Restriction map for
pFN21AE1557
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# NO CUT ENZYMES ( 146 enzymes) #
##################################
AatII AccI AccII AccIII AclI
AcsI AflII AhdI Alw44I Aor51HI
ApaI ApaLI ApoI AscI AseI
AsnI Asp700I AspEI AspHI AspI
AviII AvrII BalI BanII BbrPI
BbsI BbvCI BcgI BciVI BcnI
BfrI BglI BglII BlnI BlpI
BmyI Bpu1102I BpuAI BsaBI BseAI
BsiEI BsiHKAI BsiWI BsmI Bsp1286I
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrBI BsrDI BsrGI BssHII
Bst1107I BstBI BstEII BstPI BstUI
BstXI BstZ17I CelII ClaI CpoI
DraI DraIII DrdI DsaI EagI
Eam1105I EarI EclXI Eco47III Eco52I
EcoNI EcoO65I EcoRI EcoRV EcoT14I
FauI FseI FspI HincII HindII
HindIII HpaI Ksp632I KspI MamI
MluI MluNI MroI MscI MspA1I
MvnI NciI NcoI NdeI NheI
NotI NruI NspV PacI PaeR7I
PflFI PflMI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PstI PvuI
PvuII RcaI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfcI SfiI SfuI SmaI SnaBI
SpeI Sse8387I SspBI StuI StyI
SwaI TaqI TfiI Tth111I TthHB8I
Van91I XbaI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 44 enzymes) #
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Acc65I 821 GGTACC G^GTACC
AcyI 169 GGCGCC G[GA]^CG[CT]C
AflIII 107 ACACGT A^C[GA][CT]GT
AgeI 4 ACCGGT A^CCGGT
AocI 634 CCTGAGG CC^T[ACGT]AGG
Asp718I 821 GGTACC G^GTACC
AvaI 37 CCCGAG C^[CT]CG[GA]G
BbeI 169 GGCGCC GGCGC^C
BclI 258 TGATCA T^GATCA
BfaI 223 CTAG C^TAG
BsaAI 505 TACGTG [CT]AC^GT[GA]
BsaHI 169 GGCGCC G[GA]^CG[CT]C
BsaI 440 GAGACC GGTCTC(1/5)
BsaWI 4 ACCGGT [AT]^CCGG[AT]
BsgI 508 GTGCAG GTGCAG(16/14)
BsmBI 118 CGTCTC CGTCTC(1/5)
BsoBI 37 CCCGAG C^[CT]CG[GA]G
BssSI 828 CACGAG CACGAG(-5/-1)
BstAPI 838 GCATCACTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 634 CCTGAGG CC^T[ACGT]AGG
EaeI 134 CGGCCA [CT]^GGCC[GA]
Eco81I 634 CCTGAGG CC^T[ACGT]AGG
EcoT22I 241 ATGCAT ATGCA^T
FbaI 258 TGATCA T^GATCA
HaeII 169 GGCGCC [GA]GCGC^[CT]
Hin1I 169 GGCGCC G[GA]^CG[CT]C
HphI 973 TCACC GGTGA(8/7)
KasI 169 GGCGCC G^GCGCC
KpnI 821 GGTACC GGTAC^C
MaeI 223 CTAG C^TAG
MfeI 232 CAATTG C^AATTG
MslI 108 CACGTCCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI 232 CAATTG C^AATTG
NaeI 132 GCCGGC GCC^GGC
NarI 169 GGCGCC GG^CGCC
NgoMIV 132 GCCGGC G^CCGGC
NsiI 241 ATGCAT ATGCA^T
NspI 204 GCATGC [GA]CATG^[CT]
PinAI 4 ACCGGT A^CCGGT
SfoI 169 GGCGCC GGC^GCC
SgrAI 165 CACCGGCG C[GA]^CCGG[CT]G
SmlI 151 CTCAAG C^T[CT][GA]AG
SphI 204 GCATGC GCATG^C
XcmI 199 CCAACGCATGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AciI 468 CCGC CCGC(-3/-1)
147 GCGG
AluI 23 AGCT AG^CT
589 AGCT
AlwNI 82 CAGTTCCTG CAG[ACGT][ACGT][ACGT]^CTG
431 CAGTGTCTG
BamHI 369 GGATCC G^GATCC
783 GGATCC
BanI 169 GGCGCC G^G[CT][GA]CC
821 GGTACC
BpmI 210 CTGGAG CTGGAG(16/14)
437 CTGGAG
BsaJI 38 CCGAGG C^C[ACGT][ACGT]GG
101 CCTGGG
BseRI 51 CTCCTC GAGGAG(10/8)
96 CTCCTC
BsiYI 37 CCCGAGGATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
919 CCTGAAGATGG
BslI 37 CCCGAGGATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
919 CCTGAAGATGG
BstYI 369 GGATCC [GA]^GATC[CT]
783 GGATCC
DraII 277 GGGACCC [GA]G^G[ACGT]CC[CT]
394 GGGACCT
EcoO109I 277 GGGACCC [GA]G^G[ACGT]CC[CT]
394 GGGACCT
HgaI 117 GCGTC GACGC(5/10)
880 GCGTC
HinfI 523 GAGTC G^A[ACGT]TC
543 GACTC
MaeII 109 ACGT A^CGT
506 ACGT
MflI 369 GGATCC [GA]^GATC[CT]
783 GGATCC
MwoI 24 GCTGAACCAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
838 GCATCACTTGC
PleI 543 GACTC GAGTC(4/5)
523 GAGTC
PpuMI 277 GGGACCC [GA]G^G[AT]CC[CT]
394 GGGACCT
SspI 688 AATATT AAT^ATT
775 AATATT
Tsp509I 233 AATT ^AATT
812 AATT
XhoII 369 GGATCC [GA]^GATC[CT]
783 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 45 enzymes) #
#########################################
AfaI AlwI AvaII BbvI BsmAI
BsmFI BsrFI BsrI BssKI BstF5I
BstNI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII EcoRII Fnu4HI FokI
HaeIII HapII HhaI HinP1I HpaII
ItaI MaeIII MboI MboII MnlI
MseI MspI MvaI NdeII NlaIII
NlaIV RsaI Sau3AI Sau96I ScrFI
SfaNI Tru9I TseI Tsp45I TspRI