################################## # NO CUT ENZYMES ( 137 enzymes) # ################################## Acc65I AccI AccIII AclI AflII AflIII AgeI AhdI Alw44I AlwNI AocI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AviII AvrII BalI BamHI BbrPI BbsI BbvCI BciVI BclI BfrI BglI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaI BsaWI BseAI BseRI BsiEI BsiWI BsmBI BsmFI BspEI BspHI BspLU11I BsrBI BsrFI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstXI BstZ17I Bsu36I CelII Cfr10I CpoI DraI DraII EaeI EagI Eam1105I EclXI Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRV EcoT22I FbaI FseI FspI HincII HindII HindIII HpaI KpnI KspI MfeI MluI MluNI MroI MscI MseI MspA1I MunI NaeI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI PvuII RcaI RsrII SacII SalI SbfI SfiI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspI StuI SwaI Tru9I Van91I XbaI XcmI XhoI XmnI ################################### # ONE CUT ENZYMES ( 45 enzymes) # ################################### AatII 30 GACGTC GACGT^C Aor51HI 479 AGCGCT AGC^GCT AspI 973 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 44 CCCGGG C^[CT]CG[GA]G BanI 61 GGCGCC G^G[CT][GA]CC BanII 959 GAGCTC G[GA]GC[CT]^C BbeI 61 GGCGCC GGCGC^C BcgI 855 GCAGGACAATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BfaI 241 CTAG C^TAG BpmI 508 CTGGAG CTGGAG(16/14) BsaBI 612 GATTTACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsgI 94 GTGCAG GTGCAG(16/14) BsmAI 1043 GAGAC GTCTC(1/5) BsoBI 44 CCCGGG C^[CT]CG[GA]G BspDI 619 ATCGAT AT^CGAT BsrDI 368 GCAATG GCAATG(2/0) BstF5I 534 CATCC GGATG(2/0) ClaI 619 ATCGAT AT^CGAT DraIII 604 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 30 GACGTCCGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC DsaI 844 CCGTGG C^C[GA][CT]GG EarI 570 GAAGAG CTCTTC(1/4) Eco47III 479 AGCGCT AGC^GCT EcoRI 80 GAATTC G^AATTC EcoT14I 437 CCTTGG C^C[AT][AT]GG FokI 534 CATCC GGATG(9/13) KasI 61 GGCGCC G^GCGCC Ksp632I 570 GAAGAG CTCTTC(1/4) MaeI 241 CTAG C^TAG MaeII 31 ACGT A^CGT MamI 612 GATTTACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC NarI 61 GGCGCC GG^CGCC NlaIII 673 CATG CATG^ PflFI 973 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC SacI 959 GAGCTC GAGCT^C SapI 570 GAAGAGC GCTCTTC(1/4) ScaI 425 AGTACT AGT^ACT SexAI 605 ACCTGGT A^CC[AT]GGT SfaNI 433 GATGC GCATC(5/9) SfoI 61 GGCGCC GGC^GCC SmaI 44 CCCGGG CCC^GGG StyI 437 CCTTGG C^C[AT][AT]GG Tth111I 973 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC XmaCI 44 CCCGGG C^CCGGG XmaI 44 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 29 enzymes) # ################################### AccII 36 CGCG CG^CG 70 CGCG AcsI 80 GAATTC [GA]^AATT[CT] 346 AAATTT AcyI 30 GACGTC G[GA]^CG[CT]C 61 GGCGCC ApoI 80 GAATTC [GA]^AATT[CT] 346 AAATTT AspHI 442 GAGCAC G[AT]GC[AT]^C 959 GAGCTC AvaII 552 GGACC G^G[AT]CC 639 GGTCC BsaHI 30 GACGTC G[GA]^CG[CT]C 61 GGCGCC BsiHKAI 442 GAGCAC G[AT]GC[AT]^C 959 GAGCTC BsmI 714 GAATGC GAATGC(1/-1) 926 GCATTC Bsp1407I 404 TGTACA T^GTACA 518 TGTACA BspMI 593 ACCTGC ACCTGC(4/8) 878 ACCTGC BsrGI 404 TGTACA T^GTACA 518 TGTACA BsrI 202 ACTGG ACTGG(1/-1) 888 ACTGG BstUI 36 CGCG CG^CG 70 CGCG BstYI 666 AGATCC [GA]^GATC[CT] 1052 GGATCT DdeI 429 CTCAG C^T[ACGT]AG 740 CTGAG FauI 58 GCGGG CCCGC(4/6) 265 GCGGG HaeII 61 GGCGCC [GA]GCGC^[CT] 479 AGCGCT HgaI 37 GCGTC GACGC(5/10) 575 GCGTC Hin1I 30 GACGTC G[GA]^CG[CT]C 61 GGCGCC MflI 666 AGATCC [GA]^GATC[CT] 1052 GGATCT MvnI 36 CGCG CG^CG 70 CGCG NlaIV 11 GGCTCC GG[ACGT]^[ACGT]CC 61 GGCGCC PstI 391 CTGCAG CTGCA^G 421 CTGCAG SmlI 788 CTCAAG C^T[CT][GA]AG 850 CTCAAG SspBI 404 TGTACA T^GTACA 518 TGTACA TaqI 620 TCGA T^CGA 833 TCGA TthHB8I 620 TCGA T^CGA 833 TCGA XhoII 666 AGATCC [GA]^GATC[CT] 1052 GGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 47 enzymes) # ######################################### AciI AfaI AluI AlwI BbvI BcnI BmyI BsaJI BsiYI BslI Bsp1286I BssKI BstNI Cac8I CfoI Cfr13I DpnII EcoRII Fnu4HI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI MaeIII MboI MboII MnlI MslI MspI MvaI MwoI NciI NdeII RsaI Sau3AI Sau96I ScrFI SfcI TfiI TseI Tsp45I Tsp509I TspRI