##################################
# NO CUT ENZYMES (  213 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcyI        AfaI        AflII       AflIII      
AgeI        AhdI        Alw44I      AocI        Aor51HI     
ApaI        ApaLI       AscI        AseI        AsnI        
Asp700I     Asp718I     AspEI       AspHI       AspI        
AvaI        AviII       AvrII       BalI        BamHI       
BanI        BanII       BbeI        BbrPI       BbvCI       
BcgI        BciVI       BclI        BcnI        BfrI        
BglI        BglII       BlnI        BlpI        BmyI        
BpmI        Bpu1102I    BsaAI       BsaBI       BsaHI       
BsaI        BsaJI       BsaWI       BseAI       BseRI       
BsiEI       BsiHKAI     BsiWI       BsiYI       BslI        
BsmBI       BsmFI       BsoBI       Bsp1286I    Bsp1407I    
BspDI       BspEI       BspLU11I    BsrBI       BsrDI       
BsrFI       BsrGI       BssHII      BssKI       BssSI       
Bst1107I    BstAPI      BstBI       BstEII      BstNI       
BstPI       BstUI       BstXI       BstZ17I     Bsu36I      
Cac8I       CelII       CfoI        Cfr10I      ClaI        
CpoI        DraI        DraII       DraIII      DrdI        
DsaI        EaeI        EagI        Eam1105I    EarI        
EclXI       Eco47III    Eco52I      Eco81I      EcoNI       
EcoO109I    EcoO65I     EcoRII      EcoRV       EcoT14I     
EcoT22I     FauI        FbaI        FseI        FspI        
HaeII       HaeIII      HapII       HhaI        Hin1I       
HinP1I      HincII      HindII      HindIII     HinfI       
HpaI        HpaII       HphI        KasI        KpnI        
Ksp632I     KspI        MaeII       MamI        MfeI        
MluI        MluNI       MroI        MscI        MslI        
MspA1I      MspI        MunI        MvaI        MvnI        
NaeI        NarI        NciI        NcoI        NdeI        
NgoMIV      NheI        NlaIV       NotI        NruI        
NsiI        NspI        NspV        PacI        PaeR7I      
PflFI       PflMI       PinAI       PleI        PmaCI       
PmeI        PmlI        PpuMI       PshAI       PshBI       
Psp1406I    PstI        PvuI        PvuII       RsaI        
RsrII       SacI        SacII       SalI        SapI        
SbfI        ScaI        ScrFI       SexAI       SfaNI       
SfcI        SfiI        SfoI        SfuI        SgrAI       
SmaI        SmlI        SnaBI       SpeI        SphI        
Sse8387I    SspBI       SspI        StuI        StyI        
SwaI        TaqI        TfiI        Tsp45I      Tth111I     
TthHB8I     Van91I      XbaI        XcmI        XhoI        
XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   28 enzymes) #
###################################

AciI          226  CCGC                 CCGC(-3/-1)
AlwNI          44  CAGTTACTG            CAG[ACGT][ACGT][ACGT]^CTG
AvaII          67  GGACC                G^G[AT]CC
BbsI          272  GTCTTC               GAAGAC(2/6)
BbvI          258  GCAGC                GCAGC(8/12)
BfaI          131  CTAG                 C^TAG
BpuAI         272  GTCTTC               GAAGAC(2/6)
BsgI          256  GTGCAG               GTGCAG(16/14)
BsmAI         328  GAGAC                GTCTC(1/5)
BsmI            3  GAATGC               GAATGC(1/-1)
BspHI          81  TCATGA               T^CATGA
BspMI         261  GCAGGT               ACCTGC(4/8)
BstYI          19  GGATCT               [GA]^GATC[CT]
Cfr13I         67  GGACC                G^G[ACGT]CC
EcoRI         244  GAATTC               G^AATTC
Fnu4HI        258  GCAGC                GC^[ACGT]GC
HgaI          270  GCGTC                GACGC(5/10)
ItaI          258  GCAGC                GC^[ACGT]GC
MaeI          131  CTAG                 C^TAG
MaeIII         46  GTTAC                ^GT[ACGT]AC
MflI           19  GGATCT               [GA]^GATC[CT]
MseI          353  TTAA                 T^TAA
MwoI          261  GCAGGTTCAGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
RcaI           81  TCATGA               T^CATGA
Sau96I         67  GGACC                G^G[ACGT]CC
Tru9I         353  TTAA                 T^TAA
TseI          258  GCAGC                G^C[AT]GC
XhoII          19  GGATCT               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (    6 enzymes) #
###################################

AlwI          358  GATCC                GGATC(4/5)
               19  GGATC               
BsrI          303  ACTGG                ACTGG(1/-1)
               43  CCAGT               
BstF5I        136  CATCC                GGATG(2/0)
              306  GGATG               
DdeI           23  CTGAG                C^T[ACGT]AG
              199  CTGAG               
FokI          136  CATCC                GGATG(9/13)
              306  GGATG               
NlaIII         82  CATG                 CATG^
              378  CATG                


#########################################
# 3 OR MORE CUT ENZYMES (   11 enzymes) #
#########################################

AcsI        AluI        ApoI        DpnII       MboI        
MboII       MnlI        NdeII       Sau3AI      Tsp509I     
TspRI