(Linear) (Six-base) MAPSORT of: KIEE1354.seq Check: 7427 from: 1 to: 2733 KIEE1354.orf With 182 enzymes: SgfI * July 15, 2010 18:37 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 197 1955 2733 Size: 197 1758 778 Fragments arranged by size: 1758 778 197 AccI GT'mk_AC Cuts at: 0 2453 2733 Size: 2453 280 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1327 2089 2733 Size: 1327 762 644 Fragments arranged by size: 1327 762 644 AfeI AGC'GCT Cuts at: 0 344 2733 Size: 344 2389 AflIII A'CryG_T Cuts at: 0 1658 2733 Size: 1658 1075 AhdI GACnn_n'nnGTC Cuts at: 0 1529 2502 2694 2733 Size: 1529 973 192 39 Fragments arranged by size: 1529 973 192 39 AlwNI CAG_nnn'CTG Cuts at: 0 993 1884 2733 Size: 993 891 849 Fragments arranged by size: 993 891 849 ApaI G_GGCC'C Cuts at: 0 1988 2360 2665 2733 Size: 1988 372 305 68 Fragments arranged by size: 1988 372 305 68 ApaLI G'TGCA_C Cuts at: 0 316 1021 2733 Size: 316 705 1712 Fragments arranged by size: 1712 705 316 AvaI C'yCGr_G Cuts at: 0 2359 2733 Size: 2359 374 BamHI G'GATC_C Cuts at: 0 923 1991 2733 Size: 923 1068 742 Fragments arranged by size: 1068 923 742 BanI G'GyrC_C Cuts at: 0 515 1481 1887 2649 2680 2733 Size: 515 966 406 762 31 53 Fragments arranged by size: 966 762 515 406 53 31 BanII G_rGCy'C Cuts at: 0 245 506 1439 1988 2360 2665 2733 Size: 245 261 933 549 372 305 68 Fragments arranged by size: 933 549 372 305 261 245 68 BbeI G_GCGC'C Cuts at: 0 2653 2733 Size: 2653 80 BbvCI CC'TCA_GC Cuts at: 0 643 1096 2591 2733 Size: 643 453 1495 142 Fragments arranged by size: 1495 643 453 142 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 192 226 1372 1406 2733 Size: 192 34 1146 34 1327 Fragments arranged by size: 1327 1146 192 34 34 BglI GCCn_nnn'nGGC Cuts at: 0 1762 1969 2647 2733 Size: 1762 207 678 86 Fragments arranged by size: 1762 678 207 86 BglII A'GATC_T Cuts at: 0 1490 2733 Size: 1490 1243 BlpI GC'TnA_GC Cuts at: 0 2059 2733 Size: 2059 674 Bme1580I G_kGCm'C Cuts at: 0 320 962 1025 1988 2360 2665 2678 2685 Size: 320 642 63 963 372 305 13 7 Cuts at: 2685 2733 Size: 48 Fragments arranged by size: 963 642 372 320 305 63 48 13 7 BmgBI CAC'GTC Cuts at: 0 2400 2733 Size: 2400 333 BmrI ACTGGGnnnn_n' Cuts at: 0 120 2733 Size: 120 2613 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 259 2733 Size: 259 2474 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 283 937 967 1015 1078 1243 1567 1588 Size: 283 654 30 48 63 165 324 21 Cuts at: 1588 1636 1933 2068 2140 2733 Size: 48 297 135 72 593 Fragments arranged by size: 654 593 324 297 283 165 135 72 63 48 48 30 21 Bpu10I CC'TnA_GC Cuts at: 0 290 643 1096 2591 2733 Size: 290 353 453 1495 142 Fragments arranged by size: 1495 453 353 290 142 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 229 2733 Size: 229 2504 BsaI GGTCTCn'nnnn_ Cuts at: 0 384 478 1143 2506 2733 Size: 384 94 665 1363 227 Fragments arranged by size: 1363 665 384 227 94 BsaHI Gr'CG_yC Cuts at: 0 368 1508 1736 2273 2314 2650 2669 2733 Size: 368 1140 228 537 41 336 19 64 Fragments arranged by size: 1140 537 368 336 228 64 41 19 BsaWI w'CCGG_w Cuts at: 0 836 2733 Size: 836 1897 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 1444 1474 2733 Size: 1444 30 1259 Fragments arranged by size: 1444 1259 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 1207 1696 1930 2023 2377 2608 2733 Size: 1207 489 234 93 354 231 125 Fragments arranged by size: 1207 489 354 234 231 125 93 BseYI C'CCAG_C Cuts at: 0 43 2204 2352 2733 Size: 43 2161 148 381 Fragments arranged by size: 2161 381 148 43 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 574 1060 1570 1897 2733 Size: 574 486 510 327 836 Fragments arranged by size: 836 574 510 486 327 BsiEI CG_ry'CG Cuts at: 0 2412 2733 Size: 2412 321 BsiHKAI G_wGCw'C Cuts at: 0 320 878 1025 1439 1946 2192 2733 Size: 320 558 147 414 507 246 541 Fragments arranged by size: 558 541 507 414 320 246 147 BsmBI CGTCTCn'nnnn_ Cuts at: 0 230 1318 2733 Size: 230 1088 1415 Fragments arranged by size: 1415 1088 230 Bsp1286I G_dGCh'C Cuts at: 0 245 320 506 878 962 1025 1439 1946 Size: 245 75 186 372 84 63 414 507 Cuts at: 1946 1988 2192 2360 2665 2678 2685 2733 Size: 42 204 168 305 13 7 48 Fragments arranged by size: 507 414 372 305 245 204 186 168 84 75 63 48 42 13 7 BspHI T'CATG_A Cuts at: 0 2441 2733 Size: 2441 292 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 131 197 1955 2123 2733 Size: 131 66 1758 168 610 Fragments arranged by size: 1758 610 168 131 66 BsrBI CCG'CTC Cuts at: 0 282 300 1227 2733 Size: 282 18 927 1506 Fragments arranged by size: 1506 927 282 18 BsrDI GCAATG_nn' Cuts at: 0 63 1424 2225 2733 Size: 63 1361 801 508 Fragments arranged by size: 1361 801 508 63 BsrFI r'CCGG_y Cuts at: 0 608 1013 1103 2411 2733 Size: 608 405 90 1308 322 Fragments arranged by size: 1308 608 405 322 90 BssHII G'CGCG_C Cuts at: 0 1605 2733 Size: 1605 1128 BssSI C'ACGA_G Cuts at: 0 1273 1366 2733 Size: 1273 93 1367 Fragments arranged by size: 1367 1273 93 BstAPI GCAn_nnn'nTGC Cuts at: 0 1887 2733 Size: 1887 846 BstBI TT'CG_AA Cuts at: 0 683 2733 Size: 683 2050 BstEII G'GTnAC_C Cuts at: 0 284 2733 Size: 284 2449 BstXI CCAn_nnnn'nTGG Cuts at: 0 720 1980 2733 Size: 720 1260 753 Fragments arranged by size: 1260 753 720 BstYI r'GATC_y Cuts at: 0 839 923 1211 1490 1991 2258 2733 Size: 839 84 288 279 501 267 475 Fragments arranged by size: 839 501 475 288 279 267 84 Bsu36I CC'TnA_GG Cuts at: 0 451 2733 Size: 451 2282 BtgI C'CryG_G Cuts at: 0 476 1640 2183 2705 2733 Size: 476 1164 543 522 28 Fragments arranged by size: 1164 543 522 476 28 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 2529 2622 2733 Size: 2529 93 111 Fragments arranged by size: 2529 111 93 DraIII CAC_nnn'GTG Cuts at: 0 1481 2733 Size: 1481 1252 DrdI GACnn_nn'nnGTC Cuts at: 0 658 780 2733 Size: 658 122 1953 Fragments arranged by size: 1953 658 122 EaeI y'GGCC_r Cuts at: 0 491 902 1328 1763 2733 Size: 491 411 426 435 970 Fragments arranged by size: 970 491 435 426 411 EarI CTCTTCn'nnn_ Cuts at: 0 389 476 2733 Size: 389 87 2257 Fragments arranged by size: 2257 389 87 EcoICRI GAG'CTC Cuts at: 0 1437 2733 Size: 1437 1296 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 283 937 967 1015 1078 1243 1327 1567 Size: 283 654 30 48 63 165 84 240 Cuts at: 1567 1588 1636 1933 2068 2089 2140 2733 Size: 21 48 297 135 21 51 593 Fragments arranged by size: 654 593 297 283 240 165 135 84 63 51 48 48 30 21 21 EcoNI CCTnn'n_nnAGG Cuts at: 0 193 883 1912 2733 Size: 193 690 1029 821 Fragments arranged by size: 1029 821 690 193 EcoO109I rG'GnC_Cy Cuts at: 0 120 1647 1692 1984 1985 2042 2148 2356 Size: 120 1527 45 292 1 57 106 208 Cuts at: 2356 2661 2662 2733 Size: 305 1 71 Fragments arranged by size: 1527 305 292 208 120 106 71 57 45 1 1 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 523 2733 Size: 523 2210 FspI TGC'GCA Cuts at: 0 1394 2733 Size: 1394 1339 HaeII r_GCGC'y Cuts at: 0 65 346 1640 2353 2653 2733 Size: 65 281 1294 713 300 80 Fragments arranged by size: 1294 713 300 281 80 65 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 256 288 1515 1547 2488 2520 2680 2712 Size: 256 32 1227 32 941 32 160 32 Cuts at: 2712 2733 Size: 21 Fragments arranged by size: 1227 941 256 160 32 32 32 32 21 HincII GTy'rAC Cuts at: 0 861 1473 2454 2733 Size: 861 612 981 279 Fragments arranged by size: 981 861 612 279 KasI G'GCGC_C Cuts at: 0 2649 2733 Size: 2649 84 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1961 2733 Size: 1961 772 MscI TGG'CCA Cuts at: 0 904 1765 2733 Size: 904 861 968 Fragments arranged by size: 968 904 861 MslI CAynn'nnrTG Cuts at: 0 475 2032 2197 2733 Size: 475 1557 165 536 Fragments arranged by size: 1557 536 475 165 MspA1I CmG'CkG Cuts at: 0 572 1056 1566 1584 1884 2058 2214 2590 Size: 572 484 510 18 300 174 156 376 Cuts at: 2590 2733 Size: 143 Fragments arranged by size: 572 510 484 376 300 174 156 143 18 NarI GG'CG_CC Cuts at: 0 2650 2733 Size: 2650 83 NcoI C'CATG_G Cuts at: 0 1640 2183 2733 Size: 1640 543 550 Fragments arranged by size: 1640 550 543 NspI r_CATG'y Cuts at: 0 1662 2733 Size: 1662 1071 PasI CC'CwG_GG Cuts at: 0 426 2733 Size: 426 2307 PciI A'CATG_T Cuts at: 0 1658 2733 Size: 1658 1075 PflMI CCAn_nnn'nTGG Cuts at: 0 941 2733 Size: 941 1792 PfoI T'CCnGG_A Cuts at: 0 260 464 914 974 1652 1910 2141 2733 Size: 260 204 450 60 678 258 231 592 Fragments arranged by size: 678 592 450 260 258 231 204 60 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 2413 2445 2733 Size: 2413 32 288 Fragments arranged by size: 2413 288 32 PpuMI rG'GwC_Cy Cuts at: 0 120 1647 2042 2733 Size: 120 1527 395 691 Fragments arranged by size: 1527 691 395 120 PspOMI G'GGCC_C Cuts at: 0 1984 2356 2661 2733 Size: 1984 372 305 72 Fragments arranged by size: 1984 372 305 72 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 0 13 45 2733 Size: 13 32 2688 Fragments arranged by size: 2688 32 13 PstI C_TGCA'G Cuts at: 0 1082 2733 Size: 1082 1651 PvuII CAG'CTG Cuts at: 0 572 1056 1566 1584 1884 2058 2214 2590 Size: 572 484 510 18 300 174 156 376 Cuts at: 2590 2733 Size: 143 Fragments arranged by size: 572 510 484 376 300 174 156 143 18 SacI G_AGCT'C Cuts at: 0 1439 2733 Size: 1439 1294 SalI G'TCGA_C Cuts at: 0 2452 2733 Size: 2452 281 SanDI GG'GwC_CC Cuts at: 0 2042 2733 Size: 2042 691 ScaI AGT'ACT Cuts at: 0 2632 2733 Size: 2632 101 SexAI A'CCwGG_T Cuts at: 0 2537 2733 Size: 2537 196 SfcI C'TryA_G Cuts at: 0 1078 2733 Size: 1078 1655 SfiI GGCCn_nnn'nGGCC Cuts at: 0 1762 2733 Size: 1762 971 SfoI GGC'GCC Cuts at: 0 2651 2733 Size: 2651 82 SmlI C'TyrA_G Cuts at: 0 244 2733 Size: 244 2489 StuI AGG'CCT Cuts at: 0 1264 1279 1372 2374 2733 Size: 1264 15 93 1002 359 Fragments arranged by size: 1264 1002 359 93 15 StyI C'CwwG_G Cuts at: 0 494 1034 1640 2183 2276 2733 Size: 494 540 606 543 93 457 Fragments arranged by size: 606 543 540 494 457 93 TatI w'GTAC_w Cuts at: 0 2630 2733 Size: 2630 103 Tth111I GACn'n_nGTC Cuts at: 0 658 2539 2733 Size: 658 1881 194 Fragments arranged by size: 1881 658 194 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 912 942 2181 2244 2733 Size: 912 30 1239 63 489 Fragments arranged by size: 1239 912 489 63 30 Enzymes that do cut: AarI AccI AcuI AfeI AflIII AhdI AlwNI ApaI ApaLI AvaI BamHI BanI BanII BbeI BbvCI BcgI BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI DraIII DrdI EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I FalI FspI HaeII Hin4I HincII KasI MmeI MscI MslI MspA1I NarI NcoI NspI PasI PciI PflMI PfoI PpiI PpuMI PspOMI PsrI PstI PvuII SacI SalI SanDI ScaI SexAI SfcI SfiI SfoI SmlI StuI StyI TatI Tth111I XcmI Enzymes that do not cut: AatII Acc65I AclI AflII AgeI AleI AlfI AloI ApoI AscI AseI AsiSI AvrII BaeI BbsI BciVI BclI BfrBI BmtI BsaAI BsaBI BsiWI BsmI BspEI BsrGI BstZ17I BtsI ClaI CspCI DraI EagI EciI EcoRI EcoRV FseI FspAI HindIII HpaI KpnI MfeI MluI NaeI NdeI NgoMIV NheI NotI NruI NsiI PacI PmeI PmlI PshAI PsiI PspXI PvuI RsrII SacII SapI SbfI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI SwaI TaqII TstI XbaI XhoI XmaI XmnI ZraI