Restriction map for pFN21AB6916
 (Circular) (Six-base) MAPSORT of: pFN21AB6916.seq  Check: 525  from: 1  to: 5380



pFN21AB6916  5380 bp

 With 182 enzymes: SgfI * 

                        October 20, 2009 10:41  ..

AatII G_ACGT'C

Cuts at:    278     331     414     600    1536    3190     278 
   Size:         53      83     186     936    1654    2468

  Fragments arranged by size: 

                2468    1654     936     186      83      53
 
AccI GT'mk_AC

Cuts at:   1937    2415    2723    1937 
   Size:        478     308    4594

  Fragments arranged by size: 

                4594     478     308
 
Acc65I G'GTAC_C

Cuts at:   2701    2701 
   Size:       5380
 
AclI AA'CG_TT

Cuts at:   1563    3507    3880    1563 
   Size:       1944     373    3063

  Fragments arranged by size: 

                3063    1944     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1632    2447    3443    4827    5095    1632 
   Size:        815     996    1384     268    1917

  Fragments arranged by size: 

                1917    1384     996     815     268
 
AfeI AGC'GCT

Cuts at:   2106    2106 
   Size:       5380
 
AflII C'TTAA_G

Cuts at:    820    1017     820 
   Size:        197    5183
 
AflIII A'CryG_T

Cuts at:   1203    4279    5256    1203 
   Size:       3076     977    1327

  Fragments arranged by size: 

                3076    1327     977
 
AgeI A'CCGG_T

Cuts at:   4211    4211 
   Size:       5380
 
AhdI GACnn_n'nnGTC

Cuts at:   4109    4109 
   Size:       5380
 
AleI CACnn'nnGTG

Cuts at:   1166    1166 
   Size:       5380
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1184    1184 
   Size:       5380
 
AlwNI CAG_nnn'CTG

Cuts at:   3123    4695    3123 
   Size:       1572    3808
 
ApaLI G'TGCA_C

Cuts at:   3437    4593    3437 
   Size:       1156    4224
 
ApoI r'AATT_y

Cuts at:   1493    2210    2689    2915    3067    5176    1493 
   Size:        717     479     226     152    2109    1697

  Fragments arranged by size: 

                2109    1697     717     479     226     152
 
AseI AT'TA_AT

Cuts at:    160    1032    3934     160 
   Size:        872    2902    1606

  Fragments arranged by size: 

                2902    1606     872
 
AsiSI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5380
 
AvaI C'yCGr_G

Cuts at:   1870    1943    2705    1870 
   Size:         73     762    4545

  Fragments arranged by size: 

                4545     762      73
 
BamHI G'GATC_C

Cuts at:   2710    3077    2710 
   Size:        367    5013
 
BanI G'GyrC_C

Cuts at:    618     943    1160    1787    2701    4156     618 
   Size:        325     217     627     914    1455    1842

  Fragments arranged by size: 

                1842    1455     914     627     325     217
 
BanII G_rGCy'C

Cuts at:   1391    2699    1391 
   Size:       1308    4072
 
BbsI GAAGACnn'nnnn_

Cuts at:    928    1899     928 
   Size:        971    4409
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1903    1937    3571    3605    1903 
   Size:         34    1634      34    3678

  Fragments arranged by size: 

                3678    1634      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   2572    3272    4482    2572 
   Size:        700    1210    3470

  Fragments arranged by size: 

                3470    1210     700
 
BclI T'GATC_A

Cuts at:   1548    2062    1548 
   Size:        514    4866
 
BglI GCCn_nnn'nGGC

Cuts at:    136     243     365     436    1067    1672    3991     136 
   Size:        107     122      71     631     605    2319    1525

  Fragments arranged by size: 

                2319    1525     631     605     122     107      71
 
BglII A'GATC_T

Cuts at:   5375    5375 
   Size:       5380
 
BlpI GC'TnA_GC

Cuts at:   2797    2797 
   Size:       5380
 
Bme1580I G_kGCm'C

Cuts at:   1790    2015    3441    4597    1790 
   Size:        225    1426    1156    2573

  Fragments arranged by size: 

                2573    1426    1156     225
 
BmgBI CAC'GTC

Cuts at:   1318    1318 
   Size:       5380
 
BmrI ACTGGGnnnn_n'

Cuts at:    442     858     911     994    1393    1417    2335    3111    4069 
   Size:        416      53      83     399      24     918     776     958

Cuts at:   4069     442 
   Size:       1753

  Fragments arranged by size: 

                1753     958     918     776     416     399      83      53
                  24
 
BmtI G_CTAG'C

Cuts at:   1056    1056 
   Size:       5380
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    2049    4040    1239 
   Size:        810    1991    2579

  Fragments arranged by size: 

                2579    1991     810
 
Bpu10I CC'TnA_GC

Cuts at:   2153    2153 
   Size:       5380
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   3505    4370    4668    3505 
   Size:        865     298    4217

  Fragments arranged by size: 

                4217     865     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    882    1499    4043     882 
   Size:        617    2544    2219

  Fragments arranged by size: 

                2544    2219     617
 
BsaAI yAC'GTr

Cuts at:    493    1204    3171     493 
   Size:        711    1967    2702

  Fragments arranged by size: 

                2702    1967     711
 
BsaBI GATnn'nnATC

Cuts at:   5374    5374 
   Size:       5380
 
BsaHI Gr'CG_yC

Cuts at:    275     328     411     597    1533    3187    3569    4204     275 
   Size:         53      83     186     936    1654     382     635    1451

  Fragments arranged by size: 

                1654    1451     936     635     382     186      83      53
            
 
BsaWI w'CCGG_w

Cuts at:   3813    4211    4485    4632    5043    3813 
   Size:        398     274     147     411    4150

  Fragments arranged by size: 

                4150     411     398     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1178    1208    1373    1403    2022    2052    1178 
   Size:         30     165      30     619      30    4506

  Fragments arranged by size: 

                4506     619     165      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1186    2129    2272    1186 
   Size:        943     143    4294

  Fragments arranged by size: 

                4294     943     143
 
BseYI C'CCAG_C

Cuts at:   4583    5079    4583 
   Size:        496    4884
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1736    1163 
   Size:        573    4807
 
BsiEI CG_ry'CG

Cuts at:   1540    1815    2001    2830    3591    4619    1540 
   Size:        275     186     829     761    1028    2301

  Fragments arranged by size: 

                2301    1028     829     761     275     186
 
BsiHKAI G_wGCw'C

Cuts at:   3441    3526    4597    3441 
   Size:         85    1071    4224

  Fragments arranged by size: 

                4224    1071      85
 
BsmI GAATG_Cn'

Cuts at:   2891    2984    2891 
   Size:         93    5287
 
Bsp1286I G_dGCh'C

Cuts at:   1391    1790    2015    2699    3441    3526    4597    1391 
   Size:        399     225     684     742      85    1071    2174

  Fragments arranged by size: 

                2174    1071     742     684     399     225      85
 
BspEI T'CCGG_A

Cuts at:   5043    5043 
   Size:       5380
 
BspHI T'CATG_A

Cuts at:   3269    4999    3269 
   Size:       1730    3650
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    844    2735     844 
   Size:       1891    3489
 
BsrBI CCG'CTC

Cuts at:   1394    1823    3267    1394 
   Size:        429    1444    3507

  Fragments arranged by size: 

                3507    1444     429
 
BsrDI GCAATG_nn'

Cuts at:     65    1248    3875    4049      65 
   Size:       1183    2627     174    1396

  Fragments arranged by size: 

                2627    1396    1183     174
 
BsrFI r'CCGG_y

Cuts at:   1700    1810    4024    4211    1700 
   Size:        110    2214     187    2869

  Fragments arranged by size: 

                2869    2214     187     110
 
BsrGI T'GTAC_A

Cuts at:     96      96 
   Size:       5380
 
BssHII G'CGCG_C

Cuts at:   1925    1925 
   Size:       5380
 
BssSI C'ACGA_G

Cuts at:   3440    4452    3440 
   Size:       1012    4368
 
BstAPI GCAn_nnn'nTGC

Cuts at:    794     794 
   Size:       5380
 
BstBI TT'CG_AA

Cuts at:   4184    4184 
   Size:       5380
 
BstEII G'GTnAC_C

Cuts at:   1184    1184 
   Size:       5380
 
BstYI r'GATC_y

Cuts at:   1972    2528    2710    3077    3462    3479    4931    5017    5029 
   Size:        556     182     367     385      17    1452      86      12

Cuts at:   5029    5375    1972 
   Size:        346    1977

  Fragments arranged by size: 

                1977    1452     556     385     367     346     182      86
                  17      12
 
BstZ17I GTA'TAC

Cuts at:   2416    2416 
   Size:       5380
 
BtgI C'CryG_G

Cuts at:    513    1065    2004     513 
   Size:        552     939    3889

  Fragments arranged by size: 

                3889     939     552
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    487    1170    1226     487 
   Size:        683      56    4641

  Fragments arranged by size: 

                4641     683      56
 
BtsI GCAGTG_nn'

Cuts at:   2896    3690    3710    2896 
   Size:        794      20    4566

  Fragments arranged by size: 

                4566     794      20
 
ClaI AT'CG_AT

Cuts at:   1554    1554 
   Size:       5380
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    529     564     529 
   Size:         35    5345
 
DraI TTT'AAA

Cuts at:   2493    2684    2493 
   Size:        191    5189
 
DrdI GACnn_nn'nnGTC

Cuts at:    809    1316    4387     809 
   Size:        507    3071    1802

  Fragments arranged by size: 

                3071    1802     507
 
EaeI y'GGCC_r

Cuts at:      8      62    1487    1537    1812    1830    2827    3716    5345 
   Size:         54    1425      50     275      18     997     889    1629

Cuts at:   5345       8 
   Size:         43

  Fragments arranged by size: 

                1629    1425     997     889     275      54      50      43
                  18
 
EagI C'GGCC_G

Cuts at:   1537    1812    2827    1537 
   Size:        275    1015    4090

  Fragments arranged by size: 

                4090    1015     275
 
EarI CTCTTCn'nnn_

Cuts at:   1353    3310    1353 
   Size:       1957    3423
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    387    1451    1589    3967    4341    4487     387 
   Size:       1064     138    2378     374     146    1280

  Fragments arranged by size: 

                2378    1280    1064     374     146     138
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    1632    2049    2447    3443    4040    4827    5095    1239 
   Size:        393     417     398     996     597     787     268    1524

  Fragments arranged by size: 

                1524     996     787     597     417     398     393     268
            
 
EcoRI G'AATT_C

Cuts at:   2689    3067    2689 
   Size:        378    5002
 
FspI TGC'GCA

Cuts at:   3886    3886 
   Size:       5380
 
HaeII r_GCGC'y

Cuts at:   1678    2108    4527    1678 
   Size:        430    2419    2531

  Fragments arranged by size: 

                2531    2419     430
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1373    1405    1540    1572    4021    4053    4095    4127    1373 
   Size:         32     135      32    2449      32      42      32    2626

  Fragments arranged by size: 

                2626    2449     135      42      32      32      32      32
            
 
HincII GTy'rAC

Cuts at:    669    1657    1938    2724    2970     669 
   Size:        988     281     786     246    3079

  Fragments arranged by size: 

                3079     988     786     281     246
 
HindIII A'AGCT_T

Cuts at:    748     748 
   Size:       5380
 
HpaI GTT'AAC

Cuts at:   2970    2970 
   Size:       5380
 
KpnI G_GTAC'C

Cuts at:   2705    2705 
   Size:       5380
 
MfeI C'AATT_G

Cuts at:   2238    2979    2238 
   Size:        741    4639
 
MluI A'CGCG_T

Cuts at:   5256    5256 
   Size:       5380
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1305    4494    4678    1305 
   Size:       3189     184    2007

  Fragments arranged by size: 

                3189    2007     184
 
MscI TGG'CCA

Cuts at:     10      64    1489    1832      10 
   Size:         54    1425     343    3558

  Fragments arranged by size: 

                3558    1425     343      54
 
MslI CAynn'nnrTG

Cuts at:    518    1166    3338    3697    3856     518 
   Size:        648    2172     359     159    2042

  Fragments arranged by size: 

                2172    2042     648     359     159
 
MspA1I CmG'CkG

Cuts at:   1606    2525    2796    3473    4621    4866    1606 
   Size:        919     271     677    1148     245    2120

  Fragments arranged by size: 

                2120    1148     919     677     271     245
 
NcoI C'CATG_G

Cuts at:    513    1065    2004     513 
   Size:        552     939    3889

  Fragments arranged by size: 

                3889     939     552
 
NdeI CA'TA_TG

Cuts at:    387    2436     387 
   Size:       2049    3331
 
NheI G'CTAG_C

Cuts at:   1052    1052 
   Size:       5380
 
NotI GC'GGCC_GC

Cuts at:   2827    2827 
   Size:       5380
 
NspI r_CATG'y

Cuts at:   1132    1229    2515    2738    4283    1132 
   Size:         97    1286     223    1545    2229

  Fragments arranged by size: 

                2229    1545    1286     223      97
 
PasI CC'CwG_GG

Cuts at:   1348    1348 
   Size:       5380
 
PciI A'CATG_T

Cuts at:   4279    4279 
   Size:       5380
 
PflMI CCAn_nnn'nTGG

Cuts at:   1108    1108 
   Size:       5380
 
PmeI GTTT'AAAC

Cuts at:   2684    2684 
   Size:       5380
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3449    3481    4962    4994    3449 
   Size:         32    1481      32    3835

  Fragments arranged by size: 

                3835    1481      32      32
 
PshAI GACnn'nnGTC

Cuts at:   1613    1613 
   Size:       5380
 
PsiI TTA'TAA

Cuts at:   2950    2950 
   Size:       5380
 
PstI C_TGCA'G

Cuts at:    830    2104    2732     830 
   Size:       1274     628    3478

  Fragments arranged by size: 

                3478    1274     628
 
PvuI CG_AT'CG

Cuts at:   2001    2001 
   Size:       5380
 
PvuII CAG'CTG

Cuts at:   2525    2525 
   Size:       5380
 
SalI G'TCGA_C

Cuts at:   1936    2722    1936 
   Size:        786    4594
 
SbfI CC_TGCA'GG

Cuts at:   2732    2732 
   Size:       5380
 
ScaI AGT'ACT

Cuts at:   3628    5340    3628 
   Size:       1712    3668
 
SfcI C'TryA_G

Cuts at:    826    1045    2100    2728    3863    4544    4735     826 
   Size:        219    1055     628    1135     681     191    1471

  Fragments arranged by size: 

                1471    1135    1055     681     628     219     191
 
SgfI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5380
 
SgrAI Cr'CCGG_yG

Cuts at:   1700    1700 
   Size:       5380
 
SmaI CCC'GGG

Cuts at:   1872    2707    1872 
   Size:        835    4545
 
SmlI C'TyrA_G

Cuts at:    820    1017    1943    3484    4385    4647     820 
   Size:        197     926    1541     901     262    1553

  Fragments arranged by size: 

                1553    1541     926     901     262     197
 
SnaBI TAC'GTA

Cuts at:    493    3171     493 
   Size:       2678    2702
 
SpeI A'CTAG_T

Cuts at:    152     152 
   Size:       5380
 
SphI G_CATG'C

Cuts at:   1132    2738    1132 
   Size:       1606    3774
 
SspI AAT'ATT

Cuts at:      5      52    3304       5 
   Size:         47    3252    2081

  Fragments arranged by size: 

                3252    2081      47
 
StuI AGG'CCT

Cuts at:   1517    1517 
   Size:       5380
 
StyI C'CwwG_G

Cuts at:    513    1065    2004    2819     513 
   Size:        552     939     815    3074

  Fragments arranged by size: 

                3074     939     815     552
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    325    1580    3407    3424    3577    3762    4207     325 
   Size:       1255    1827      17     153     185     445    1498

  Fragments arranged by size: 

                1827    1498    1255     445     185     153      17
 
TatI w'GTAC_w

Cuts at:     96     370     450     483     534    1977    3626    5338      96 
   Size:        274      80      33      51    1443    1649    1712     138

  Fragments arranged by size: 

                1712    1649    1443     274     138      80      51      33
            
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   2022    2054    5340    5372    2022 
   Size:         32    3286      32    2030

  Fragments arranged by size: 

                3286    2030      32      32
 
Tth111I GACn'n_nGTC

Cuts at:   1316    1316 
   Size:       5380
 
XbaI T'CTAG_A

Cuts at:   2716    2716 
   Size:       5380
 
XhoI C'TCGA_G

Cuts at:   1943    1943 
   Size:       5380
 
XmaI C'CCGG_G

Cuts at:   1870    2705    1870 
   Size:        835    4545
 
XmnI GAAnn'nnTTC

Cuts at:   3509    3509 
   Size:       5380
 
ZraI GAC'GTC

Cuts at:    276     329     412     598    1534    3188     276 
   Size:         53      83     186     936    1654    2468

  Fragments arranged by size: 

                2468    1654     936     186      83      53
 

 Enzymes that do cut:

    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI    AlwNI    ApaLI     ApoI     AseI
    AsiSI     AvaI    BamHI     BanI    BanII     BbsI     BcgI    BciVI
     BclI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI
     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI
    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI Bsp1286I
    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII
    BssSI   BstAPI    BstBI   BstEII    BstYI  BstZ17I     BtgI    BtgZI
     BtsI     ClaI    CspCI     DraI     DrdI     EaeI     EagI     EarI
     EciI  Eco57MI    EcoRI     FspI    HaeII    Hin4I   HincII  HindIII
     HpaI     KpnI     MfeI     MluI     MmeI     MscI     MslI   MspA1I
     NcoI     NdeI     NheI     NotI     NspI     PasI     PciI    PflMI
     PmeI     PpiI    PshAI     PsiI     PstI     PvuI    PvuII     SalI
     SbfI     ScaI     SfcI     SgfI    SgrAI     SmaI     SmlI    SnaBI
     SpeI     SphI     SspI     StuI     StyI    TaqII     TatI     TstI
  Tth111I     XbaI     XhoI     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AarI     AloI     ApaI     AscI    AvrII     BaeI     BbeI    BbvCI
    BfrBI     BplI    BsiWI    BsmBI    BstXI   Bsu36I   DraIII  EcoICRI
    EcoNI EcoO109I    EcoRV     FalI     FseI    FspAI     KasI     NaeI
     NarI   NgoMIV     NruI     NsiI     PacI     PfoI     PmlI    PpuMI
   PspOMI    PspXI     PsrI    RsrII     SacI    SacII    SanDI     SapI
    SexAI     SfiI     SfoI     SrfI     SwaI     XcmI
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