################################## # NO CUT ENZYMES ( 126 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AflII AflIII AgeI AhdI Alw44I AocI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BalI BamHI BbrPI BbsI BbvCI BcgI BciVI BclI BfrI BglII BlnI BpuAI BsaAI BsaBI BsaWI BseAI BsiWI BsmBI BsmI BspDI BspEI BspHI BspLU11I BsrDI BssSI Bst1107I BstBI BstEII BstPI BstXI BstZ17I Bsu36I ClaI CpoI DraI DsaI Eam1105I Eco81I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HincII HindII HindIII HpaI KpnI KspI MaeIII MamI MfeI MluI MluNI MroI MscI MseI MslI MunI NcoI NdeI NheI NlaIII NruI NsiI NspI NspV PacI PflFI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SacII SalI SapI SbfI ScaI SexAI SfiI SfuI SnaBI SpeI SphI Sse8387I SspI SwaI Tru9I Tsp45I Tsp509I Tth111I Van91I XbaI XmnI ################################### # ONE CUT ENZYMES ( 47 enzymes) # ################################### AcyI 361 GGCGCC G[GA]^CG[CT]C Aor51HI 398 AGCGCT AGC^GCT ApaI 500 GGGCCC GGGCC^C AspHI 877 GAGCTC G[AT]GC[AT]^C BbeI 361 GGCGCC GGCGC^C BfaI 418 CTAG C^TAG BglI 429 GCCCGACCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsaHI 361 GGCGCC G[GA]^CG[CT]C BsaI 679 GGTCTC GGTCTC(1/5) BsiHKAI 877 GAGCTC G[AT]GC[AT]^C BsmAI 680 GTCTC GTCTC(1/5) Bsp1407I 122 TGTACA T^GTACA BspMI 591 ACCTGC ACCTGC(4/8) BsrGI 122 TGTACA T^GTACA BstAPI 327 GCACTCGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstYI 188 AGATCC [GA]^GATC[CT] DraIII 328 CACTCGGTG CAC[ACGT][ACGT][ACGT]^GTG EarI 25 GAAGAG CTCTTC(1/4) Eco47III 398 AGCGCT AGC^GCT EcoT14I 143 CCTTGG C^C[AT][AT]GG HgaI 376 GACGC GACGC(5/10) Hin1I 361 GGCGCC G[GA]^CG[CT]C KasI 361 GGCGCC G^GCGCC Ksp632I 25 GAAGAG CTCTTC(1/4) MaeI 418 CTAG C^TAG MflI 188 AGATCC [GA]^GATC[CT] NaeI 358 GCCGGC GCC^GGC NarI 361 GGCGCC GG^CGCC NgoMIV 358 GCCGGC G^CCGGC NotI 310 GCGGCCGC GC^GGCCGC PaeR7I 865 CTCGAG C^TCGAG PpuMI 734 GGGACCC [GA]G^G[AT]CC[CT] SacI 877 GAGCTC GAGCT^C SfoI 361 GGCGCC GGC^GCC SgrAI 357 CGCCGGCG C[GA]^CCGG[CT]G SmaI 503 CCCGGG CCC^GGG SmlI 865 CTCGAG C^T[CT][GA]AG SspBI 122 TGTACA T^GTACA StuI 791 AGGCCT AGG^CCT StyI 143 CCTTGG C^C[AT][AT]GG TaqI 866 TCGA T^CGA TthHB8I 866 TCGA T^CGA XcmI 920 CCAATGGCGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoI 865 CTCGAG C^TCGAG XhoII 188 AGATCC [GA]^GATC[CT] XmaCI 503 CCCGGG C^CCGGG XmaI 503 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 24 enzymes) # ################################### AlwI 189 GATCC GGATC(4/5) 916 GATCC BlpI 457 GCTGAGC GC^T[ACGT]AGC 639 GCTGAGC BpmI 346 CTGGAG CTGGAG(16/14) 463 CTGGAG Bpu1102I 457 GCTGAGC GC^T[ACGT]AGC 639 GCTGAGC BsgI 217 GTGCAG GTGCAG(16/14) 817 GTGCAG BsmFI 734 GGGAC GGGAC(10/14) 777 GGGAC BsrBI 153 GAGCGG CCGCTC(-3/-3) 367 GAGCGG BsrFI 358 GCCGGC [GA]^CCGG[CT] 434 ACCGGC BsrI 345 ACTGG ACTGG(1/-1) 453 ACTGG CelII 457 GCTGAGC GC^T[ACGT]AGC 639 GCTGAGC Cfr10I 358 GCCGGC [GA]^CCGG[CT] 434 ACCGGC DdeI 458 CTGAG C^T[ACGT]AG 640 CTGAG DraII 499 GGGGCCC [GA]G^G[ACGT]CC[CT] 734 GGGACCC DrdI 70 GACAAGGTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 514 GACGGGGAGGTC EagI 311 CGGCCG C^GGCCG 634 CGGCCG EclXI 311 CGGCCG C^GGCCG 634 CGGCCG Eco52I 311 CGGCCG C^GGCCG 634 CGGCCG EcoNI 852 CCTGGTGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 888 CCTGGCAGAGG EcoO109I 499 GGGGCCC [GA]G^G[ACGT]CC[CT] 734 GGGACCC HphI 567 GGTGA GGTGA(8/7) 881 TCACC MaeII 170 ACGT A^CGT 661 ACGT PleI 748 GACTC GAGTC(4/5) 58 GAGTC TfiI 227 GATTC G^A[AT]TC 630 GAATC TspRI 119 CAGTG CA[GC]TG[ACGT][ACGT]^ 707 CACTG ######################################### # 3 OR MORE CUT ENZYMES ( 61 enzymes) # ######################################### AccII AciI AfaI AluI AlwNI AvaI AvaII BanI BanII BbvI BcnI BmyI BsaJI BseRI BsiEI BsiYI BslI BsoBI Bsp1286I BssHII BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr13I DpnII EaeI EcoRII FauI Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HinfI HpaII ItaI MboI MboII MnlI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIV PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI TseI