Restriction map for
pFN21AE1305
(Linear) (Six-base) MAPSORT of: KIEE1305.seq Check: 6702 from: 1 to: 4080
KIEE1305.orf
With 182 enzymes: SgfI *
March 1, 2013 10:20 ..
AccI GT'mk_AC
Cuts at: 0 3421 4080
Size: 3421 659
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1834 1973 2282 2515 2714 3083 3506 4061
Size: 1834 139 309 233 199 369 423 555
Cuts at: 4061 4080
Size: 19
Fragments arranged by size:
1834 555 423 369 309 233 199 139
19
AflII C'TTAA_G
Cuts at: 0 1117 2659 4080
Size: 1117 1542 1421
Fragments arranged by size:
1542 1421 1117
AhdI GACnn_n'nnGTC
Cuts at: 0 3141 4080
Size: 3141 939
AleI CACnn'nnGTG
Cuts at: 0 1055 4080
Size: 1055 3025
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 3619 4080
Size: 3619 461
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2834 2866 4080
Size: 2834 32 1214
Fragments arranged by size:
2834 1214 32
AlwNI CAG_nnn'CTG
Cuts at: 0 760 1996 4080
Size: 760 1236 2084
Fragments arranged by size:
2084 1236 760
ApaI G_GGCC'C
Cuts at: 0 170 1371 4080
Size: 170 1201 2709
Fragments arranged by size:
2709 1201 170
ApoI r'AATT_y
Cuts at: 0 790 1032 1291 2471 2939 2949 3862 3964
Size: 790 242 259 1180 468 10 913 102
Cuts at: 3964 4080
Size: 116
Fragments arranged by size:
1180 913 790 468 259 242 116 102
10
AseI AT'TA_AT
Cuts at: 0 2363 4080
Size: 2363 1717
AvaI C'yCGr_G
Cuts at: 0 112 4080
Size: 112 3968
AvrII C'CTAG_G
Cuts at: 0 3789 4080
Size: 3789 291
BanI G'GyrC_C
Cuts at: 0 3532 4080
Size: 3532 548
BanII G_rGCy'C
Cuts at: 0 170 596 1371 4080
Size: 170 426 775 2709
Fragments arranged by size:
2709 775 426 170
BbsI GAAGACnn'nnnn_
Cuts at: 0 2055 2393 3307 3833 4080
Size: 2055 338 914 526 247
Fragments arranged by size:
2055 914 526 338 247
BbvCI CC'TCA_GC
Cuts at: 0 960 4080
Size: 960 3120
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1156 1190 4080
Size: 1156 34 2890
Fragments arranged by size:
2890 1156 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 2730 4080
Size: 2730 1350
BclI T'GATC_A
Cuts at: 0 1158 2088 4080
Size: 1158 930 1992
Fragments arranged by size:
1992 1158 930
BfrBI ATG'CAT
Cuts at: 0 1177 1888 3606 3806 4080
Size: 1177 711 1718 200 274
Fragments arranged by size:
1718 1177 711 274 200
BglI GCCn_nnn'nGGC
Cuts at: 0 64 115 4080
Size: 64 51 3965
Fragments arranged by size:
3965 64 51
BglII A'GATC_T
Cuts at: 0 1535 2161 2448 4080
Size: 1535 626 287 1632
Fragments arranged by size:
1632 1535 626 287
Bme1580I G_kGCm'C
Cuts at: 0 170 1371 2340 4080
Size: 170 1201 969 1740
Fragments arranged by size:
1740 1201 969 170
BmrI ACTGGGnnnn_n'
Cuts at: 0 1348 1374 1702 1765 1869 3223 4080
Size: 1348 26 328 63 104 1354 857
Fragments arranged by size:
1354 1348 857 328 104 63 26
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 168 1448 4080
Size: 168 1280 2632
Fragments arranged by size:
2632 1280 168
Bpu10I CC'TnA_GC
Cuts at: 0 960 4080
Size: 960 3120
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1933 2473 4080
Size: 1933 540 1607
Fragments arranged by size:
1933 1607 540
BsaI GGTCTCn'nnnn_
Cuts at: 0 2492 4080
Size: 2492 1588
BsaAI yAC'GTr
Cuts at: 0 642 4080
Size: 642 3438
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 269 299 1446 1476 2398 2428 2834 2864
Size: 269 30 1147 30 922 30 406 30
Cuts at: 2864 4080
Size: 1216
Fragments arranged by size:
1216 1147 922 406 269 30 30 30
30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1164 1542 3373 4080
Size: 1164 378 1831 707
Fragments arranged by size:
1831 1164 707 378
BseYI C'CCAG_C
Cuts at: 0 157 422 3832 4080
Size: 157 265 3410 248
Fragments arranged by size:
3410 265 248 157
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 554 1441 3513 4080
Size: 554 887 2072 567
Fragments arranged by size:
2072 887 567 554
BsiEI CG_ry'CG
Cuts at: 0 96 4080
Size: 96 3984
BsiHKAI G_wGCw'C
Cuts at: 0 16 1145 4080
Size: 16 1129 2935
Fragments arranged by size:
2935 1129 16
Bsp1286I G_dGCh'C
Cuts at: 0 16 170 596 1145 1371 2340 4080
Size: 16 154 426 549 226 969 1740
Fragments arranged by size:
1740 969 549 426 226 154 16
BspHI T'CATG_A
Cuts at: 0 1098 1352 4080
Size: 1098 254 2728
Fragments arranged by size:
2728 1098 254
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 2655 4080
Size: 2655 1425
BsrDI GCAATG_nn'
Cuts at: 0 530 2332 4080
Size: 530 1802 1748
Fragments arranged by size:
1802 1748 530
BsrGI T'GTAC_A
Cuts at: 0 20 390 4080
Size: 20 370 3690
Fragments arranged by size:
3690 370 20
BssSI C'ACGA_G
Cuts at: 0 1442 1658 4080
Size: 1442 216 2422
Fragments arranged by size:
2422 1442 216
BstAPI GCAn_nnn'nTGC
Cuts at: 0 3913 4080
Size: 3913 167
BstEII G'GTnAC_C
Cuts at: 0 304 4080
Size: 304 3776
BstXI CCAn_nnnn'nTGG
Cuts at: 0 177 1057 4080
Size: 177 880 3023
Fragments arranged by size:
3023 880 177
BstYI r'GATC_y
Cuts at: 0 709 1535 2161 2448 4080
Size: 709 826 626 287 1632
Fragments arranged by size:
1632 826 709 626 287
Bsu36I CC'TnA_GG
Cuts at: 0 1196 4080
Size: 1196 2884
BtsI GCAGTG_nn'
Cuts at: 0 828 843 2216 3170 4080
Size: 828 15 1373 954 910
Fragments arranged by size:
1373 954 910 828 15
DraI TTT'AAA
Cuts at: 0 443 4080
Size: 443 3637
DrdI GACnn_nn'nnGTC
Cuts at: 0 1992 4080
Size: 1992 2088
EaeI y'GGCC_r
Cuts at: 0 71 93 1398 1650 4080
Size: 71 22 1305 252 2430
Fragments arranged by size:
2430 1305 252 71 22
EagI C'GGCC_G
Cuts at: 0 93 4080
Size: 93 3987
EarI CTCTTCn'nnn_
Cuts at: 0 602 611 677 895 1128 1853 2491 3350
Size: 602 9 66 218 233 725 638 859
Cuts at: 3350 3642 4080
Size: 292 438
Fragments arranged by size:
859 725 638 602 438 292 233 218
66 9
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 168 1448 1834 1973 2282 2515 2714 3083
Size: 168 1280 386 139 309 233 199 369
Cuts at: 3083 3506 4061 4080
Size: 423 555 19
Fragments arranged by size:
1280 555 423 386 369 309 233 199
168 139 19
EcoNI CCTnn'n_nnAGG
Cuts at: 0 72 128 2662 4080
Size: 72 56 2534 1418
Fragments arranged by size:
2534 1418 72 56
EcoO109I rG'GnC_Cy
Cuts at: 0 77 116 173 4080
Size: 77 39 57 3907
Fragments arranged by size:
3907 77 57 39
HaeII r_GCGC'y
Cuts at: 0 49 244 4080
Size: 49 195 3836
Fragments arranged by size:
3836 195 49
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 580 612 1143 1175 1236 1268 1446 1478
Size: 580 32 531 32 61 32 178 32
Cuts at: 1478 2086 2118 2398 2430 2673 2705 2974 3006
Size: 608 32 280 32 243 32 269 32
Cuts at: 3006 3127 3159 4080
Size: 121 32 921
Fragments arranged by size:
921 608 580 531 280 269 243 178
121 61 32 32 32 32 32 32
32 32 32
HincII GTy'rAC
Cuts at: 0 2012 4028 4080
Size: 2012 2016 52
Fragments arranged by size:
2016 2012 52
HindIII A'AGCT_T
Cuts at: 0 2440 4080
Size: 2440 1640
MfeI C'AATT_G
Cuts at: 0 3506 4080
Size: 3506 574
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 3149 4080
Size: 3149 931
MscI TGG'CCA
Cuts at: 0 1652 4080
Size: 1652 2428
MslI CAynn'nnrTG
Cuts at: 0 1055 1313 1658 1954 2398 3601 4080
Size: 1055 258 345 296 444 1203 479
Fragments arranged by size:
1203 1055 479 444 345 296 258
MspA1I CmG'CkG
Cuts at: 0 106 2934 4080
Size: 106 2828 1146
Fragments arranged by size:
2828 1146 106
NdeI CA'TA_TG
Cuts at: 0 1665 3797 4080
Size: 1665 2132 283
Fragments arranged by size:
2132 1665 283
NruI TCG'CGA
Cuts at: 0 6 4080
Size: 6 4074
NsiI A_TGCA'T
Cuts at: 0 1179 1890 3608 3808 4080
Size: 1179 711 1718 200 272
Fragments arranged by size:
1718 1179 711 272 200
NspI r_CATG'y
Cuts at: 0 3099 3610 3806 4080
Size: 3099 511 196 274
Fragments arranged by size:
3099 511 274 196
PfoI T'CCnGG_A
Cuts at: 0 2852 4080
Size: 2852 1228
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2834 2866 4080
Size: 2834 32 1214
Fragments arranged by size:
2834 1214 32
PshAI GACnn'nnGTC
Cuts at: 0 1076 2899 4080
Size: 1076 1823 1181
Fragments arranged by size:
1823 1181 1076
PspOMI G'GGCC_C
Cuts at: 0 166 1367 4080
Size: 166 1201 2713
Fragments arranged by size:
2713 1201 166
PstI C_TGCA'G
Cuts at: 0 2681 4080
Size: 2681 1399
PvuII CAG'CTG
Cuts at: 0 2934 4080
Size: 2934 1146
SfcI C'TryA_G
Cuts at: 0 1539 1602 2677 2717 2889 3149 3195 4031
Size: 1539 63 1075 40 172 260 46 836
Cuts at: 4031 4080
Size: 49
Fragments arranged by size:
1539 1075 836 260 172 63 49 46
40
SmaI CCC'GGG
Cuts at: 0 114 4080
Size: 114 3966
SmlI C'TyrA_G
Cuts at: 0 1117 1912 2452 2659 4080
Size: 1117 795 540 207 1421
Fragments arranged by size:
1421 1117 795 540 207
SnaBI TAC'GTA
Cuts at: 0 642 4080
Size: 642 3438
SphI G_CATG'C
Cuts at: 0 3099 3610 3806 4080
Size: 3099 511 196 274
Fragments arranged by size:
3099 511 274 196
SspI AAT'ATT
Cuts at: 0 1924 4080
Size: 1924 2156
StuI AGG'CCT
Cuts at: 0 67 866 1155 4080
Size: 67 799 289 2925
Fragments arranged by size:
2925 799 289 67
StyI C'CwwG_G
Cuts at: 0 176 200 1553 2132 3789 4080
Size: 176 24 1353 579 1657 291
Fragments arranged by size:
1657 1353 579 291 176 24
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 2525 4080
Size: 2525 1555
TatI w'GTAC_w
Cuts at: 0 20 390 690 1216 1773 3562 4080
Size: 20 370 300 526 557 1789 518
Fragments arranged by size:
1789 557 526 518 370 300 20
Tth111I GACn'n_nGTC
Cuts at: 0 2146 4080
Size: 2146 1934
XbaI T'CTAG_A
Cuts at: 0 2373 4080
Size: 2373 1707
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 343 419 2302 4080
Size: 343 76 1883 1778
Fragments arranged by size:
1883 1778 343 76
XmaI C'CCGG_G
Cuts at: 0 112 4080
Size: 112 3968
XmnI GAAnn'nnTTC
Cuts at: 0 2126 3935 4080
Size: 2126 1809 145
Fragments arranged by size:
2126 1809 145
Enzymes that do cut:
AccI AcuI AflII AhdI AleI AlfI AloI AlwNI
ApaI ApoI AseI AvaI AvrII BanI BanII BbsI
BbvCI BcgI BciVI BclI BfrBI BglI BglII Bme1580I
BmrI BpmI Bpu10I BpuEI BsaI BsaAI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI Bsp1286I BspHI BspMI BsrDI
BsrGI BssSI BstAPI BstEII BstXI BstYI Bsu36I BtsI
DraI DrdI EaeI EagI EarI Eco57MI EcoNI EcoO109I
HaeII Hin4I HincII HindIII MfeI MmeI MscI MslI
MspA1I NdeI NruI NsiI NspI PfoI PpiI PshAI
PspOMI PstI PvuII SfcI SmaI SmlI SnaBI SphI
SspI StuI StyI TaqII TatI Tth111I XbaI XcmI
XmaI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflIII AgeI ApaLI
AscI AsiSI BaeI BamHI BbeI BlpI BmgBI BmtI
BplI BsaBI BsaHI BsaWI BsiWI BsmI BsmBI BspEI
BsrBI BsrFI BssHII BstBI BstZ17I BtgI BtgZI ClaI
CspCI DraIII EciI EcoICRI EcoRI EcoRV FalI FseI
FspI FspAI HpaI KasI KpnI MluI NaeI NarI
NcoI NgoMIV NheI NotI PacI PasI PciI PflMI
PmeI PmlI PpuMI PsiI PspXI PsrI PvuI RsrII
SacI SacII SalI SanDI SapI SbfI ScaI SexAI
SfiI SfoI SgfI SgrAI SpeI SrfI SwaI TstI
XhoI ZraI