################################## # NO CUT ENZYMES ( 162 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AcyI AflII AflIII AgeI AhdI Alw44I AocI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AviII AvrII BalI BamHI BanI BbeI BbrPI BbvCI BcgI BciVI BcnI BfrI BglI BlnI BlpI Bpu1102I BsaAI BsaBI BsaHI BsaI BsaWI BseAI BsgI BsiEI BsmBI BsmI BsoBI BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrFI BsrI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraI DraII DraIII DrdI DsaI EaeI EagI Eam1105I EclXI Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoT14I FauI FseI FspI HapII Hin1I HincII HindII HindIII HpaI HpaII KasI KpnI KspI MamI MfeI MluI MluNI MroI MscI MspI MunI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NspI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI PstI PvuI RcaI RsrII SacI SacII SalI SapI SbfI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspI StuI StyI SwaI Tth111I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 35 enzymes) # ################################### AclI 682 AACGTT AA^CGTT AlwNI 664 CAGTGCCTG CAG[ACGT][ACGT][ACGT]^CTG Aor51HI 750 AGCGCT AGC^GCT AspHI 22 GTGCTC G[AT]GC[AT]^C BanII 654 GGGCTC G[GA]GC[CT]^C BbvI 1021 GCTGC GCAGC(8/12) BclI 660 TGATCA T^GATCA BfaI 748 CTAG C^TAG BglII 255 AGATCT A^GATCT BpmI 224 CTGGAG CTGGAG(16/14) BseRI 986 CTCCTC GAGGAG(10/8) BsiHKAI 22 GTGCTC G[AT]GC[AT]^C BsiWI 859 CGTACG C^GTACG Bsp1407I 634 TGTACA T^GTACA BspMI 1008 ACCTGC ACCTGC(4/8) BsrGI 634 TGTACA T^GTACA Cac8I 741 GCTTGC GC[ACGT]^[ACGT]GC Eco47III 750 AGCGCT AGC^GCT EcoT22I 305 ATGCAT ATGCA^T FbaI 660 TGATCA T^GATCA Fnu4HI 1021 GCTGC GC^[ACGT]GC HaeIII 265 GGCC GG^CC HgaI 675 GCGTC GACGC(5/10) ItaI 1021 GCTGC GC^[ACGT]GC MaeI 748 CTAG C^TAG MslI 350 CATATCAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NsiI 305 ATGCAT ATGCA^T PflMI 554 CCACTTCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Psp1406I 682 AACGTT AA^CGTT ScaI 614 AGTACT AGT^ACT SfcI 1088 CTATAG C^T[GA][CT]AG SspBI 634 TGTACA T^GTACA TseI 1021 GCTGC G^C[AT]GC Tsp45I 467 GTGAC ^GT[GC]AC Van91I 554 CCACTTCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 29 enzymes) # ################################### AciI 154 CCGC CCGC(-3/-1) 32 GCGG AlwI 201 GGATC GGATC(4/5) 448 GGATC AvaII 151 GGACC G^G[AT]CC 908 GGTCC BbsI 142 GAAGAC GAAGAC(2/6) 970 GAAGAC BmyI 22 GTGCTC G[AGT]GC[ACT]^C 654 GGGCTC BpuAI 142 GAAGAC GAAGAC(2/6) 970 GAAGAC BsiYI 490 CCAAAAGAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 554 CCACTTCATGG BslI 490 CCAAAAGAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 554 CCACTTCATGG BsmAI 227 GAGAC GTCTC(1/5) 915 GTCTC Bsp1286I 22 GTGCTC G[AGT]GC[ACT]^C 654 GGGCTC BstF5I 899 CATCC GGATG(2/0) 1080 GGATG BstYI 255 AGATCT [GA]^GATC[CT] 448 GGATCT Cfr13I 151 GGACC G^G[ACGT]CC 908 GGTCC EarI 606 CTCTTC CTCTTC(1/4) 76 GAAGAG EcoRV 174 GATATC GAT^ATC 826 GATATC FokI 899 CATCC GGATG(9/13) 1080 GGATG HaeII 3 GGCGCT [GA]GCGC^[CT] 750 AGCGCT HphI 301 GGTGA GGTGA(8/7) 178 TCACC Ksp632I 606 CTCTTC CTCTTC(1/4) 76 GAAGAG MflI 255 AGATCT [GA]^GATC[CT] 448 GGATCT MseI 416 TTAA T^TAA 473 TTAA MwoI 878 GCTACATCGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 1012 GCTAAGGTAGC PvuII 182 CAGCTG CAG^CTG 630 CAGCTG Sau96I 151 GGACC G^G[ACGT]CC 908 GGTCC SfaNI 304 GATGC GCATC(5/9) 307 GCATC TaqI 164 TCGA T^CGA 1038 TCGA Tru9I 416 TTAA T^TAA 473 TTAA TthHB8I 164 TCGA T^CGA 1038 TCGA XhoII 255 AGATCT [GA]^GATC[CT] 448 GGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 32 enzymes) # ######################################### AcsI AfaI AluI ApoI BsaJI BsmFI BssKI BstNI CfoI DdeI DpnII EcoRII HhaI HinP1I HinfI MaeII MaeIII MboI MboII MnlI MspA1I MvaI NdeII NlaIII NlaIV PleI RsaI Sau3AI ScrFI TfiI Tsp509I TspRI