(Circular) (Six-base) MAPSORT of: pFN21AE1557.seq Check: 9363 from: 1 to: 5689 pFN21AE1557 5689 bp With 182 enzymes: SgfI * August 16, 2010 14:47 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3499 278 Size: 53 83 186 936 1963 2468 Fragments arranged by size: 2468 1963 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3032 1937 Size: 1095 4594 Acc65I G'GTAC_C Cuts at: 2826 3010 2826 Size: 184 5505 AclI AA'CG_TT Cuts at: 1563 3816 4189 1563 Size: 2253 373 3063 Fragments arranged by size: 3063 2253 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2839 2946 3752 5136 5404 1632 Size: 1207 107 806 1384 268 1917 Fragments arranged by size: 1917 1384 1207 806 268 107 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5492 AflIII A'CryG_T Cuts at: 1203 2112 4588 5565 1203 Size: 909 2476 977 1327 Fragments arranged by size: 2476 1327 977 909 AgeI A'CCGG_T Cuts at: 2009 4520 2009 Size: 2511 3178 AhdI GACnn_n'nnGTC Cuts at: 4418 4418 Size: 5689 AleI CACnn'nnGTG Cuts at: 1166 2117 1166 Size: 951 4738 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5689 AlwNI CAG_nnn'CTG Cuts at: 2092 2441 3432 5004 2092 Size: 349 991 1572 2777 Fragments arranged by size: 2777 1572 991 349 ApaLI G'TGCA_C Cuts at: 3746 4902 3746 Size: 1156 4533 ApoI r'AATT_y Cuts at: 1493 2998 3224 3376 5485 1493 Size: 1505 226 152 2109 1697 Fragments arranged by size: 2109 1697 1505 226 152 AseI AT'TA_AT Cuts at: 160 1032 4243 160 Size: 872 3211 1606 Fragments arranged by size: 3211 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5689 AvaI C'yCGr_G Cuts at: 1870 1943 2042 3014 1870 Size: 73 99 972 4545 Fragments arranged by size: 4545 972 99 73 BamHI G'GATC_C Cuts at: 2374 2788 3019 3386 2374 Size: 414 231 367 4677 Fragments arranged by size: 4677 414 367 231 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2174 2826 3010 4465 618 Size: 325 217 627 387 652 184 1455 1842 Fragments arranged by size: 1842 1455 652 627 387 325 217 184 BanII G_rGCy'C Cuts at: 1391 3008 1391 Size: 1617 4072 BbeI G_GCGC'C Cuts at: 2178 2178 Size: 5689 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4718 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3880 3914 1903 Size: 34 1943 34 3678 Fragments arranged by size: 3678 1943 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3581 4791 3581 Size: 1210 4479 BclI T'GATC_A Cuts at: 1548 2263 1548 Size: 715 4974 BfrBI ATG'CAT Cuts at: 2248 2248 Size: 5689 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4300 136 Size: 107 122 71 631 605 2628 1525 Fragments arranged by size: 2628 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5684 5684 Size: 5689 BlpI GC'TnA_GC Cuts at: 3106 3106 Size: 5689 Bme1580I G_kGCm'C Cuts at: 1790 3750 4906 1790 Size: 1960 1156 2573 Fragments arranged by size: 2573 1960 1156 BmgBI CAC'GTC Cuts at: 1318 2115 1318 Size: 797 4892 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2080 2705 3420 Size: 416 53 83 399 24 663 625 715 Cuts at: 3420 4378 442 Size: 958 1753 Fragments arranged by size: 1753 958 715 663 625 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5689 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2236 2463 4349 1239 Size: 997 227 1886 2579 Fragments arranged by size: 2579 1886 997 227 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2141 3814 4679 4977 2141 Size: 1673 865 298 2853 Fragments arranged by size: 2853 1673 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2439 4352 882 Size: 617 940 1913 2219 Fragments arranged by size: 2219 1913 940 617 BsaAI yAC'GTr Cuts at: 493 1204 2512 3480 493 Size: 711 1308 968 2702 Fragments arranged by size: 2702 1308 968 711 BsaBI GATnn'nnATC Cuts at: 5683 5683 Size: 5689 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2175 3496 3878 4513 Size: 53 83 186 936 642 1321 382 635 Cuts at: 4513 275 Size: 1451 Fragments arranged by size: 1451 1321 936 642 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2009 4122 4520 4794 4941 5352 2009 Size: 2113 398 274 147 411 2346 Fragments arranged by size: 2346 2113 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5464 Fragments arranged by size: 5464 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2047 2092 1186 Size: 861 45 4783 Fragments arranged by size: 4783 861 45 BseYI C'CCAG_C Cuts at: 2396 4892 5388 2396 Size: 2496 496 2697 Fragments arranged by size: 2697 2496 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2534 1163 Size: 573 798 4318 Fragments arranged by size: 4318 798 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3139 3900 4928 1540 Size: 275 186 1138 761 1028 2301 Fragments arranged by size: 2301 1138 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3750 3835 4906 3750 Size: 85 1071 4533 Fragments arranged by size: 4533 1071 85 BsmI GAATG_Cn' Cuts at: 3200 3293 3200 Size: 93 5596 BsmBI CGTCTCn'nnnn_ Cuts at: 2129 2129 Size: 5689 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3008 3750 3835 4906 1391 Size: 399 1218 742 85 1071 2174 Fragments arranged by size: 2174 1218 1071 742 399 85 BspEI T'CCGG_A Cuts at: 5352 5352 Size: 5689 BspHI T'CATG_A Cuts at: 3578 5308 3578 Size: 1730 3959 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3044 844 Size: 2200 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3576 1394 Size: 429 1753 3507 Fragments arranged by size: 3507 1753 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4184 4358 65 Size: 1183 2936 174 1396 Fragments arranged by size: 2936 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2009 2137 2171 2448 4333 4520 1700 Size: 110 199 128 34 277 1885 187 2869 Fragments arranged by size: 2869 1885 277 199 187 128 110 34 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5689 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5689 BssSI C'ACGA_G Cuts at: 2833 3749 4761 2833 Size: 916 1012 3761 Fragments arranged by size: 3761 1012 916 BstAPI GCAn_nnn'nTGC Cuts at: 794 2849 794 Size: 2055 3634 BstBI TT'CG_AA Cuts at: 4493 4493 Size: 5689 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5689 BstYI r'GATC_y Cuts at: 1972 2374 2788 3019 3386 3771 3788 5240 5326 Size: 402 414 231 367 385 17 1452 86 Cuts at: 5326 5338 5684 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 414 402 385 367 346 231 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2640 2640 Size: 5689 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5137 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2824 487 Size: 683 56 1598 3352 Fragments arranged by size: 3352 1598 683 56 BtsI GCAGTG_nn' Cuts at: 3205 3999 4019 3205 Size: 794 20 4875 Fragments arranged by size: 4875 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5689 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5654 DraI TTT'AAA Cuts at: 2993 2993 Size: 5689 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4696 809 Size: 507 3380 1802 Fragments arranged by size: 3380 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2139 3136 4025 Size: 54 1425 50 275 18 309 997 889 Cuts at: 4025 5654 8 Size: 1629 43 Fragments arranged by size: 1629 1425 997 889 309 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3136 1537 Size: 275 1324 4090 Fragments arranged by size: 4090 1324 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3619 1353 Size: 2266 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4276 4650 4796 387 Size: 1064 138 2687 374 146 1280 Fragments arranged by size: 2687 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2236 2463 2839 2946 3752 4349 5136 Size: 393 604 227 376 107 806 597 787 Cuts at: 5136 5404 1239 Size: 268 1524 Fragments arranged by size: 1524 806 787 604 597 393 376 268 227 107 EcoO109I rG'GnC_Cy Cuts at: 2283 2400 2283 Size: 117 5572 EcoRI G'AATT_C Cuts at: 2998 3376 2998 Size: 378 5311 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2291 2680 2291 Size: 389 5300 FspI TGC'GCA Cuts at: 4195 4195 Size: 5689 HaeII r_GCGC'y Cuts at: 1678 2178 4836 1678 Size: 500 2658 2531 Fragments arranged by size: 2658 2531 500 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2039 2071 2392 2424 4330 Size: 32 135 32 467 32 321 32 1906 Cuts at: 4330 4362 4404 4436 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1906 467 321 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3033 3279 669 Size: 988 281 1095 246 3079 Fragments arranged by size: 3079 1095 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5689 HpaI GTT'AAC Cuts at: 3279 3279 Size: 5689 KasI G'GCGC_C Cuts at: 2174 2174 Size: 5689 KpnI G_GTAC'C Cuts at: 2830 3014 2830 Size: 184 5505 MfeI C'AATT_G Cuts at: 2237 3288 2237 Size: 1051 4638 MluI A'CGCG_T Cuts at: 5565 5565 Size: 5689 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2228 4803 4987 1305 Size: 923 2575 184 2007 Fragments arranged by size: 2575 2007 923 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3867 Fragments arranged by size: 3867 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2117 3647 4006 4165 518 Size: 648 951 1530 359 159 2042 Fragments arranged by size: 2042 1530 951 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3105 3782 4930 5175 1606 Size: 1499 677 1148 245 2120 Fragments arranged by size: 2120 1499 1148 677 245 NaeI GCC'GGC Cuts at: 2139 2139 Size: 5689 NarI GG'CG_CC Cuts at: 2175 2175 Size: 5689 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5137 NdeI CA'TA_TG Cuts at: 387 387 Size: 5689 NgoMIV G'CCGG_C Cuts at: 2137 2137 Size: 5689 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5689 NotI GC'GGCC_GC Cuts at: 3136 3136 Size: 5689 NsiI A_TGCA'T Cuts at: 2250 2250 Size: 5689 NspI r_CATG'y Cuts at: 1132 1229 2213 3047 4592 1132 Size: 97 984 834 1545 2229 Fragments arranged by size: 2229 1545 984 834 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5689 PciI A'CATG_T Cuts at: 4588 4588 Size: 5689 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5689 PfoI T'CCnGG_A Cuts at: 2181 2181 Size: 5689 PmeI GTTT'AAAC Cuts at: 2993 2993 Size: 5689 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3758 3790 5271 5303 3758 Size: 32 1481 32 4144 Fragments arranged by size: 4144 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2283 2400 2283 Size: 117 5572 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5689 PsiI TTA'TAA Cuts at: 3259 3259 Size: 5689 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2492 2524 2492 Size: 32 5657 PstI C_TGCA'G Cuts at: 830 3041 830 Size: 2211 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5689 SalI G'TCGA_C Cuts at: 1936 3031 1936 Size: 1095 4594 SanDI GG'GwC_CC Cuts at: 2283 2283 Size: 5689 SbfI CC_TGCA'GG Cuts at: 3041 3041 Size: 5689 ScaI AGT'ACT Cuts at: 3937 5649 3937 Size: 1712 3977 SfcI C'TryA_G Cuts at: 826 1045 3037 4172 4853 5044 826 Size: 219 1992 1135 681 191 1471 Fragments arranged by size: 1992 1471 1135 681 219 191 SfoI GGC'GCC Cuts at: 2176 2176 Size: 5689 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5689 SgrAI Cr'CCGG_yG Cuts at: 1700 2171 1700 Size: 471 5218 SmaI CCC'GGG Cuts at: 1872 3016 1872 Size: 1144 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2156 3793 4694 4956 820 Size: 197 926 213 1637 901 262 1553 Fragments arranged by size: 1637 1553 926 901 262 213 197 SnaBI TAC'GTA Cuts at: 493 3480 493 Size: 2987 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5689 SphI G_CATG'C Cuts at: 1132 2213 3047 1132 Size: 1081 834 3774 Fragments arranged by size: 3774 1081 834 SspI AAT'ATT Cuts at: 5 52 2695 2782 3613 5 Size: 47 2643 87 831 2081 Fragments arranged by size: 2643 2081 831 87 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5689 StyI C'CwwG_G Cuts at: 513 1065 3128 513 Size: 552 2063 3074 Fragments arranged by size: 3074 2063 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3716 3733 3886 4071 4516 325 Size: 1255 2136 17 153 185 445 1498 Fragments arranged by size: 2136 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2984 3935 5647 Size: 274 80 33 51 1443 1007 951 1712 Cuts at: 5647 96 Size: 138 Fragments arranged by size: 1712 1443 1007 951 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2905 2937 5649 5681 2905 Size: 32 2712 32 2913 Fragments arranged by size: 2913 2712 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5689 XbaI T'CTAG_A Cuts at: 3025 3025 Size: 5689 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2211 2211 Size: 5689 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5689 XmaI C'CCGG_G Cuts at: 1870 3014 1870 Size: 1144 4545 XmnI GAAnn'nnTTC Cuts at: 3818 3818 Size: 5689 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3497 276 Size: 53 83 186 936 1963 2468 Fragments arranged by size: 2468 1963 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PsrI PstI PvuI SalI SanDI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI BplI Bpu10I BsiWI BstXI BstZ17I DraIII EcoICRI EcoNI EcoRV FseI FspAI NruI PacI PmlI PspOMI PspXI PvuII RsrII SacI SacII SapI SexAI SfiI SrfI SwaI