(Circular) (Six-base) MAPSORT of: pFN21AE1305.seq Check: 9133 from: 1 to: 8785 pFN21AE1305 8785 bp With 182 enzymes: SgfI * August 16, 2010 14:50 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 6595 278 Size: 53 83 186 936 5059 2468 Fragments arranged by size: 5059 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 5426 6128 1937 Size: 3489 702 4594 Fragments arranged by size: 4594 3489 702 Acc65I G'GTAC_C Cuts at: 6106 6106 Size: 8785 AclI AA'CG_TT Cuts at: 1563 6912 7285 1563 Size: 5349 373 3063 Fragments arranged by size: 5349 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3839 3978 4287 4520 4719 5088 5511 6066 Size: 2207 139 309 233 199 369 423 555 Cuts at: 6066 6848 8232 8500 1632 Size: 782 1384 268 1917 Fragments arranged by size: 2207 1917 1384 782 555 423 369 309 268 233 199 139 AflII C'TTAA_G Cuts at: 820 1017 3122 4664 820 Size: 197 2105 1542 4941 Fragments arranged by size: 4941 2105 1542 197 AflIII A'CryG_T Cuts at: 1203 7684 8661 1203 Size: 6481 977 1327 Fragments arranged by size: 6481 1327 977 AgeI A'CCGG_T Cuts at: 7616 7616 Size: 8785 AhdI GACnn_n'nnGTC Cuts at: 5146 7514 5146 Size: 2368 6417 AleI CACnn'nnGTG Cuts at: 1166 3060 1166 Size: 1894 6891 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 5624 1184 Size: 4440 4345 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4839 4871 4839 Size: 32 8753 AlwNI CAG_nnn'CTG Cuts at: 2765 4001 6528 8100 2765 Size: 1236 2527 1572 3450 Fragments arranged by size: 3450 2527 1572 1236 ApaI G_GGCC'C Cuts at: 2175 3376 2175 Size: 1201 7584 ApaLI G'TGCA_C Cuts at: 6842 7998 6842 Size: 1156 7629 ApoI r'AATT_y Cuts at: 1493 2795 3037 3296 4476 4944 4954 5867 5969 Size: 1302 242 259 1180 468 10 913 102 Cuts at: 5969 6094 6320 6472 8581 1493 Size: 125 226 152 2109 1697 Fragments arranged by size: 2109 1697 1302 1180 913 468 259 242 226 152 125 102 10 AseI AT'TA_AT Cuts at: 160 1032 4368 7339 160 Size: 872 3336 2971 1606 Fragments arranged by size: 3336 2971 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8785 AvaI C'yCGr_G Cuts at: 1870 1943 2117 6110 1870 Size: 73 174 3993 4545 Fragments arranged by size: 4545 3993 174 73 AvrII C'CTAG_G Cuts at: 5794 5794 Size: 8785 BamHI G'GATC_C Cuts at: 6115 6482 6115 Size: 367 8418 BanI G'GyrC_C Cuts at: 618 943 1160 1787 5537 6106 7561 618 Size: 325 217 627 3750 569 1455 1842 Fragments arranged by size: 3750 1842 1455 627 569 325 217 BanII G_rGCy'C Cuts at: 1391 2175 2601 3376 6104 1391 Size: 784 426 775 2728 4072 Fragments arranged by size: 4072 2728 784 775 426 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 4060 4398 5312 5838 928 Size: 971 2161 338 914 526 3875 Fragments arranged by size: 3875 2161 971 914 526 338 BbvCI CC'TCA_GC Cuts at: 2965 2965 Size: 8785 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3161 3195 6976 7010 1903 Size: 34 1224 34 3781 34 3678 Fragments arranged by size: 3781 3678 1224 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4735 6677 7887 4735 Size: 1942 1210 5633 Fragments arranged by size: 5633 1942 1210 BclI T'GATC_A Cuts at: 1548 3163 4093 1548 Size: 1615 930 6240 Fragments arranged by size: 6240 1615 930 BfrBI ATG'CAT Cuts at: 3182 3893 5611 5811 3182 Size: 711 1718 200 6156 Fragments arranged by size: 6156 1718 711 200 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2069 2120 7396 Size: 107 122 71 631 605 397 51 5276 Cuts at: 7396 136 Size: 1525 Fragments arranged by size: 5276 1525 631 605 397 122 107 71 51 BglII A'GATC_T Cuts at: 3540 4166 4453 8780 3540 Size: 626 287 4327 3545 Fragments arranged by size: 4327 3545 626 287 BlpI GC'TnA_GC Cuts at: 6202 6202 Size: 8785 Bme1580I G_kGCm'C Cuts at: 1790 2175 3376 4345 6846 8002 1790 Size: 385 1201 969 2501 1156 2573 Fragments arranged by size: 2573 2501 1201 1156 969 385 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 8785 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3353 3379 3707 Size: 416 53 83 399 24 1936 26 328 Cuts at: 3707 3770 3874 5228 6516 7474 442 Size: 63 104 1354 1288 958 1753 Fragments arranged by size: 1936 1753 1354 1288 958 416 399 328 104 83 63 53 26 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 8785 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2173 3453 7445 1239 Size: 934 1280 3992 2579 Fragments arranged by size: 3992 2579 1280 934 Bpu10I CC'TnA_GC Cuts at: 2965 2965 Size: 8785 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3938 4478 6910 7775 8073 3938 Size: 540 2432 865 298 4650 Fragments arranged by size: 4650 2432 865 540 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4497 7448 882 Size: 617 2998 2951 2219 Fragments arranged by size: 2998 2951 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2647 6576 493 Size: 711 1443 3929 2702 Fragments arranged by size: 3929 2702 1443 711 BsaBI GATnn'nnATC Cuts at: 8779 8779 Size: 8785 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 6592 6974 7609 275 Size: 53 83 186 936 5059 382 635 1451 Fragments arranged by size: 5059 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 7218 7616 7890 8037 8448 7218 Size: 398 274 147 411 7555 Fragments arranged by size: 7555 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2274 2304 3451 3481 4403 Size: 30 165 30 871 30 1147 30 922 Cuts at: 4403 4433 4839 4869 1178 Size: 30 406 30 5094 Fragments arranged by size: 5094 1147 922 871 406 165 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3169 3547 5378 1186 Size: 1983 378 1831 4593 Fragments arranged by size: 4593 1983 1831 378 BseYI C'CCAG_C Cuts at: 2162 2427 5837 7988 8484 2162 Size: 265 3410 2151 496 2463 Fragments arranged by size: 3410 2463 2151 496 265 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2559 3446 5518 1163 Size: 573 823 887 2072 4430 Fragments arranged by size: 4430 2072 887 823 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2101 6235 6996 8024 1540 Size: 275 186 100 4134 761 1028 2301 Fragments arranged by size: 4134 2301 1028 761 275 186 100 BsiHKAI G_wGCw'C Cuts at: 2021 3150 6846 6931 8002 2021 Size: 1129 3696 85 1071 2804 Fragments arranged by size: 3696 2804 1129 1071 85 BsmI GAATG_Cn' Cuts at: 6296 6389 6296 Size: 93 8692 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2021 2175 2601 3150 3376 4345 6104 Size: 399 231 154 426 549 226 969 1759 Cuts at: 6104 6846 6931 8002 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1759 1071 969 742 549 426 399 231 226 154 85 BspEI T'CCGG_A Cuts at: 8448 8448 Size: 8785 BspHI T'CATG_A Cuts at: 3103 3357 6674 8404 3103 Size: 254 3317 1730 3484 Fragments arranged by size: 3484 3317 1730 254 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4660 6140 844 Size: 3816 1480 3489 Fragments arranged by size: 3816 3489 1480 BsrBI CCG'CTC Cuts at: 1394 1823 6672 1394 Size: 429 4849 3507 Fragments arranged by size: 4849 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2535 4337 7280 7454 65 Size: 1183 1287 1802 2943 174 1396 Fragments arranged by size: 2943 1802 1396 1287 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 7429 7616 1700 Size: 110 5619 187 2869 Fragments arranged by size: 5619 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2025 2395 96 Size: 1929 370 6486 Fragments arranged by size: 6486 1929 370 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 8785 BssSI C'ACGA_G Cuts at: 3447 3663 6845 7857 3447 Size: 216 3182 1012 4375 Fragments arranged by size: 4375 3182 1012 216 BstAPI GCAn_nnn'nTGC Cuts at: 794 5918 794 Size: 5124 3661 BstBI TT'CG_AA Cuts at: 7589 7589 Size: 8785 BstEII G'GTnAC_C Cuts at: 1184 2309 1184 Size: 1125 7660 BstXI CCAn_nnnn'nTGG Cuts at: 2182 3062 2182 Size: 880 7905 BstYI r'GATC_y Cuts at: 1972 2714 3540 4166 4453 6115 6482 6867 6884 Size: 742 826 626 287 1662 367 385 17 Cuts at: 6884 8336 8422 8434 8780 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1662 1452 826 742 626 385 367 346 287 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3201 3201 Size: 8785 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 8233 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 8046 Fragments arranged by size: 8046 683 56 BtsI GCAGTG_nn' Cuts at: 2833 2848 4221 5175 6301 7095 7115 2833 Size: 15 1373 954 1126 794 20 4503 Fragments arranged by size: 4503 1373 1126 954 794 20 15 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8785 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 8750 DraI TTT'AAA Cuts at: 2448 6089 2448 Size: 3641 5144 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3997 7792 809 Size: 507 2681 3795 1802 Fragments arranged by size: 3795 2681 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2076 2098 3403 Size: 54 1425 50 275 18 246 22 1305 Cuts at: 3403 3655 6232 7121 8750 8 Size: 252 2577 889 1629 43 Fragments arranged by size: 2577 1629 1425 1305 889 275 252 246 54 50 43 22 18 EagI C'GGCC_G Cuts at: 1537 1812 2098 6232 1537 Size: 275 286 4134 4090 Fragments arranged by size: 4134 4090 286 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2607 2616 2682 2900 3133 3858 4496 5355 Size: 1254 9 66 218 233 725 638 859 Cuts at: 5355 5647 6715 1353 Size: 292 1068 3423 Fragments arranged by size: 3423 1254 1068 859 725 638 292 233 218 66 9 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 7372 7746 7892 387 Size: 1064 138 5783 374 146 1280 Fragments arranged by size: 5783 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2173 3453 3839 3978 4287 4520 4719 Size: 393 541 1280 386 139 309 233 199 Cuts at: 4719 5088 5511 6066 6848 7445 8232 8500 1239 Size: 369 423 555 782 597 787 268 1524 Fragments arranged by size: 1524 1280 787 782 597 555 541 423 393 386 369 309 268 233 199 139 EcoNI CCTnn'n_nnAGG Cuts at: 2077 2133 4667 2077 Size: 56 2534 6195 Fragments arranged by size: 6195 2534 56 EcoO109I rG'GnC_Cy Cuts at: 2082 2121 2178 2082 Size: 39 57 8689 Fragments arranged by size: 8689 57 39 EcoRI G'AATT_C Cuts at: 6094 6472 6094 Size: 378 8407 FspI TGC'GCA Cuts at: 7291 7291 Size: 8785 HaeII r_GCGC'y Cuts at: 1678 2054 2249 7932 1678 Size: 376 195 5683 2531 Fragments arranged by size: 5683 2531 376 195 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2585 2617 3148 3180 3241 Size: 32 135 32 1013 32 531 32 61 Cuts at: 3241 3273 3451 3483 4091 4123 4403 4435 4678 Size: 32 178 32 608 32 280 32 243 Cuts at: 4678 4710 4979 5011 5132 5164 7426 7458 7500 Size: 32 269 32 121 32 2262 32 42 Cuts at: 7500 7532 1373 Size: 32 2626 Fragments arranged by size: 2626 2262 1013 608 531 280 269 243 178 135 121 61 42 32 32 32 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4017 6033 6129 6375 669 Size: 988 281 2079 2016 96 246 3079 Fragments arranged by size: 3079 2079 2016 988 281 246 96 HindIII A'AGCT_T Cuts at: 748 4445 748 Size: 3697 5088 HpaI GTT'AAC Cuts at: 6375 6375 Size: 8785 KpnI G_GTAC'C Cuts at: 6110 6110 Size: 8785 MfeI C'AATT_G Cuts at: 5511 6384 5511 Size: 873 7912 MluI A'CGCG_T Cuts at: 8661 8661 Size: 8785 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5154 7899 8083 1305 Size: 3849 2745 184 2007 Fragments arranged by size: 3849 2745 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3657 10 Size: 54 1425 343 1825 5138 Fragments arranged by size: 5138 1825 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3060 3318 3663 3959 4403 5606 6743 Size: 648 1894 258 345 296 444 1203 1137 Cuts at: 6743 7102 7261 518 Size: 359 159 2042 Fragments arranged by size: 2042 1894 1203 1137 648 444 359 345 296 258 159 MspA1I CmG'CkG Cuts at: 1606 2111 4939 6201 6878 8026 8271 1606 Size: 505 2828 1262 677 1148 245 2120 Fragments arranged by size: 2828 2120 1262 1148 677 505 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 8233 NdeI CA'TA_TG Cuts at: 387 3670 5802 387 Size: 3283 2132 3370 Fragments arranged by size: 3370 3283 2132 NheI G'CTAG_C Cuts at: 1052 1052 Size: 8785 NotI GC'GGCC_GC Cuts at: 6232 6232 Size: 8785 NruI TCG'CGA Cuts at: 2011 2011 Size: 8785 NsiI A_TGCA'T Cuts at: 3184 3895 5613 5813 3184 Size: 711 1718 200 6156 Fragments arranged by size: 6156 1718 711 200 NspI r_CATG'y Cuts at: 1132 1229 5104 5615 5811 6143 7688 1132 Size: 97 3875 511 196 332 1545 2229 Fragments arranged by size: 3875 2229 1545 511 332 196 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 8785 PciI A'CATG_T Cuts at: 7684 7684 Size: 8785 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 8785 PfoI T'CCnGG_A Cuts at: 4857 4857 Size: 8785 PmeI GTTT'AAAC Cuts at: 6089 6089 Size: 8785 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4839 4871 6854 6886 8367 8399 4839 Size: 32 1983 32 1481 32 5225 Fragments arranged by size: 5225 1983 1481 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 3081 4904 1613 Size: 1468 1823 5494 Fragments arranged by size: 5494 1823 1468 PsiI TTA'TAA Cuts at: 6355 6355 Size: 8785 PspOMI G'GGCC_C Cuts at: 2171 3372 2171 Size: 1201 7584 PstI C_TGCA'G Cuts at: 830 4686 6137 830 Size: 3856 1451 3478 Fragments arranged by size: 3856 3478 1451 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8785 PvuII CAG'CTG Cuts at: 4939 4939 Size: 8785 SalI G'TCGA_C Cuts at: 1936 6127 1936 Size: 4191 4594 SbfI CC_TGCA'GG Cuts at: 6137 6137 Size: 8785 ScaI AGT'ACT Cuts at: 7033 8745 7033 Size: 1712 7073 SfcI C'TryA_G Cuts at: 826 1045 3544 3607 4682 4722 4894 5154 5200 Size: 219 2499 63 1075 40 172 260 46 Cuts at: 5200 6036 6133 7268 7949 8140 826 Size: 836 97 1135 681 191 1471 Fragments arranged by size: 2499 1471 1135 1075 836 681 260 219 191 172 97 63 46 40 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8785 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8785 SmaI CCC'GGG Cuts at: 1872 2119 6112 1872 Size: 247 3993 4545 Fragments arranged by size: 4545 3993 247 SmlI C'TyrA_G Cuts at: 820 1017 1943 3122 3917 4457 4664 6889 7790 Size: 197 926 1179 795 540 207 2225 901 Cuts at: 7790 8052 820 Size: 262 1553 Fragments arranged by size: 2225 1553 1179 926 901 795 540 262 207 197 SnaBI TAC'GTA Cuts at: 493 2647 6576 493 Size: 2154 3929 2702 Fragments arranged by size: 3929 2702 2154 SpeI A'CTAG_T Cuts at: 152 152 Size: 8785 SphI G_CATG'C Cuts at: 1132 5104 5615 5811 6143 1132 Size: 3972 511 196 332 3774 Fragments arranged by size: 3972 3774 511 332 196 SspI AAT'ATT Cuts at: 5 52 3929 6709 5 Size: 47 3877 2780 2081 Fragments arranged by size: 3877 2780 2081 47 StuI AGG'CCT Cuts at: 1517 2072 2871 3160 1517 Size: 555 799 289 7142 Fragments arranged by size: 7142 799 555 289 StyI C'CwwG_G Cuts at: 513 1065 2181 2205 3558 4137 5794 6224 513 Size: 552 1116 24 1353 579 1657 430 3074 Fragments arranged by size: 3074 1657 1353 1116 579 552 430 24 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4530 6812 6829 6982 7167 7612 325 Size: 1255 2950 2282 17 153 185 445 1498 Fragments arranged by size: 2950 2282 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2025 2395 2695 Size: 274 80 33 51 1443 48 370 300 Cuts at: 2695 3221 3778 5567 7031 8743 96 Size: 526 557 1789 1464 1712 138 Fragments arranged by size: 1789 1712 1464 1443 557 526 370 300 274 138 80 51 48 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 8745 8777 8745 Size: 32 8753 Tth111I GACn'n_nGTC Cuts at: 1316 4151 1316 Size: 2835 5950 XbaI T'CTAG_A Cuts at: 4378 6121 4378 Size: 1743 7042 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2348 2424 4307 2348 Size: 76 1883 6826 Fragments arranged by size: 6826 1883 76 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 8785 XmaI C'CCGG_G Cuts at: 1870 2117 6110 1870 Size: 247 3993 4545 Fragments arranged by size: 4545 3993 247 XmnI GAAnn'nnTTC Cuts at: 4131 5940 6914 4131 Size: 1809 974 6002 Fragments arranged by size: 6002 1809 974 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 6593 276 Size: 53 83 186 936 5059 2468 Fragments arranged by size: 5059 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NruI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AscI BaeI BbeI BplI BsiWI BsmBI BstZ17I DraIII EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV PacI PmlI PpuMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI