(Circular) (Six-base) MAPSORT of: pFN21AB5930.seq Check: 6864 from: 1 to: 6319 pFN21AB5930 6319 bp With 182 enzymes: SgfI * April 15, 2010 09:29 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2252 2590 2724 2252 Size: 338 134 5847 Fragments arranged by size: 5847 338 134 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4129 278 Size: 53 83 186 936 2593 2468 Fragments arranged by size: 2593 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3662 1937 Size: 1725 4594 Acc65I G'GTAC_C Cuts at: 3640 3640 Size: 6319 AclI AA'CG_TT Cuts at: 1563 4446 4819 1563 Size: 2883 373 3063 Fragments arranged by size: 3063 2883 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2198 2450 2474 2789 4382 5766 6034 1632 Size: 566 252 24 315 1593 1384 268 1917 Fragments arranged by size: 1917 1593 1384 566 315 268 252 24 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6122 AflIII A'CryG_T Cuts at: 1203 3468 5218 6195 1203 Size: 2265 1750 977 1327 Fragments arranged by size: 2265 1750 1327 977 AgeI A'CCGG_T Cuts at: 5150 5150 Size: 6319 AhdI GACnn_n'nnGTC Cuts at: 5048 5048 Size: 6319 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6319 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6319 AlwNI CAG_nnn'CTG Cuts at: 3057 3143 4062 5634 3057 Size: 86 919 1572 3742 Fragments arranged by size: 3742 1572 919 86 ApaI G_GGCC'C Cuts at: 2616 3327 2616 Size: 711 5608 ApaLI G'TGCA_C Cuts at: 2883 4376 5532 2883 Size: 1493 1156 3670 Fragments arranged by size: 3670 1493 1156 ApoI r'AATT_y Cuts at: 1493 3628 3854 4006 6115 1493 Size: 2135 226 152 2109 1697 Fragments arranged by size: 2135 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4873 160 Size: 872 3841 1606 Fragments arranged by size: 3841 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6319 AvaI C'yCGr_G Cuts at: 1870 1943 2584 3076 3533 3644 1870 Size: 73 641 492 457 111 4545 Fragments arranged by size: 4545 641 492 457 111 73 BamHI G'GATC_C Cuts at: 3649 4016 3649 Size: 367 5952 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2932 3640 5095 618 Size: 325 217 627 1145 708 1455 1842 Fragments arranged by size: 1842 1455 1145 708 627 325 217 BanII G_rGCy'C Cuts at: 1391 2616 2927 2978 3086 3234 3327 3443 3494 Size: 1225 311 51 108 148 93 116 51 Cuts at: 3494 3602 3638 1391 Size: 108 36 4072 Fragments arranged by size: 4072 1225 311 148 116 108 108 93 51 51 36 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2439 2737 2777 928 Size: 971 540 298 40 4470 Fragments arranged by size: 4470 971 540 298 40 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 2420 2454 4510 4544 1903 Size: 34 50 34 399 34 2056 34 3678 Fragments arranged by size: 3678 2056 399 50 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4211 5421 4211 Size: 1210 5109 BclI T'GATC_A Cuts at: 1548 1548 Size: 6319 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4930 136 Size: 107 122 71 631 605 3258 1525 Fragments arranged by size: 3258 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6314 6314 Size: 6319 BlpI GC'TnA_GC Cuts at: 3085 3736 3085 Size: 651 5668 Bme1580I G_kGCm'C Cuts at: 1790 2616 2887 2937 3045 3327 4380 5536 1790 Size: 826 271 50 108 282 1053 1156 2573 Fragments arranged by size: 2573 1156 1053 826 282 271 108 50 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6319 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2674 3388 4050 Size: 416 53 83 399 24 1257 714 662 Cuts at: 4050 5008 442 Size: 958 1753 Fragments arranged by size: 1753 1257 958 714 662 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6319 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2209 2953 3457 2209 Size: 744 504 5071 Fragments arranged by size: 5071 744 504 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2030 2078 2588 2665 2782 2884 2927 2941 Size: 791 48 510 77 117 102 43 14 Cuts at: 2941 2966 3248 3435 4979 1239 Size: 25 282 187 1544 2579 Fragments arranged by size: 2579 1544 791 510 282 187 117 102 77 48 43 25 14 Bpu10I CC'TnA_GC Cuts at: 2823 2823 Size: 6319 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2527 4444 5309 5607 2527 Size: 1917 865 298 3239 Fragments arranged by size: 3239 1917 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2490 3427 4982 882 Size: 617 991 937 1555 2219 Fragments arranged by size: 2219 1555 991 937 617 BsaAI yAC'GTr Cuts at: 493 1204 4110 493 Size: 711 2906 2702 Fragments arranged by size: 2906 2702 711 BsaBI GATnn'nnATC Cuts at: 6313 6313 Size: 6319 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4126 4508 5143 275 Size: 53 83 186 936 2593 382 635 1451 Fragments arranged by size: 2593 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4752 5150 5424 5571 5982 4752 Size: 398 274 147 411 5089 Fragments arranged by size: 5089 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6094 Fragments arranged by size: 6094 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2533 2549 2617 2702 2717 3113 1186 Size: 1347 16 68 85 15 396 4392 Fragments arranged by size: 4392 1347 396 85 68 16 15 BseYI C'CCAG_C Cuts at: 2016 2055 2135 2816 2911 3050 3202 3233 3383 Size: 39 80 681 95 139 152 31 150 Cuts at: 3383 5522 6018 2016 Size: 2139 496 2317 Fragments arranged by size: 2317 2139 681 496 152 150 139 95 80 39 31 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5746 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3769 4530 5558 1540 Size: 275 186 1768 761 1028 2301 Fragments arranged by size: 2301 1768 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2887 4380 4465 5536 2887 Size: 1493 85 1071 3670 Fragments arranged by size: 3670 1493 1071 85 BsmI GAATG_Cn' Cuts at: 3830 3923 3830 Size: 93 6226 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2616 2887 2927 2937 2978 3045 3086 Size: 399 826 271 40 10 41 67 41 Cuts at: 3086 3234 3327 3443 3494 3602 3638 4380 4465 Size: 148 93 116 51 108 36 742 85 Cuts at: 4465 5536 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 826 742 399 271 148 116 108 93 85 67 51 41 41 40 36 10 BspEI T'CCGG_A Cuts at: 5982 5982 Size: 6319 BspHI T'CATG_A Cuts at: 4208 5938 4208 Size: 1730 4589 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2252 2590 2724 3674 844 Size: 1408 338 134 950 3489 Fragments arranged by size: 3489 1408 950 338 134 BsrBI CCG'CTC Cuts at: 1394 1823 2827 4206 1394 Size: 429 1004 1379 3507 Fragments arranged by size: 3507 1379 1004 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4814 4988 65 Size: 1183 3566 174 1396 Fragments arranged by size: 3566 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2362 3405 3452 4963 5150 1700 Size: 110 552 1043 47 1511 187 2869 Fragments arranged by size: 2869 1511 1043 552 187 110 47 BsrGI T'GTAC_A Cuts at: 96 2844 2952 96 Size: 2748 108 3463 Fragments arranged by size: 3463 2748 108 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6319 BssSI C'ACGA_G Cuts at: 4379 5391 4379 Size: 1012 5307 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6319 BstBI TT'CG_AA Cuts at: 5123 5123 Size: 6319 BstEII G'GTnAC_C Cuts at: 1184 3408 1184 Size: 2224 4095 BstXI CCAn_nnnn'nTGG Cuts at: 2330 3171 2330 Size: 841 5478 BstYI r'GATC_y Cuts at: 1972 3649 4016 4401 4418 5870 5956 5968 6314 Size: 1677 367 385 17 1452 86 12 346 Cuts at: 6314 1972 Size: 1977 Fragments arranged by size: 1977 1677 1452 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2969 513 Size: 552 1904 3863 Fragments arranged by size: 3863 1904 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5580 Fragments arranged by size: 5580 683 56 BtsI GCAGTG_nn' Cuts at: 3835 4629 4649 3835 Size: 794 20 5505 Fragments arranged by size: 5505 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6319 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6284 DraI TTT'AAA Cuts at: 3623 3623 Size: 6319 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5326 809 Size: 507 4010 1802 Fragments arranged by size: 4010 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2271 2979 3059 Size: 54 1425 50 275 18 441 708 80 Cuts at: 3059 3110 3403 3450 3766 4655 6284 8 Size: 51 293 47 316 889 1629 43 Fragments arranged by size: 1629 1425 889 708 441 316 293 275 80 54 51 50 47 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3766 1537 Size: 275 1954 4090 Fragments arranged by size: 4090 1954 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2166 4249 1353 Size: 813 2083 3423 Fragments arranged by size: 3423 2083 813 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3554 3576 4906 5280 5426 387 Size: 1064 138 1965 22 1330 374 146 1280 Fragments arranged by size: 1965 1330 1280 1064 374 146 138 22 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2030 2078 2198 2450 2474 2588 2665 Size: 393 398 48 120 252 24 114 77 Cuts at: 2665 2782 2789 2884 2927 2941 2966 3248 3435 Size: 117 7 95 43 14 25 282 187 Cuts at: 3435 4382 4979 5766 6034 1239 Size: 947 597 787 268 1524 Fragments arranged by size: 1524 947 787 597 398 393 282 268 252 187 120 117 114 95 77 48 43 25 24 14 7 EcoNI CCTnn'n_nnAGG Cuts at: 2846 3315 2846 Size: 469 5850 EcoO109I rG'GnC_Cy Cuts at: 2612 2640 2915 3250 3323 3324 3545 2612 Size: 28 275 335 73 1 221 5386 Fragments arranged by size: 5386 335 275 221 73 28 1 EcoRI G'AATT_C Cuts at: 3628 4006 3628 Size: 378 5941 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3070 3070 Size: 6319 FseI GG_CCGG'CC Cuts at: 3456 3456 Size: 6319 FspI TGC'GCA Cuts at: 4825 4825 Size: 6319 HaeII r_GCGC'y Cuts at: 1678 2422 5466 1678 Size: 744 3044 2531 Fragments arranged by size: 3044 2531 744 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4960 4992 5034 5066 1373 Size: 32 135 32 3388 32 42 32 2626 Fragments arranged by size: 3388 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2293 3663 3909 669 Size: 988 281 355 1370 246 3079 Fragments arranged by size: 3079 1370 988 355 281 246 HindIII A'AGCT_T Cuts at: 748 2462 748 Size: 1714 4605 HpaI GTT'AAC Cuts at: 3909 3909 Size: 6319 KpnI G_GTAC'C Cuts at: 3644 3644 Size: 6319 MfeI C'AATT_G Cuts at: 3918 3918 Size: 6319 MluI A'CGCG_T Cuts at: 6195 6195 Size: 6319 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5433 5617 1305 Size: 4128 184 2007 Fragments arranged by size: 4128 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4497 Fragments arranged by size: 4497 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2253 4277 4636 4795 518 Size: 648 1087 2024 359 159 2042 Fragments arranged by size: 2042 2024 1087 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2859 2958 3183 3302 3735 4412 5560 5805 Size: 1253 99 225 119 433 677 1148 245 Cuts at: 5805 1606 Size: 2120 Fragments arranged by size: 2120 1253 1148 677 433 245 225 119 99 NaeI GCC'GGC Cuts at: 2364 3454 2364 Size: 1090 5229 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5767 NdeI CA'TA_TG Cuts at: 387 387 Size: 6319 NgoMIV G'CCGG_C Cuts at: 2362 3452 2362 Size: 1090 5229 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6319 NotI GC'GGCC_GC Cuts at: 3766 3766 Size: 6319 NspI r_CATG'y Cuts at: 1132 1229 2234 2452 3677 5222 1132 Size: 97 1005 218 1225 1545 2229 Fragments arranged by size: 2229 1545 1225 1005 218 97 PasI CC'CwG_GG Cuts at: 1348 2502 3311 3328 3396 1348 Size: 1154 809 17 68 4271 Fragments arranged by size: 4271 1154 809 68 17 PciI A'CATG_T Cuts at: 5218 5218 Size: 6319 PflMI CCAn_nnn'nTGG Cuts at: 1108 2269 3057 3574 1108 Size: 1161 788 517 3853 Fragments arranged by size: 3853 1161 788 517 PfoI T'CCnGG_A Cuts at: 2112 3339 2112 Size: 1227 5092 PmeI GTTT'AAAC Cuts at: 3623 3623 Size: 6319 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4388 4420 5901 5933 4388 Size: 32 1481 32 4774 Fragments arranged by size: 4774 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2640 2915 3250 3545 2640 Size: 275 335 295 5414 Fragments arranged by size: 5414 335 295 275 PshAI GACnn'nnGTC Cuts at: 1613 3275 1613 Size: 1662 4657 PsiI TTA'TAA Cuts at: 3889 3889 Size: 6319 PspOMI G'GGCC_C Cuts at: 2612 3323 2612 Size: 711 5608 PspXI vC'TCGA_Gb Cuts at: 2584 2584 Size: 6319 PstI C_TGCA'G Cuts at: 830 2201 2481 2601 2858 2994 3182 3503 3671 Size: 1371 280 120 257 136 188 321 168 Cuts at: 3671 830 Size: 3478 Fragments arranged by size: 3478 1371 321 280 257 188 168 136 120 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6319 PvuII CAG'CTG Cuts at: 2958 3183 3302 2958 Size: 225 119 5975 Fragments arranged by size: 5975 225 119 SalI G'TCGA_C Cuts at: 1936 3661 1936 Size: 1725 4594 SanDI GG'GwC_CC Cuts at: 2915 2915 Size: 6319 SapI GCTCTTCn'nnn_ Cuts at: 2166 2166 Size: 6319 SbfI CC_TGCA'GG Cuts at: 3671 3671 Size: 6319 ScaI AGT'ACT Cuts at: 4567 6279 4567 Size: 1712 4607 SfcI C'TryA_G Cuts at: 826 1045 2197 2477 2597 2854 2990 3178 3499 Size: 219 1152 280 120 257 136 188 321 Cuts at: 3499 3667 4802 5483 5674 826 Size: 168 1135 681 191 1471 Fragments arranged by size: 1471 1152 1135 681 321 280 257 219 191 188 168 136 120 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6319 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6319 SmaI CCC'GGG Cuts at: 1872 3535 3646 1872 Size: 1663 111 4545 Fragments arranged by size: 4545 1663 111 SmlI C'TyrA_G Cuts at: 820 1017 1943 2542 2584 4423 5324 5586 820 Size: 197 926 599 42 1839 901 262 1553 Fragments arranged by size: 1839 1553 926 901 599 262 197 42 SnaBI TAC'GTA Cuts at: 493 4110 493 Size: 3617 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6319 SphI G_CATG'C Cuts at: 1132 2234 3677 1132 Size: 1102 1443 3774 Fragments arranged by size: 3774 1443 1102 SspI AAT'ATT Cuts at: 5 52 4243 5 Size: 47 4191 2081 Fragments arranged by size: 4191 2081 47 StuI AGG'CCT Cuts at: 1517 3243 1517 Size: 1726 4593 StyI C'CwwG_G Cuts at: 513 1065 3758 513 Size: 552 2693 3074 Fragments arranged by size: 3074 2693 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2866 2870 2979 3562 4346 4363 4516 Size: 1255 1286 4 109 583 784 17 153 Cuts at: 4516 4701 5146 325 Size: 185 445 1498 Fragments arranged by size: 1498 1286 1255 784 583 445 185 153 109 17 4 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2481 2844 2952 Size: 274 80 33 51 1443 504 363 108 Cuts at: 2952 4565 6277 96 Size: 1613 1712 138 Fragments arranged by size: 1712 1613 1443 504 363 274 138 108 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2632 2664 6279 6311 2632 Size: 32 3615 32 2640 Fragments arranged by size: 3615 2640 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2072 2663 2680 1316 Size: 756 591 17 4955 Fragments arranged by size: 4955 756 591 17 XbaI T'CTAG_A Cuts at: 3655 3655 Size: 6319 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2908 2908 Size: 6319 XhoI C'TCGA_G Cuts at: 1943 2584 1943 Size: 641 5678 XmaI C'CCGG_G Cuts at: 1870 3533 3644 1870 Size: 1663 111 4545 Fragments arranged by size: 4545 1663 111 XmnI GAAnn'nnTTC Cuts at: 4448 4448 Size: 6319 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4127 276 Size: 53 83 186 936 2593 2468 Fragments arranged by size: 2593 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FseI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SalI SanDI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV FspAI KasI NarI NruI NsiI PacI PmlI PsrI RsrII SacI SacII SexAI SfiI SfoI SrfI SwaI