Restriction map for
pFN21AB6663
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# NO CUT ENZYMES ( 127 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
Alw44I AocI Aor51HI ApaI ApaLI
AscI Asp718I AspHI AspI AviII
AvrII BamHI BanI BbeI BbrPI
BbsI BbvCI BcgI BfaI BfrI
BglII BlnI BpuAI BsaBI BsaHI
BsaI BsaWI BseAI BsgI BsiEI
BsiHKAI BsiWI BsmBI Bsp1407I BspDI
BspEI BspLU11I BspMI BsrDI BsrFI
BsrGI BssHII BssSI Bst1107I BstBI
BstUI BstZ17I Bsu36I Cfr10I ClaI
CpoI DraI EagI EarI EclXI
Eco47III Eco52I Eco81I EcoRV EcoT22I
FseI FspI HgaI Hin1I HindIII
HpaI KasI KpnI Ksp632I KspI
MaeI MamI MluI MroI MvnI
NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PflFI PinAI PmaCI PmeI PmlI
PshAI Psp1406I PvuI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI Sse8387I SspBI
SspI StuI SwaI Tth111I XbaI
XmaCI XmaI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AcsI 178 GAATTC [GA]^AATT[CT]
AhdI 110 GACCAGTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwI 1075 GGATC GGATC(4/5)
AlwNI 980 CAGGCTCTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 178 GAATTC [GA]^AATT[CT]
AseI 439 ATTAAT AT^TAAT
AsnI 439 ATTAAT AT^TAAT
AspEI 110 GACCAGTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI 150 TGGCCA TGG^CCA
BanII 752 GAGCCC G[GA]GC[CT]^C
BclI 227 TGATCA T^GATCA
BglI 944 GCCCTCATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 563 GCTCAGC GC^T[ACGT]AGC
BmyI 752 GAGCCC G[AGT]GC[ACT]^C
Bpu1102I 563 GCTCAGC GC^T[ACGT]AGC
BsaAI 46 TACGTG [CT]AC^GT[GA]
BsmAI 918 GTCTC GTCTC(1/5)
BsmFI 401 GGGAC GGGAC(10/14)
BsmI 1019 GAATGC GAATGC(1/-1)
Bsp1286I 752 GAGCCC G[AGT]GC[ACT]^C
BspHI 218 TCATGA T^CATGA
BstAPI 1110 GCATGGTATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 1087 GGTCACC G^GT[ACGT]ACC
BstPI 1087 GGTCACC G^GT[ACGT]ACC
BstYI 1075 GGATCT [GA]^GATC[CT]
CelII 563 GCTCAGC GC^T[ACGT]AGC
DraIII 890 CACCGGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 851 GACATCCTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 110 GACCAGTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 1087 GGTCACC G^GT[ACGT]ACC
EcoRI 178 GAATTC G^AATTC
FbaI 227 TGATCA T^GATCA
HincII 118 GTCAAC GT[CT]^[GA]AC
HindII 118 GTCAAC GT[CT]^[GA]AC
MaeII 47 ACGT A^CGT
MboII 193 GAAGA GAAGA(8/7)
MfeI 473 CAATTG C^AATTG
MflI 1075 GGATCT [GA]^GATC[CT]
MluNI 150 TGGCCA TGG^CCA
MscI 150 TGGCCA TGG^CCA
MunI 473 CAATTG C^AATTG
PaeR7I 1241 CTCGAG C^TCGAG
PshBI 439 ATTAAT AT^TAAT
PstI 809 CTGCAG CTGCA^G
PvuII 625 CAGCTG CAG^CTG
RcaI 218 TCATGA T^CATGA
SfcI 809 CTGCAG C^T[GA][CT]AG
TfiI 27 GAATC G^A[AT]TC
XhoI 1241 CTCGAG C^TCGAG
XhoII 1075 GGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AfaI 45 GTAC GT^AC
495 GTAC
Asp700I 91 GAACTGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
574 GAACGACTTC
AvaI 1058 CCCGAG C^[CT]CG[GA]G
1241 CTCGAG
BciVI 767 GGATAC GTATCC(6/5)
1150 GGATAC
BpmI 100 CTCCAG CTGGAG(16/14)
749 CTGGAG
BsoBI 1058 CCCGAG C^[CT]CG[GA]G
1241 CTCGAG
BsrBI 598 CCGCTC CCGCTC(-3/-3)
22 GAGCGG
BstXI 159 CCAAGGCTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
662 CCAATCCTGTGG
CfoI 362 GCGC GCG^C
555 GCGC
EaeI 150 TGGCCA [CT]^GGCC[GA]
1225 CGGCCA
EcoNI 406 CCTGACACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
697 CCTCCACCAGG
HaeII 361 AGCGCC [GA]GCGC^[CT]
554 AGCGCC
HhaI 362 GCGC GCG^C
555 GCGC
HinP1I 362 GCGC G^CGC
555 GCGC
MseI 212 TTAA T^TAA
440 TTAA
MslI 123 CACCCACATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
219 CATGAACATG
PleI 541 GACTC GAGTC(4/5)
143 GAGTC
PpuMI 401 GGGACCC [GA]G^G[AT]CC[CT]
1095 AGGTCCT
RsaI 45 GTAC GT^AC
495 GTAC
SmlI 183 CTTGAG C^T[CT][GA]AG
1241 CTCGAG
SphI 707 GCATGC GCATG^C
1049 GCATGC
TaqI 1242 TCGA T^CGA
1254 TCGA
Tru9I 212 TTAA T^TAA
440 TTAA
Tsp45I 145 GTCAC ^GT[GC]AC
1088 GTCAC
TthHB8I 1242 TCGA T^CGA
1254 TCGA
XmnI 91 GAACTGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
574 GAACGACTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 55 enzymes) #
#########################################
AciI AluI AvaII BbvI BcnI
BsaJI BseRI BsiYI BslI BsrI
BssKI BstF5I BstNI Cac8I Cfr13I
DdeI DpnII DraII DsaI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HinfI HpaII HphI
ItaI MaeIII MboI MnlI MspA1I
MspI MvaI MwoI NciI NcoI
NdeII NlaIII NlaIV NspI PflMI
Sau3AI Sau96I ScrFI SfaNI StyI
TseI Tsp509I TspRI Van91I XcmI