################################## # NO CUT ENZYMES ( 164 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AspHI AspI AvaI AviII AvrII BamHI BanI BanII BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BfrI BlnI BmyI BpuAI BsaAI BsaBI BsaI BsaWI BseAI BsgI BsiEI BsiHKAI BsiWI BsmFI BsmI BsoBI Bsp1286I Bsp1407I BspDI BspEI BspLU11I BspMI BsrBI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstZ17I Bsu36I CfoI Cfr10I ClaI CpoI DraI DraII DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRV EcoT14I EcoT22I FbaI FseI FspI HaeII HhaI HinP1I HincII HindII HindIII HpaI KasI KpnI KspI MamI MfeI MluI MroI MslI MunI MvnI NaeI NarI NcoI NdeI NgoMIV NheI NlaIV NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI PstI PvuI RsrII SacI SacII SalI SapI SbfI ScaI SfcI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI Tth111I Van91I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 47 enzymes) # ################################### AclI 263 AACGTT AA^CGTT AcyI 73 GACGCC G[GA]^CG[CT]C AlwI 130 GATCC GGATC(4/5) AlwNI 424 CAGATACTG CAG[ACGT][ACGT][ACGT]^CTG Asp700I 439 GAACTACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AvaII 32 GGACC G^G[AT]CC BalI 104 TGGCCA TGG^CCA BbvI 51 GCAGC GCAGC(8/12) BcnI 387 CCGGG CC^[GC]GG BglI 661 GCCTCCAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlpI 847 GCTGAGC GC^T[ACGT]AGC Bpu1102I 847 GCTGAGC GC^T[ACGT]AGC BsaHI 73 GACGCC G[GA]^CG[CT]C BsiYI 496 CCATTTGAGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 496 CCATTTGAGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BsmAI 907 GTCTC GTCTC(1/5) BsmBI 906 CGTCTC CGTCTC(1/5) BspHI 150 TCATGA T^CATGA BsrDI 220 GCAATG GCAATG(2/0) BstF5I 603 GGATG GGATG(2/0) CelII 847 GCTGAGC GC^T[ACGT]AGC Cfr13I 32 GGACC G^G[ACGT]CC DsaI 101 CCGTGG C^C[GA][CT]GG EaeI 104 TGGCCA [CT]^GGCC[GA] EarI 876 GAAGAG CTCTTC(1/4) FokI 603 GGATG GGATG(9/13) HapII 387 CCGG C^CGG HgaI 73 GACGC GACGC(5/10) Hin1I 73 GACGCC G[GA]^CG[CT]C HpaII 387 CCGG C^CGG HphI 297 TCACC GGTGA(8/7) Ksp632I 876 GAAGAG CTCTTC(1/4) MluNI 104 TGGCCA TGG^CCA MscI 104 TGGCCA TGG^CCA MspI 387 CCGG C^CGG NciI 387 CCGGG CC^[GC]GG Psp1406I 263 AACGTT AA^CGTT RcaI 150 TCATGA T^CATGA Sau96I 32 GGACC G^G[ACGT]CC SexAI 953 ACCTGGT A^CC[AT]GGT SmlI 142 CTTGAG C^T[CT][GA]AG TaqI 515 TCGA T^CGA TseI 51 GCAGC G^C[AT]GC Tsp45I 735 GTCAC ^GT[GC]AC TspRI 28 CACTG CA[GC]TG[ACGT][ACGT]^ TthHB8I 515 TCGA T^CGA XmnI 439 GAACTACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 18 enzymes) # ################################### AfaI 3 GTAC GT^AC 242 GTAC BglII 229 AGATCT A^GATCT 796 AGATCT BpmI 39 CTCCAG CTGGAG(16/14) 663 CTCCAG BseRI 46 GAGGAG GAGGAG(10/8) 928 GAGGAG BstYI 229 AGATCT [GA]^GATC[CT] 796 AGATCT EcoRI 196 GAATTC G^AATTC 349 GAATTC FauI 527 CCCGC CCCGC(4/6) 66 GCGGG Fnu4HI 24 GCGGC GC^[ACGT]GC 51 GCAGC HaeIII 105 GGCC GG^CC 333 GGCC ItaI 24 GCGGC GC^[ACGT]GC 51 GCAGC MaeIII 735 GTCAC ^GT[ACGT]AC 741 GTTAC MflI 229 AGATCT [GA]^GATC[CT] 796 AGATCT MspA1I 52 CAGCTG C[AC]G^C[GT]G 569 CAGCTG PleI 393 GAGTC GAGTC(4/5) 702 GAGTC PvuII 52 CAGCTG CAG^CTG 569 CAGCTG RsaI 3 GTAC GT^AC 242 GTAC TfiI 546 GAATC G^A[AT]TC 961 GAATC XhoII 229 AGATCT [GA]^GATC[CT] 796 AGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 29 enzymes) # ######################################### AciI AcsI AluI ApoI BfaI BsaJI BsrI BssKI BstNI Cac8I DdeI DpnII EcoRII HinfI MaeI MaeII MboI MboII MnlI MseI MvaI MwoI NdeII NlaIII Sau3AI ScrFI SfaNI Tru9I Tsp509I