################################## # NO CUT ENZYMES ( 153 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AcyI AflII AflIII AgeI AhdI Alw44I AlwI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AvaI AvaII AviII AvrII BalI BamHI BbeI BbrPI BbvCI BcgI BciVI BclI BfrI BglI BlnI BlpI Bpu1102I BsaAI BsaBI BsaHI BsaI BsaWI BseAI BseRI BsgI BsiEI BsiWI BsmBI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstZ17I Bsu36I CelII ClaI CpoI DraIII DrdI DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV FbaI FseI FspI HgaI Hin1I HpaI HphI KasI KpnI KspI MamI MluI MluNI MroI MscI MslI MvnI NarI NcoI NdeI NheI NotI NruI NspI NspV PacI PaeR7I PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI TaqI Tsp45I TthHB8I Van91I XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 48 enzymes) # ################################### AccI 1017 GTCTAC GT^[AC][GT]AC AcsI 682 AAATTT [GA]^AATT[CT] AlwNI 16 CAGGCCCTG CAG[ACGT][ACGT][ACGT]^CTG ApoI 682 AAATTT [GA]^AATT[CT] AspI 265 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC BanI 908 GGCACC G^G[CT][GA]CC BanII 386 GAGCCC G[GA]GC[CT]^C BbsI 188 GAAGAC GAAGAC(2/6) BcnI 802 CCCGG CC^[GC]GG BglII 48 AGATCT A^GATCT BpuAI 188 GAAGAC GAAGAC(2/6) BsmAI 42 GTCTC GTCTC(1/5) BsmFI 576 GGGAC GGGAC(10/14) BsmI 624 GCATTC GAATGC(1/-1) BsrFI 870 GCCGGC [GA]^CCGG[CT] BstYI 48 AGATCT [GA]^GATC[CT] Cfr10I 870 GCCGGC [GA]^CCGG[CT] DpnII 49 GATC ^GATC DraI 679 TTTAAA TTT^AAA DraII 17 AGGCCCT [GA]G^G[ACGT]CC[CT] EcoO109I 17 AGGCCCT [GA]G^G[ACGT]CC[CT] EcoT22I 622 ATGCAT ATGCA^T HaeII 125 AGCGCC [GA]GCGC^[CT] HincII 445 GTCAAC GT[CT]^[GA]AC HindII 445 GTCAAC GT[CT]^[GA]AC HindIII 667 AAGCTT A^AGCTT MboI 49 GATC ^GATC MfeI 301 CAATTG C^AATTG MflI 48 AGATCT [GA]^GATC[CT] MunI 301 CAATTG C^AATTG NaeI 870 GCCGGC GCC^GGC NciI 802 CCCGG CC^[GC]GG NdeII 49 GATC ^GATC NgoMIV 870 GCCGGC G^CCGGC NlaIV 908 GGCACC GG[ACGT]^[ACGT]CC NsiI 622 ATGCAT ATGCA^T PflFI 265 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC PleI 12 GAGTC GAGTC(4/5) PstI 148 CTGCAG CTGCA^G PvuII 62 CAGCTG CAG^CTG SapI 939 GAAGAGC GCTCTTC(1/4) Sau3AI 49 GATC ^GATC SmlI 44 CTCAAG C^T[CT][GA]AG StuI 980 AGGCCT AGG^CCT TfiI 611 GATTC G^A[AT]TC Tth111I 265 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC XbaI 513 TCTAGA T^CTAGA XhoII 48 AGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### AfaI 90 GTAC GT^AC 102 GTAC AspHI 931 GAGCAC G[AT]GC[AT]^C 1009 GAGCAC BbvI 64 GCTGC GCAGC(8/12) 828 GCTGC BfaI 26 CTAG C^TAG 514 CTAG BpmI 402 CTCCAG CTGGAG(16/14) 916 CTCCAG BsiHKAI 931 GAGCAC G[AT]GC[AT]^C 1009 GAGCAC BstXI 158 CCAAGCGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 185 CCAGAAGACTGG CfoI 126 GCGC GCG^C 831 GCGC Cfr13I 18 GGCCC G^G[ACGT]CC 877 GGCCC DdeI 314 CTGAG C^T[ACGT]AG 986 CTGAG HhaI 126 GCGC GCG^C 831 GCGC HinP1I 126 GCGC G^CGC 831 GCGC HinfI 12 GAGTC G^A[ACGT]TC 611 GATTC MaeI 26 CTAG C^TAG 514 CTAG MaeII 329 ACGT A^CGT 551 ACGT MspA1I 62 CAGCTG C[AC]G^C[GT]G 880 CCGCTG RsaI 90 GTAC GT^AC 102 GTAC Sau96I 18 GGCCC G^G[ACGT]CC 877 GGCCC SfcI 148 CTGCAG C^T[GA][CT]AG 795 CTACAG TseI 64 GCTGC G^C[AT]GC 828 GCTGC TspRI 267 CACTG CA[GC]TG[ACGT][ACGT]^ 417 CAGTG ######################################### # 3 OR MORE CUT ENZYMES ( 36 enzymes) # ######################################### AciI AluI BmyI BsaJI BsiYI BslI Bsp1286I BsrI BssKI BstF5I BstNI Cac8I EarI EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HpaII ItaI Ksp632I MaeIII MboII MnlI MseI MspI MvaI MwoI NlaIII ScrFI SfaNI StyI Tru9I Tsp509I