Restriction map for
pFN21AE1360
##################################
# NO CUT ENZYMES ( 154 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
AhdI Alw44I AlwNI Aor51HI ApaLI
AscI AseI AsnI Asp718I AspEI
AspI AvaI AviII AvrII BanI
BbeI BbrPI BcgI BciVI BclI
BfaI BfrI BglI BglII BlnI
BsaAI BsaBI BsaHI BsaI BsaWI
BseAI BseRI BsgI BsiEI BsiWI
BsmBI BsoBI Bsp1407I BspDI BspEI
BspHI BspLU11I BspMI BsrDI BsrFI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstZ17I
Cfr10I ClaI CpoI DraI DraIII
DrdI EagI Eam1105I EarI EclXI
Eco47III Eco52I EcoNI EcoO65I EcoRV
FbaI FseI FspI HaeII HgaI
Hin1I HpaI KasI KpnI Ksp632I
KspI MaeI MaeIII MamI MfeI
MluI MroI MspA1I MunI MvnI
NaeI NarI NdeI NgoMIV NheI
NotI NruI NspI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PstI PvuI PvuII RcaI
RsrII SacII SalI SapI SbfI
SexAI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI SwaI TaqI
Tsp45I Tth111I TthHB8I Van91I XbaI
XcmI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
AcsI 88 GAATTC [GA]^AATT[CT]
AocI 624 CCTGAGG CC^T[ACGT]AGG
ApaI 182 GGGCCC GGGCC^C
ApoI 88 GAATTC [GA]^AATT[CT]
Asp700I 66 GAATCTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 601 GAGCTC G[AT]GC[AT]^C
AvaII 621 GGACC G^G[AT]CC
BamHI 371 GGATCC G^GATCC
BbsI 55 GTCTTC GAAGAC(2/6)
BbvCI 82 GCTGAGG CCTCAGC(-5/-2)
BlpI 640 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 640 GCTGAGC GC^T[ACGT]AGC
BpuAI 55 GTCTTC GAAGAC(2/6)
BsiHKAI 601 GAGCTC G[AT]GC[AT]^C
BsmAI 541 GAGAC GTCTC(1/5)
BsrBI 27 GAGCGG CCGCTC(-3/-3)
BsrI 189 ACTGG ACTGG(1/-1)
BstYI 371 GGATCC [GA]^GATC[CT]
Bsu36I 624 CCTGAGG CC^T[ACGT]AGG
CelII 640 GCTGAGC GC^T[ACGT]AGC
CfoI 21 GCGC GCG^C
DsaI 611 CCATGG C^C[GA][CT]GG
Eco81I 624 CCTGAGG CC^T[ACGT]AGG
EcoRI 88 GAATTC G^AATTC
EcoT22I 351 ATGCAT ATGCA^T
HhaI 21 GCGC GCG^C
HinP1I 21 GCGC G^CGC
HincII 682 GTCAAC GT[CT]^[GA]AC
HindII 682 GTCAAC GT[CT]^[GA]AC
HindIII 473 AAGCTT A^AGCTT
MaeII 510 ACGT A^CGT
MflI 371 GGATCC [GA]^GATC[CT]
MseI 320 TTAA T^TAA
MslI 354 CATTCTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MwoI 631 GCCTTCTATGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NcoI 611 CCATGG C^CATGG
NsiI 351 ATGCAT ATGCA^T
SacI 601 GAGCTC GAGCT^C
ScaI 497 AGTACT AGT^ACT
SfaNI 517 GATGC GCATC(5/9)
SfcI 96 CTACAG C^T[GA][CT]AG
StuI 530 AGGCCT AGG^CCT
Tru9I 320 TTAA T^TAA
TspRI 188 CACTG CA[GC]TG[ACGT][ACGT]^
XhoII 371 GGATCC [GA]^GATC[CT]
XmnI 66 GAATCTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AciI 39 CCGC CCGC(-3/-1)
29 GCGG
AfaI 498 GTAC GT^AC
714 GTAC
BalI 2 TGGCCA TGG^CCA
524 TGGCCA
BanII 182 GGGCCC G[GA]GC[CT]^C
601 GAGCTC
BbvI 589 GCAGC GCAGC(8/12)
662 GCAGC
BcnI 180 CCGGG CC^[GC]GG
561 CCGGG
BmyI 182 GGGCCC G[AGT]GC[ACT]^C
601 GAGCTC
BpmI 439 CTGGAG CTGGAG(16/14)
670 CTGGAG
BsmFI 262 GTCCC GGGAC(10/14)
303 GTCCC
BsmI 450 GAATGC GAATGC(1/-1)
353 GCATTC
Bsp1286I 182 GGGCCC G[AGT]GC[ACT]^C
601 GAGCTC
BstXI 325 CCAGAAGTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
611 CCATGGTGCTGG
Cac8I 37 GCCCGC GC[ACGT]^[ACGT]GC
309 GCTTGC
DpnII 372 GATC ^GATC
384 GATC
DraII 182 GGGCCCC [GA]G^G[ACGT]CC[CT]
628 AGGGCCT
EaeI 2 TGGCCA [CT]^GGCC[GA]
524 TGGCCA
EcoO109I 182 GGGCCCC [GA]G^G[ACGT]CC[CT]
628 AGGGCCT
FauI 38 CCCGC CCCGC(4/6)
29 GCGGG
Fnu4HI 589 GCAGC GC^[ACGT]GC
662 GCAGC
HapII 180 CCGG C^CGG
561 CCGG
HpaII 180 CCGG C^CGG
561 CCGG
ItaI 589 GCAGC GC^[ACGT]GC
662 GCAGC
MboI 372 GATC ^GATC
384 GATC
MluNI 2 TGGCCA TGG^CCA
524 TGGCCA
MscI 2 TGGCCA TGG^CCA
524 TGGCCA
MspI 180 CCGG C^CGG
561 CCGG
NciI 180 CCGGG CC^[GC]GG
561 CCGGG
NdeII 372 GATC ^GATC
384 GATC
RsaI 498 GTAC GT^AC
714 GTAC
Sau3AI 372 GATC ^GATC
384 GATC
TseI 589 GCAGC G^C[AT]GC
662 GCAGC
Tsp509I 89 AATT ^AATT
678 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 26 enzymes) #
#########################################
AluI AlwI BsaJI BsiYI BslI
BssKI BstF5I BstNI Cfr13I DdeI
EcoRII EcoT14I FokI HaeIII HinfI
HphI MboII MnlI MvaI NlaIII
NlaIV PleI Sau96I ScrFI StyI
TfiI