pFN21AB6916
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# NO CUT ENZYMES ( 179 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcyI AfaI AflII AflIII AgeI
AhdI Alw44I AlwNI AocI ApaI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI AspHI AspI AvaI
AvaII AviII AvrII BalI BamHI
BanI BanII BbeI BbrPI BbsI
BbvCI BcgI BfrI BglI BglII
BlnI BlpI Bpu1102I BpuAI BsaAI
BsaBI BsaHI BsaI BsaWI BseAI
BsgI BsiEI BsiHKAI BsiWI BsmAI
BsmBI BsmFI BsmI BsoBI Bsp1407I
BspDI BspEI BspHI BspLU11I BspMI
BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstPI
BstUI BstXI Bsu36I CelII Cfr10I
ClaI CpoI DraII DraIII DrdI
DsaI EaeI EagI Eam1105I EarI
EclXI Eco52I Eco81I EcoNI EcoO109I
EcoO65I EcoRI EcoRV EcoT14I EcoT22I
FseI FspI HgaI Hin1I HincII
HindII HindIII HpaI HphI KasI
KpnI Ksp632I KspI MaeII MamI
MboII MluI MluNI MroI MscI
MslI MvnI NaeI NarI NcoI
NgoMIV NheI NlaIV NotI NruI
NsiI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
RcaI RsaI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI StyI SwaI
TspRI Tth111I Van91I XbaI XcmI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AccI 409 GTATAC GT^[AC][GT]AC
AcsI 205 GAATTT [GA]^AATT[CT]
AlwI 523 GGATC GGATC(4/5)
Aor51HI 99 AGCGCT AGC^GCT
ApoI 205 GAATTT [GA]^AATT[CT]
BciVI 556 GTATCC GTATCC(6/5)
BclI 57 TGATCA T^GATCA
BcnI 165 CCCGG CC^[GC]GG
BmyI 6 GGGCAC G[AGT]GC[ACT]^C
BpmI 23 CTGGAG CTGGAG(16/14)
BsiYI 274 CCCTCCTCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 274 CCCTCCTCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
Bsp1286I 6 GGGCAC G[AGT]GC[ACT]^C
Bst1107I 409 GTATAC GTA^TAC
BstYI 523 GGATCT [GA]^GATC[CT]
BstZ17I 409 GTATAC GTA^TAC
Cac8I 4 GCGGGC GC[ACGT]^[ACGT]GC
Cfr13I 333 GGCCC G^G[ACGT]CC
DraI 486 TTTAAA TTT^AAA
Eco47III 99 AGCGCT AGC^GCT
FbaI 57 TGATCA T^GATCA
HaeII 99 AGCGCT [GA]GCGC^[CT]
HaeIII 333 GGCC GG^CC
MaeIII 381 GTCAC ^GT[ACGT]AC
MfeI 233 CAATTG C^AATTG
MflI 523 GGATCT [GA]^GATC[CT]
MspA1I 518 CAGCTG C[AC]G^C[GT]G
MunI 233 CAATTG C^AATTG
MwoI 91 GCGGCTGCAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NciI 165 CCCGG CC^[GC]GG
NdeI 430 CATATG CA^TATG
NspI 506 ACATGC [GA]CATG^[CT]
PleI 544 GAGTC GAGTC(4/5)
PstI 95 CTGCAG CTGCA^G
PvuII 518 CAGCTG CAG^CTG
Sau96I 333 GGCCC G^G[ACGT]CC
SfcI 95 CTGCAG C^T[GA][CT]AG
TaqI 225 TCGA T^CGA
TfiI 366 GAATC G^A[AT]TC
Tsp45I 381 GTCAC ^GT[GC]AC
TthHB8I 225 TCGA T^CGA
XhoII 523 GGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 16 enzymes) #
###################################
BfaI 424 CTAG C^TAG
604 CTAG
BseRI 276 CTCCTC GAGGAG(10/8)
109 GAGGAG
CfoI 43 GCGC GCG^C
100 GCGC
DpnII 58 GATC ^GATC
524 GATC
FauI 4 GCGGG CCCGC(4/6)
84 GCGGG
HapII 166 CCGG C^CGG
663 CCGG
HhaI 43 GCGC GCG^C
100 GCGC
HinP1I 43 GCGC G^CGC
100 GCGC
HinfI 366 GAATC G^A[ACGT]TC
544 GAGTC
HpaII 166 CCGG C^CGG
663 CCGG
MaeI 424 CTAG C^TAG
604 CTAG
MboI 58 GATC ^GATC
524 GATC
MspI 166 CCGG C^CGG
663 CCGG
NdeII 58 GATC ^GATC
524 GATC
Sau3AI 58 GATC ^GATC
524 GATC
SfaNI 40 GATGC GCATC(5/9)
345 GCATC
#########################################
# 3 OR MORE CUT ENZYMES ( 21 enzymes) #
#########################################
AciI AluI BbvI BsaJI BsrI
BssKI BstF5I BstNI DdeI EcoRII
Fnu4HI FokI ItaI MnlI MseI
MvaI NlaIII ScrFI Tru9I TseI
Tsp509I