Restriction map for
pFN21AB5930
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# NO CUT ENZYMES ( 125 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AcyI AflII AgeI AhdI
AocI Aor51HI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AviII
AvrII BalI BamHI BbeI BbrPI
BbvCI BciVI BclI BfrI BglI
BglII BlnI BsaAI BsaBI BsaHI
BsaWI BseAI BsgI BsiEI BsiWI
BsmBI BsmI BspDI BspEI BspHI
BspLU11I BsrDI BssHII BssSI Bst1107I
BstAPI BstBI BstYI BstZ17I Bsu36I
ClaI CpoI DraI DraIII DrdI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoRI EcoRV EcoT14I EcoT22I
FbaI FspI HgaI Hin1I HpaI
KasI KpnI KspI MamI MfeI
MflI MluI MluNI MroI MscI
MunI NarI NcoI NdeI NheI
NotI NruI NsiI NspV PacI
PinAI PmaCI PmeI PmlI PshBI
Psp1406I PvuI RcaI RsrII SacI
SacII SalI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SnaBI
SpeI Sse8387I SspI StyI SwaI
TfiI Tsp509I XbaI XhoII XmnI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AccII 5 CGCG CG^CG
AflIII 1463 ACACGT A^C[GA][CT]GT
Alw44I 878 GTGCAC G^TGCAC
AlwI 1101 GGATC GGATC(4/5)
ApaLI 878 GTGCAC G^TGCAC
AspHI 878 GTGCAC G[AT]GC[AT]^C
BanI 927 GGTGCC G^G[CT][GA]CC
BcgI 426 CGAAGACCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlpI 1079 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1079 GCTCAGC GC^T[ACGT]AGC
BsiHKAI 878 GTGCAC G[AT]GC[AT]^C
BsrBI 820 GAGCGG CCGCTC(-3/-3)
BstEII 1403 GGTCACC G^GT[ACGT]ACC
BstPI 1403 GGTCACC G^GT[ACGT]ACC
BstUI 5 CGCG CG^CG
CelII 1079 GCTCAGC GC^T[ACGT]AGC
CfoI 414 GCGC GCG^C
DsaI 964 CCACGG C^C[GA][CT]GG
EarI 166 GAAGAG CTCTTC(1/4)
EcoO65I 1403 GGTCACC G^GT[ACGT]ACC
FseI 1446 GGCCGGCC GGCCGG^CC
HaeII 413 GGCGCT [GA]GCGC^[CT]
HhaI 414 GCGC GCG^C
HinP1I 414 GCGC G^CGC
HincII 286 GTCAAC GT[CT]^[GA]AC
HindII 286 GTCAAC GT[CT]^[GA]AC
HindIII 457 AAGCTT A^AGCTT
Ksp632I 166 GAAGAG CTCTTC(1/4)
MseI 688 TTAA T^TAA
MslI 244 CACATGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 5 CGCG CG^CG
PaeR7I 579 CTCGAG C^TCGAG
PshAI 1266 GACCACAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SapI 166 GAAGAGC GCTCTTC(1/4)
SmaI 1528 CCCGGG CCC^GGG
SphI 225 GCATGC GCATG^C
StuI 1236 AGGCCT AGG^CCT
Tru9I 688 TTAA T^TAA
XcmI 896 CCAGCCTGGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 579 CTCGAG C^TCGAG
XmaCI 1528 CCCGGG C^CCGGG
XmaI 1528 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 20 enzymes) #
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AlwNI 1047 CAGCTTCTG CAG[ACGT][ACGT][ACGT]^CTG
1133 CAGTGACTG
ApaI 607 GGGCCC GGGCC^C
1318 GGGCCC
BsaI 491 GAGACC GGTCTC(1/5)
1416 GGTCTC
Bsp1407I 839 TGTACA T^GTACA
947 TGTACA
BsrGI 839 TGTACA T^GTACA
947 TGTACA
BstXI 318 CCAGGAGTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1159 CCATGCACCTGG
DpnII 525 GATC ^GATC
1102 GATC
EcoNI 837 CCTGTACAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1306 CCTGGGCTAGG
FauI 600 GCGGG CCCGC(4/6)
1432 GCGGG
MaeII 374 ACGT A^CGT
1465 ACGT
MboI 525 GATC ^GATC
1102 GATC
NaeI 357 GCCGGC GCC^GGC
1447 GCCGGC
NdeII 525 GATC ^GATC
1102 GATC
NgoMIV 357 GCCGGC G^CCGGC
1447 GCCGGC
NspI 225 GCATGC [GA]CATG^[CT]
443 ACATGC
Sau3AI 525 GATC ^GATC
1102 GATC
SmlI 537 CTCAAG C^T[CT][GA]AG
579 CTCGAG
SspBI 839 TGTACA T^GTACA
947 TGTACA
TaqI 580 TCGA T^CGA
1591 TCGA
TthHB8I 580 TCGA T^CGA
1591 TCGA
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# 3 OR MORE CUT ENZYMES ( 71 enzymes) #
#########################################
AciI AfaI AluI AspI AvaI
AvaII BanII BbsI BbvI BcnI
BfaI BmyI BpmI BpuAI BsaJI
BseRI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrFI BsrI
BssKI BstF5I BstNI Cac8I Cfr10I
Cfr13I DdeI DraII EaeI EcoO109I
EcoRII Fnu4HI FokI HaeIII HapII
HinfI HpaII HphI ItaI MaeI
MaeIII MboII MnlI MspA1I MspI
MvaI MwoI NciI NlaIII NlaIV
PflFI PflMI PleI PpuMI PstI
PvuII RsaI Sau96I ScrFI SfaNI
SfcI TseI Tsp45I TspRI Tth111I
Van91I