Restriction map for
pFN21AB6663
(Linear) (Six-base) MAPSORT of: KIBB6663.seq Check: 2611 from: 1 to: 1272
KIBB6663.orf
With 182 enzymes: SgfI *
October 7, 2009 14:22 ..
AhdI GACnn_n'nnGTC
Cuts at: 0 115 1272
Size: 115 1157
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 83 115 586 618 1272
Size: 83 32 471 32 654
Fragments arranged by size:
654 471 83 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 985 1272
Size: 985 287
ApoI r'AATT_y
Cuts at: 0 178 1272
Size: 178 1094
AseI AT'TA_AT
Cuts at: 0 440 1272
Size: 440 832
AvaI C'yCGr_G
Cuts at: 0 1058 1241 1272
Size: 1058 183 31
Fragments arranged by size:
1058 183 31
BanII G_rGCy'C
Cuts at: 0 756 1272
Size: 756 516
BciVI GTATCCnnnnn_n'
Cuts at: 0 761 1144 1272
Size: 761 383 128
Fragments arranged by size:
761 383 128
BclI T'GATC_A
Cuts at: 0 227 1272
Size: 227 1045
BglI GCCn_nnn'nGGC
Cuts at: 0 950 1272
Size: 950 322
BlpI GC'TnA_GC
Cuts at: 0 564 1272
Size: 564 708
BmrI ACTGGGnnnn_n'
Cuts at: 0 730 1272
Size: 730 542
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 85 770 1272
Size: 85 685 502
Fragments arranged by size:
685 502 85
Bpu10I CC'TnA_GC
Cuts at: 0 954 1272
Size: 954 318
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 204 1272
Size: 204 1068
BsaAI yAC'GTr
Cuts at: 0 48 1272
Size: 48 1224
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 83 113 586 616 1146 1176 1272
Size: 83 30 473 30 530 30 96
Fragments arranged by size:
530 473 96 83 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 635 704 1028 1118 1272
Size: 635 69 324 90 154
Fragments arranged by size:
635 324 154 90 69
BseYI C'CCAG_C
Cuts at: 0 774 1272
Size: 774 498
BsmI GAATG_Cn'
Cuts at: 0 1025 1272
Size: 1025 247
Bsp1286I G_dGCh'C
Cuts at: 0 756 1272
Size: 756 516
BspHI T'CATG_A
Cuts at: 0 218 1272
Size: 218 1054
BsrBI CCG'CTC
Cuts at: 0 24 600 1272
Size: 24 576 672
Fragments arranged by size:
672 576 24
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1116 1272
Size: 1116 156
BstEII G'GTnAC_C
Cuts at: 0 1087 1272
Size: 1087 185
BstXI CCAn_nnnn'nTGG
Cuts at: 0 166 669 1272
Size: 166 503 603
Fragments arranged by size:
603 503 166
BstYI r'GATC_y
Cuts at: 0 1075 1272
Size: 1075 197
BtgI C'CryG_G
Cuts at: 0 558 939 1082 1146 1272
Size: 558 381 143 64 126
Fragments arranged by size:
558 381 143 126 64
DraIII CAC_nnn'GTG
Cuts at: 0 895 1272
Size: 895 377
DrdI GACnn_nn'nnGTC
Cuts at: 0 857 1272
Size: 857 415
EaeI y'GGCC_r
Cuts at: 0 150 1225 1272
Size: 150 1075 47
Fragments arranged by size:
1075 150 47
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 85 770 1272
Size: 85 685 502
Fragments arranged by size:
685 502 85
EcoNI CCTnn'n_nnAGG
Cuts at: 0 410 701 1272
Size: 410 291 571
Fragments arranged by size:
571 410 291
EcoO109I rG'GnC_Cy
Cuts at: 0 54 402 1063 1096 1195 1258 1272
Size: 54 348 661 33 99 63 14
Fragments arranged by size:
661 348 99 63 54 33 14
EcoRI G'AATT_C
Cuts at: 0 178 1272
Size: 178 1094
HaeII r_GCGC'y
Cuts at: 0 365 558 1272
Size: 365 193 714
Fragments arranged by size:
714 365 193
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 101 133 891 923 1272
Size: 101 32 758 32 349
Fragments arranged by size:
758 349 101 32 32
HincII GTy'rAC
Cuts at: 0 120 1272
Size: 120 1152
MfeI C'AATT_G
Cuts at: 0 473 1272
Size: 473 799
MscI TGG'CCA
Cuts at: 0 152 1272
Size: 152 1120
MslI CAynn'nnrTG
Cuts at: 0 127 223 1272
Size: 127 96 1049
Fragments arranged by size:
1049 127 96
MspA1I CmG'CkG
Cuts at: 0 81 627 1191 1272
Size: 81 546 564 81
Fragments arranged by size:
564 546 81 81
NcoI C'CATG_G
Cuts at: 0 558 939 1082 1146 1272
Size: 558 381 143 64 126
Fragments arranged by size:
558 381 143 126 64
NspI r_CATG'y
Cuts at: 0 132 711 1053 1272
Size: 132 579 342 219
Fragments arranged by size:
579 342 219 132
PasI CC'CwG_GG
Cuts at: 0 841 1272
Size: 841 431
PflMI CCAn_nnn'nTGG
Cuts at: 0 748 895 985 1015 1180 1272
Size: 748 147 90 30 165 92
Fragments arranged by size:
748 165 147 92 90 30
PfoI T'CCnGG_A
Cuts at: 0 747 1029 1272
Size: 747 282 243
Fragments arranged by size:
747 282 243
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 83 115 586 618 1272
Size: 83 32 471 32 654
Fragments arranged by size:
654 471 83 32 32
PpuMI rG'GwC_Cy
Cuts at: 0 402 1096 1272
Size: 402 694 176
Fragments arranged by size:
694 402 176
PspXI vC'TCGA_Gb
Cuts at: 0 1241 1272
Size: 1241 31
PstI C_TGCA'G
Cuts at: 0 813 1272
Size: 813 459
PvuII CAG'CTG
Cuts at: 0 627 1272
Size: 627 645
SanDI GG'GwC_CC
Cuts at: 0 402 1272
Size: 402 870
SfcI C'TryA_G
Cuts at: 0 809 1272
Size: 809 463
SmlI C'TyrA_G
Cuts at: 0 183 1241 1272
Size: 183 1058 31
Fragments arranged by size:
1058 183 31
SphI G_CATG'C
Cuts at: 0 711 1053 1272
Size: 711 342 219
Fragments arranged by size:
711 342 219
StyI C'CwwG_G
Cuts at: 0 159 545 558 939 1082 1146 1272
Size: 159 386 13 381 143 64 126
Fragments arranged by size:
386 381 159 143 126 64 13
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 139 896 1106 1224 1272
Size: 139 757 210 118 48
Fragments arranged by size:
757 210 139 118 48
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1146 1178 1272
Size: 1146 32 94
Fragments arranged by size:
1146 94 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 45 946 1153 1272
Size: 45 901 207 119
Fragments arranged by size:
901 207 119 45
XhoI C'TCGA_G
Cuts at: 0 1241 1272
Size: 1241 31
XmnI GAAnn'nnTTC
Cuts at: 0 95 578 1272
Size: 95 483 694
Fragments arranged by size:
694 483 95
Enzymes that do cut:
AhdI AloI AlwNI ApoI AseI AvaI BanII BciVI
BclI BglI BlpI BmrI BpmI Bpu10I BpuEI BsaAI
BsaXI BseRI BseYI BsmI Bsp1286I BspHI BsrBI BstAPI
BstEII BstXI BstYI BtgI DraIII DrdI EaeI Eco57MI
EcoNI EcoO109I EcoRI HaeII Hin4I HincII MfeI MscI
MslI MspA1I NcoI NspI PasI PflMI PfoI PpiI
PpuMI PspXI PstI PvuII SanDI SfcI SmlI SphI
StyI TaqII TstI XcmI XhoI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AleI AlfI ApaI ApaLI AscI AsiSI
AvrII BaeI BamHI BanI BbeI BbsI BbvCI BcgI
BfrBI BglII Bme1580I BmgBI BmtI BplI BsaI BsaBI
BsaHI BsaWI BsgI BsiEI BsiHKAI BsiWI BsmBI BspEI
BspMI BsrDI BsrFI BsrGI BssHII BssSI BstBI BstZ17I
Bsu36I BtgZI BtsI ClaI CspCI DraI EagI EarI
EciI EcoICRI EcoRV FalI FseI FspI FspAI HindIII
HpaI KasI KpnI MluI MmeI NaeI NarI NdeI
NgoMIV NheI NotI NruI NsiI PacI PciI PmeI
PmlI PshAI PsiI PspOMI PsrI PvuI RsrII SacI
SacII SalI SapI SbfI ScaI SexAI SfiI SfoI
SgfI SgrAI SmaI SnaBI SpeI SrfI SspI StuI
SwaI TatI Tth111I XbaI XmaI ZraI