(Circular) (Six-base) MAPSORT of: pFN21AE1536.seq Check: 2060 from: 1 to: 5674 pFN21AE1536 5674 bp With 182 enzymes: SgfI * August 16, 2010 14:51 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3484 278 Size: 53 83 186 936 1948 2468 Fragments arranged by size: 2468 1948 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3017 1937 Size: 1080 4594 Acc65I G'GTAC_C Cuts at: 2995 2995 Size: 5674 AclI AA'CG_TT Cuts at: 1563 2269 3801 4174 1563 Size: 706 1532 373 3063 Fragments arranged by size: 3063 1532 706 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2826 3737 5121 5389 1632 Size: 1194 911 1384 268 1917 Fragments arranged by size: 1917 1384 1194 911 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5477 AflIII A'CryG_T Cuts at: 1203 4573 5550 1203 Size: 3370 977 1327 Fragments arranged by size: 3370 1327 977 AgeI A'CCGG_T Cuts at: 4505 4505 Size: 5674 AhdI GACnn_n'nnGTC Cuts at: 4403 4403 Size: 5674 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5674 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5674 AlwNI CAG_nnn'CTG Cuts at: 2434 3417 4989 2434 Size: 983 1572 3119 Fragments arranged by size: 3119 1572 983 ApaLI G'TGCA_C Cuts at: 3731 4887 3731 Size: 1156 4518 ApoI r'AATT_y Cuts at: 1493 2090 2201 2354 2983 3209 3361 5470 1493 Size: 597 111 153 629 226 152 2109 1697 Fragments arranged by size: 2109 1697 629 597 226 153 152 111 AseI AT'TA_AT Cuts at: 160 1032 4228 160 Size: 872 3196 1606 Fragments arranged by size: 3196 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5674 AvaI C'yCGr_G Cuts at: 1870 1943 2999 1870 Size: 73 1056 4545 Fragments arranged by size: 4545 1056 73 BamHI G'GATC_C Cuts at: 3004 3371 3004 Size: 367 5307 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2995 4450 618 Size: 325 217 627 1208 1455 1842 Fragments arranged by size: 1842 1455 1208 627 325 217 BanII G_rGCy'C Cuts at: 1391 2993 1391 Size: 1602 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4703 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3865 3899 1903 Size: 34 1928 34 3678 Fragments arranged by size: 3678 1928 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3566 4776 3566 Size: 1210 4464 BclI T'GATC_A Cuts at: 1548 1548 Size: 5674 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2672 4285 136 Size: 107 122 71 631 605 1000 1613 1525 Fragments arranged by size: 1613 1525 1000 631 605 122 107 71 BglII A'GATC_T Cuts at: 2234 2801 5669 2234 Size: 567 2868 2239 Fragments arranged by size: 2868 2239 567 BlpI GC'TnA_GC Cuts at: 2853 3091 2853 Size: 238 5436 Bme1580I G_kGCm'C Cuts at: 1790 3735 4891 1790 Size: 1945 1156 2573 Fragments arranged by size: 2573 1945 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5674 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3405 4363 442 Size: 416 53 83 399 24 1988 958 1753 Fragments arranged by size: 1988 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5674 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2029 2653 4334 1239 Size: 790 624 1681 2579 Fragments arranged by size: 2579 1681 790 624 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2168 3799 4664 4962 2168 Size: 1631 865 298 2880 Fragments arranged by size: 2880 1631 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4337 882 Size: 617 2838 2219 Fragments arranged by size: 2838 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3465 493 Size: 711 2261 2702 Fragments arranged by size: 2702 2261 711 BsaBI GATnn'nnATC Cuts at: 5668 5668 Size: 5674 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2079 3481 3863 4498 Size: 53 83 186 936 546 1402 382 635 Cuts at: 4498 275 Size: 1451 Fragments arranged by size: 1451 1402 936 635 546 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4107 4505 4779 4926 5337 4107 Size: 398 274 147 411 4444 Fragments arranged by size: 4444 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5449 Fragments arranged by size: 5449 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2066 2948 1186 Size: 880 882 3912 Fragments arranged by size: 3912 882 880 BseYI C'CCAG_C Cuts at: 4877 5373 4877 Size: 496 5178 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5101 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3124 3885 4913 1540 Size: 275 186 1123 761 1028 2301 Fragments arranged by size: 2301 1123 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3735 3820 4891 3735 Size: 85 1071 4518 Fragments arranged by size: 4518 1071 85 BsmI GAATG_Cn' Cuts at: 3185 3278 3185 Size: 93 5581 BsmBI CGTCTCn'nnnn_ Cuts at: 2917 2917 Size: 5674 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2993 3735 3820 4891 1391 Size: 399 1203 742 85 1071 2174 Fragments arranged by size: 2174 1203 1071 742 399 85 BspEI T'CCGG_A Cuts at: 5337 5337 Size: 5674 BspHI T'CATG_A Cuts at: 2155 3563 5293 2155 Size: 1408 1730 2536 Fragments arranged by size: 2536 1730 1408 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3029 844 Size: 2185 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3561 1394 Size: 429 1738 3507 Fragments arranged by size: 3507 1738 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2232 4169 4343 65 Size: 1183 984 1937 174 1396 Fragments arranged by size: 1937 1396 1183 984 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4318 4505 1700 Size: 110 2508 187 2869 Fragments arranged by size: 2869 2508 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5674 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5674 BssSI C'ACGA_G Cuts at: 3734 4746 3734 Size: 1012 4662 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5674 BstBI TT'CG_AA Cuts at: 4478 4478 Size: 5674 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5674 BstYI r'GATC_y Cuts at: 1972 2234 2801 3004 3371 3756 3773 5225 5311 Size: 262 567 203 367 385 17 1452 86 Cuts at: 5311 5323 5669 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 567 385 367 346 262 203 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2106 513 Size: 552 1041 4081 Fragments arranged by size: 4081 1041 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2854 487 Size: 683 56 1628 3307 Fragments arranged by size: 3307 1628 683 56 BtsI GCAGTG_nn' Cuts at: 3190 3984 4004 3190 Size: 794 20 4860 Fragments arranged by size: 4860 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5674 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5639 DraI TTT'AAA Cuts at: 2978 2978 Size: 5674 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4681 809 Size: 507 3365 1802 Fragments arranged by size: 3365 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2109 3121 4010 Size: 54 1425 50 275 18 279 1012 889 Cuts at: 4010 5639 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1012 889 279 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3121 1537 Size: 275 1309 4090 Fragments arranged by size: 4090 1309 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2876 3604 1353 Size: 1523 728 3423 Fragments arranged by size: 3423 1523 728 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4261 4635 4781 387 Size: 1064 138 2672 374 146 1280 Fragments arranged by size: 2672 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2029 2653 2826 3737 4334 5121 5389 Size: 393 397 624 173 911 597 787 268 Cuts at: 5389 1239 Size: 1524 Fragments arranged by size: 1524 911 787 624 597 397 393 268 173 EcoRI G'AATT_C Cuts at: 2201 2354 2983 3361 2201 Size: 153 629 378 4514 Fragments arranged by size: 4514 629 378 153 FspI TGC'GCA Cuts at: 4180 4180 Size: 5674 HaeII r_GCGC'y Cuts at: 1678 4821 1678 Size: 3143 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2521 2553 2897 2929 4315 Size: 32 135 32 949 32 344 32 1386 Cuts at: 4315 4347 4389 4421 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1386 949 344 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3018 3264 669 Size: 988 281 1080 246 3079 Fragments arranged by size: 3079 1080 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5674 HpaI GTT'AAC Cuts at: 3264 3264 Size: 5674 KpnI G_GTAC'C Cuts at: 2999 2999 Size: 5674 MfeI C'AATT_G Cuts at: 3273 3273 Size: 5674 MluI A'CGCG_T Cuts at: 5550 5550 Size: 5674 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2362 2814 4788 4972 1305 Size: 1057 452 1974 184 2007 Fragments arranged by size: 2007 1974 1057 452 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2111 10 Size: 54 1425 343 279 3573 Fragments arranged by size: 3573 1425 343 279 54 MslI CAynn'nnrTG Cuts at: 518 1166 3632 3991 4150 518 Size: 648 2466 359 159 2042 Fragments arranged by size: 2466 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2059 2576 3090 3767 4915 5160 1606 Size: 453 517 514 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 517 514 453 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5122 NdeI CA'TA_TG Cuts at: 387 387 Size: 5674 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5674 NotI GC'GGCC_GC Cuts at: 3121 3121 Size: 5674 NspI r_CATG'y Cuts at: 1132 1229 3032 4577 1132 Size: 97 1803 1545 2229 Fragments arranged by size: 2229 1803 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5674 PciI A'CATG_T Cuts at: 4573 4573 Size: 5674 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5674 PfoI T'CCnGG_A Cuts at: 2391 2391 Size: 5674 PmeI GTTT'AAAC Cuts at: 2978 2978 Size: 5674 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3743 3775 5256 5288 3743 Size: 32 1481 32 4129 Fragments arranged by size: 4129 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5674 PsiI TTA'TAA Cuts at: 3244 3244 Size: 5674 PstI C_TGCA'G Cuts at: 830 3026 830 Size: 2196 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5674 PvuII CAG'CTG Cuts at: 2059 2576 2059 Size: 517 5157 SalI G'TCGA_C Cuts at: 1936 3016 1936 Size: 1080 4594 SbfI CC_TGCA'GG Cuts at: 3026 3026 Size: 5674 ScaI AGT'ACT Cuts at: 3922 5634 3922 Size: 1712 3962 SexAI A'CCwGG_T Cuts at: 2958 2958 Size: 5674 SfcI C'TryA_G Cuts at: 826 1045 3022 4157 4838 5029 826 Size: 219 1977 1135 681 191 1471 Fragments arranged by size: 1977 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5674 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5674 SmaI CCC'GGG Cuts at: 1872 3001 1872 Size: 1129 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2147 3778 4679 4941 820 Size: 197 926 204 1631 901 262 1553 Fragments arranged by size: 1631 1553 926 901 262 204 197 SnaBI TAC'GTA Cuts at: 493 3465 493 Size: 2972 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5674 SphI G_CATG'C Cuts at: 1132 3032 1132 Size: 1900 3774 SspI AAT'ATT Cuts at: 5 52 3598 5 Size: 47 3546 2081 Fragments arranged by size: 3546 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5674 StyI C'CwwG_G Cuts at: 513 1065 3113 513 Size: 552 2048 3074 Fragments arranged by size: 3074 2048 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2812 3701 3718 3871 4056 4501 325 Size: 1255 1232 889 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1232 889 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3920 5632 96 Size: 274 80 33 51 1443 1943 1712 138 Fragments arranged by size: 1943 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5634 5666 5634 Size: 32 5642 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5674 XbaI T'CTAG_A Cuts at: 3010 3010 Size: 5674 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5674 XmaI C'CCGG_G Cuts at: 1870 2999 1870 Size: 1129 4545 XmnI GAAnn'nnTTC Cuts at: 2448 3803 2448 Size: 1355 4319 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3482 276 Size: 53 83 186 936 1948 2468 Fragments arranged by size: 2468 1948 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SfiI SfoI SrfI SwaI XcmI