################################## # NO CUT ENZYMES ( 93 enzymes) # ################################## AatII Acc65I AccIII AclI AcsI AflII AgeI ApoI AscI AseI AsnI Asp700I Asp718I AvrII BbrPI BbsI BciVI BclI BfaI BfrI BlnI BpuAI BsaAI BsaBI BseAI BsiWI BsmI Bsp1407I BspDI BspEI BsrGI Bst1107I BstZ17I ClaI CpoI DraI EagI EclXI Eco52I EcoRI EcoRV EcoT22I FbaI FseI HindIII HpaI KpnI KspI MaeI MamI MfeI MluI MroI MseI MunI NaeI NdeI NgoMIV NheI NotI NruI NsiI PacI PaeR7I PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RsrII SacII SapI SbfI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI TfiI Tru9I Tsp509I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 47 enzymes) # ################################### AccI 2452 GTCGAC GT^[AC][GT]AC AflIII 1658 ACATGT A^C[GA][CT]GT AocI 450 CCTTAGG CC^T[ACGT]AGG Aor51HI 342 AGCGCT AGC^GCT AvaI 2359 CCCGAG C^[CT]CG[GA]G AviII 1392 TGCGCA TGC^GCA BbeI 2649 GGCGCC GGCGC^C BglII 1490 AGATCT A^GATCT BlpI 2058 GCTGAGC GC^T[ACGT]AGC Bpu1102I 2058 GCTGAGC GC^T[ACGT]AGC BsaWI 836 ACCGGA [AT]^CCGG[AT] BsiEI 2409 CGACCG CG[GA][CT]^CG BsoBI 2359 CCCGAG C^[CT]CG[GA]G BspHI 2441 TCATGA T^CATGA BspLU11I 1658 ACATGT A^CATGT BssHII 1605 GCGCGC G^CGCGC BstAPI 1881 GCAGCTGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstBI 682 TTCGAA TT^CGAA BstEII 284 GGTTACC G^GT[ACGT]ACC BstPI 284 GGTTACC G^GT[ACGT]ACC Bsu36I 450 CCTTAGG CC^T[ACGT]AGG CelII 2058 GCTGAGC GC^T[ACGT]AGC DraIII 1476 CACCCGGTG CAC[ACGT][ACGT][ACGT]^GTG Eco47III 342 AGCGCT AGC^GCT Eco81I 450 CCTTAGG CC^T[ACGT]AGG EcoO65I 284 GGTTACC G^GT[ACGT]ACC FspI 1392 TGCGCA TGC^GCA HinfI 1459 GACTC G^A[ACGT]TC KasI 2649 GGCGCC G^GCGCC NarI 2649 GGCGCC GG^CGCC NspI 1658 ACATGT [GA]CATG^[CT] NspV 682 TTCGAA TT^CGAA PflMI 935 CCATCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 1459 GACTC GAGTC(4/5) PstI 1078 CTGCAG CTGCA^G RcaI 2441 TCATGA T^CATGA SacI 1435 GAGCTC GAGCT^C SalI 2452 GTCGAC G^TCGAC ScaI 2630 AGTACT AGT^ACT SexAI 2537 ACCAGGT A^CC[AT]GGT SfcI 1078 CTGCAG C^T[GA][CT]AG SfiI 1755 GGCCATCCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SfoI 2649 GGCGCC GGC^GCC SfuI 682 TTCGAA TT^CGAA SmlI 244 CTCAAG C^T[CT][GA]AG TspRI 1501 CAGTG CA[GC]TG[ACGT][ACGT]^ Van91I 935 CCATCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 19 enzymes) # ################################### Alw44I 316 GTGCAC G^TGCAC 1021 GTGCAC AlwNI 988 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG 1879 CAGCAGCTG ApaLI 316 GTGCAC G^TGCAC 1021 GTGCAC AspI 655 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC 2536 GACCAGGTC BalI 902 TGGCCA TGG^CCA 1763 TGGCCA BamHI 923 GGATCC G^GATCC 1991 GGATCC BcgI 1383 CGACCTGGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 203 GCACACAGATCG BsmBI 236 GAGACG CGTCTC(1/5) 1324 GAGACG BssSI 1273 CACGAG CACGAG(-5/-1) 1366 CACGAG BstXI 713 CCAAGATGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1973 CCAATGTTGTGG DrdI 652 GACGACCCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 774 GACCTATGTGTC EarI 394 GAAGAG CTCTTC(1/4) 481 GAAGAG Ksp632I 394 GAAGAG CTCTTC(1/4) 481 GAAGAG MaeII 545 ACGT A^CGT 2399 ACGT MluNI 902 TGGCCA TGG^CCA 1763 TGGCCA MscI 902 TGGCCA TGG^CCA 1763 TGGCCA NcoI 1640 CCATGG C^CATGG 2183 CCATGG PflFI 655 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC 2536 GACCAGGTC Tth111I 655 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC 2536 GACCAGGTC ######################################### # 3 OR MORE CUT ENZYMES ( 99 enzymes) # ######################################### AccII AciI AcyI AfaI AhdI AluI AlwI ApaI AspEI AspHI AvaII BanI BanII BbvCI BbvI BcnI BglI BmyI BpmI BsaHI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BspMI BsrBI BsrDI BsrFI BsrI BssKI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI Eam1105I EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HincII HindII HpaII HphI ItaI MaeIII MboI MboII MflI MnlI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV PpuMI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI StuI StyI TaqI TseI Tsp45I TthHB8I XcmI XhoII