(Circular) (Six-base) MAPSORT of: pFN21AB6663.seq Check: 2711 from: 1 to: 5977 pFN21AB6663 5977 bp With 182 enzymes: SgfI * October 20, 2009 10:58 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3787 278 Size: 53 83 186 936 2251 2468 Fragments arranged by size: 2468 2251 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3320 1937 Size: 1383 4594 Acc65I G'GTAC_C Cuts at: 3298 3298 Size: 5977 AclI AA'CG_TT Cuts at: 1563 4104 4477 1563 Size: 2541 373 3063 Fragments arranged by size: 3063 2541 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4040 5424 5692 1632 Size: 2408 1384 268 1917 Fragments arranged by size: 2408 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5780 AflIII A'CryG_T Cuts at: 1203 4876 5853 1203 Size: 3673 977 1327 Fragments arranged by size: 3673 1327 977 AgeI A'CCGG_T Cuts at: 4808 4808 Size: 5977 AhdI GACnn_n'nnGTC Cuts at: 2120 4706 2120 Size: 2586 3391 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5977 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5977 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2088 2120 2591 2623 2088 Size: 32 471 32 5442 Fragments arranged by size: 5442 471 32 32 AlwNI CAG_nnn'CTG Cuts at: 2990 3720 5292 2990 Size: 730 1572 3675 Fragments arranged by size: 3675 1572 730 ApaLI G'TGCA_C Cuts at: 4034 5190 4034 Size: 1156 4821 ApoI r'AATT_y Cuts at: 1493 2183 3286 3512 3664 5773 1493 Size: 690 1103 226 152 2109 1697 Fragments arranged by size: 2109 1697 1103 690 226 152 AseI AT'TA_AT Cuts at: 160 1032 2445 4531 160 Size: 872 1413 2086 1606 Fragments arranged by size: 2086 1606 1413 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5977 AvaI C'yCGr_G Cuts at: 1870 1943 3063 3246 3302 1870 Size: 73 1120 183 56 4545 Fragments arranged by size: 4545 1120 183 73 56 BamHI G'GATC_C Cuts at: 3307 3674 3307 Size: 367 5610 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3298 4753 618 Size: 325 217 627 1511 1455 1842 Fragments arranged by size: 1842 1511 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2761 3296 1391 Size: 1370 535 4072 Fragments arranged by size: 4072 1370 535 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5006 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4168 4202 1903 Size: 34 2231 34 3678 Fragments arranged by size: 3678 2231 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2766 3149 3869 5079 2766 Size: 383 720 1210 3664 Fragments arranged by size: 3664 1210 720 383 BclI T'GATC_A Cuts at: 1548 2232 1548 Size: 684 5293 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2955 4588 136 Size: 107 122 71 631 605 1283 1633 1525 Fragments arranged by size: 1633 1525 1283 631 605 122 107 71 BglII A'GATC_T Cuts at: 5972 5972 Size: 5977 BlpI GC'TnA_GC Cuts at: 2569 3394 2569 Size: 825 5152 Bme1580I G_kGCm'C Cuts at: 1790 4038 5194 1790 Size: 2248 1156 2573 Fragments arranged by size: 2573 2248 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5977 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2735 3708 4666 Size: 416 53 83 399 24 1318 973 958 Cuts at: 4666 442 Size: 1753 Fragments arranged by size: 1753 1318 973 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5977 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3284 3284 Size: 5977 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2090 2775 4637 1239 Size: 851 685 1862 2579 Fragments arranged by size: 2579 1862 851 685 Bpu10I CC'TnA_GC Cuts at: 2959 2959 Size: 5977 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2209 4102 4967 5265 2209 Size: 1893 865 298 2921 Fragments arranged by size: 2921 1893 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4640 882 Size: 617 3141 2219 Fragments arranged by size: 3141 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2053 3768 493 Size: 711 849 1715 2702 Fragments arranged by size: 2702 1715 849 711 BsaBI GATnn'nnATC Cuts at: 5971 5971 Size: 5977 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3784 4166 4801 275 Size: 53 83 186 936 2251 382 635 1451 Fragments arranged by size: 2251 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4410 4808 5082 5229 5640 4410 Size: 398 274 147 411 4747 Fragments arranged by size: 4747 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2088 2118 2591 2621 3151 Size: 30 165 30 685 30 473 30 530 Cuts at: 3151 3181 1178 Size: 30 3974 Fragments arranged by size: 3974 685 530 473 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2640 2709 3033 3123 1186 Size: 1454 69 324 90 4040 Fragments arranged by size: 4040 1454 324 90 69 BseYI C'CCAG_C Cuts at: 2779 5180 5676 2779 Size: 2401 496 3080 Fragments arranged by size: 3080 2401 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5404 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3427 4188 5216 1540 Size: 275 186 1426 761 1028 2301 Fragments arranged by size: 2301 1426 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4038 4123 5194 4038 Size: 85 1071 4821 Fragments arranged by size: 4821 1071 85 BsmI GAATG_Cn' Cuts at: 3030 3488 3581 3030 Size: 458 93 5426 Fragments arranged by size: 5426 458 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2761 3296 4038 4123 5194 1391 Size: 399 971 535 742 85 1071 2174 Fragments arranged by size: 2174 1071 971 742 535 399 85 BspEI T'CCGG_A Cuts at: 5640 5640 Size: 5977 BspHI T'CATG_A Cuts at: 2223 3866 5596 2223 Size: 1643 1730 2604 Fragments arranged by size: 2604 1730 1643 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3332 844 Size: 2488 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2029 2605 3864 1394 Size: 429 206 576 1259 3507 Fragments arranged by size: 3507 1259 576 429 206 BsrDI GCAATG_nn' Cuts at: 65 1248 4472 4646 65 Size: 1183 3224 174 1396 Fragments arranged by size: 3224 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4621 4808 1700 Size: 110 2811 187 2869 Fragments arranged by size: 2869 2811 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5977 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5977 BssSI C'ACGA_G Cuts at: 4037 5049 4037 Size: 1012 4965 BstAPI GCAn_nnn'nTGC Cuts at: 794 3121 794 Size: 2327 3650 BstBI TT'CG_AA Cuts at: 4781 4781 Size: 5977 BstEII G'GTnAC_C Cuts at: 1184 3092 1184 Size: 1908 4069 BstXI CCAn_nnnn'nTGG Cuts at: 2171 2674 2171 Size: 503 5474 BstYI r'GATC_y Cuts at: 1972 3080 3307 3674 4059 4076 5528 5614 5626 Size: 1108 227 367 385 17 1452 86 12 Cuts at: 5626 5972 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1108 385 367 346 227 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3273 3273 Size: 5977 BtgI C'CryG_G Cuts at: 513 1065 2004 2563 2944 3087 3151 513 Size: 552 939 559 381 143 64 3339 Fragments arranged by size: 3339 939 559 552 381 143 64 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5238 Fragments arranged by size: 5238 683 56 BtsI GCAGTG_nn' Cuts at: 3493 4287 4307 3493 Size: 794 20 5163 Fragments arranged by size: 5163 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5977 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5942 DraI TTT'AAA Cuts at: 3281 3281 Size: 5977 DraIII CAC_nnn'GTG Cuts at: 2900 2900 Size: 5977 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2862 4984 809 Size: 507 1546 2122 1802 Fragments arranged by size: 2122 1802 1546 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2155 3230 3424 Size: 54 1425 50 275 18 325 1075 194 Cuts at: 3424 4313 5942 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1075 889 325 275 194 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3424 1537 Size: 275 1612 4090 Fragments arranged by size: 4090 1612 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3907 1353 Size: 2554 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4564 4938 5084 387 Size: 1064 138 2975 374 146 1280 Fragments arranged by size: 2975 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2090 2775 4040 4637 5424 5692 1239 Size: 393 458 685 1265 597 787 268 1524 Fragments arranged by size: 1524 1265 787 685 597 458 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2415 2706 2415 Size: 291 5686 EcoO109I rG'GnC_Cy Cuts at: 2059 2407 3068 3101 3200 3263 2059 Size: 348 661 33 99 63 4773 Fragments arranged by size: 4773 661 348 99 63 33 EcoRI G'AATT_C Cuts at: 2183 3286 3664 2183 Size: 1103 378 4496 Fragments arranged by size: 4496 1103 378 FspI TGC'GCA Cuts at: 4483 4483 Size: 5977 HaeII r_GCGC'y Cuts at: 1678 2370 2563 5124 1678 Size: 692 193 2561 2531 Fragments arranged by size: 2561 2531 692 193 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2106 2138 2896 2928 4618 Size: 32 135 32 534 32 758 32 1690 Cuts at: 4618 4650 4692 4724 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1690 758 534 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2125 3321 3567 669 Size: 988 281 187 1196 246 3079 Fragments arranged by size: 3079 1196 988 281 246 187 HindIII A'AGCT_T Cuts at: 748 748 Size: 5977 HpaI GTT'AAC Cuts at: 3567 3567 Size: 5977 KpnI G_GTAC'C Cuts at: 3302 3302 Size: 5977 MfeI C'AATT_G Cuts at: 2478 3576 2478 Size: 1098 4879 MluI A'CGCG_T Cuts at: 5853 5853 Size: 5977 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5091 5275 1305 Size: 3786 184 2007 Fragments arranged by size: 3786 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2157 10 Size: 54 1425 343 325 3830 Fragments arranged by size: 3830 1425 343 325 54 MslI CAynn'nnrTG Cuts at: 518 1166 2132 2228 3935 4294 4453 518 Size: 648 966 96 1707 359 159 2042 Fragments arranged by size: 2042 1707 966 648 359 159 96 MspA1I CmG'CkG Cuts at: 1606 2086 2632 3196 3393 4070 5218 5463 1606 Size: 480 546 564 197 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 564 546 480 245 197 NcoI C'CATG_G Cuts at: 513 1065 2004 2563 2944 3087 3151 513 Size: 552 939 559 381 143 64 3339 Fragments arranged by size: 3339 939 559 552 381 143 64 NdeI CA'TA_TG Cuts at: 387 387 Size: 5977 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5977 NotI GC'GGCC_GC Cuts at: 3424 3424 Size: 5977 NspI r_CATG'y Cuts at: 1132 1229 2137 2716 3058 3335 4880 1132 Size: 97 908 579 342 277 1545 2229 Fragments arranged by size: 2229 1545 908 579 342 277 97 PasI CC'CwG_GG Cuts at: 1348 2846 1348 Size: 1498 4479 PciI A'CATG_T Cuts at: 4876 4876 Size: 5977 PflMI CCAn_nnn'nTGG Cuts at: 1108 2753 2900 2990 3020 3185 1108 Size: 1645 147 90 30 165 3900 Fragments arranged by size: 3900 1645 165 147 90 30 PfoI T'CCnGG_A Cuts at: 2752 3034 2752 Size: 282 5695 PmeI GTTT'AAAC Cuts at: 3281 3281 Size: 5977 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2088 2120 2591 2623 4046 4078 5559 5591 2088 Size: 32 471 32 1423 32 1481 32 2474 Fragments arranged by size: 2474 1481 1423 471 32 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2407 3101 2407 Size: 694 5283 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5977 PsiI TTA'TAA Cuts at: 3547 3547 Size: 5977 PspXI vC'TCGA_Gb Cuts at: 3246 3246 Size: 5977 PstI C_TGCA'G Cuts at: 830 2818 3329 830 Size: 1988 511 3478 Fragments arranged by size: 3478 1988 511 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5977 PvuII CAG'CTG Cuts at: 2632 2632 Size: 5977 SalI G'TCGA_C Cuts at: 1936 3319 1936 Size: 1383 4594 SanDI GG'GwC_CC Cuts at: 2407 2407 Size: 5977 SbfI CC_TGCA'GG Cuts at: 3329 3329 Size: 5977 ScaI AGT'ACT Cuts at: 4225 5937 4225 Size: 1712 4265 SfcI C'TryA_G Cuts at: 826 1045 2814 3325 4460 5141 5332 826 Size: 219 1769 511 1135 681 191 1471 Fragments arranged by size: 1769 1471 1135 681 511 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5977 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5977 SmaI CCC'GGG Cuts at: 1872 3304 1872 Size: 1432 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2188 3246 4081 4982 5244 820 Size: 197 926 245 1058 835 901 262 1553 Fragments arranged by size: 1553 1058 926 901 835 262 245 197 SnaBI TAC'GTA Cuts at: 493 3768 493 Size: 3275 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5977 SphI G_CATG'C Cuts at: 1132 2716 3058 3335 1132 Size: 1584 342 277 3774 Fragments arranged by size: 3774 1584 342 277 SspI AAT'ATT Cuts at: 5 52 3901 5 Size: 47 3849 2081 Fragments arranged by size: 3849 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5977 StyI C'CwwG_G Cuts at: 513 1065 2004 2164 2550 2563 2944 3087 3151 Size: 552 939 160 386 13 381 143 64 Cuts at: 3151 3416 513 Size: 265 3074 Fragments arranged by size: 3074 939 552 386 381 265 160 143 64 13 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2144 2901 3111 3229 4004 4021 4174 Size: 1255 564 757 210 118 775 17 153 Cuts at: 4174 4359 4804 325 Size: 185 445 1498 Fragments arranged by size: 1498 1255 775 757 564 445 210 185 153 118 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4223 5935 96 Size: 274 80 33 51 1443 2246 1712 138 Fragments arranged by size: 2246 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3151 3183 5937 5969 3151 Size: 32 2754 32 3159 Fragments arranged by size: 3159 2754 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5977 XbaI T'CTAG_A Cuts at: 3313 3313 Size: 5977 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2050 2951 3158 2050 Size: 901 207 4869 Fragments arranged by size: 4869 901 207 XhoI C'TCGA_G Cuts at: 1943 3246 1943 Size: 1303 4674 XmaI C'CCGG_G Cuts at: 1870 3302 1870 Size: 1432 4545 XmnI GAAnn'nnTTC Cuts at: 2100 2583 4106 2100 Size: 483 1523 3971 Fragments arranged by size: 3971 1523 483 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3785 276 Size: 53 83 186 936 2251 2468 Fragments arranged by size: 2468 2251 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspXI PstI PvuI PvuII SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BsiWI BsmBI BstZ17I EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PsrI RsrII SacI SacII SapI SexAI SfiI SfoI SrfI SwaI