(Linear) (Six-base) MAPSORT of: KIEE5109.seq Check: 7112 from: 1 to: 477 KIEE5109.orf With 182 enzymes: SgfI * January 29, 2014 11:46 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 88 477 Size: 88 389 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 383 477 Size: 383 94 AvrII C'CTAG_G Cuts at: 0 39 477 Size: 39 438 BanI G'GyrC_C Cuts at: 0 34 477 Size: 34 443 BanII G_rGCy'C Cuts at: 0 54 477 Size: 54 423 Bme1580I G_kGCm'C Cuts at: 0 39 477 Size: 39 438 BmrI ACTGGGnnnn_n' Cuts at: 0 321 477 Size: 321 156 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 33 53 477 Size: 33 20 424 Fragments arranged by size: 424 33 20 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 6 36 477 Size: 6 30 441 Fragments arranged by size: 441 30 6 BseYI C'CCAG_C Cuts at: 0 409 477 Size: 409 68 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 157 477 Size: 157 320 BsiHKAI G_wGCw'C Cuts at: 0 54 477 Size: 54 423 Bsp1286I G_dGCh'C Cuts at: 0 39 54 477 Size: 39 15 423 Fragments arranged by size: 423 39 15 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 90 477 Size: 90 387 BstBI TT'CG_AA Cuts at: 0 63 477 Size: 63 414 BstXI CCAn_nnnn'nTGG Cuts at: 0 169 477 Size: 169 308 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 283 477 Size: 283 194 DraIII CAC_nnn'GTG Cuts at: 0 438 477 Size: 438 39 EarI CTCTTCn'nnn_ Cuts at: 0 305 477 Size: 305 172 EcoICRI GAG'CTC Cuts at: 0 52 477 Size: 52 425 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 33 53 88 477 Size: 33 20 35 389 Fragments arranged by size: 389 35 33 20 EcoO109I rG'GnC_Cy Cuts at: 0 315 477 Size: 315 162 MslI CAynn'nnrTG Cuts at: 0 350 477 Size: 350 127 PflMI CCAn_nnn'nTGG Cuts at: 0 467 477 Size: 467 10 PstI C_TGCA'G Cuts at: 0 291 477 Size: 291 186 SacI G_AGCT'C Cuts at: 0 54 477 Size: 54 423 SfcI C'TryA_G Cuts at: 0 287 477 Size: 287 190 StyI C'CwwG_G Cuts at: 0 39 176 318 371 477 Size: 39 137 142 53 106 Fragments arranged by size: 142 137 106 53 39 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 125 477 Size: 125 352 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 6 38 477 Size: 6 32 439 Fragments arranged by size: 439 32 6 Enzymes that do cut: AcuI AlfI AvrII BanI BanII Bme1580I BmrI BpmI BsaXI BseYI BsgI BsiHKAI Bsp1286I BspMI BstBI BstXI BtgZI DraIII EarI EcoICRI Eco57MI EcoO109I MslI PflMI PstI SacI SfcI StyI TaqII TstI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AfeI AflII AflIII AgeI AhdI AleI AloI AlwNI ApaI ApaLI ApoI AscI AseI AsiSI AvaI BaeI BamHI BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI BmgBI BmtI BplI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI BsiEI BsiWI BsmI BsmBI BspEI BspHI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstEII BstYI BstZ17I Bsu36I BtgI BtsI ClaI CspCI DraI DrdI EaeI EagI EciI EcoNI EcoRI EcoRV FalI FseI FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI PacI PasI PciI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PvuI PvuII RsrII SacII SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI SwaI TatI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI