################################## # NO CUT ENZYMES ( 187 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcsI AcyI AfaI AflII AflIII AgeI AhdI Alw44I AlwI AlwNI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AviII BalI BamHI BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BcnI BfrI BglI BglII BlpI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BsaWI BseAI BseRI BsiEI BsiWI BsmAI BsmBI BsmFI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstEII BstPI BstUI BstYI BstZ17I Bsu36I Cac8I CelII CfoI Cfr10I ClaI CpoI DpnII DraI DrdI DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FauI FbaI FseI FspI HaeII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI KasI KpnI KspI MaeII MamI MboI MfeI MflI MluI MluNI MroI MscI MspA1I MunI MvnI NaeI NarI NciI NcoI NdeI NdeII NgoMIV NheI NotI NruI NsiI NspI PacI PaeR7I PflFI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI PvuII RcaI RsaI RsrII SacII SalI SapI Sau3AI SbfI ScaI SexAI SfiI SfoI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI Tsp509I Tth111I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 36 enzymes) # ################################### AspHI 50 GAGCTC G[AT]GC[AT]^C AvaII 108 GGACC G^G[AT]CC AvrII 39 CCTAGG C^CTAGG BanI 34 GGTGCC G^G[CT][GA]CC BanII 50 GAGCTC G[GA]GC[CT]^C BlnI 39 CCTAGG C^CTAGG BsgI 136 GTGCAG GTGCAG(16/14) BsiHKAI 50 GAGCTC G[AT]GC[AT]^C BspMI 81 ACCTGC ACCTGC(4/8) BstBI 62 TTCGAA TT^CGAA BstF5I 351 GGATG GGATG(2/0) BstXI 162 CCACCATTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG DraII 314 GGGGCCT [GA]G^G[ACGT]CC[CT] DraIII 433 CACGATGTG CAC[ACGT][ACGT][ACGT]^GTG EarI 299 CTCTTC CTCTTC(1/4) EcoO109I 314 GGGGCCT [GA]G^G[ACGT]CC[CT] FokI 351 GGATG GGATG(9/13) HapII 241 CCGG C^CGG HinfI 255 GAATC G^A[ACGT]TC HpaII 241 CCGG C^CGG HphI 154 TCACC GGTGA(8/7) Ksp632I 299 CTCTTC CTCTTC(1/4) MseI 400 TTAA T^TAA MslI 346 CATGGGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspI 241 CCGG C^CGG NspV 62 TTCGAA TT^CGAA PflMI 461 CCATGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PstI 287 CTGCAG CTGCA^G SacI 50 GAGCTC GAGCT^C SfcI 287 CTGCAG C^T[GA][CT]AG SfuI 62 TTCGAA TT^CGAA TaqI 63 TCGA T^CGA TfiI 255 GAATC G^A[AT]TC Tru9I 400 TTAA T^TAA TthHB8I 63 TCGA T^CGA Van91I 461 CCATGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 17 enzymes) # ################################### AciI 4 GCGG CCGC(-3/-1) 32 GCGG BfaI 40 CTAG C^TAG 200 CTAG BmyI 35 GTGCCC G[AGT]GC[ACT]^C 50 GAGCTC BpmI 68 CTCCAG CTGGAG(16/14) 12 CTGGAG Bsp1286I 35 GTGCCC G[AGT]GC[ACT]^C 50 GAGCTC BssKI 228 CCTGG ^CC[ACGT]GG 282 CCTGG BstNI 228 CCTGG CC^[AT]GG 282 CCTGG Cfr13I 108 GGACC G^G[ACGT]CC 315 GGGCC DdeI 19 CTGAG C^T[ACGT]AG 93 CTCAG EcoRII 228 CCTGG ^CC[AT]GG 282 CCTGG HaeIII 46 GGCC GG^CC 316 GGCC MaeI 40 CTAG C^TAG 200 CTAG MboII 102 TCTTC GAAGA(8/7) 300 TCTTC MvaI 228 CCTGG CC^[AT]GG 282 CCTGG Sau96I 108 GGACC G^G[ACGT]CC 315 GGGCC ScrFI 228 CCTGG CC^[ACGT]GG 282 CCTGG Tsp45I 153 GTCAC ^GT[GC]AC 308 GTCAC ######################################### # 3 OR MORE CUT ENZYMES ( 18 enzymes) # ######################################### AluI BbvI BsaJI BsiYI BslI BsrI EcoT14I Fnu4HI ItaI MaeIII MnlI MwoI NlaIII NlaIV SfaNI StyI TseI TspRI