(Circular) (Six-base) MAPSORT of: pFN21AB6945.seq Check: 6878 from: 1 to: 5338 pFN21AB6945 5338 bp With 182 enzymes: SgfI * August 16, 2010 14:54 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2212 2310 2212 Size: 98 5240 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3148 278 Size: 53 83 186 936 1612 2468 Fragments arranged by size: 2468 1612 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2430 2481 2681 1937 Size: 493 51 200 4594 Fragments arranged by size: 4594 493 200 51 Acc65I G'GTAC_C Cuts at: 2659 2659 Size: 5338 AclI AA'CG_TT Cuts at: 1563 3465 3838 1563 Size: 1902 373 3063 Fragments arranged by size: 3063 1902 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2172 3401 4785 5053 1632 Size: 540 1229 1384 268 1917 Fragments arranged by size: 1917 1384 1229 540 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5141 AflIII A'CryG_T Cuts at: 1203 2425 4237 5214 1203 Size: 1222 1812 977 1327 Fragments arranged by size: 1812 1327 1222 977 AgeI A'CCGG_T Cuts at: 4169 4169 Size: 5338 AhdI GACnn_n'nnGTC Cuts at: 4067 4067 Size: 5338 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5338 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5338 AlwNI CAG_nnn'CTG Cuts at: 2302 3081 4653 2302 Size: 779 1572 2987 Fragments arranged by size: 2987 1572 779 ApaLI G'TGCA_C Cuts at: 3395 4551 3395 Size: 1156 4182 ApoI r'AATT_y Cuts at: 1493 2647 2873 3025 5134 1493 Size: 1154 226 152 2109 1697 Fragments arranged by size: 2109 1697 1154 226 152 AseI AT'TA_AT Cuts at: 160 1032 3892 160 Size: 872 2860 1606 Fragments arranged by size: 2860 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5338 AvaI C'yCGr_G Cuts at: 1870 1943 2663 1870 Size: 73 720 4545 Fragments arranged by size: 4545 720 73 AvrII C'CTAG_G Cuts at: 2455 2455 Size: 5338 BamHI G'GATC_C Cuts at: 2668 3035 2668 Size: 367 4971 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2659 4114 618 Size: 325 217 627 872 1455 1842 Fragments arranged by size: 1842 1455 872 627 325 217 BanII G_rGCy'C Cuts at: 1391 2657 1391 Size: 1266 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4367 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3529 3563 1903 Size: 34 1592 34 3678 Fragments arranged by size: 3678 1592 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3230 4440 3230 Size: 1210 4128 BclI T'GATC_A Cuts at: 1548 1548 Size: 5338 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2448 3949 136 Size: 107 122 71 631 605 776 1501 1525 Fragments arranged by size: 1525 1501 776 631 605 122 107 71 BglII A'GATC_T Cuts at: 5333 5333 Size: 5338 BlpI GC'TnA_GC Cuts at: 2755 2755 Size: 5338 Bme1580I G_kGCm'C Cuts at: 1790 3399 4555 1790 Size: 1609 1156 2573 Fragments arranged by size: 2573 1609 1156 BmgBI CAC'GTC Cuts at: 1318 2428 1318 Size: 1110 4228 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3069 4027 442 Size: 416 53 83 399 24 1652 958 1753 Fragments arranged by size: 1753 1652 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5338 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3998 1239 Size: 2759 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3463 4328 4626 3463 Size: 865 298 4175 Fragments arranged by size: 4175 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4001 882 Size: 617 2502 2219 Fragments arranged by size: 2502 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3129 493 Size: 711 1925 2702 Fragments arranged by size: 2702 1925 711 BsaBI GATnn'nnATC Cuts at: 5332 5332 Size: 5338 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3145 3527 4162 275 Size: 53 83 186 936 1612 382 635 1451 Fragments arranged by size: 1612 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3771 4169 4443 4590 5001 3771 Size: 398 274 147 411 4108 Fragments arranged by size: 4108 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5113 Fragments arranged by size: 5113 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2090 2114 1186 Size: 904 24 4410 Fragments arranged by size: 4410 904 24 BseYI C'CCAG_C Cuts at: 2532 4541 5037 2532 Size: 2009 496 2833 Fragments arranged by size: 2833 2009 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4765 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2788 3549 4577 1540 Size: 275 186 787 761 1028 2301 Fragments arranged by size: 2301 1028 787 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3399 3484 4555 3399 Size: 85 1071 4182 Fragments arranged by size: 4182 1071 85 BsmI GAATG_Cn' Cuts at: 2326 2849 2942 2326 Size: 523 93 4722 Fragments arranged by size: 4722 523 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2657 3399 3484 4555 1391 Size: 399 867 742 85 1071 2174 Fragments arranged by size: 2174 1071 867 742 399 85 BspEI T'CCGG_A Cuts at: 5001 5001 Size: 5338 BspHI T'CATG_A Cuts at: 3227 4957 3227 Size: 1730 3608 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2071 2212 2310 2693 844 Size: 1227 141 98 383 3489 Fragments arranged by size: 3489 1227 383 141 98 BsrBI CCG'CTC Cuts at: 1394 1823 3225 1394 Size: 429 1402 3507 Fragments arranged by size: 3507 1402 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2502 2563 3833 4007 65 Size: 1183 1254 61 1270 174 1396 Fragments arranged by size: 1396 1270 1254 1183 174 61 BsrFI r'CCGG_y Cuts at: 1700 1810 3982 4169 1700 Size: 110 2172 187 2869 Fragments arranged by size: 2869 2172 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5338 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5338 BssSI C'ACGA_G Cuts at: 3398 4410 3398 Size: 1012 4326 BstAPI GCAn_nnn'nTGC Cuts at: 794 2302 2321 794 Size: 1508 19 3811 Fragments arranged by size: 3811 1508 19 BstBI TT'CG_AA Cuts at: 4142 4142 Size: 5338 BstEII G'GTnAC_C Cuts at: 1184 2185 2464 1184 Size: 1001 279 4058 Fragments arranged by size: 4058 1001 279 BstYI r'GATC_y Cuts at: 1972 2668 3035 3420 3437 4889 4975 4987 5333 Size: 696 367 385 17 1452 86 12 346 Cuts at: 5333 1972 Size: 1977 Fragments arranged by size: 1977 1452 696 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2214 2310 513 Size: 552 939 210 96 3541 Fragments arranged by size: 3541 939 552 210 96 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4599 Fragments arranged by size: 4599 683 56 BtsI GCAGTG_nn' Cuts at: 2497 2854 3648 3668 2497 Size: 357 794 20 4167 Fragments arranged by size: 4167 794 357 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5338 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5303 DraI TTT'AAA Cuts at: 2642 2642 Size: 5338 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4345 809 Size: 507 3029 1802 Fragments arranged by size: 3029 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2785 3674 5303 Size: 54 1425 50 275 18 955 889 1629 Cuts at: 5303 8 Size: 43 Fragments arranged by size: 1629 1425 955 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2785 1537 Size: 275 973 4090 Fragments arranged by size: 4090 973 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2400 3268 1353 Size: 1047 868 3423 Fragments arranged by size: 3423 1047 868 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3925 4299 4445 387 Size: 1064 138 2336 374 146 1280 Fragments arranged by size: 2336 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2172 3401 3998 4785 5053 1239 Size: 393 540 1229 597 787 268 1524 Fragments arranged by size: 1524 1229 787 597 540 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2459 2459 Size: 5338 EcoO109I rG'GnC_Cy Cuts at: 2027 2027 Size: 5338 EcoRI G'AATT_C Cuts at: 2647 3025 2647 Size: 378 4960 FspI TGC'GCA Cuts at: 3844 3844 Size: 5338 HaeII r_GCGC'y Cuts at: 1678 4485 1678 Size: 2807 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2220 2252 3979 4011 4053 Size: 32 135 32 648 32 1727 32 42 Cuts at: 4053 4085 1373 Size: 32 2626 Fragments arranged by size: 2626 1727 648 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2482 2682 2928 669 Size: 988 281 544 200 246 3079 Fragments arranged by size: 3079 988 544 281 246 200 HindIII A'AGCT_T Cuts at: 748 748 Size: 5338 HpaI GTT'AAC Cuts at: 2928 2928 Size: 5338 KpnI G_GTAC'C Cuts at: 2663 2663 Size: 5338 MfeI C'AATT_G Cuts at: 2374 2937 2374 Size: 563 4775 MluI A'CGCG_T Cuts at: 5214 5214 Size: 5338 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4452 4636 1305 Size: 3147 184 2007 Fragments arranged by size: 3147 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3516 Fragments arranged by size: 3516 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3296 3655 3814 518 Size: 648 2130 359 159 2042 Fragments arranged by size: 2130 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2754 3431 4579 4824 1606 Size: 1148 677 1148 245 2120 Fragments arranged by size: 2120 1148 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2214 513 Size: 552 939 210 3637 Fragments arranged by size: 3637 939 552 210 NdeI CA'TA_TG Cuts at: 387 387 Size: 5338 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5338 NotI GC'GGCC_GC Cuts at: 2785 2785 Size: 5338 NspI r_CATG'y Cuts at: 1132 1229 2696 4241 1132 Size: 97 1467 1545 2229 Fragments arranged by size: 2229 1545 1467 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5338 PciI A'CATG_T Cuts at: 4237 4237 Size: 5338 PflMI CCAn_nnn'nTGG Cuts at: 1108 2614 1108 Size: 1506 3832 PmeI GTTT'AAAC Cuts at: 2642 2642 Size: 5338 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3407 3439 4920 4952 3407 Size: 32 1481 32 3793 Fragments arranged by size: 3793 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2027 2027 Size: 5338 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5338 PsiI TTA'TAA Cuts at: 2908 2908 Size: 5338 PstI C_TGCA'G Cuts at: 830 2307 2690 830 Size: 1477 383 3478 Fragments arranged by size: 3478 1477 383 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5338 SalI G'TCGA_C Cuts at: 1936 2480 2680 1936 Size: 544 200 4594 Fragments arranged by size: 4594 544 200 SapI GCTCTTCn'nnn_ Cuts at: 2400 2400 Size: 5338 SbfI CC_TGCA'GG Cuts at: 2690 2690 Size: 5338 ScaI AGT'ACT Cuts at: 2333 3586 5298 2333 Size: 1253 1712 2373 Fragments arranged by size: 2373 1712 1253 SfcI C'TryA_G Cuts at: 826 1045 2303 2686 3821 4502 4693 826 Size: 219 1258 383 1135 681 191 1471 Fragments arranged by size: 1471 1258 1135 681 383 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5338 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5338 SmaI CCC'GGG Cuts at: 1872 2665 1872 Size: 793 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3442 4343 4605 820 Size: 197 926 1499 901 262 1553 Fragments arranged by size: 1553 1499 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3129 493 Size: 2636 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5338 SphI G_CATG'C Cuts at: 1132 2696 1132 Size: 1564 3774 SspI AAT'ATT Cuts at: 5 52 3262 5 Size: 47 3210 2081 Fragments arranged by size: 3210 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5338 StyI C'CwwG_G Cuts at: 513 1065 2004 2214 2455 2777 513 Size: 552 939 210 241 322 3074 Fragments arranged by size: 3074 939 552 322 241 210 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2524 2579 3365 3382 3535 3720 4165 Size: 1255 944 55 786 17 153 185 445 Cuts at: 4165 325 Size: 1498 Fragments arranged by size: 1498 1255 944 786 445 185 153 55 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2331 3584 5296 Size: 274 80 33 51 1443 354 1253 1712 Cuts at: 5296 96 Size: 138 Fragments arranged by size: 1712 1443 1253 354 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5298 5330 5298 Size: 32 5306 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5338 XbaI T'CTAG_A Cuts at: 2674 2674 Size: 5338 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2221 2221 Size: 5338 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5338 XmaI C'CCGG_G Cuts at: 1870 2663 1870 Size: 793 4545 XmnI GAAnn'nnTTC Cuts at: 3467 3467 Size: 5338 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3146 276 Size: 53 83 186 936 1612 2468 Fragments arranged by size: 2468 1612 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI